##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934932.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1391824
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.657236834542296	34.0	31.0	34.0	31.0	34.0
2	32.80892411684236	34.0	31.0	34.0	31.0	34.0
3	32.85421288898596	34.0	31.0	34.0	31.0	34.0
4	36.2347516640035	37.0	37.0	37.0	35.0	37.0
5	36.12976640724689	37.0	37.0	37.0	35.0	37.0
6	36.13103237190909	37.0	36.0	37.0	35.0	37.0
7	36.270671435468856	37.0	37.0	37.0	35.0	37.0
8	36.27310205888101	37.0	37.0	37.0	35.0	37.0
9	38.047482296612216	39.0	39.0	39.0	37.0	39.0
10	37.668504782213844	39.0	38.0	39.0	35.0	39.0
11	37.56748482566761	39.0	37.0	39.0	35.0	39.0
12	37.508246013863825	39.0	37.0	39.0	35.0	39.0
13	37.48541841497201	39.0	37.0	39.0	35.0	39.0
14	38.82560797916978	40.0	38.0	41.0	35.0	41.0
15	38.87051020818724	40.0	38.0	41.0	35.0	41.0
16	38.94768375886606	41.0	38.0	41.0	35.0	41.0
17	38.96518094241801	41.0	38.0	41.0	35.0	41.0
18	38.41276123992689	39.0	38.0	41.0	35.0	41.0
19	37.89082240283254	38.0	37.0	41.0	35.0	41.0
20	37.28725686581062	38.0	35.0	41.0	34.0	41.0
21	37.236754790835626	38.0	35.0	41.0	34.0	41.0
22	37.054657054340204	38.0	35.0	41.0	34.0	41.0
23	36.94248841807585	38.0	35.0	40.0	34.0	41.0
24	36.7342422605157	37.0	35.0	40.0	33.0	41.0
25	36.596342641023575	37.0	35.0	40.0	33.0	41.0
26	36.69956546229983	37.0	35.0	40.0	33.0	41.0
27	36.73768809849521	37.0	35.0	40.0	33.0	41.0
28	36.65933049006196	37.0	35.0	40.0	33.0	41.0
29	36.5264056374944	37.0	35.0	40.0	33.0	41.0
30	36.20481181528699	37.0	35.0	40.0	33.0	41.0
31	35.65633370311189	37.0	35.0	40.0	30.0	41.0
32	34.87544761406615	37.0	35.0	40.0	22.0	41.0
33	33.85548172757475	37.0	34.0	40.0	15.0	41.0
34	32.99862554460909	37.0	33.0	40.0	10.0	41.0
35	32.483317574635876	37.0	32.0	40.0	9.0	41.0
36	32.24475580245778	37.0	31.0	40.0	8.0	41.0
37	31.995834961891735	37.0	30.0	40.0	8.0	41.0
38	31.841231362586075	37.0	30.0	40.0	7.0	41.0
39	31.766233374334686	37.0	29.0	40.0	7.0	41.0
40	31.63690452241088	37.0	29.0	40.0	7.0	41.0
41	31.49093491705848	37.0	28.0	40.0	7.0	41.0
42	31.351982721953352	36.0	27.0	40.0	7.0	41.0
43	31.23219458782145	36.0	25.0	40.0	7.0	41.0
44	31.121618106887077	36.0	24.0	40.0	7.0	41.0
45	31.09507164698985	36.0	24.0	40.0	7.0	41.0
46	31.05515424364	36.0	24.0	40.0	7.0	41.0
47	31.0466797526124	35.0	24.0	40.0	7.0	41.0
48	30.998416466449783	35.0	24.0	40.0	7.0	41.0
49	30.87732572509168	35.0	24.0	40.0	7.0	41.0
50	30.779160296129394	35.0	24.0	40.0	7.0	41.0
51	30.239801871500994	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	11.0
11	9.0
12	20.0
13	20.0
14	39.0
15	73.0
16	107.0
17	258.0
18	480.0
19	944.0
20	1623.0
21	2653.0
22	4031.0
23	7171.0
24	13934.0
25	30344.0
26	56899.0
27	74820.0
28	71061.0
29	55484.0
30	42504.0
31	35824.0
32	34270.0
33	39802.0
34	68143.0
35	93211.0
36	95496.0
37	119772.0
38	224124.0
39	318435.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.446412764832335	15.252503190058514	17.6888026072262	38.61228143788295
