##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1264702
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.621389861010734	34.0	31.0	34.0	31.0	34.0
2	32.77954253254917	34.0	31.0	34.0	31.0	34.0
3	32.83097441136331	34.0	31.0	34.0	31.0	34.0
4	36.21300828179287	37.0	37.0	37.0	35.0	37.0
5	36.10679274643355	37.0	35.0	37.0	35.0	37.0
6	36.110090756557675	37.0	36.0	37.0	35.0	37.0
7	36.247838621272045	37.0	37.0	37.0	35.0	37.0
8	36.27508377467577	37.0	37.0	37.0	35.0	37.0
9	38.068163883665875	39.0	39.0	39.0	37.0	39.0
10	37.60139938103996	39.0	38.0	39.0	35.0	39.0
11	37.51856801048785	39.0	37.0	39.0	35.0	39.0
12	37.47345303478606	39.0	37.0	39.0	35.0	39.0
13	37.43621975769786	39.0	37.0	39.0	35.0	39.0
14	38.75191942449684	40.0	38.0	41.0	35.0	41.0
15	38.79798798452126	40.0	38.0	41.0	35.0	41.0
16	38.87880623261448	40.0	38.0	41.0	35.0	41.0
17	38.87561022280347	40.0	38.0	41.0	35.0	41.0
18	38.35785347061996	39.0	38.0	41.0	35.0	41.0
19	37.87995037566162	38.0	37.0	41.0	35.0	41.0
20	37.30648405711385	38.0	35.0	41.0	34.0	41.0
21	37.24973472011588	38.0	35.0	41.0	34.0	41.0
22	37.076160233794205	38.0	35.0	40.0	34.0	41.0
23	36.97385629183792	38.0	35.0	40.0	34.0	41.0
24	36.77029292275967	37.0	35.0	40.0	33.0	41.0
25	36.62268265567699	37.0	35.0	40.0	33.0	41.0
26	36.7334486701215	37.0	35.0	40.0	33.0	41.0
27	36.78317738091661	38.0	35.0	40.0	33.0	41.0
28	36.723712779769464	38.0	35.0	40.0	33.0	41.0
29	36.5978475561832	38.0	35.0	40.0	33.0	41.0
30	36.2830318920979	38.0	35.0	40.0	33.0	41.0
31	35.766832819114704	38.0	35.0	40.0	31.0	41.0
32	35.04886763838438	38.0	35.0	40.0	23.0	41.0
33	34.09895848982606	38.0	34.0	40.0	17.0	41.0
34	33.31518650243299	38.0	33.0	40.0	12.0	41.0
35	32.805702845413386	38.0	33.0	41.0	10.0	41.0
36	32.57787684371496	38.0	32.0	41.0	8.0	41.0
37	32.359744825263185	37.0	31.0	40.0	8.0	41.0
38	32.20775091681677	37.0	31.0	40.0	8.0	41.0
39	32.14100159563281	37.0	31.0	40.0	8.0	41.0
40	32.00479401471651	37.0	30.0	40.0	7.0	41.0
41	31.881198100422075	37.0	30.0	40.0	7.0	41.0
42	31.761955780887515	37.0	29.0	40.0	7.0	41.0
43	31.660952540598498	37.0	29.0	40.0	7.0	41.0
44	31.552243137118467	37.0	28.0	40.0	7.0	41.0
45	31.5133335758147	36.0	27.0	40.0	7.0	41.0
46	31.463927470661073	36.0	27.0	40.0	7.0	41.0
47	31.46031950609709	36.0	27.0	40.0	7.0	41.0
48	31.421709620131857	36.0	27.0	40.0	7.0	41.0
49	31.3146314309616	36.0	27.0	40.0	7.0	41.0
50	31.22191235563793	35.0	27.0	40.0	7.0	41.0
51	30.674639559358646	35.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	10.0
12	15.0
13	23.0
14	21.0
15	53.0
16	94.0
17	198.0
18	414.0
19	746.0
20	1420.0
21	2142.0
22	3491.0
23	6121.0
24	11877.0
25	26303.0
26	48842.0
27	63510.0
28	59925.0
29	47898.0
30	37525.0
31	32115.0
32	31036.0
33	36719.0
34	65175.0
35	89019.0
36	87108.0
37	109751.0
38	211206.0
39	291728.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.18244930426298	15.821276474616155	18.983128041230266	37.0131461798906
2	50.513796926074285	18.146330123618053	18.693415523973236	12.646457426334425
