##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934931.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1495724
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59846736429983	34.0	31.0	34.0	31.0	34.0
2	32.76180765970192	34.0	31.0	34.0	31.0	34.0
3	32.80858233203452	34.0	31.0	34.0	31.0	34.0
4	36.20811058724738	37.0	37.0	37.0	35.0	37.0
5	36.09947624026893	37.0	35.0	37.0	35.0	37.0
6	36.09616613760293	37.0	36.0	37.0	35.0	37.0
7	36.253122902353645	37.0	37.0	37.0	35.0	37.0
8	36.27590986037531	37.0	37.0	37.0	35.0	37.0
9	38.07747886642188	39.0	39.0	39.0	37.0	39.0
10	37.519533015449376	39.0	37.0	39.0	34.0	39.0
11	37.470608882387396	39.0	37.0	39.0	34.0	39.0
12	37.46519678764264	39.0	37.0	39.0	35.0	39.0
13	37.46732953405842	39.0	37.0	39.0	35.0	39.0
14	38.789320088465516	40.0	38.0	41.0	35.0	41.0
15	38.81999419679032	40.0	38.0	41.0	35.0	41.0
16	38.90270196907986	40.0	38.0	41.0	35.0	41.0
17	38.909510043296756	40.0	38.0	41.0	35.0	41.0
18	38.349317788575966	39.0	38.0	40.0	35.0	41.0
19	37.772262797147064	38.0	37.0	40.0	35.0	41.0
20	37.17737831311124	38.0	35.0	40.0	34.0	41.0
21	37.13108568158297	38.0	35.0	40.0	34.0	41.0
22	36.97475269501592	38.0	35.0	40.0	34.0	41.0
23	36.858778758647986	37.0	35.0	40.0	33.0	41.0
24	36.641295452904416	37.0	35.0	40.0	33.0	41.0
25	36.50100954454164	37.0	35.0	40.0	33.0	41.0
26	36.62971778215767	37.0	35.0	40.0	33.0	41.0
27	36.65998205551291	37.0	35.0	40.0	33.0	41.0
28	36.58691041930196	37.0	35.0	40.0	33.0	41.0
29	36.43119653091078	37.0	35.0	40.0	33.0	41.0
30	36.10510361537289	37.0	35.0	40.0	32.0	41.0
31	35.51505424797623	37.0	35.0	40.0	30.0	41.0
32	34.65149586421024	37.0	35.0	40.0	21.0	41.0
33	33.50440589306583	37.0	33.0	40.0	15.0	41.0
34	32.522365757318866	37.0	32.0	40.0	10.0	41.0
35	31.921213405681797	37.0	30.0	40.0	7.0	41.0
36	31.644120171903374	37.0	29.0	40.0	7.0	41.0
37	31.426366094279427	37.0	27.0	40.0	7.0	41.0
38	31.294504868545268	37.0	25.0	40.0	7.0	41.0
39	31.22705726457555	37.0	25.0	40.0	7.0	41.0
40	31.136112678542297	37.0	24.0	40.0	7.0	41.0
41	31.021092126622293	37.0	23.0	40.0	7.0	41.0
42	30.90766411450241	37.0	23.0	40.0	7.0	41.0
43	30.79741783912005	36.0	23.0	40.0	7.0	41.0
44	30.67815318868989	36.0	23.0	40.0	7.0	41.0
45	30.63355204569827	36.0	23.0	40.0	7.0	41.0
46	30.558749475170554	36.0	23.0	40.0	7.0	41.0
47	30.514395035447716	36.0	23.0	40.0	7.0	41.0
48	30.431151736550326	35.0	23.0	40.0	7.0	41.0
49	30.29736569046161	35.0	22.0	40.0	7.0	41.0
50	30.202318074725017	35.0	22.0	40.0	7.0	41.0
51	29.641376350182252	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	7.0
11	13.0
12	10.0
13	22.0
14	32.0
15	66.0
16	130.0
17	270.0
18	539.0
19	1054.0
20	1764.0
21	2853.0
22	4851.0
23	8926.0
24	19062.0
25	42031.0
26	74375.0
27	86260.0
28	74247.0
29	56243.0
30	43815.0
31	38149.0
32	36668.0
33	42529.0
34	70817.0
35	95584.0
36	94639.0
37	135095.0
38	249778.0
39	315690.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.330360414087092	15.873115628284362	18.370434652382393	37.42608930524616
