##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754850
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48183347684971	34.0	31.0	34.0	31.0	34.0
2	32.65030668344704	34.0	31.0	34.0	31.0	34.0
3	32.7172179903292	34.0	31.0	34.0	31.0	34.0
4	36.134066370802145	37.0	37.0	37.0	35.0	37.0
5	36.012821090282834	37.0	35.0	37.0	35.0	37.0
6	35.99902762138173	37.0	35.0	37.0	35.0	37.0
7	36.19514738027423	37.0	36.0	37.0	35.0	37.0
8	36.224954626747035	37.0	37.0	37.0	35.0	37.0
9	38.033541763264225	39.0	39.0	39.0	35.0	39.0
10	37.51927402795258	39.0	37.0	39.0	35.0	39.0
11	37.38798966682122	39.0	37.0	39.0	34.0	39.0
12	37.31271908326158	39.0	37.0	39.0	34.0	39.0
13	37.22784526727165	39.0	37.0	39.0	34.0	39.0
14	38.46615751473803	40.0	38.0	41.0	34.0	41.0
15	38.542399152149436	40.0	38.0	41.0	34.0	41.0
16	38.65458435450752	40.0	38.0	41.0	35.0	41.0
17	38.660743194012056	40.0	38.0	41.0	35.0	41.0
18	38.21600582897264	39.0	38.0	40.0	35.0	41.0
19	37.777800887593564	38.0	37.0	40.0	34.0	41.0
20	37.249189905279195	38.0	35.0	40.0	34.0	41.0
21	37.180197390209976	38.0	35.0	40.0	34.0	41.0
22	37.00654434655892	38.0	35.0	40.0	34.0	41.0
23	36.89778234086242	38.0	35.0	40.0	33.0	41.0
24	36.679434324700274	37.0	35.0	40.0	33.0	41.0
25	36.553161555275885	37.0	35.0	40.0	33.0	41.0
26	36.68712989335629	37.0	35.0	40.0	33.0	41.0
27	36.72018944161091	37.0	35.0	40.0	33.0	41.0
28	36.70029542293171	38.0	35.0	40.0	33.0	41.0
29	36.57519374710208	38.0	35.0	40.0	33.0	41.0
30	36.27853878253958	38.0	35.0	40.0	33.0	41.0
31	35.77108299662184	38.0	35.0	40.0	31.0	41.0
32	35.08650592833013	38.0	35.0	40.0	24.0	41.0
33	34.24600516658939	38.0	35.0	40.0	18.0	41.0
34	33.56174869179307	38.0	34.0	40.0	12.0	41.0
35	33.11508909054779	38.0	33.0	40.0	10.0	41.0
36	32.9396794065046	38.0	33.0	40.0	10.0	41.0
37	32.711863284096175	37.0	33.0	40.0	10.0	41.0
38	32.607559117705506	37.0	32.0	40.0	10.0	41.0
39	32.569189905279195	37.0	32.0	40.0	9.0	41.0
40	32.439614492945616	37.0	32.0	40.0	8.0	41.0
41	32.31848976617871	37.0	31.0	40.0	8.0	41.0
42	32.19034245214281	37.0	31.0	40.0	8.0	41.0
43	32.03702589918527	37.0	31.0	40.0	8.0	41.0
44	31.907820096707955	37.0	31.0	40.0	7.0	41.0
45	31.857321322116977	37.0	30.0	40.0	7.0	41.0
46	31.808800423925284	36.0	30.0	40.0	7.0	41.0
47	31.78670861760615	36.0	30.0	40.0	8.0	41.0
48	31.74969464131947	36.0	30.0	40.0	7.0	41.0
49	31.65716632443532	36.0	29.0	40.0	7.0	41.0
50	31.554308803073457	36.0	29.0	40.0	7.0	41.0
51	30.969215075842882	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	3.0
11	3.0
12	7.0
13	10.0
14	18.0
15	29.0
16	46.0
17	89.0
18	221.0
19	485.0
20	814.0
21	1353.0
22	2131.0
23	3755.0
24	7682.0
25	16574.0
26	29005.0
27	34785.0
28	31604.0
29	24277.0
30	19715.0
31	17849.0
32	18624.0
33	23174.0
34	42893.0
35	60307.0
36	55871.0
37	71502.0
38	132608.0
39	159290.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.8802411075048	15.608266543021793	19.53209246870239	34.97939988077101
2	48.97449824468437	18.172087169636352	19.17890971716235	13.674504868516923
3	23.800622640259654	18.14519440948533	44.43452341524806	13.619659535006956
4	22.04994369742333	19.680731271113466	43.665363979598595	14.603961051864609
5	19.177319997350466	22.708617606146916	43.0966417168974	15.01742067960522
6	19.830297410081474	25.360667682320994	42.30257667086176	12.506458236735776
7	60.79009074650593	4.098430151685766	30.573358945485857	4.5381201563224485
8	62.00529906603961	3.2610452407763133	30.123865668675897	4.609790024508181
9	58.17433927270319	4.567265019540305	32.26468834867855	4.993707359077963
10	30.468569914552564	20.838312247466384	37.91534742001722	10.777770417963833
11	22.617738623567597	18.778830231171757	43.72590580910114	14.877525336159502
12	20.141882493210574	17.09465456713254	47.02960853149632	15.73385440816056
13	16.7088825594489	21.282109028283767	47.28634828111545	14.722660131151885
14	13.84341259852951	23.842352785321587	46.0965754785719	16.217659137577