3	22.88428420291895	17.989771503484615	46.39559358647333	12.730350707123101
4	21.529893998744367	19.117388918496214	46.01653195772601	13.336185125033406
5	18.3852006243368	21.983834927121173	44.95383102106267	14.677133427479358
6	19.60635786137762	24.664782691891055	44.20480081473738	11.52405863199394
7	59.11613961233555	4.704270254969154	32.56253251754168	3.6170576151536094
8	60.924391674876766	3.5583876676086543	31.819037211928187	3.6981834455863916
9	56.663625106942185	5.045061998794973	33.63108463495749	4.660228259305354
10	29.757840186858246	18.62699671543178	39.69757302510789	11.917590072602083
11	23.153359447522025	19.62288349350282	43.336216753037476	13.887540305937684
12	21.452721668820008	16.937586878173672	46.59239884178249	15.017292611223828
13	17.582640021127506	19.073267852822244	47.39401060486976	15.950081521180485
14	14.213941307912853	21.408363393115533	46.84652985446374	17.531165444507877
15	13.858679752226216	19.74014431858256	51.31137611864297	15.089799810548254
16	16.05627254483665	18.867843966404735	49.41258889445893	15.663294594299684
17	15.842546307351455	18.334595817829022	46.732906249851744	19.089951624967778
18	16.138663495432127	19.805535216991828	47.77070013331204	16.28510115426401
19	18.217730342800124	19.964703147460824	46.12960207226683	15.687964437472226
20	19.197091488745965	20.24168539308074	46.004829596221086	14.556393521952208
21	17.770273155257126	20.05207550869691	46.78825525696963	15.389396079076336
22	16.43746906385852	19.475576064559082	46.060415813369474	18.026539058212922
23	15.963839702949786	19.91148903061749	46.83182283257242	17.292848433860307
24	17.277113501836798	18.139688242763906	48.00419387333933	16.579004382059964
25	16.065444666016184	19.71840006578625	46.13679744319215	18.079357825005417
26	14.954352883129781	22.125291175312444	46.165104506832435	16.755251434725334
27	16.23750100814263	21.451456548657312	46.1406718736904	16.170370569509654
28	14.198048235869004	21.158423090973212	47.39630363516465	17.24722503799314
29	16.310324487507728	20.2655645361516	46.198234841093	17.22587613524767
30	16.937745018194008	21.091608932380908	45.37124160474167	16.59940444468341
31	16.90904260450288	22.189337883548852	43.440114746398756	17.461504765549513
32	18.30352130383284	23.37254151570884	41.47071800313433	16.853219177323986
33	17.73002652008141	24.126553132674733	39.86994564727501	18.273474699968848
34	16.419441101540126	24.319404887475468	39.74169409078186	19.519459920202546
35	17.860650176879613	26.24776429546249	36.262060153300936	19.629525374356962
36	18.595605921394924	27.740527017431777	34.66959014850929	18.99427691266401
37	18.48712186744387	26.735072768130358	36.74525698544005	18.032548378985723
38	18.287074741717813	27.63275459357224	34.0414579877315	20.038712676978452
39	18.913309222251566	26.719337836106842	35.21635926882381	19.150993672817787
40	18.48728000746421	26.73649602831339	36.452618877806785	18.323605086415615
