FastQCFastQC Report
Sat 14 Jan 2017
SRR2934933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086306
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415513.824981174733454No Hit
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC31150.28675161510660896Illumina PCR Primer Index 9 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC28070.25839864642191057RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT17300.15925531111859825RNA PCR Primer, Index 17 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC11740.10807267933712968Illumina PCR Primer Index 9 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAATC207.033116E-445.023
CCGATCC207.033116E-445.042
ACGTTAC207.033116E-445.038
CGCGACG207.033116E-445.01
TAGCCCG253.890664E-545.01
CAAATCG253.890664E-545.018
TAGTACG207.033116E-445.01
CCCGATC207.033116E-445.041
GCCCGAT302.1653923E-644.99999639
CCCGATA302.1653923E-644.99999640
AGGTCGT302.1653923E-644.99999614
CGTTTTT296950.044.6211471
TTGCGAG556.184564E-1140.9090921
ATCTACG451.9281288E-840.0000041
GTTTTTT333550.039.8396032
TTTAGCG403.4579898E-739.3751
TTAATCG403.4579898E-739.37520
TCTACGG750.039.02
AATACGG750.039.02
ACGGGTC356.247974E-638.5714265
Produced by FastQC (version 0.11.5)