##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934933.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086306
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.595000856112364	34.0	31.0	34.0	31.0	34.0
2	32.75668181893499	34.0	31.0	34.0	31.0	34.0
3	32.80677359786285	34.0	31.0	34.0	31.0	34.0
4	36.20152148657929	37.0	37.0	37.0	35.0	37.0
5	36.090982651297146	37.0	35.0	37.0	35.0	37.0
6	36.08663396869758	37.0	36.0	37.0	35.0	37.0
7	36.25720377131306	37.0	37.0	37.0	35.0	37.0
8	36.27813525838944	37.0	37.0	37.0	35.0	37.0
9	38.074224942143374	39.0	39.0	39.0	37.0	39.0
10	37.587973370302656	39.0	38.0	39.0	35.0	39.0
11	37.464467654601926	39.0	37.0	39.0	35.0	39.0
12	37.460406183892935	39.0	37.0	39.0	35.0	39.0
13	37.468862364748055	39.0	37.0	39.0	35.0	39.0
14	38.76392931641729	40.0	38.0	41.0	35.0	41.0
15	38.815713067956914	40.0	38.0	41.0	35.0	41.0
16	38.89771574491902	40.0	38.0	41.0	35.0	41.0
17	38.89628428822081	40.0	38.0	41.0	35.0	41.0
18	38.38859492629149	39.0	38.0	41.0	35.0	41.0
19	37.883180245713454	38.0	37.0	41.0	35.0	41.0
20	37.313590277509284	38.0	35.0	41.0	34.0	41.0
21	37.26423954208114	38.0	35.0	41.0	34.0	41.0
22	37.09067702838795	38.0	35.0	40.0	34.0	41.0
23	36.964746581534115	38.0	35.0	40.0	34.0	41.0
24	36.75552836861805	37.0	35.0	40.0	33.0	41.0
25	36.62313841587913	37.0	35.0	40.0	33.0	41.0
26	36.75998475567658	38.0	35.0	40.0	33.0	41.0
27	36.803321531870395	38.0	35.0	40.0	33.0	41.0
28	36.7547127604929	38.0	35.0	40.0	33.0	41.0
29	36.604915189642696	38.0	35.0	40.0	33.0	41.0
30	36.31384250846447	38.0	35.0	40.0	33.0	41.0
31	35.7609494930526	38.0	35.0	40.0	31.0	41.0
32	34.99288874405554	38.0	35.0	40.0	23.0	41.0
33	34.00631958214352	38.0	34.0	40.0	16.0	41.0
34	33.17132925713381	38.0	33.0	40.0	10.0	41.0
35	32.65012344587989	38.0	33.0	41.0	8.0	41.0
36	32.433355794776055	38.0	32.0	41.0	8.0	41.0
37	32.216729908515646	37.0	31.0	40.0	8.0	41.0
38	32.08072771392223	37.0	31.0	40.0	8.0	41.0
39	32.00247904365805	37.0	30.0	40.0	7.0	41.0
40	31.850973850830243	37.0	30.0	40.0	7.0	41.0
41	31.723331179244155	37.0	29.0	40.0	7.0	41.0
42	31.58162064832561	37.0	29.0	40.0	7.0	41.0
43	31.467406053174702	37.0	28.0	40.0	7.0	41.0
44	31.342780947541485	37.0	27.0	40.0	7.0	41.0
45	31.287662039977686	36.0	26.0	40.0	7.0	41.0
46	31.225729214420245	36.0	26.0	40.0	7.0	41.0
47	31.202396930514976	36.0	26.0	40.0	7.0	41.0
48	31.165610794748442	36.0	26.0	40.0	7.0	41.0
49	31.045957584695287	36.0	26.0	40.0	7.0	41.0
50	30.922843103140366	35.0	26.0	40.0	7.0	41.0
51	30.34102913911918	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	7.0
10	10.0
11	4.0
12	15.0
13	15.0
14	22.0
15	56.0
16	80.0
17	177.0
18	366.0
19	740.0
20	1233.0
21	1957.0
22	3260.0
23	5723.0
24	11648.0
25	25941.0
26	45893.0
27	56601.0
28	51503.0
29	39406.0
30	29932.0
31	25699.0
32	25462.0
33	30738.0
34	52676.0
35	74994.0
36	74951.0
37	97160.0
38	181528.0
39	248354.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.240293250704685	15.435153630744928	19.318681844710422	37.00587127383997
