##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934934.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	550887
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70074080528312	34.0	31.0	34.0	31.0	34.0
2	32.84820661950636	34.0	31.0	34.0	31.0	34.0
3	32.894363090797206	34.0	31.0	34.0	31.0	34.0
4	36.27322844793942	37.0	37.0	37.0	35.0	37.0
5	36.16545316916174	37.0	37.0	37.0	35.0	37.0
6	36.172993735557384	37.0	37.0	37.0	35.0	37.0
7	36.264039630632055	37.0	37.0	37.0	35.0	37.0
8	36.262881498383514	37.0	37.0	37.0	35.0	37.0
9	38.02867738755861	39.0	39.0	39.0	37.0	39.0
10	37.68151363165222	39.0	38.0	39.0	35.0	39.0
11	37.53538384459971	39.0	37.0	39.0	35.0	39.0
12	37.385801443853275	39.0	37.0	39.0	35.0	39.0
13	37.28469722465769	39.0	37.0	39.0	34.0	39.0
14	38.61566891213624	40.0	38.0	41.0	35.0	41.0
15	38.69115081677368	40.0	38.0	41.0	35.0	41.0
16	38.79492890556502	40.0	38.0	41.0	35.0	41.0
17	38.796885749709105	40.0	38.0	41.0	35.0	41.0
18	38.31872961242505	39.0	38.0	41.0	35.0	41.0
19	37.80679703823107	38.0	37.0	41.0	35.0	41.0
20	37.21310541000241	38.0	35.0	41.0	34.0	41.0
21	37.15820485870968	38.0	35.0	41.0	34.0	41.0
22	36.9566716949211	38.0	35.0	41.0	34.0	41.0
23	36.868562881316855	37.0	35.0	40.0	33.0	41.0
24	36.677376667084175	37.0	35.0	40.0	33.0	41.0
25	36.51426336072552	37.0	35.0	40.0	33.0	41.0
26	36.5653645847515	37.0	35.0	40.0	33.0	41.0
27	36.593684367211424	37.0	35.0	40.0	33.0	41.0
28	36.51486057939287	37.0	35.0	40.0	33.0	41.0
29	36.34647032876071	37.0	35.0	40.0	33.0	41.0
30	36.043832945776536	37.0	35.0	40.0	32.0	41.0
31	35.48968300214018	37.0	35.0	40.0	30.0	41.0
32	34.70591064955245	37.0	35.0	40.0	22.0	41.0
33	33.74791200373216	37.0	34.0	40.0	15.0	41.0
34	32.94884794885339	37.0	33.0	40.0	10.0	41.0
35	32.451246807421484	36.0	32.0	40.0	10.0	41.0
36	32.219142945830995	36.0	31.0	40.0	8.0	41.0
37	31.96073423406252	36.0	31.0	40.0	8.0	41.0
38	31.846271558413974	36.0	30.0	40.0	7.0	41.0
39	31.748701639356167	36.0	30.0	40.0	7.0	41.0
40	31.584021768529663	36.0	29.0	40.0	7.0	41.0
41	31.431219106640746	36.0	29.0	40.0	7.0	41.0
42	31.292985675828255	36.0	27.0	40.0	7.0	41.0
43	31.191747127813873	35.0	27.0	40.0	7.0	41.0
44	31.100688525959043	35.0	26.0	40.0	7.0	41.0
45	31.01637540911294	35.0	25.0	40.0	7.0	41.0
46	30.977469063528456	35.0	25.0	40.0	7.0	41.0
47	30.942853979128206	35.0	25.0	40.0	7.0	41.0
48	30.88007340888422	35.0	25.0	40.0	7.0	41.0
49	30.75400944295291	35.0	24.0	40.0	7.0	41.0
50	30.66283466482237	35.0	24.0	40.0	7.0	41.0
51	30.150139683819006	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	2.0
10	1.0
11	4.0
12	9.0
13	17.0
14	16.0
15	30.0
16	66.0
17	120.0
18	247.0
19	454.0
20	764.0
21	1200.0
22	2000.0
23	3304.0
24	6150.0
25	12584.0
26	22864.0
27	28713.0
28	27152.0
29	20886.0
30	15875.0
31	13460.0
32	13324.0
33	16225.0
34	30927.0
35	46198.0
36	35733.0
37	45292.0
38	82874.0
39	124304.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.747843024068455	15.18369465970335	17.587272888995383	37.481189427232806
