##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721486
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.928835763964926	34.0	33.0	34.0	31.0	34.0
2	33.07032430289708	34.0	33.0	34.0	31.0	34.0
3	33.10761816584105	34.0	33.0	34.0	31.0	34.0
4	36.43283168349767	37.0	37.0	37.0	35.0	37.0
5	36.33854156560211	37.0	37.0	37.0	35.0	37.0
6	36.34060813376836	37.0	37.0	37.0	35.0	37.0
7	36.45456876502108	37.0	37.0	37.0	35.0	37.0
8	36.47959904973901	37.0	37.0	37.0	35.0	37.0
9	38.3023953340744	39.0	39.0	39.0	37.0	39.0
10	37.82633758659212	39.0	38.0	39.0	35.0	39.0
11	37.747370011337715	39.0	38.0	39.0	35.0	39.0
12	37.67522446728003	39.0	37.0	39.0	35.0	39.0
13	37.63712532190507	39.0	37.0	39.0	35.0	39.0
14	38.99932639025567	41.0	38.0	41.0	35.0	41.0
15	39.04526629761354	41.0	38.0	41.0	35.0	41.0
16	39.13605946615735	41.0	39.0	41.0	35.0	41.0
17	39.14067909841632	41.0	39.0	41.0	35.0	41.0
18	38.59780924369981	39.0	38.0	41.0	35.0	41.0
19	38.0504916242311	38.0	37.0	41.0	35.0	41.0
20	37.45463252232199	38.0	35.0	41.0	35.0	41.0
21	37.40968501121297	38.0	35.0	41.0	35.0	41.0
22	37.240987351105915	38.0	35.0	41.0	35.0	41.0
23	37.16295118685602	38.0	35.0	40.0	34.0	41.0
24	36.99335260836662	37.0	35.0	40.0	34.0	41.0
25	36.85655023105092	37.0	35.0	40.0	34.0	41.0
26	36.982183992482184	38.0	35.0	40.0	34.0	41.0
27	36.99433946050235	38.0	35.0	40.0	34.0	41.0
28	36.91971985596394	38.0	35.0	40.0	34.0	41.0
29	36.753476851941684	38.0	35.0	40.0	34.0	41.0
30	36.358547497803144	38.0	35.0	40.0	33.0	41.0
31	35.768034306972	38.0	35.0	41.0	31.0	41.0
32	34.94006536509371	38.0	35.0	41.0	21.0	41.0
33	33.9065234807051	38.0	35.0	41.0	15.0	41.0
34	33.09027895205174	38.0	33.0	41.0	10.0	41.0
35	32.56151747920264	38.0	33.0	41.0	8.0	41.0
36	32.33383322753318	37.0	32.0	41.0	7.0	41.0
37	32.1362618262863	37.0	31.0	41.0	7.0	41.0
38	32.043002913431444	37.0	31.0	41.0	7.0	41.0
39	31.976211596621418	37.0	31.0	41.0	7.0	41.0
40	31.858149707686636	37.0	30.0	41.0	7.0	41.0
41	31.720507397232932	37.0	29.0	41.0	7.0	41.0
42	31.572395029148176	37.0	29.0	40.0	7.0	41.0
43	31.460481561665784	36.0	28.0	40.0	7.0	41.0
44	31.351602110089456	36.0	27.0	40.0	7.0	41.0
45	31.283821169087133	36.0	26.0	40.0	7.0	41.0
46	31.219018802859654	36.0	25.0	40.0	7.0	41.0
47	31.16878220783217	36.0	25.0	40.0	7.0	41.0
48	31.098783621581013	35.0	25.0	40.0	7.0	41.0
49	30.955562547298214	35.0	24.0	40.0	7.0	41.0
50	30.8458778132909	35.0	24.0	40.0	7.0	41.0
51	30.297621852676283	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	8.0
11	8.0
12	3.0
13	21.0
14	18.0
15	27.0
16	40.0
17	74.0
18	140.0
19	261.0
20	503.0
21	879.0
22	1614.0
23	3273.0
24	7934.0
25	18085.0
26	32229.0
27	38403.0
28	33649.0
29	24793.0
30	18717.0
31	15490.0
32	14191.0
33	17251.0
34	37146.0
35	51104.0
36	45994.0
37	58345.0
38	121920.0
39	179239.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.072081786756776	14.457106582802714	17.81517590084908	38.655635729591424