2	50.88593821630369	17.85629463521328	18.90222460338063	12.355542545102391
3	22.204424904216676	17.752180324880833	47.1742768612159	12.86911790968659
4	20.580112785900106	19.84302765519108	46.316599558503775	13.260260000405042
5	18.488805180124203	22.22522935526454	45.70397291370939	13.581992550901864
6	19.984608388428306	24.06099202250563	44.49473721032564	11.459662378740429
7	59.96164984820115	4.121214464432674	32.44822361286783	3.468912074498346
8	61.333546901149404	3.0461950868355694	31.978742637893927	3.6415153741211044
9	57.16372734754296	4.973828737022534	33.76451938956427	4.097924525870242
10	29.251334338574953	21.00264566337662	39.24796512216631	10.498054875882119
11	21.194580532557126	18.585923303378607	46.03758057122027	14.181915592844005
12	19.410368717470032	17.461194175490146	48.180439029150165	14.947998077889654
13	16.313359219225525	19.677972873205153	49.21854431440128	14.790123593168039
14	14.499965939615542	21.666639050138727	48.09528806800294	15.738106942242794
15	13.320924306779123	20.138616559238372	52.04187402076394	14.49858511321856
16	15.43349663906855	19.180599205012218	49.84341428658223	15.542489869337
17	15.968520840352534	18.48236132360495	47.174921246867825	18.37419658917469
18	15.987392134444622	19.10364114715375	49.00138634970257	15.907580368699058
19	16.74518966110838	19.535379533943477	47.502269158045706	16.21716164690244
20	18.201961509924462	19.94171071502873	47.151999528677926	14.704328246368886
21	17.439469173510965	19.45805325571248	48.178321762008125	14.924155808768433
22	16.402100329004902	18.721152235189717	46.92462344864155	17.952123987163837
23	15.503090289476445	19.936279464533936	47.549769586101895	17.01086065988773
24	15.66906562239369	18.777121731814056	48.78735825817035	16.766454387621906
25	14.940173394973424	20.148098233830982	47.63749808985682	17.274230281338777
26	15.211459754433834	20.8934683229219	47.3664878956758	16.52858402696846
27	15.346412520965547	21.265647064455134	46.96908605862436	16.418854355954952
28	14.846553365258039	19.725841521633868	48.57636798471149	16.851237128396605
29	15.42355468901028	19.37152146816827	47.57812255478659	17.626801288034862
30	16.416368868440383	19.92735012050012	46.42384374200271	17.232437269056785
31	17.26576121277062	21.801223596297913	44.02921460435641	16.903800586575056
32	18.306351985536303	22.60256318201317	42.524021776552836	16.567063055897695
33	18.313348172614347	23.632291453789264	40.3501407522374	17.70421962135899
34	17.506761446590556	24.650512838923838	39.32602784114237	18.516697873343237
35	17.638400229769513	25.0279387207656	37.34850033047778	19.9851607189871
36	19.888042595732696	26.09476519507395	35.60644974804521	18.410742461148146
37	18.666103289496697	26.804233797843334	36.8676045239555	17.662058388704473
38	18.31307200733495	27.609163532190745	35.15593212225653	18.921832338217776
39	19.268880039325936	26.91037332022469	35.260322597868374	18.560424042581005
40	19.496256119362315	25.81326072027587	36.35522587558202	18.335257284779797