2	50.660480279984824	17.982453751858365	18.4175702094985	12.939495758658309
3	23.80633414838252	18.432273769393724	45.28750905358086	12.473883028642897
4	21.40547880055256	19.249501258878862	45.59664686224217	13.74837307832641
5	19.40525007850975	21.962035771401396	44.38078952670874	14.251924623380113
6	20.986699631684903	25.1042409786399	42.66628183275336	11.242777556921837
7	60.518037274431954	4.393460001778949	31.92923412605489	3.1592685977341994
8	61.569614095086656	4.153483382254437	31.32166850914979	2.9552340135091226
9	57.58113732943416	5.65651394932173	32.35418515956993	4.408163561674173
10	33.98755098595538	18.57495275800663	37.34341162525164	10.09408463078635
11	27.1108230907609	17.551149328265144	41.903148921648175	13.434878659325777
12	24.57255299181139	16.50265843993414	44.91683412387658	14.007954444377885
13	18.438627159471906	19.84762755338209	46.33454773846542	15.379197548680581
14	15.224356356203723	22.787976481565185	45.59773601482699	16.389931147404095
15	14.83752566315778	18.48600529691207	51.66122998001404	15.0152390599161
16	16.280652838059346	16.894934895904242	51.423068614797586	15.401343651238818
17	15.754229088724184	17.177025415375567	46.91960420195067	20.149141293949576
18	18.012950024233646	18.230962066630724	46.92450538858242	16.83158252055322
19	19.15528956029095	19.547747541691855	45.15318023478499	16.143782663232205
20	21.545253382272588	18.03382544877625	45.455601602506505	14.96531956644466
21	19.549199745138296	18.649196659205973	44.73821309996424	17.063390495691493
22	18.672068863487432	18.2231564731061	44.839685815784364	18.265088847622106
23	17.9385245976035	19.87322263912563	44.840774968369196	17.34747779490168
24	17.67023001087337	17.710528656512135	47.505386767159145	17.113854565455348
25	17.0635720211223	19.23897278389216	46.37484638410418	17.32260881088136
26	16.085512999943727	22.0802088268556	45.68650195049075	16.14777622270992
27	16.90328506572128	21.13010472202103	45.477203128772324	16.48940708348536
28	15.62770586345294	20.570643344279315	47.259238282987255	16.54241250928049
29	16.41298487711636	19.51543601500852	46.99493725573484	17.076641852140277
30	17.230393892032303	20.642527414878188	44.82171479813464	17.305363894954866
31	18.623601573462434	22.02193916356712	41.64865026765925	17.705808995311198
32	19.82838585771674	22.935193605948225	39.97262596503457	17.263794571300465
33	19.571708898558143	24.056294666601318	38.10618148549521	18.26581494934533
34	17.935983241572227	24.747906557969237	37.54726468404591	19.768845516412622
35	18.77208937586111	26.26836356639383	35.32702713986716	19.632519917877893
36	20.293817062301343	27.287810385795996	34.00007624068094	18.41829631122172
37	20.246257399430373	27.730369386099145	34.59965473863061	17.423718475839873
38	19.305411091566874	27.2324451294004	33.049427559553955	20.412716219478767
39	19.76013229573397	27.693156672784074	33.561510799855505	18.98520023162645
40	19.975239931238168	27.006445968047892	33.94344030626971	19.074873794444233
