##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920708
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.374512874874554	33.0	31.0	34.0	30.0	34.0
2	32.56128327330706	34.0	31.0	34.0	31.0	34.0
3	32.63755935649522	34.0	31.0	34.0	31.0	34.0
4	36.06924562401978	37.0	35.0	37.0	35.0	37.0
5	35.934813208965274	37.0	35.0	37.0	35.0	37.0
6	35.93427449310748	37.0	35.0	37.0	35.0	37.0
7	36.15680867332531	37.0	36.0	37.0	35.0	37.0
8	36.1814831629572	37.0	37.0	37.0	35.0	37.0
9	37.993257362812095	39.0	39.0	39.0	35.0	39.0
10	37.48565994864821	39.0	37.0	39.0	35.0	39.0
11	37.331469912284895	39.0	37.0	39.0	34.0	39.0
12	37.37390247505181	39.0	37.0	39.0	34.0	39.0
13	37.359104080772624	39.0	37.0	39.0	34.0	39.0
14	38.575035733370406	40.0	38.0	41.0	34.0	41.0
15	38.61896714267716	40.0	38.0	41.0	34.0	41.0
16	38.699638756261486	40.0	38.0	41.0	35.0	41.0
17	38.75004127258588	40.0	38.0	41.0	35.0	41.0
18	38.301642866142146	39.0	38.0	40.0	35.0	41.0
19	37.85902479396291	38.0	37.0	40.0	35.0	41.0
20	37.30512496904556	38.0	35.0	40.0	34.0	41.0
21	37.26318333282648	38.0	35.0	40.0	34.0	41.0
22	37.07333161002185	38.0	35.0	40.0	34.0	41.0
23	36.9155856145488	38.0	35.0	40.0	33.0	41.0
24	36.65833032839945	38.0	35.0	40.0	33.0	41.0
25	36.502175499724125	37.0	35.0	40.0	33.0	41.0
26	36.68673998705344	38.0	35.0	40.0	33.0	41.0
27	36.71820273094184	38.0	35.0	40.0	33.0	41.0
28	36.70624997284698	38.0	35.0	40.0	33.0	41.0
29	36.6075987175087	38.0	35.0	40.0	33.0	41.0
30	36.2878958366822	38.0	35.0	40.0	32.0	41.0
31	35.828592778600814	38.0	35.0	40.0	30.0	41.0
32	35.11832198699262	38.0	35.0	40.0	24.0	41.0
33	34.157187729442995	38.0	34.0	40.0	18.0	41.0
34	33.42381297870769	38.0	33.0	40.0	12.0	41.0
35	32.93643044265934	38.0	33.0	40.0	10.0	41.0
36	32.76229054162666	38.0	33.0	40.0	8.0	41.0
37	32.577858561020435	38.0	32.0	40.0	8.0	41.0
38	32.43853100005648	38.0	31.0	40.0	8.0	41.0
39	32.35635510932891	38.0	31.0	40.0	8.0	41.0
40	32.24850767018425	38.0	31.0	40.0	8.0	41.0
41	32.164399570765106	38.0	31.0	40.0	8.0	41.0
42	32.07826259791378	38.0	30.0	40.0	7.0	41.0
43	31.98257536591406	38.0	30.0	40.0	7.0	41.0
44	31.861097112222332	38.0	30.0	40.0	7.0	41.0
45	31.793051651555107	38.0	29.0	40.0	7.0	41.0
46	31.707264409563077	37.0	29.0	40.0	7.0	41.0
47	31.6545234754124	37.0	29.0	40.0	7.0	41.0
48	31.599078100765933	37.0	29.0	40.0	7.0	41.0
49	31.522371913788085	37.0	28.0	40.0	7.0	41.0
50	31.431411478992253	37.0	27.0	40.0	7.0	41.0
51	30.87232868618498	36.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	9.0
11	7.0
12	2.0
13	4.0
14	10.0
15	20.0
16	66.0
17	151.0
18	297.0
19	555.0
20	1004.0
21	1623.0
22	2807.0
23	5393.0
24	11012.0
25	24088.0
26	39545.0
27	44396.0
28	36958.0
29	27786.0
30	22481.0
31	20693.0
32	21812.0
33	26905.0
34	43383.0
35	62344.0
36	68285.0
37	94850.0
38	176061.0
39	188077.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.980849520151885	16.713116427792524	20.52941866476668	33.77661538728891
2	47.56013850210925	18.682904894928683	19.512158034903575	14.244798568058496
3	23.552744192512716	17.85245702220465	45.91184175656126	12.682957028721376