41	17.608022049035906	26.808652442313672	34.77132594315046	20.81199956549996
42	18.773991858647562	26.225943702787248	34.89366716192307	20.106397276642124
43	19.34059095687587	26.14355439443398	34.563925818323746	19.9519288303664
44	18.824069829311448	26.516377521619138	34.07815109186546	20.581401557203954
45	18.658738882046126	26.410237999237786	33.665560164447214	21.26546295426887
46	19.339854516130814	26.067240722227435	35.16256008896204	19.430344672679706
47	17.426305295193067	25.820625127726444	36.99178684459075	19.76128273248974
48	17.83760745130746	24.4533308294348	36.93581734796641	20.77324437129133
49	18.309021583237136	24.08989732174912	37.75860577038146	19.842475324632286
50	17.565032320543196	24.184714067675223	37.048400726867015	21.20185288491456
51	16.862467849758726	24.379410589649694	35.72888302191095	23.02923853868063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	386.0
1	625.5
2	865.0
3	40814.5
4	80764.0
5	55696.5
6	30629.0
7	29922.5
8	29216.0
9	29156.0
10	29096.0
11	28099.5
12	27103.0
13	25510.0
14	23917.0
15	21831.0
16	19745.0
17	18110.5
18	16476.0
19	15222.5
20	13969.0
21	12639.0
22	11309.0
23	11096.0
24	10883.0
25	10348.0
26	10841.5
27	11870.0
28	12118.5
29	12367.0
30	13937.5
31	15508.0
32	17627.5
33	19747.0
34	21015.0
35	22283.0
36	25041.5
37	27800.0
38	29129.0
39	30458.0
40	34742.5
41	39027.0
42	44717.5
43	50408.0
44	56229.5
45	62051.0
46	67092.0
47	72133.0
48	77875.0
49	83617.0
50	82849.0
51	82081.0
52	69163.5
53	56246.0
54	51007.5
55	45769.0
56	41813.0
57	37857.0
58	33967.0
59	30077.0
60	27837.5
61	25598.0
62	22294.0
63	18990.0
64	16620.0
65	14250.0
66	11766.0
67	9282.0
68	7277.5
69	5273.0
70	4777.5
71	4282.0
72	3283.5
73	2285.0
74	1861.5
75	1042.0
76	646.0
77	542.5
78	439.0
79	309.5
80	180.0
81	139.0
82	98.0
83	71.5
84	45.0
85	30.0
86	15.0
87	12.5
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1086306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.609508514587034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.64406035602585	28.00476336869599
2	9.603699318907294	6.83966025336326
3	3.4621865826111584	3.698602877777431
4	1.7283019158041324	2.4617592714641736
5	0.9929095381229405	1.7678510326001762
6	0.6150203969445392	1.3140344436984763
7	0.43496615291963925	1.0842251648164343
8	0.32529124819850147	0.9266769177956146
9	0.2527360675839015	0.8099826435514967
>10	3.2101171136106568	29.094486373944132
>50	0.6697750984961772	15.139658392266341
>100	0.056187934611225406	3.205734542819733
>500	0.0031655174426330566	0.8262560739933594
>1k	0.0013189656010971068	0.9231974414741627
>5k	0.0	0.0
>10k+	2.6379312021942135E-4	3.903111201739208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41551	3.824981174733454	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	3115	0.28675161510660896	Illumina PCR Primer Index 9 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	2807	0.25839864642191057	RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	1730	0.15925531111859825	RNA PCR Primer, Index 17 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1174	0.10807267933712968	Illumina PCR Primer Index 9 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.205509313213772E-5	0.0	0.0	0.16818465515241562	0.0