2	53.278511294744455	17.141427553687805	17.450373257415944	12.129687894151791
3	23.37023864634934	17.43900782551567	46.711370698807734	12.47938282932725
4	21.35855720000111	18.535494798235863	46.12397191352292	13.981976088240106
5	18.776802321874577	21.50686222601686	45.696382188982184	14.01995326312638
6	19.974746564728907	24.990644309106482	44.345143218302226	10.689465907862385
7	59.4618606598049	4.051915075275196	33.254144917572894	3.2320793473470033
8	60.402973862278685	3.1762224076420056	33.09835533884233	3.322448391236975
9	55.99983922071946	4.933844870170731	34.66761655804825	4.398699351061559
10	30.253948101557064	17.104559201425946	41.35076772106458	11.29072497595241
11	24.389939652328664	17.856479543608607	44.23661720393743	13.516963600125298
12	21.47248872465994	16.719936353581357	47.17901664065553	14.62855828110317
13	18.464530150273188	18.9431257155371	48.3594969271753	14.232847207014412
14	16.265596283226564	20.399564232708602	48.2998977111129	15.034941772951935
15	15.278051133355325	18.254685468602304	52.17620300324608	14.291060394796295
16	17.501933509451327	18.024881979691916	49.364228827725	15.108955683131759
17	17.552662144518397	17.476569191917793	47.37555545083342	17.595213212730393
18	17.703323418611035	17.783297250397098	48.69851944459075	15.814859886401123
19	17.751279997117063	19.287415140418528	47.76295035523905	15.198354507225364
20	19.005081179676388	19.628793905910857	46.96515247697114	14.400972437441615
21	17.584956603454536	19.26662471621071	47.904741048336355	15.243677631998404
22	17.03109970255833	18.02793124190906	47.67646219053454	17.264506864998072
23	16.49609278627721	18.658435506718078	47.88214878736386	16.963322919640852
24	16.452294292612745	18.411722472785335	48.76920688689732	16.366776347704597
25	15.834125679500364	19.376259553199922	47.71194451451588	17.07767025278384
26	14.85406508234394	20.270109191307938	47.9048796511644	16.97094607518372
27	15.12350898007723	20.208015124340598	48.892147595379534	15.776328300202639
28	14.282467019457066	19.519436274577746	49.43851994356093	16.75957676240426
29	15.079156075100556	18.756427706150916	48.685074970269696	17.479341248478832
30	16.94946263683564	19.85291467887111	47.26619781950031	15.931424864792943
31	17.001577300183232	20.53719684096434	45.28681083208822	17.174415026764205
32	17.623072381168868	21.25335765350956	43.40139656209545	17.72217340322612
33	18.56709624303174	23.301907452119654	40.32552260196317	17.805473702885433
34	17.461738689316217	22.599052511067434	40.241945096647754	19.697263702968595
35	17.986350393493428	24.362080483890196	37.95375100833557	19.697818114280803
36	19.397742991548	26.56073714528071	36.824276562538984	17.21724330063231
37	18.44373972606537	26.093507011917072	37.33682982067566	18.1259234413419
38	19.299196380802954	26.511671744150267	34.89880607523916	19.29032579980762
39	19.596499446974715	25.85774360140044	35.16797276731635	19.377784184308496