15	12.647280916738424	20.50420613366894	51.643637808836196	15.20487514075644
16	14.681327416042922	20.143737166324435	48.590580910114596	16.58435450751805
17	14.597469695966087	19.128038683182087	45.484135921043915	20.790355699807908
18	16.455189772802544	18.823607339206465	48.16930515996556	16.551897728025438
19	16.67973769623104	19.992448830893554	45.933629197853875	17.394184275021527
20	18.35795191097569	20.244021991124065	45.63595416307876	15.762071934821487
21	16.339140226535072	21.184473736503943	46.424852619725776	16.051533417235213
22	15.948201629462808	19.80247731337352	44.957806186659596	19.291514870504074
23	14.976220441147248	20.272107041134	45.35934291581109	19.39232960190766
24	16.017089487977742	18.593097966483406	47.5334172352123	17.856395310326555
25	14.71060475591177	20.844406173411937	45.52016957011327	18.924819500563025
26	15.109889381996421	21.25548122143472	45.89309134265086	17.741538053917996
27	15.434192223620588	20.909982115652117	46.568192356097235	17.08763330463006
28	13.943432470027158	19.93561634761873	48.299794661190965	17.821156521163147
29	15.431807643902761	19.726435715705108	46.28111545340134	18.560641186990793
30	15.65675299728423	20.21527455785918	45.726965622309066	18.401006822547526
31	18.596409882758163	22.764390276213817	41.02417698880572	17.615022852222296
32	18.40723322514407	23.38451347949924	40.880572299132275	17.327680996224416
33	18.224945353381468	22.78174471749354	39.522421673180105	19.47088825594489
34	17.84367755183149	24.042392528316885	38.304033914022654	19.80989600582897
35	17.22951579784063	24.173941842750217	37.66615883950454	20.930383519904616
36	21.354308803073458	23.787242498509638	36.37994303504007	18.47850566337683
37	19.083923958402334	26.564350533218523	37.33311253891502	17.01861296946413
38	17.78989203152944	27.43988871961317	34.75485195734252	20.01536729151487
39	18.804265748161885	25.88421540703451	35.4516791415513	19.8598397032523
40	19.15413658342717	25.054514141882496	35.94396237663112	19.847386898059217
41	17.991918924289592	25.23057561104855	34.37053719281977	22.406968271842086
42	20.917268331456583	23.50056302576671	34.92614426707293	20.656024375703783
43	20.95197721401603	24.354639994700932	34.732065973372194	19.96131681791084
44	18.506855666688747	25.695303702722395	34.18930913426508	21.608531496323774
45	18.364045836921242	25.74776445651454	32.91051202225608	22.97767768430814
46	19.87533947141816	24.738424852619726	35.34861230708088	20.037623368881235
47	17.449691991786448	24.854606875538188	37.53924620785586	20.1564549248195
48	18.319798635490496	22.852619725773334	37.21693051599655	21.610651122739615
49	18.684506855666687	21.551434059746967	38.74942041465192	21.014638669934424
50	17.992316354242565	22.229979466119097	38.04888388421541	21.72882029542293
51	17.16698681857323	22.540637212691266	35.00099357488243	25.291382393853084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	482.5
2	606.0
3	26088.0
4	51570.0
5	34798.0
6	18026.0
7	17969.0
8	17912.0
9	18062.5
10	18213.0
11	17617.5
12	17022.0
13	15999.0
14	14976.0
15	13850.5
16	12725.0
17	11837.0
18	10949.0
19	9736.0
20	8523.0
21	7771.0
22	7019.0
23	6443.0
24	5867.0
25	5974.5
26	6135.5
27	6189.0
28	6489.5
29	6790.0
30	7915.5
31	9041.0
32	10042.0
33	11043.0
34	12772.5
35	14502.0
36	16118.5
37	17735.0
38	19627.5
39	21520.0
40	24126.5
41	26733.0
42	31243.0
43	35753.0
44	41836.5
45	47920.0
46	53023.5
47	58127.0
48	63966.0
49	69805.0
50	69053.5
51	68302.0
52	57149.5
53	45997.0
54	39462.0
55	32927.0
56	27575.0
57	22223.0
58	21326.0
59	20429.0
60	17298.5
61	14168.0
62	13038.0
63	11908.0
64	10358.5
65	8809.0
66	7195.5
67	5582.0
68	4333.0
69	3084.0
70	2796.5
71	2509.0
72	2182.5
73	1856.0
74	1473.5
75	805.5
76	520.0
77	396.5
78	273.0
79	199.5
80	126.0
81	75.5
82	25.0
83	17.0
84	9.0