2	51.70222671832071	17.781774132361562	17.555667958017683	12.960331191300051
3	22.95318948372783	17.474048442906575	46.7924105346653	12.780351538700296
4	21.14326236650611	18.580366483118553	46.610059892630105	13.666311257745232
5	18.5409218406925	21.8081452827369	45.40186115485866	14.249071721711939
6	19.838643391693203	24.28079987124809	44.76507087102967	11.115485866029038
7	58.42304774166849	4.424553679200819	33.604823598386005	3.5475749807446917
8	58.89250364988676	4.195573578268517	33.38561484785433	3.5263079239903896
9	54.12185736127556	5.9753963144765425	35.04106841094851	4.861677913299383
10	29.66603536079274	20.266499212544115	39.802661830806194	10.264803595856947
11	22.967630964834633	18.595598294037178	44.78856522089	13.648205520238191
12	20.912198668797206	16.81117727528768	47.841968524756	14.434655531159112
13	17.458744783823242	18.46188885951097	48.9312585499316	15.148107806734185
14	14.624693926818333	20.97147340468335	48.295330444079134	16.10850222441918
15	13.651654232144296	18.811645725321593	52.47387600731127	15.062824035222844
16	15.131367184356643	18.412098081366608	50.995887411051974	15.460647323224775
17	15.64350090241295	18.2852142224879	47.65415742220281	18.41712745289634
18	15.839646392072563	19.395483911758955	48.86760107599811	15.897268620170365
19	17.574635873501247	19.300931726999966	47.136347699134376	15.988084700364414
20	19.11024669785835	19.275210084033613	47.16214119026544	14.4524020278426
21	17.601507087102966	18.915681867822368	47.57282529975054	15.909985745324121
22	16.51264815091563	19.437730632608723	46.38460035176861	17.66502086470703
23	16.35673763349389	19.375222729310604	47.420435342399614	16.847604294795893
24	16.69463955212728	18.23880030808493	48.42638149651105	16.640178643276737
25	16.4102645162032	19.39735196404143	46.9521290048167	17.240254514938673
26	15.169087470829645	20.714544367678673	47.30195771879203	16.81441044269965
27	16.27914161560657	20.383036935704517	47.84908149306234	15.488739955626574
28	14.51505362747014	20.6996717975836	48.440822977617856	16.344451597328398
29	15.081863798871122	19.18827380473393	48.3224890503397	17.407373346055248
30	15.789999310257619	20.210098403246388	47.06428398992976	16.935618296566233
31	16.898832036234467	21.795428157583142	44.28922047615216	17.016519330030235
32	16.932241432824842	22.000339123337433	43.77141075308372	17.296008690754004
33	17.940271183712884	23.550319580636632	41.37426858568325	17.135140649967237
34	17.63247364609318	24.61036740277506	39.55672556300222	18.200433388129532
35	17.576216533124878	24.04003667130327	37.72876455643817	20.654982239133684
36	18.813944866592326	26.969573739208407	36.27592281782754	17.940558576371725
37	18.50341709871363	27.230813666095717	37.17934164089712	17.086427594293532
38	18.1638626722919	26.89564197772132	35.86372989688351	19.076765453103267
39	19.333263401119684	26.200079895159156	35.935793606088126	18.530863097633034
40	19.9627251721482	25.218705813378705	36.25192553081424	18.566643483658854