4	21.32728291705948	19.98603248804181	45.13754632304705	13.549138271851662
5	18.53769055987349	22.155667160489536	44.22335854581475	15.083283733822233
6	19.414515785677978	24.01304213713794	43.451126741594514	13.121315335589568
7	60.20236600529159	3.9158995034256248	31.491417474378412	4.390317016904382
8	60.246136668737535	3.012681545071836	31.134083770315886	5.607098015874739
9	55.96291115098381	5.393132241709641	33.266573115472006	5.377383491834545
10	26.39566507513783	25.877259674076907	38.17138549898556	9.555689751799703
11	18.479691715505894	19.02492429738853	47.3925500810246	15.102833906080972
12	16.865281935206387	17.905242487303248	50.27413685989478	14.95533871759559
13	15.77644595246267	20.49238194954318	50.792107812683284	12.93906428531087
14	13.357872419920321	23.794188819908157	47.17901875513192	15.668920005039599
15	11.878358828206121	21.83417543890137	51.618645650955564	14.668820081936945
16	13.52611251341359	21.596858070093887	48.12459542004631	16.752433996446214
17	13.725958718725154	21.03381310904217	45.703632422005676	19.536595750226997
18	14.407933894350869	20.586983060861858	48.81134952666861	16.193733518118666
19	14.980102269123327	21.137754858217804	46.81245302528054	17.06968984737832
20	15.400430972686237	21.935293274306293	47.02240015292579	15.641875600081676
21	15.642418660422198	21.73381788797317	47.4532642270948	15.170499224509834
22	14.311160541670107	20.72524622355839	45.43362282069886	19.529970414072647
23	12.692514890714538	21.72730116388692	46.38767122692536	19.192512718473175
24	15.448546118856358	19.780647067256936	47.082462626587365	17.68834418729934
25	13.089383387566958	22.23636592709089	45.379534010783004	19.294716674559144
26	12.899420880452869	22.42100644286788	45.38496461418821	19.29460806249104
27	14.318980610573604	23.083540058302958	45.889359058463704	16.708120272659734
28	11.715223501913744	21.32815181360431	48.367452004327106	18.589172680154835
29	12.191052972277856	21.30295381380416	46.09170334134166	20.414289872576322
30	14.229918714728232	24.22809403198408	43.99603348727284	17.54595376601485
31	13.712273598144037	23.92159077579428	42.288108716335685	20.078026909725992
32	13.726501779065675	25.256867541066224	39.74321934858826	21.27341133127984
33	13.890288777766674	26.080038405227278	38.84271669193707	21.186956125068967
34	13.506453729086745	23.116015066666087	39.184084421988295	24.19344678225887
35	13.197995455671071	25.32735677326579	35.0893008423952	26.385346928667936
36	15.695095513452692	26.193212180191765	35.00273702411623	23.10895528223932
37	14.9212345282109	26.006399423052695	37.8975744752951	21.17479157344131
38	14.71812996085621	28.380659231808565	34.32793024498538	22.573280562349844
39	16.54943804115963	26.66143880578859	34.695039035177274	22.094084117874505
40	17.61731189475925	24.332796065636444	34.72154037979468	23.328351659809623
41	15.809681245302528	24.241996376701408	31.976804806735686	27.971517571260378
42	16.66326348853274	23.95862749101778	33.46793988973703	25.91016913071245