41	17.751916454735724	27.408887848143088	33.35239350356788	21.486802193553306
42	19.13586633919479	27.450275646366677	31.870419886473993	21.54343812796454
43	20.075623494473458	27.469154291170423	31.377578341837797	21.07764387251832
44	19.653032291558887	27.84037379716711	31.668926658280196	20.837667252993807
45	18.230962066630724	27.734181420146054	32.352551430692685	21.682305082530537
46	19.557549914955334	27.724197521451764	33.162880953807225	19.555371609785674
47	18.865393447295	26.2707233969943	35.26748679856305	19.596396357147654
48	19.311582956214252	24.04050195412126	35.107925944885245	21.539989144779238
49	17.697095774632547	24.74445757478394	36.564667527097214	20.993779123486306
50	17.728681199592657	25.047968095090283	36.205065648672054	21.018285056645013
51	17.368897795736693	25.995167793031964	33.732326230243224	22.903608180988115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	302.0
2	495.0
3	19494.0
4	38493.0
5	27581.0
6	16669.0
7	16326.0
8	15983.0
9	15513.0
10	15043.0
11	14507.0
12	13971.0
13	12967.0
14	11963.0
15	10855.5
16	9748.0
17	9006.0
18	8264.0
19	7570.0
20	6876.0
21	6286.5
22	5697.0
23	5394.0
24	5091.0
25	4818.0
26	4685.0
27	4825.0
28	5120.0
29	5415.0
30	5477.0
31	5539.0
32	7177.0
33	8815.0
34	9028.5
35	9242.0
36	10510.5
37	11779.0
38	12982.0
39	14185.0
40	16437.0
41	18689.0
42	20907.0
43	23125.0
44	25377.5
45	27630.0
46	29812.5
47	31995.0
48	32428.5
49	32862.0
50	32568.5
51	32275.0
52	29704.5
53	27134.0
54	25134.5
55	23135.0
56	22818.5
57	22502.0
58	22358.0
59	22214.0
60	21761.5
61	21309.0
62	20218.0
63	19127.0
64	15751.5
65	12376.0
66	10028.0
67	7680.0
68	6578.0
69	5476.0
70	4794.0
71	4112.0
72	3341.5
73	2571.0
74	2283.0
75	1564.0
76	1133.0
77	793.0
78	453.0
79	289.0
80	125.0
81	124.5
82	124.0
83	102.0
84	80.0
85	44.5
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	550887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.66009019947473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68669899861199	27.495816563906217
2	7.268342660555678	4.893061391099886
3	2.4398264794692577	2.4637433811000626
4	1.138398480550714	1.5327438215312803
5	0.6920787222896843	1.1647716108703998
6	0.47861695968187384	0.9666174020334141
7	0.37164540641728805	0.8756732531558453
8	0.30834733248609525	0.8303199219399513
9	0.2716606515634056	0.8229709831745078
>10	4.703470682350801	40.565899805216986
>50	0.6045731626919778	12.335836040731282
>100	0.03413801100810801	2.243688022793204
>500	0.0016518392423278071	0.360850865717691
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.506130807759357E-4	3.448006936729288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18604	3.3770991147004743	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	767	0.13923000542761038	Illumina PCR Primer Index 9 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	609	0.11054898736038425	Illumina PCR Primer Index 9 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	571	0.10365102098978557	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8152543080522866E-4	0.0	0.0	0.08150491843154767	0.0