40	20.120695342667773	24.338518003121333	35.518776525116216	20.022010129094674
41	18.720252368029318	25.28087863104759	34.696030137798935	21.302838863124162
42	18.62822009020272	25.670491180702047	34.99846150860862	20.702827220486608
43	18.616577452646343	25.789689612826862	35.12777794718123	20.46595498734556
44	18.278109346543108	25.249554391907814	35.375461200910344	21.096875060638737
45	18.468272426630595	24.57622185323069	34.7302650363278	22.225240683810913
46	19.83004521224251	23.943084134688682	36.14941939275329	20.077451260315517
47	17.453006711148937	24.440668287395738	37.46683927338854	20.639485728066795
48	18.243042831045926	23.391444879041313	37.7577666094699	20.607745680442864
49	18.569036682624475	22.700371178373523	38.45327560063536	20.277316538366648
50	18.14061534111542	22.884158528370612	37.861718730508976	21.11350740000499
51	17.704709446891552	22.952905531084454	35.868055651807516	23.47432937021647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	327.0
1	506.0
2	685.0
3	28104.5
4	55524.0
5	37618.0
6	19712.0
7	19341.5
8	18971.0
9	19261.0
10	19551.0
11	19187.5
12	18824.0
13	17760.0
14	16696.0
15	15612.5
16	14529.0
17	13124.5
18	11720.0
19	10768.0
20	9816.0
21	9111.5
22	8407.0
23	7701.5
24	6996.0
25	6511.0
26	6135.0
27	6244.0
28	6097.5
29	5951.0
30	7116.0
31	8281.0
32	9286.0
33	10291.0
34	11474.5
35	12658.0
36	13671.0
37	14684.0
38	16848.5
39	19013.0
40	21055.5
41	23098.0
42	26341.5
43	29585.0
44	32965.0
45	36345.0
46	41206.5
47	46068.0
48	48929.0
49	51790.0
50	52118.0
51	52446.0
52	46019.0
53	39592.0
54	36218.0
55	32844.0
56	30865.5
57	28887.0
58	27269.5
59	25652.0
60	23376.5
61	21101.0
62	18844.5
63	16588.0
64	14247.0
65	11906.0
66	9935.0
67	7964.0
68	6342.0
69	4720.0
70	3841.5
71	2963.0
72	2676.5
73	2390.0
74	1800.0
75	1012.5
76	815.0
77	568.5
78	322.0
79	253.5
80	185.0
81	113.0
82	41.0
83	36.0
84	31.0
85	25.5
86	20.0
87	14.5
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.96784944299539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46260130439151	27.436684293177354
2	9.512163700026392	6.652398162792976
3	3.4629562076713145	3.6327639389261037
4	1.616020617310004	2.2603506257149073
5	0.9325431214535499	1.6304513735044344
6	0.6001735307462521	1.2592066597683542
7	0.4069738019264182	0.9961699043104493
8	0.29976768789900504	0.838578510266179
9	0.2521858848099456	0.7936558246514479
>10	3.850538326605725	34.6176044190206
>50	0.5498949875400023	11.925577433400765
>100	0.05013748412586981	3.029784090490634
>500	0.002830341845815231	0.6120647406661046
>1k	8.086690988043517E-4	0.4344825474860105
>5k	0.0	0.0
>10k+	4.0433454940217583E-4	3.8802274758236486
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27444	3.803816013061931	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1708	0.2367336303129929	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1365	0.18919286029112137	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	797	0.11046645395752655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3860282805210357E-4	0.0	0.0	0.10339770972686926	0.0
