##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934942.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722164
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4322134584388	34.0	31.0	34.0	30.0	34.0
2	32.59966157271755	34.0	31.0	34.0	31.0	34.0
3	32.66550672700384	34.0	31.0	34.0	31.0	34.0
4	36.12401061254784	37.0	35.0	37.0	35.0	37.0
5	35.998010147279565	37.0	35.0	37.0	35.0	37.0
6	35.95265618336001	37.0	35.0	37.0	35.0	37.0
7	36.14466797015636	37.0	35.0	37.0	35.0	37.0
8	36.05112966029877	37.0	36.0	37.0	35.0	37.0
9	37.91787876438039	39.0	38.0	39.0	35.0	39.0
10	37.47682797813239	39.0	37.0	39.0	34.0	39.0
11	37.32236998798057	39.0	37.0	39.0	34.0	39.0
12	37.291065464354354	39.0	37.0	39.0	34.0	39.0
13	37.19337851236007	39.0	37.0	39.0	33.0	39.0
14	38.445494375238866	40.0	38.0	41.0	34.0	41.0
15	38.4610559374325	40.0	38.0	41.0	34.0	41.0
16	38.627109631607226	40.0	38.0	41.0	34.0	41.0
17	38.5576406467229	40.0	38.0	41.0	34.0	41.0
18	38.13799774012551	39.0	38.0	40.0	34.0	41.0
19	37.66584044621443	38.0	37.0	40.0	34.0	41.0
20	36.998314787222846	38.0	35.0	40.0	34.0	41.0
21	37.0099797275965	38.0	35.0	40.0	34.0	41.0
22	36.86567594064506	38.0	35.0	40.0	33.0	41.0
23	36.638748262167596	37.0	35.0	40.0	33.0	41.0
24	36.45285142986911	37.0	35.0	40.0	33.0	41.0
25	36.347038899751304	37.0	35.0	40.0	33.0	41.0
26	36.52852122232623	37.0	35.0	40.0	33.0	41.0
27	36.47095673558915	37.0	35.0	40.0	33.0	41.0
28	36.4603414182928	37.0	35.0	40.0	33.0	41.0
29	36.351659733800076	37.0	35.0	40.0	33.0	41.0
30	36.001243484859394	37.0	35.0	40.0	31.0	41.0
31	35.543557972981205	37.0	35.0	40.0	30.0	41.0
32	34.653199273295265	37.0	35.0	40.0	21.0	41.0
33	33.69687910225378	37.0	34.0	40.0	15.0	41.0
34	32.977442796927015	37.0	33.0	40.0	10.0	41.0
35	32.42524551209974	37.0	31.0	40.0	9.0	41.0
36	32.25636282063354	37.0	31.0	40.0	8.0	41.0
37	32.047858935089536	37.0	31.0	40.0	8.0	41.0
38	31.922370818816777	37.0	30.0	40.0	8.0	41.0
39	31.937900532289063	37.0	30.0	40.0	8.0	41.0
40	31.77375222248686	37.0	30.0	40.0	7.0	41.0
41	31.607644523958548	37.0	29.0	40.0	7.0	41.0
42	31.473270614431073	37.0	28.0	40.0	7.0	41.0
43	31.389088905013267	37.0	27.0	40.0	7.0	41.0
44	31.34434283625326	37.0	27.0	40.0	7.0	41.0
45	31.30391573105278	37.0	26.0	40.0	7.0	41.0
46	31.21500379415202	36.0	26.0	40.0	7.0	41.0
47	31.067726998299555	36.0	26.0	40.0	7.0	41.0
48	31.017814513046897	36.0	25.0	40.0	7.0	41.0
49	30.9460731911311	36.0	25.0	40.0	7.0	41.0
50	30.846687179089514	36.0	24.0	40.0	7.0	41.0
51	30.200160905279134	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	2.0
11	2.0
12	7.0
13	10.0
14	9.0
15	21.0
16	58.0
17	134.0
18	265.0
19	554.0
20	1232.0
21	2075.0
22	3267.0
23	5185.0
24	9375.0
25	18542.0
26	31418.0
27	36530.0
28	31595.0
29	24384.0
30	20063.0
31	18088.0
32	18951.0
33	22676.0
34	37762.0
35	54309.0
36	51200.0
37	66833.0
38	121112.0
39	146407.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.392326396774145	13.403742086285108	17.578278618153217	34.625652898787536
2	48.285707955533645	23.080629884624546	17.133365828260615	11.500296331581191
3	20.79901518214699	23.297339662458942	43.99956242626329	11.904082729130778