2	1.8152543080522866E-4	0.0	0.0	0.4650681537229958	0.0
3	1.8152543080522866E-4	0.0	0.0	0.6745485008722297	0.0
4	1.8152543080522866E-4	0.0	0.0	0.8876593566375681	0.0
5	1.8152543080522866E-4	0.0	0.0	1.490142261480122	0.0
6	1.8152543080522866E-4	0.0	0.0	1.775863289567552	0.0
7	1.8152543080522866E-4	0.0	0.0	2.084819572798051	0.0
8	1.8152543080522866E-4	0.0	0.0	2.5224773864694576	0.0
9	1.8152543080522866E-4	0.0	0.0	2.6709651888681343	0.0
10	1.8152543080522866E-4	0.0	0.0	3.0935563917827067	0.0
11	1.8152543080522866E-4	0.0	0.0	3.637769633336782	0.0
12	1.8152543080522866E-4	0.0	0.0	4.157476941732152	0.0
13	1.8152543080522866E-4	0.0	0.0	4.33065220272034	0.0
14	1.8152543080522866E-4	0.0	0.0	4.411067968567056	0.0
15	1.8152543080522866E-4	0.0	0.0	4.559192720104123	0.0
16	1.8152543080522866E-4	0.0	0.0	4.848907307669268	0.0
17	1.8152543080522866E-4	0.0	0.0	5.194350202491618	0.0
18	1.8152543080522866E-4	0.0	0.0	5.565569708488311	0.0
19	1.8152543080522866E-4	0.0	0.0	5.81897920989241	0.0
20	1.8152543080522866E-4	0.0	0.0	6.0340868453966054	0.0
21	1.8152543080522866E-4	0.0	0.0	6.295846516617746	0.0
22	1.8152543080522866E-4	0.0	0.0	6.602442969247777	0.0
23	1.8152543080522866E-4	0.0	0.0	6.899418574045131	0.0
24	1.8152543080522866E-4	0.0	0.0	7.14937909226393	0.0
25	1.8152543080522866E-4	0.0	0.0	7.3530506256273975	0.0
26	1.8152543080522866E-4	0.0	0.0	7.548735040035433	0.0
27	1.8152543080522866E-4	0.0	0.0	7.728082165670999	0.0
28	1.8152543080522866E-4	0.0	0.0	7.92703403783353	0.0
29	1.8152543080522866E-4	0.0	0.0	8.131976249212633	0.0
30	1.8152543080522866E-4	0.0	0.0	8.357612359703532	0.0
31	3.630508616104573E-4	0.0	0.0	8.595410674058382	0.0
32	3.630508616104573E-4	0.0	0.0	8.813785767317071	0.0
33	3.630508616104573E-4	0.0	0.0	9.016549673526512	0.0
34	3.630508616104573E-4	0.0	0.0	9.23801069910889	0.0
35	3.630508616104573E-4	0.0	0.0	9.470363250539584	0.0
36	3.630508616104573E-4	0.0	0.0	9.700174445939004	0.0
37	3.630508616104573E-4	0.0	0.0	9.925265980137487	0.0
38	3.630508616104573E-4	0.0	0.0	10.161612091045894	0.0
39	3.630508616104573E-4	0.0	0.0	10.379261082581364	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTT	20	7.0300826E-4	45.000004	44
CGTGAGT	25	3.8881506E-5	45.000004	32
TAGCGTA	25	3.8881506E-5	45.000004	1
CATTGCG	20	7.0300826E-4	45.000004	1
CGTGAAC	25	3.8881506E-5	45.000004	34
TAGCGCG	25	3.8881506E-5	45.000004	1
GCACGTT	20	7.0300826E-4	45.000004	34
ATCGTGA	25	3.8881506E-5	45.000004	30
TCGTGAT	20	7.0300826E-4	45.000004	44
TCTACGA	20	7.0300826E-4	45.000004	15
CGAATAT	40	6.8048394E-9	45.000004	14
CATACCA	25	3.8881506E-5	45.000004	15
TCCGTGA	20	7.0300826E-4	45.000004	36
ACGCTAG	20	7.0300826E-4	45.000004	1
ATGCGTG	20	7.0300826E-4	45.000004	12
CTCGAAT	20	7.0300826E-4	45.000004	43
ACGTATG	20	7.0300826E-4	45.000004	1
CGGTCGA	20	7.0300826E-4	45.000004	13
GCTACGA	40	6.8048394E-9	45.000004	10
ACATACC	25	3.8881506E-5	45.000004	14
>>END_MODULE