41	17.75008909172424	26.346003517686146	35.21903631493637	20.684871075653245
42	18.819117934451484	26.426401613997175	34.66832013243054	20.086160319120808
43	19.495712101530078	26.11493981997724	35.1239093448597	19.26543873363299
44	18.09036199979308	27.005354125234227	34.77293106024899	20.1313528147237
45	18.00249169435216	25.77869040913219	34.95628757659014	21.262530319925506
46	18.284136500017244	26.22414903033717	35.5003218797779	19.991392589867687
47	17.874601961167507	25.22222637344952	37.43993493430204	19.46323673108094
48	17.280345790847118	23.836634517007898	37.75736012599294	21.12565956615204
49	17.65273482854154	23.774773247192176	38.447174355378266	20.125317568888022
50	17.003586660382346	23.7338197933072	38.28946763383876	20.97312591247169
51	16.83840772971295	23.409353481474668	36.589683753118216	23.16255503569417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	816.5
2	1278.0
3	59748.5
4	118219.0
5	82460.0
6	46701.0
7	44443.0
8	42185.0
9	40640.0
10	39095.0
11	36486.0
12	33877.0
13	31309.5
14	28742.0
15	26226.0
16	23710.0
17	21488.0
18	19266.0
19	17896.0
20	16526.0
21	15143.0
22	13760.0
23	13024.0
24	12288.0
25	12422.0
26	13282.0
27	14008.0
28	15580.5
29	17153.0
30	19228.5
31	21304.0
32	21954.5
33	22605.0
34	24892.5
35	27180.0
36	29247.5
37	31315.0
38	35443.5
39	39572.0
40	42598.0
41	45624.0
42	51574.5
43	57525.0
44	65392.0
45	73259.0
46	79186.5
47	85114.0
48	88504.5
49	91895.0
50	90783.5
51	89672.0
52	80269.5
53	70867.0
54	62811.0
55	54755.0
56	49888.0
57	45021.0
58	44576.5
59	44132.0
60	43690.5
61	43249.0
62	38726.0
63	34203.0
64	30123.5
65	26044.0
66	21685.5
67	17327.0
68	14432.0
69	11537.0
70	9634.0
71	7731.0
72	6375.0
73	5019.0
74	4095.0
75	2425.5
76	1680.0
77	1544.0
78	1408.0
79	1021.0
80	634.0
81	390.0
82	146.0
83	107.5
84	69.0
85	46.0
86	23.0
87	16.0
88	9.0
89	10.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1391824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.725890452699762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13118254980684	24.787813385150592
2	9.552891478094413	6.061479770811049
3	3.565305131753539	3.393374401213832
4	1.7053132116674599	2.1641032056361373
5	0.995704755810882	1.5794810003044102
6	0.6315424688991659	1.202174831071349
7	0.4447065991811699	0.987609899445012
8	0.3357668963313009	0.8522003016519881
9	0.26175740635407196	0.7474038119254893
>10	3.3336065233136156	27.17036194516061
>50	0.9390211827201168	19.39319518652129
>100	0.09719900706008677	5.404283076216355
>500	0.00323227100395669	0.6857442704991921
>1k	0.0025396415031088285	1.2646935029308963
>5k	0.0	0.0
>10k+	2.3087650028262075E-4	4.306081411461921
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58788	4.223809906999736	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3067	0.2203583211670441	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3016	0.21669406476680958	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	1690	0.12142339836071227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1550	0.111364655301245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.184816471048064E-5	0.0	0.0	0.12975778546712802	0.0