4	19.760469920959782	18.089935250164782	49.45123268398868	12.698362144886758
5	22.377880924554532	20.837372120460174	42.6447455148692	14.140001440116096
6	17.984557524329652	27.95251494120449	43.00657468386682	11.056352850599033
7	55.6880431591716	3.802460382960103	36.760071119579486	3.7494253382888094
8	55.10133432295158	8.229986540453416	32.0675082114312	4.601170925163813
9	51.31645997308091	5.17680194526451	32.81276829085914	10.693969790795443
10	29.854575968893492	20.58119762270066	39.37512808724888	10.189098321156967
11	22.57991259603082	17.35159880581142	46.386139436471495	13.682349161686266
12	18.938357492203988	15.508942567062329	48.44882879789078	17.103871142842898
13	16.59318381974178	20.19153544070322	51.2058202845891	12.009460454965907
14	13.991835649520054	24.874266787045602	45.70845403537147	15.425443528062877
15	11.459446884641162	19.165175777247274	54.786031981655135	14.589345356456429
16	13.166815294032935	19.611888712259265	47.87112068726771	19.350175306440086
17	12.459081316709224	20.86022011620629	48.309525260190206	18.371173306894278
18	13.483225416941305	19.360699231753454	49.22981483430357	17.926260517001676
19	14.775868085365651	20.467926952880507	46.102824289219626	18.65338067253422
20	16.559258007876327	20.56001129937244	49.19630444054259	13.68442625220864
21	15.705850748583424	23.617350075606094	47.12738380755618	13.549415368254303
22	13.992528013027512	18.615854570429985	47.643333093313984	19.748284323228518
23	13.528921408433542	22.481873923374746	46.95415445799015	17.03505021020156
24	15.472662719271524	18.699907500235405	47.65538021834376	18.17204956214932
25	12.775491439617594	24.513268454256927	45.34163984912015	17.36960025700533
26	14.138616713101179	20.615677325372076	47.61923884325444	17.626467118272306
27	16.19701342077423	21.660038440021935	46.60395699591782	15.538991143286013
28	13.172908092898567	20.37625802449305	49.71820805246453	16.732625830143846
29	16.391013675564	17.595726178541163	47.118106136556236	18.8951540093386
30	14.91960274951396	22.81310062534272	47.39408223062905	14.873214394514264
31	20.28029644235935	19.976764280689704	41.5051151815931	18.23782409535784
32	18.547172110490138	22.870428323760255	41.41441556211608	17.167984003633524
33	16.881760929650326	24.483912241540704	39.54974216382982	19.084584664979147
34	21.409264377620595	22.509568463673073	37.56501293335032	18.51615422535601
35	16.8789914756205	23.16856005007173	39.817825313917616	20.13462316039016
36	21.92673686309481	26.400512902886327	35.434195002797146	16.238555231221717
37	17.77851014451011	23.7462681606948	40.07801552002038	18.397206174774706
38	17.870179072897567	27.576838502057704	34.344414842058036	20.20856758298669
39	19.520496729274793	26.17383309054453	34.83377736912945	19.47189281105123
40	19.167806758575615	24.885621548567915	37.92074930348231	18.025822389374156
41	19.938545815078015	26.03688358876931	34.01858857544824	20.005982020704437
42	18.85015038135382	23.12452573099739	38.150890933361396	19.874432954287393
43	20.53107050476069	25.7657540392487	33.57173716773475	20.131438288255854
44	18.82550224048831	23.924482527514527	35.84753601674966	21.402479215247507