43	18.187525252305832	24.610517123778656	33.92888950677088	23.273068117144632
44	16.034291002141828	25.890401734317503	34.078013876277815	23.997293387262847
45	16.925778857140376	25.100031714723887	32.690820542452116	25.283368885683625
46	18.514230353163	24.48116015066666	33.79822918884163	23.206380307328708
47	14.600068642827042	24.31096503994752	36.5316691068178	24.55729721040764
48	15.189289112291846	22.882064671969832	35.28089253053085	26.64775368520747
49	16.57354992027874	21.605329811406005	37.9056117683348	23.91550849998045
50	16.220452086872278	21.615756569944	36.636479752538264	25.527311590645457
51	15.45202170503569	21.86806240414985	33.28080129639365	29.399114594420816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	475.5
2	553.0
3	25996.0
4	51439.0
5	35474.5
6	19510.0
7	19521.5
8	19533.0
9	20356.5
10	21180.0
11	21104.5
12	21029.0
13	20573.0
14	20117.0
15	19073.5
16	18030.0
17	16766.5
18	15503.0
19	14352.5
20	13202.0
21	12192.0
22	11182.0
23	10402.5
24	9623.0
25	9043.0
26	8778.5
27	9094.0
28	9436.0
29	9778.0
30	10864.0
31	11950.0
32	13159.0
33	14368.0
34	16737.5
35	19107.0
36	20647.0
37	22187.0
38	25480.0
39	28773.0
40	32479.0
41	36185.0
42	42117.0
43	48049.0
44	56462.0
45	64875.0
46	74554.5
47	84234.0
48	94365.5
49	104497.0
50	98357.5
51	92218.0
52	73422.0
53	54626.0
54	43700.5
55	32775.0
56	26552.0
57	20329.0
58	16920.5
59	13512.0
60	11696.5
61	9881.0
62	7943.0
63	6005.0
64	4806.0
65	3607.0
66	2884.0
67	2161.0
68	1513.5
69	866.0
70	851.5
71	837.0
72	586.0
73	335.0
74	318.0
75	254.5
76	208.0
77	156.5
78	105.0
79	88.0
80	71.0
81	40.0
82	9.0
83	5.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	920708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.37624419319867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72133587259974	29.059004760821022
2	10.648378094617872	8.17289516041126
3	4.40352703847252	5.069724868193232
4	2.181384993193555	3.3485345271269957
5	1.320349933946473	2.5335035742801795
6	0.8414382536032957	1.9374743936267222
7	0.5865685279945166	1.5757207946453866
8	0.4253932009096485	1.3059994684988074
9	0.3374113089451396	1.1653720907062928
>10	3.159942371342275	27.424293882082107
>50	0.31011812905280184	7.524781524053475
>100	0.05811102966409555	4.1138408534613005
>500	0.0034521403760579823	0.8773500056976601
>1k	0.0020137485527004896	1.7088839484326261
>5k	2.876783646714985E-4	0.634028071312654
>10k+	2.876783646714985E-4	3.5485920766502943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32143	3.4911177050704456	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	5743	0.6237591071219105	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	4746	0.5154728752221117	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	2710	0.2943387045621413	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	2561	0.2781555064146288	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	1582	0.17182429174070388	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	1503	0.1632439383604791	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC	1351	0.14673490400865422	No Hit
GCCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	1026	0.11143598187481807	TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	953	0.10350730090321796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.516724086246671E-4	0.0	0.0	0.3251845319037089	0.0
2	6.516724086246671E-4	0.0	0.0	2.0803555524661457	0.0
3	6.516724086246671E-4	0.0	0.0	2.9741242608948766	0.0
4	6.516724086246671E-4	0.0	0.0	3.908405270726441	0.0
5	6.516724086246671E-4	0.0	0.0	6.140166046129718	0.0
6	6.516724086246671E-4	0.0	0.0	7.228350356464808	0.0
7	6.516724086246671E-4	0.0	0.0	8.43915769168944	0.0
8	6.516724086246671E-4	0.0	0.0	10.244507487715975	0.0
9	6.516724086246671E-4	0.0	0.0	10.872285241357737	0.0
10	6.516724086246671E-4	0.0	0.0	12.223527980640984	0.0
11	7.602844767287783E-4	0.0	0.0	14.375784722192051	0.0
12	7.602844767287783E-4	0.0	0.0	16.112165854972478	0.0
13	7.602844767287783E-4	0.0	0.0	16.748523961994465	0.0
14	7.602844767287783E-4	0.0	0.0	16.973676779174287	0.0
15	7.602844767287783E-4	0.0	0.0	17.382818439722474	0.0
16	7.602844767287783E-4	0.0	0.0	18.33426015631449	0.0
17	7.602844767287783E-4	0.0	0.0	19.738831421036853	0.0
18	7.602844767287783E-4	0.0	0.0	21.20704935766823	0.0
19	7.602844767287783E-4	0.0	0.0	22.063238290532937	0.0
20	7.602844767287783E-4	0.0	0.0	22.806905120841787	0.0
21	7.602844767287783E-4	0.0	0.0	23.727066561819818	0.0
22	7.602844767287783E-4	0.0	0.0	24.739656872754445	0.0
23	7.602844767287783E-4	0.0	0.0	25.724225270118215	0.0
24	7.602844767287783E-4	0.0	0.0	26.460832316000296	0.0
25	7.602844767287783E-4	0.0	0.0	27.080898612806667	0.0
26	7.602844767287783E-4	0.0	0.0	27.670553530543884	0.0
27	7.602844767287783E-4	0.0	0.0	28.23490183641285	0.0
28	7.602844767287783E-4	0.0	0.0	28.840305504025164	0.0
29	7.602844767287783E-4	0.0	0.0	29.478510016204922	0.0
30	8.688965448328895E-4	0.0	0.0	30.165807183167736	0.0
31	9.775086129370006E-4	0.0	0.0	30.82301880726571	0.0
32	9.775086129370006E-4	0.0	0.0	31.411044543981372	0.0
33	9.775086129370006E-4	0.0	0.0	31.957254634476946	0.0
34	0.0010861206810411118	0.0	0.0	32.4897796043914	0.0
35	0.0010861206810411118	0.0	0.0	33.08562541001056	0.0
36	0.0010861206810411118	0.0	0.0	33.6283599143268	0.0
37	0.0010861206810411118	0.0	0.0	34.16251406526282	0.0
38	0.001194732749145223	0.0	0.0	34.67755249221251	0.0
39	0.0013033448172493343	0.0	0.0	35.19997653979329	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAGG	95	0.0	45.000004	2
TGCGTAG	85	0.0	45.000004	1
TATCACG	20	7.032555E-4	45.0	1
TTAGGCG	25	3.890199E-5	45.0	1
TAATACG	25	3.890199E-5	45.0	1
TCGTTAC	20	7.032555E-4	45.0	37
AGTCCCG	20	7.032555E-4	45.0	14
CGAACCG	25	3.890199E-5	45.0	2
CGAGTTA	20	7.032555E-4	45.0	13
GCGCGGC	20	7.032555E-4	45.0	3
TAGCGCG	45	3.8380676E-10	45.0	1
CCGTGAA	20	7.032555E-4	45.0	22
GTTAGCG	50	2.1827873E-11	45.0	1
CACTGCG	25	3.890199E-5	45.0	1
TAACGCG	40	6.8121153E-9	45.0	1
CGTTACC	20	7.032555E-4	45.0	38
TCGCTAT	20	7.032555E-4	45.0	14
CCATACG	20	7.032555E-4	45.0	1
GTCGTTG	20	7.032555E-4	45.0	1
TCCCGAG	20	7.032555E-4	45.0	44
>>END_MODULE