41	16.858200587964596	27.675136119022504	34.55304095352107	20.913622339491834
42	18.07706479471053	27.43033536754113	33.614242722791616	20.878357114956724
43	19.3381523868864	26.121726699254054	33.722647706732495	20.817473207127055
44	18.175348817349857	26.62176544355904	33.665480089380736	21.53740564971037
45	18.0573763621786	26.028503157265504	34.154053682211305	21.76006679834459
46	18.431535650295487	26.7309611276016	35.282461797324586	19.555041424778327
47	16.930075227207674	25.55835287680418	37.48717089085017	20.02440100513797
48	17.228801725623903	23.80529168136051	37.35725886414349	21.6086477288721
49	17.881840939604746	23.25899698110701	38.316457157496394	20.542704921791852
50	16.964628821651267	23.15454549767455	38.20670798338265	21.674117697291535
51	16.06568187604669	23.654030751908355	36.72177319242003	23.558514179624922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	396.0
1	737.0
2	1078.0
3	53366.5
4	105655.0
5	72336.5
6	39018.0
7	36552.0
8	34086.0
9	33266.5
10	32447.0
11	30960.0
12	29473.0
13	27558.0
14	25643.0
15	23317.0
16	20991.0
17	19132.0
18	17273.0
19	15683.0
20	14093.0
21	13094.5
22	12096.0
23	11141.0
24	10186.0
25	10629.5
26	11890.5
27	12708.0
28	13825.5
29	14943.0
30	16037.0
31	17131.0
32	19592.5
33	22054.0
34	23309.0
35	24564.0
36	25763.5
37	26963.0
38	29441.0
39	31919.0
40	36301.5
41	40684.0
42	47209.5
43	53735.0
44	59318.5
45	64902.0
46	73735.0
47	82568.0
48	90325.0
49	98082.0
50	96178.5
51	94275.0
52	81429.0
53	68583.0
54	61347.0
55	54111.0
56	47802.0
57	41493.0
58	40086.0
59	38679.0
60	38418.0
61	38157.0
62	35074.5
63	31992.0
64	26573.0
65	21154.0
66	16821.5
67	12489.0
68	10089.0
69	7689.0
70	6401.5
71	5114.0
72	4134.5
73	3155.0
74	2581.5
75	1516.0
76	1024.0
77	746.0
78	468.0
79	411.0
80	354.0
81	219.0
82	84.0
83	81.5
84	79.0
85	46.5
86	14.0
87	10.5
88	7.0
89	6.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1264702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.46007177937985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14322295420237	25.040745543861124
2	9.904407211398786	6.42995538028424
3	3.797247645366688	3.697767933980515
4	1.8981872516957048	2.464611777629854
5	1.0895861691728577	1.768402263058524
6	0.6810068555354468	1.3263318847758223
7	0.4902518173763251	1.1139526427404856
8	0.3564621903060677	0.9256630627175932
9	0.2895061190966371	0.8457650465801878
>10	3.4875980728216764	29.169359411318084
>50	0.769669057071269	15.840785970614006
>100	0.08565469600796073	4.946779969775364
>500	0.004717215138512345	0.9864225147460154
>1k	0.002234470328769006	1.271630789230178
>5k	0.0	0.0
>10k+	2.4827448097433397E-4	4.171825808688091
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51766	4.093138146377566	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	3399	0.26875896456240284	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	3337	0.26385662393196185	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1875	0.1482562690657562	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	1502	0.11876315527294178	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5814002033680662E-4	0.0	0.0	0.15932607048933267	0.0