2	52.79550237878111	16.96522887912476	18.189385207431318	12.049883534662813
3	21.683345323067623	17.527297816976926	48.288186858003215	12.501170001952232
4	19.88080688683206	18.50301258788386	48.72630244617323	12.889878079110852
5	17.83858519352501	21.428886612770807	47.18042900963012	13.552099184074068
6	18.704787781703043	24.274398217853026	46.717241951055136	10.30357204938879
7	57.39708662828169	4.279064854211071	34.864854745929065	3.458993771578179
8	58.68850135452798	3.3576381738876964	34.49419812746201	3.4596623441223113
9	54.63574830650575	5.08877306240991	35.827465494970994	4.448013136113347
10	28.85766357964437	16.4622617541739	43.01943406671284	11.660640599468886
11	22.22983652064151	18.446050207123772	45.13780617279659	14.18630709943813
12	19.428116417199963	16.716185606435413	49.07763731811484	14.778060658249784
13	17.298512292374795	18.659324848702035	50.41023611307969	13.631926745843485
14	15.532143630776801	20.34459566069676	49.282153659364965	14.841107049161476
15	14.76930235792165	19.57974867020921	52.20488539329449	13.446063578574657
16	16.857388127756188	18.228830987535133	50.50430427003912	14.40947661466955
17	17.283001409350923	18.101735346895552	48.13174088267622	16.48352236107731
18	17.515731511963438	18.933640163559588	49.09622363484172	14.454404689635252
19	16.916222511639848	20.665711053643587	47.777130005268354	14.640936429448214
20	17.780218810422245	19.82645193899409	48.067156774913016	14.326172475670646
21	16.739719359988875	20.147433617432092	48.91303475774942	14.199812264829609
22	16.019533015449376	18.99895970112133	47.8586289983981	17.122878285031195
23	15.533480775865065	19.585297822325508	48.35785211710182	16.523369284707606
24	16.245777964383805	18.463499950525634	49.356565783526904	15.934156301563657
25	15.270196907985698	19.831131946803023	48.09737625390781	16.801294891303474
26	14.67710620408578	20.61864354653666	49.024418943601894	15.679831305775666
27	15.227943123196525	20.591365786736056	48.82424832388863	15.356442766178787
28	14.059946888597095	20.794210696625846	49.44120706761408	15.704635347162979
29	14.643878148642397	20.03765400568554	48.4041841944102	16.91428365126186
30	15.167370450698122	20.363649978204535	47.829144949201854	16.63983462189548
31	15.949800899096358	21.6095349141954	45.47597016561879	16.964694021089453
32	16.048549063864723	22.2671428686041	43.89867381950146	17.785634248029716
33	16.35836558081571	23.167710085550546	41.93454139934908	18.539382934284667
34	16.042197624695465	25.1766368661598	39.916054031358726	18.86511147778601
35	16.708029021396996	25.164535703111003	37.7119709251172	20.4154643503748
36	17.508644642995634	27.77611377500127	36.296469134679924	18.41877244732317
37	17.431691943165987	27.718148535424987	36.691796080025455	18.15836344138357
38	17.19916241231671	27.138696711425368	35.04363104423008	20.618509832027833
39	18.509230312544293	26.214996884451946	35.2617194081261	20.014053394877664
40	18.765360454201442	25.200638620494153	36.18943067036432	19.844570254940084