2	1.3860282805210357E-4	0.0	0.0	0.6805398857358286	0.0
3	1.3860282805210357E-4	0.0	0.0	1.0000194043959274	0.0
4	1.3860282805210357E-4	0.0	0.0	1.3250430361781103	0.0
5	1.3860282805210357E-4	0.0	0.0	2.184934981413361	0.0
6	1.3860282805210357E-4	0.0	0.0	2.6302658679447695	0.0
7	1.3860282805210357E-4	0.0	0.0	3.1037331285707555	0.0
8	1.3860282805210357E-4	0.0	0.0	3.8061722611388165	0.0
9	1.3860282805210357E-4	0.0	0.0	4.047202579121424	0.0
10	1.3860282805210357E-4	0.0	0.0	4.68269654574032	0.0
11	1.3860282805210357E-4	0.0	0.0	5.683131758620403	0.0
12	1.3860282805210357E-4	0.0	0.0	6.431171221617606	0.0
13	1.3860282805210357E-4	0.0	0.0	6.724870614260014	0.0
14	1.3860282805210357E-4	0.0	0.0	6.8406039756835195	0.0
15	1.3860282805210357E-4	0.0	0.0	7.074426946607419	0.0
16	1.3860282805210357E-4	0.0	0.0	7.6033353384542455	0.0
17	1.3860282805210357E-4	0.0	0.0	8.233146589123004	0.0
18	1.3860282805210357E-4	0.0	0.0	8.973562896577342	0.0
19	1.3860282805210357E-4	0.0	0.0	9.37343205550766	0.0
20	2.7720565610420714E-4	0.0	0.0	9.790903773600597	0.0
21	2.7720565610420714E-4	0.0	0.0	10.275875068954907	0.0
22	2.7720565610420714E-4	0.0	0.0	10.792170603448993	0.0
23	2.7720565610420714E-4	0.0	0.0	11.31054518036386	0.0
24	2.7720565610420714E-4	0.0	0.0	11.739105124700965	0.0
25	2.7720565610420714E-4	0.0	0.0	12.143132368472846	0.0
26	2.7720565610420714E-4	0.0	0.0	12.50183648747169	0.0
27	2.7720565610420714E-4	0.0	0.0	12.867054939388984	0.0
28	2.7720565610420714E-4	0.0	0.0	13.230194348885494	0.0
29	2.7720565610420714E-4	0.0	0.0	13.619529692883853	0.0
30	2.7720565610420714E-4	0.0	0.0	14.041298098646404	0.0
31	2.7720565610420714E-4	0.0	0.0	14.442691888685296	0.0
32	2.7720565610420714E-4	0.0	0.0	14.840482005194835	0.0
33	2.7720565610420714E-4	0.0	0.0	15.23882653301658	0.0
34	2.7720565610420714E-4	0.0	0.0	15.61180674330479	0.0
35	2.7720565610420714E-4	0.0	0.0	16.03856485087722	0.0
36	2.7720565610420714E-4	0.0	0.0	16.42887041467194	0.0
37	2.7720565610420714E-4	0.0	0.0	16.827630750977843	0.0
38	2.7720565610420714E-4	0.0	0.0	17.229163143844787	0.0
39	2.7720565610420714E-4	0.0	0.0	17.65564404576111	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	25	3.8893573E-5	45.0	15
CTACTCG	20	7.0315396E-4	45.0	43
GTCGACG	20	7.0315396E-4	45.0	1
CCTAGCG	20	7.0315396E-4	45.0	45
GGCCGAT	75	0.0	45.0	8
ATAGCGC	20	7.0315396E-4	45.0	43
CACGCGA	25	3.8893573E-5	45.0	11
CGATTAC	25	3.8893573E-5	45.0	10
CGTTATG	20	7.0315396E-4	45.0	1
GCCGATG	45	3.8380676E-10	45.0	9
TACGAAC	20	7.0315396E-4	45.0	36
TCATGCG	20	7.0315396E-4	45.0	1
GTATAGT	20	7.0315396E-4	45.0	9
GACGATT	25	3.8893573E-5	45.0	20
TCGAAGT	20	7.0315396E-4	45.0	14
TGCGTAG	55	1.8189894E-12	45.0	1
ACGGTTG	20	7.0315396E-4	45.0	1
AACGTCG	30	2.1643737E-6	44.999996	1
TAGAACG	30	2.1643737E-6	44.999996	1
CGATTTA	30	2.1643737E-6	44.999996	10
>>END_MODULE