2	7.184816471048064E-5	0.0	0.0	0.7628119647311729	0.0
3	7.184816471048064E-5	0.0	0.0	1.1050966214119027	0.0
4	7.184816471048064E-5	0.0	0.0	1.4440762625159502	0.0
5	7.184816471048064E-5	0.0	0.0	2.353171090597662	0.0
6	7.184816471048064E-5	0.0	0.0	2.828734092816333	0.0
7	7.184816471048064E-5	0.0	0.0	3.319744450447758	0.0
8	7.184816471048064E-5	0.0	0.0	4.039806757176195	0.0
9	7.184816471048064E-5	0.0	0.0	4.309309223005208	0.0
10	7.184816471048064E-5	0.0	0.0	4.909025853843589	0.0
11	7.184816471048064E-5	0.0	0.0	5.84046546115026	0.0
12	7.184816471048064E-5	0.0	0.0	6.563114301808275	0.0
13	7.184816471048064E-5	0.0	0.0	6.843034751520308	0.0
14	7.184816471048064E-5	0.0	0.0	6.946783501362241	0.0
15	7.184816471048064E-5	0.0	0.0	7.17827828805941	0.0
16	7.184816471048064E-5	0.0	0.0	7.652476175148582	0.0
17	7.184816471048064E-5	0.0	0.0	8.209012058995965	0.0
18	7.184816471048064E-5	0.0	0.0	8.859525342284657	0.0
19	7.184816471048064E-5	0.0	0.0	9.22774718642587	0.0
20	1.4369632942096128E-4	0.0	0.0	9.60904539654439	0.0
21	1.4369632942096128E-4	0.0	0.0	10.064634608973549	0.0
22	1.4369632942096128E-4	0.0	0.0	10.546448401522031	0.0
23	1.4369632942096128E-4	0.0	0.0	11.022226948234835	0.0
24	1.4369632942096128E-4	0.0	0.0	11.38951476623481	0.0
25	2.155444941314419E-4	0.0	0.0	11.727775925691754	0.0
26	2.155444941314419E-4	0.0	0.0	12.059570750324754	0.0
27	2.155444941314419E-4	0.0	0.0	12.36528469116785	0.0
28	2.155444941314419E-4	0.0	0.0	12.692193840600536	0.0
29	2.155444941314419E-4	0.0	0.0	13.025497476692456	0.0
30	2.155444941314419E-4	0.0	0.0	13.400688592810585	0.0
31	2.155444941314419E-4	0.0	0.0	13.756049615468623	0.0
32	2.155444941314419E-4	0.0	0.0	14.104728758808585	0.0
33	2.155444941314419E-4	0.0	0.0	14.407856165722103	0.0
34	2.155444941314419E-4	0.0	0.0	14.733615744519422	0.0
35	2.155444941314419E-4	0.0	0.0	15.074966375058915	0.0
36	2.155444941314419E-4	0.0	0.0	15.430327397716953	0.0
37	2.155444941314419E-4	0.0	0.0	15.784826242398465	0.0
38	2.155444941314419E-4	0.0	0.0	16.118345422984515	0.0
39	2.155444941314419E-4	0.0	0.0	16.457396912253273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	40	6.8175723E-9	45.0	1
TAGCCGA	25	3.8912316E-5	45.0	45
TACGATC	20	7.0338004E-4	45.0	12
CCAACGA	20	7.0338004E-4	45.0	9
TCGAAGT	20	7.0338004E-4	45.0	11
TCGAACG	35	1.2121745E-7	45.0	1
TACGTCG	20	7.0338004E-4	45.0	24
CGTTTTT	39690	0.0	44.53515	1
TAGAACG	50	1.0822987E-9	40.5	1
GTTTTTT	43960	0.0	40.378185	2
ACGTTAG	80	0.0	39.375	1
CGGTCTA	40	3.4589175E-7	39.375	31
ACGGTCT	40	3.4589175E-7	39.375	30
TACGCGG	70	0.0	38.571426	2
GCGATAT	70	0.0	38.571426	9
TAATCGG	35	6.24924E-6	38.571426	1
AACGTCG	30	1.14004564E-4	37.499996	44
GATCGAT	30	1.14004564E-4	37.499996	9
ATATCGA	30	1.14004564E-4	37.499996	19
CGGATAC	30	1.14004564E-4	37.499996	14
>>END_MODULE