45	18.143524185642043	23.26798344974272	34.559462947474536	24.029029417140706
46	22.12655297134723	24.7668119706881	34.1283974277311	18.978237630233576
47	16.28563594972887	23.947468995962137	40.74794644983688	19.018948604472115
48	18.64091812939997	23.581901064024237	36.1247583651359	21.652422441439896
49	17.97652610764314	21.03857295572751	40.616535856121324	20.368365080508028
50	18.455918600207156	22.060086074631247	37.76482904160274	21.71916628355886
51	17.718274519361252	21.993480705213774	34.51016666574351	25.778078109681456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	528.0
2	656.0
3	23960.5
4	47265.0
5	32495.0
6	17725.0
7	17585.5
8	17446.0
9	17754.0
10	18062.0
11	17843.0
12	17624.0
13	16621.0
14	15618.0
15	14516.5
16	13415.0
17	12333.0
18	11251.0
19	10505.5
20	9760.0
21	8703.0
22	7646.0
23	7256.5
24	6867.0
25	6815.5
26	7297.0
27	7830.0
28	8026.5
29	8223.0
30	9323.0
31	10423.0
32	11167.0
33	11911.0
34	13122.0
35	14333.0
36	15622.5
37	16912.0
38	18980.0
39	21048.0
40	23065.5
41	25083.0
42	29259.5
43	33436.0
44	38245.5
45	43055.0
46	63600.5
47	84146.0
48	75212.0
49	66278.0
50	64262.5
51	62247.0
52	50356.5
53	38466.0
54	32999.5
55	27533.0
56	23316.5
57	19100.0
58	16351.5
59	13603.0
60	11656.5
61	9710.0
62	8095.0
63	6480.0
64	5645.0
65	4810.0
66	3919.5
67	3029.0
68	2452.0
69	1875.0
70	1517.0
71	1159.0
72	830.5
73	502.0
74	370.0
75	165.0
76	92.0
77	72.5
78	53.0
79	66.5
80	80.0
81	43.0
82	6.0
83	3.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.31455332710512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.04907769208864	26.439046852694414
2	10.03302135554701	6.885572926738048
3	4.035446825117103	4.1542366583753365
4	2.0895359750657336	2.868059745811909
5	1.2420553769827347	2.1310287734345854
6	0.7810790562486551	1.6081427356997648
7	0.5406252807062559	1.29859205173432
8	0.3780513799549909	1.0378131390280974
9	0.27497493003661316	0.8492077710322525
>10	2.872861970045734	24.07050891044678
>50	0.6297389909913805	13.880782463704447
>100	0.0653153943419927	3.919964654290067
>500	0.002053943218301657	0.4025825830721144
>1k	0.004518675080263645	3.046010867508866
>5k	0.0012323659309809941	3.7947919183768883
>10k+	4.107886436603314E-4	3.613657948052075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25636	3.549886175439374	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	9761	1.3516320392597803	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	9724	1.34650854930459	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	7436	1.0296830082917454	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	4464	0.6181421394586271	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	3287	0.455159769802981	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	3037	0.42054159443007405	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	1970	0.272791221938507	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1686	0.2334649747148847	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	1608	0.2226641039985377	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	1310	0.1813992389540326	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	1229	0.17018295013321072	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	1010	0.13985742850654423	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	1005	0.13916506499908607	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1003	0.1388881195961028	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3847270149162794E-4	0.0	0.0	0.28968489152048565	0.0