85	6.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	754850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.51315463524979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20391087556118	25.20320294348225
2	10.610861824843736	7.11206906298514
3	4.401919322198245	4.425666088101712
4	2.360386084028484	3.1641593533175314
5	1.3764325152482166	2.3064297864249648
6	0.8603037864383739	1.7298896296920079
7	0.5769195349245727	1.3534075510216517
8	0.43729788839030875	1.1724185404234095
9	0.3155761932515744	0.9518358387279136
>10	2.9404555377053514	24.602799289069168
>50	0.8478355761061861	18.343411509192016
>100	0.06245936983487219	3.7090339950619127
>500	0.0036266730871861276	0.8043330229233117
>1k	0.0016118547054160568	1.3311468446868842
>5k	0.0	0.0
>10k+	4.029636763540142E-4	3.7901965448901382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28066	3.71808968669272	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3596	0.47638603696098564	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3205	0.42458766642379275	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1818	0.2408425515002981	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1238	0.16400609392594556	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	924	0.12240842551500299	RNA PCR Primer, Index 41 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC	851	0.11273762999271378	No Hit
ACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	830	0.10995562032191826	TruSeq Adapter, Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2571371795721004	0.0
2	0.0	0.0	0.0	1.5216268132741604	0.0
3	0.0	0.0	0.0	2.1171093594753922	0.0
4	0.0	0.0	0.0	2.749155461349937	0.0
5	0.0	0.0	0.0	4.278598397032523	0.0
6	0.0	0.0	0.0	5.006822547525998	0.0
7	0.0	0.0	0.0	5.878386434390938	0.0
8	0.0	0.0	0.0	7.199973504669802	0.0
9	0.0	0.0	0.0	7.651983837848579	0.0
10	0.0	0.0	0.0	8.642114327349805	0.0
11	0.0	0.0	0.0	10.160031794396238	0.0
12	0.0	0.0	0.0	11.356693382791283	0.0
13	0.0	0.0	0.0	11.79890044379678	0.0
14	0.0	0.0	0.0	11.963568920977679	0.0
15	0.0	0.0	0.0	12.308538120156323	0.0
16	0.0	0.0	0.0	13.080876995429556	0.0
17	0.0	0.0	0.0	14.092998608995165	0.0
18	0.0	0.0	0.0	15.315625620984301	0.0
19	0.0	0.0	0.0	15.940253030403392	0.0
20	0.0	0.0	0.0	16.51228720937935	0.0
21	0.0	0.0	0.0	17.250314632046102	0.0
22	0.0	0.0	0.0	18.0394780419951	0.0
23	0.0	0.0	0.0	18.802411075048024	0.0
24	0.0	0.0	0.0	19.4408160561701	0.0
25	0.0	0.0	0.0	20.007551169106446	0.0
26	1.3247665099026297E-4	0.0	0.0	20.532821090282837	0.0
27	1.3247665099026297E-4	0.0	0.0	21.012121613565608	0.0
28	1.3247665099026297E-4	0.0	0.0	21.49062727694244	0.0
29	1.3247665099026297E-4	0.0	0.0	22.037490892230245	0.0
30	1.3247665099026297E-4	0.0	0.0	22.622640259654236	0.0
31	1.3247665099026297E-4	0.0	0.0	23.166854341922235	0.0
32	1.3247665099026297E-4	0.0	0.0	23.66655626945751	0.0
33	1.3247665099026297E-4	0.0	0.0	24.14585679274028	0.0
34	1.3247665099026297E-4	0.0	0.0	24.62568722262701	0.0
35	1.3247665099026297E-4	0.0	0.0	25.179969530370272	0.0
36	1.3247665099026297E-4	0.0	0.0	25.694243889514475	0.0
37	1.3247665099026297E-4	0.0	0.0	26.169040206663574	0.0
38	1.3247665099026297E-4	0.0	0.0	26.652579982778036	0.0
39	1.3247665099026297E-4	0.0	0.0	27.106842419023646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCAAC	30	2.1645083E-6	45.000004	19
ACGATAC	35	1.2111923E-7	45.000004	45
ACGGCAT	60	0.0	45.000004	45
TATTCGG	30	2.1645083E-6	45.000004	1
AACGATA	35	1.2111923E-7	45.000004	44
GTATACG	30	2.1645083E-6	45.000004	1
CACACGG	65	0.0	45.000004	2
TGCGTAG	30	2.1645083E-6	45.000004	1
TCACGGC	50	2.1827873E-11	45.0	43
AACCGGA	20	7.0317474E-4	45.0	10
GATACGT	20	7.0317474E-4	45.0	9
GCCTTAC	25	3.889529E-5	45.0	35
CATGCGA	25	3.889529E-5	45.0	43
TCCGAAC	25	3.889529E-5	45.0	16
TAGCGAG	20	7.0317474E-4	45.0	1
CTCCCGA	20	7.0317474E-4	45.0	14
TCCCTCG	25	3.889529E-5	45.0	12
GAATCGG	40	6.8102963E-9	45.0	2
CCTACGG	20	7.0317474E-4	45.0	2
TAACGCG	40	6.8102963E-9	45.0	1
>>END_MODULE