2	9.205509313213772E-5	0.0	0.0	0.9459581370258472	0.0
3	9.205509313213772E-5	0.0	0.0	1.3348909055091291	0.0
4	9.205509313213772E-5	0.0	0.0	1.7463771718097847	0.0
5	9.205509313213772E-5	0.0	0.0	2.794608517305437	0.0
6	9.205509313213772E-5	0.0	0.0	3.3256743495847396	0.0
7	9.205509313213772E-5	0.0	0.0	3.898809359425429	0.0
8	9.205509313213772E-5	0.0	0.0	4.751239521829024	0.0
9	9.205509313213772E-5	0.0	0.0	5.06542355468901	0.0
10	9.205509313213772E-5	0.0	0.0	5.749392896660794	0.0
11	9.205509313213772E-5	0.0	0.0	6.808486743146038	0.0
12	9.205509313213772E-5	0.0	0.0	7.6169145710324715	0.0
13	9.205509313213772E-5	0.0	0.0	7.933676146500157	0.0
14	9.205509313213772E-5	0.0	0.0	8.057306136576619	0.0
15	9.205509313213772E-5	0.0	0.0	8.311746413993847	0.0
16	9.205509313213772E-5	0.0	0.0	8.844653348135793	0.0
17	9.205509313213772E-5	0.0	0.0	9.526045147499875	0.0
18	9.205509313213772E-5	0.0	0.0	10.321401152161545	0.0
19	9.205509313213772E-5	0.0	0.0	10.764002039940864	0.0
20	9.205509313213772E-5	0.0	0.0	11.207247313372108	0.0
21	9.205509313213772E-5	0.0	0.0	11.747242489685227	0.0
22	9.205509313213772E-5	0.0	0.0	12.319180783315199	0.0
23	9.205509313213772E-5	0.0	0.0	12.914869290973261	0.0
24	9.205509313213772E-5	0.0	0.0	13.366215412600132	0.0
25	9.205509313213772E-5	0.0	0.0	13.797861744296727	0.0
26	9.205509313213772E-5	0.0	0.0	14.172065697878866	0.0
27	9.205509313213772E-5	0.0	0.0	14.54857102878931	0.0
28	9.205509313213772E-5	0.0	0.0	14.935846805596213	0.0
29	9.205509313213772E-5	0.0	0.0	15.339876609353166	0.0
30	9.205509313213772E-5	0.0	0.0	15.793800273587737	0.0
31	9.205509313213772E-5	0.0	0.0	16.240359530371737	0.0
32	9.205509313213772E-5	0.0	0.0	16.643468783197367	0.0
33	9.205509313213772E-5	0.0	0.0	17.01813301224517	0.0
34	9.205509313213772E-5	0.0	0.0	17.42032171413948	0.0
35	9.205509313213772E-5	0.0	0.0	17.84377514254731	0.0
36	9.205509313213772E-5	0.0	0.0	18.235653674010823	0.0
37	9.205509313213772E-5	0.0	0.0	18.63959142267464	0.0
38	9.205509313213772E-5	0.0	0.0	19.041872179662086	0.0
39	1.8411018626427543E-4	0.0	0.0	19.44691458944349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAATC	20	7.033116E-4	45.0	23
CCGATCC	20	7.033116E-4	45.0	42
ACGTTAC	20	7.033116E-4	45.0	38
CGCGACG	20	7.033116E-4	45.0	1
TAGCCCG	25	3.890664E-5	45.0	1
CAAATCG	25	3.890664E-5	45.0	18
TAGTACG	20	7.033116E-4	45.0	1
CCCGATC	20	7.033116E-4	45.0	41
GCCCGAT	30	2.1653923E-6	44.999996	39
CCCGATA	30	2.1653923E-6	44.999996	40
AGGTCGT	30	2.1653923E-6	44.999996	14
CGTTTTT	29695	0.0	44.621147	1
TTGCGAG	55	6.184564E-11	40.909092	1
ATCTACG	45	1.9281288E-8	40.000004	1
GTTTTTT	33355	0.0	39.839603	2
TTTAGCG	40	3.4579898E-7	39.375	1
TTAATCG	40	3.4579898E-7	39.375	20
TCTACGG	75	0.0	39.0	2
AATACGG	75	0.0	39.0	2
ACGGGTC	35	6.247974E-6	38.571426	5
>>END_MODULE