41	17.068255908175573	26.546475151832826	35.427859685343016	20.957409254648585
42	17.242953913957386	27.12164811154999	35.41676138111042	20.218636593382204
43	17.38876958583268	26.28860672156093	36.133404291166016	20.189219401440372
44	16.967903169301287	25.999114809951568	35.362005289745966	21.670976731001172
45	17.968221409832292	24.8894849584549	34.87842676857495	22.263866863137853
46	17.87361839483755	25.349061725291566	35.732461336449774	21.044858543421114
47	16.588956251287	25.321850822745372	37.40275612345593	20.686436802511693
48	16.907330496802885	24.116481382928935	37.4888682671402	21.487319853127985
49	17.05876217804889	23.815690595323737	37.72340351562187	21.402143711005507
50	16.546635609243417	23.340402373699963	37.78344133008496	22.32952068697166
51	16.306551208645445	23.638786300146283	35.86136212295851	24.193300368249755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	710.0
2	1079.0
3	57673.0
4	114267.0
5	80344.0
6	46421.0
7	44328.0
8	42235.0
9	41685.0
10	41135.0
11	40015.5
12	38896.0
13	37364.0
14	35832.0
15	33519.0
16	31206.0
17	28811.0
18	26416.0
19	24284.0
20	22152.0
21	20201.0
22	18250.0
23	16861.5
24	15473.0
25	14907.5
26	14254.5
27	14167.0
28	15755.5
29	17344.0
30	18225.5
31	19107.0
32	21789.0
33	24471.0
34	25699.0
35	26927.0
36	30661.5
37	34396.0
38	37845.5
39	41295.0
40	46897.5
41	52500.0
42	59239.5
43	65979.0
44	74710.0
45	83441.0
46	93199.5
47	102958.0
48	110417.5
49	117877.0
50	112640.0
51	107403.0
52	93951.5
53	80500.0
54	69903.0
55	59306.0
56	54319.5
57	49333.0
58	44883.0
59	40433.0
60	38134.5
61	35836.0
62	31284.5
63	26733.0
64	22728.0
65	18723.0
66	15130.0
67	11537.0
68	9369.5
69	7202.0
70	5632.0
71	4062.0
72	3346.0
73	2630.0
74	2257.5
75	1414.0
76	943.0
77	674.0
78	405.0
79	252.5
80	100.0
81	113.0
82	126.0
83	76.0
84	26.0
85	21.0
86	16.0
87	14.5
88	13.0
89	7.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1495724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.16992343993019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93723872831453	32.110727597158174
2	9.012754225101066	7.240832944104343
3	3.191321305836997	3.845853975830707
4	1.5689618069359326	2.521003026591638
5	0.9872289988880786	1.982845665150652
6	0.6625975257004335	1.5969895127324152
7	0.5031355082614596	1.4147640392741236
8	0.3693002639769591	1.1867810664240364
9	0.30565144606886097	1.1050195669101088
>10	3.0133241702149487	26.898578627526078
>50	0.3824048453136333	9.670796744031197
>100	0.060815069452958684	4.478829769199182
>500	0.002548115774500183	0.6544745940041384
>1k	0.002548115774500183	1.4557982471571564
>5k	0.0	0.0
>10k+	1.6987438496667888E-4	3.8367046239060247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56225	3.759049129384833	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	2860	0.19121174762188745	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	2204	0.1473533887267972	No Hit
CGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	1728	0.11552933562609144	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1723	0.11519504935402522	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1594	0.10657046353471629	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.685725441324736E-5	0.0	0.0	0.12321791988361488	0.0
2	6.685725441324736E-5	0.0	0.0	0.6610176743837767	0.0
3	6.685725441324736E-5	0.0	0.0	1.0447114574614034	0.0
4	6.685725441324736E-5	0.0	0.0	1.4409744043687205	0.0
5	6.685725441324736E-5	0.0	0.0	2.435008062984882	0.0
6	6.685725441324736E-5	0.0	0.0	3.10284517731881	0.0
7	6.685725441324736E-5	0.0	0.0	3.7131850528573453	0.0
8	6.685725441324736E-5	0.0	0.0	4.591823090356242	0.0
9	6.685725441324736E-5	0.0	0.0	4.912604197031003	0.0
10	6.685725441324736E-5	0.0	0.0	5.687412918426126	0.0
11	6.685725441324736E-5	0.0	0.0	6.756660988257192	0.0
12	6.685725441324736E-5	0.0	0.0	7.539826866453971	0.0
13	6.685725441324736E-5	0.0	0.0	7.862747405269956	0.0
14	6.685725441324736E-5	0.0	0.0	7.985229895355025	0.0
15	6.685725441324736E-5	0.0	0.0	8.223174863811773	0.0
16	6.685725441324736E-5	0.0	0.0	8.779761506802057	0.0
17	6.685725441324736E-5	0.0	0.0	9.501819854465129	0.0
18	6.685725441324736E-5	0.0	0.0	10.274556000973442	0.0
19	6.685725441324736E-5	0.0	0.0	10.744295070480918	0.0
20	6.685725441324736E-5	0.0	0.0	11.196517539332122	0.0
21	1.3371450882649472E-4	0.0	0.0	11.781451658193625	0.0
22	1.3371450882649472E-4	0.0	0.0	12.386710382396751	0.0
23	2.0057176323974208E-4	0.0	0.0	13.075808103634094	0.0
24	2.0057176323974208E-4	0.0	0.0	13.571354073345082	0.0
25	2.0057176323974208E-4	0.0	0.0	14.050787444742479	0.0
26	2.0057176323974208E-4	0.0	0.0	14.501137910470113	0.0
27	2.0057176323974208E-4	0.0	0.0	14.940657500982802	0.0
28	2.0057176323974208E-4	0.0	0.0	15.408925710893186	0.0
29	2.0057176323974208E-4	0.0	0.0	15.865761330298906	0.0
30	2.0057176323974208E-4	0.0	0.0	16.362377016080508	0.0
31	2.6742901765298944E-4	0.0	0.0	16.8761081589919	0.0
32	2.6742901765298944E-4	0.0	0.0	17.37887471217952	0.0
33	2.6742901765298944E-4	0.0	0.0	17.857505796523956	0.0
34	2.6742901765298944E-4	0.0	0.0	18.304446542276516	0.0
35	2.6742901765298944E-4	0.0	0.0	18.75499757976739	0.0
36	3.342862720662368E-4	0.0	0.0	19.209961196049537	0.0
37	3.342862720662368E-4	0.0	0.0	19.691065998807268	0.0
38	3.342862720662368E-4	0.0	0.0	20.183469677560833	0.0
39	3.342862720662368E-4	0.0	0.0	20.659693900746394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	30	2.1659416E-6	45.000004	1
AAGCGCG	35	1.2122655E-7	45.000004	1
ACTACGG	30	2.1659416E-6	45.000004	2
AGCGTGC	20	7.0339703E-4	45.0	12
CCTACGC	20	7.0339703E-4	45.0	28
CACTACG	20	7.0339703E-4	45.0	1
TCGTAGC	20	7.0339703E-4	45.0	18
ACGGTAC	20	7.0339703E-4	45.0	15
TCGTAAG	65	0.0	44.999996	1
CGTTTTT	44420	0.0	44.554256	1
ACCGGGT	90	0.0	40.0	4
CCGACGT	45	1.9290383E-8	40.0	12
GTTTTTT	50440	0.0	39.39284	2
ACGCGAG	40	3.459154E-7	39.375	1
CGATCAT	35	6.249551E-6	38.57143	10
ACCTCGT	30	1.1400861E-4	37.500004	38
TAGATCG	30	1.1400861E-4	37.500004	1
CGGTAAT	30	1.1400861E-4	37.500004	15
TCGACGG	60	1.5643309E-10	37.500004	2
ATCGACG	30	1.1400861E-4	37.500004	1
>>END_MODULE