2	1.5814002033680662E-4	0.0	0.0	0.9319982098549698	0.0
3	1.5814002033680662E-4	0.0	0.0	1.3437948228120142	0.0
4	1.5814002033680662E-4	0.0	0.0	1.7697449675892027	0.0
5	1.5814002033680662E-4	0.0	0.0	2.8542692270590226	0.0
6	1.5814002033680662E-4	0.0	0.0	3.4159825792953598	0.0
7	1.5814002033680662E-4	0.0	0.0	4.009561145629563	0.0
8	1.5814002033680662E-4	0.0	0.0	4.880201027593852	0.0
9	1.5814002033680662E-4	0.0	0.0	5.173787975349134	0.0
10	1.5814002033680662E-4	0.0	0.0	5.881701776386849	0.0
11	1.5814002033680662E-4	0.0	0.0	6.981012127758159	0.0
12	1.5814002033680662E-4	0.0	0.0	7.8173356253093615	0.0
13	1.5814002033680662E-4	0.0	0.0	8.139466846735436	0.0
14	1.5814002033680662E-4	0.0	0.0	8.268430033320103	0.0
15	1.5814002033680662E-4	0.0	0.0	8.532049447221558	0.0
16	1.5814002033680662E-4	0.0	0.0	9.102618640596758	0.0
17	1.5814002033680662E-4	0.0	0.0	9.774397446987512	0.0
18	1.5814002033680662E-4	0.0	0.0	10.569209189200302	0.0
19	2.3721003050520992E-4	0.0	0.0	11.01492683651959	0.0
20	2.3721003050520992E-4	0.0	0.0	11.467365434703195	0.0
21	2.3721003050520992E-4	0.0	0.0	12.032478797376774	0.0
22	3.1628004067361324E-4	0.0	0.0	12.631750404443101	0.0
23	3.1628004067361324E-4	0.0	0.0	13.240747622760145	0.0
24	3.1628004067361324E-4	0.0	0.0	13.707023472723218	0.0
25	3.9535005084201657E-4	0.0	0.0	14.137164328039333	0.0
26	3.9535005084201657E-4	0.0	0.0	14.536705089420275	0.0
27	3.9535005084201657E-4	0.0	0.0	14.900822486245772	0.0
28	4.7442006101041983E-4	0.0	0.0	15.294274856843746	0.0
29	4.7442006101041983E-4	0.0	0.0	15.67989929643505	0.0
30	4.7442006101041983E-4	0.0	0.0	16.121663443245918	0.0
31	4.7442006101041983E-4	0.0	0.0	16.542394967351992	0.0
32	4.7442006101041983E-4	0.0	0.0	16.943517128936303	0.0
33	4.7442006101041983E-4	0.0	0.0	17.332146228914006	0.0
34	4.7442006101041983E-4	0.0	0.0	17.724966039430633	0.0
35	4.7442006101041983E-4	0.0	0.0	18.149176643984116	0.0
36	4.7442006101041983E-4	0.0	0.0	18.557652316514087	0.0
37	4.7442006101041983E-4	0.0	0.0	18.953555857427283	0.0
38	4.7442006101041983E-4	0.0	0.0	19.335701216571177	0.0
39	4.7442006101041983E-4	0.0	0.0	19.717925645725238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGA	30	2.1656742E-6	45.000004	12
GATTACC	30	2.1656742E-6	45.000004	21
GTCGCGA	20	7.0335553E-4	45.0	17
CGAAACG	20	7.0335553E-4	45.0	1
ACCGCGT	20	7.0335553E-4	45.0	16
TTAAGCG	40	6.8157533E-9	45.0	1
CGAATGT	20	7.0335553E-4	45.0	13
ACGCTAG	35	1.2120654E-7	45.0	1
TCGAGTA	20	7.0335553E-4	45.0	39
TTGCACG	35	1.2120654E-7	45.0	1
CGTCCGC	25	3.8910286E-5	45.0	41
TATCGTG	20	7.0335553E-4	45.0	1
TATCGTA	20	7.0335553E-4	45.0	25
AGGTGCG	25	3.8910286E-5	45.0	1
GCGTAAG	70	0.0	45.0	1
ACGACCG	20	7.0335553E-4	45.0	44
CGTTTTT	34515	0.0	44.511078	1
ATGCGCG	100	0.0	42.75	1
ACGGGCC	130	0.0	41.538464	5
TATCGCG	55	6.184564E-11	40.909092	1
>>END_MODULE