2	1.3847270149162794E-4	0.0	0.0	1.6890900127948776	0.0
3	1.3847270149162794E-4	0.0	0.0	2.2559972527016026	0.0
4	1.3847270149162794E-4	0.0	0.0	3.0041652588608683	0.0
5	1.3847270149162794E-4	0.0	0.0	5.469117818113337	0.0
6	1.3847270149162794E-4	0.0	0.0	6.142787510870107	0.0
7	1.3847270149162794E-4	0.0	0.0	7.098664569266814	0.0
8	1.3847270149162794E-4	0.0	0.0	8.407785489168665	0.0
9	1.3847270149162794E-4	0.0	0.0	8.78166178319606	0.0
10	1.3847270149162794E-4	0.0	0.0	11.082662663882441	0.0
11	1.3847270149162794E-4	0.0	0.0	12.3899834386649	0.0
12	1.3847270149162794E-4	0.0	0.0	14.9305420929318	0.0
13	1.3847270149162794E-4	0.0	0.0	15.386394226242238	0.0
14	1.3847270149162794E-4	0.0	0.0	15.585933389091675	0.0
15	1.3847270149162794E-4	0.0	0.0	16.118083980924002	0.0
16	1.3847270149162794E-4	0.0	0.0	16.826925739859643	0.0
17	1.3847270149162794E-4	0.0	0.0	17.73087553519699	0.0
18	1.3847270149162794E-4	0.0	0.0	18.720262987354673	0.0
19	1.3847270149162794E-4	0.0	0.0	19.77819442675071	0.0
20	2.769454029832559E-4	0.0	0.0	20.40339867398541	0.0
21	2.769454029832559E-4	0.0	0.0	21.01669426889183	0.0
22	2.769454029832559E-4	0.0	0.0	21.74312206091691	0.0
23	2.769454029832559E-4	0.0	0.0	22.467195817016634	0.0
24	2.769454029832559E-4	0.0	0.0	23.004746844207133	0.0
25	2.769454029832559E-4	0.0	0.0	23.48843199051739	0.0
26	2.769454029832559E-4	0.0	0.0	23.90440398579824	0.0
27	2.769454029832559E-4	0.0	0.0	24.367456699586242	0.0
28	2.769454029832559E-4	0.0	0.0	24.809738508150502	0.0
29	2.769454029832559E-4	0.0	0.0	25.265036750654975	0.0
30	2.769454029832559E-4	0.0	0.0	25.82585119169607	0.0
31	4.154181044748838E-4	0.0	0.0	26.314798300663007	0.0
32	4.154181044748838E-4	0.0	0.0	26.763034435391408	0.0
33	4.154181044748838E-4	0.0	0.0	27.201023590209427	0.0
34	4.154181044748838E-4	0.0	0.0	27.636658709102086	0.0
35	4.154181044748838E-4	0.0	0.0	28.115912728964613	0.0
36	4.154181044748838E-4	0.0	0.0	28.57148791687207	0.0
37	4.154181044748838E-4	0.0	0.0	28.986075185138002	0.0
38	4.154181044748838E-4	0.0	0.0	29.41547903246354	0.0
39	4.154181044748838E-4	0.0	0.0	29.849729424341284	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	60	0.0	45.000004	2
GCGACAA	30	2.1643773E-6	45.000004	11
ATTAGCG	30	2.1643773E-6	45.000004	1
GCGAAGA	30	2.1643773E-6	45.000004	12
CTGAGCG	30	2.1643773E-6	45.000004	1
CTGCGAA	30	2.1643773E-6	45.000004	10
CATCGAA	30	2.1643773E-6	45.000004	42
TTACGAG	30	2.1643773E-6	45.000004	1
GCGCTTC	55	1.8189894E-12	45.000004	25
TAGTGCG	30	2.1643773E-6	45.000004	1
TGTTTCG	20	7.0315425E-4	45.0	16
AACCGTC	20	7.0315425E-4	45.0	19
AACCGCG	20	7.0315425E-4	45.0	15
GGTGTCG	20	7.0315425E-4	45.0	12
CACGGTG	20	7.0315425E-4	45.0	43
GCGAACC	20	7.0315425E-4	45.0	33
CGTGATA	20	7.0315425E-4	45.0	22
TAGCGGC	20	7.0315425E-4	45.0	13
TAGCGAC	20	7.0315425E-4	45.0	29
ATAGCGA	20	7.0315425E-4	45.0	28
>>END_MODULE