##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441451
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48129237446511	34.0	31.0	34.0	31.0	34.0
2	32.64993170249926	34.0	31.0	34.0	31.0	34.0
3	32.7094037616859	34.0	31.0	34.0	31.0	34.0
4	36.13173149454866	37.0	37.0	37.0	35.0	37.0
5	36.00999204894767	37.0	35.0	37.0	35.0	37.0
6	35.98770871512354	37.0	35.0	37.0	35.0	37.0
7	36.16181637373117	37.0	36.0	37.0	35.0	37.0
8	36.17951709249724	37.0	37.0	37.0	35.0	37.0
9	37.97719339179207	39.0	39.0	39.0	35.0	39.0
10	37.54155047785598	39.0	37.0	39.0	35.0	39.0
11	37.39491359176896	39.0	37.0	39.0	34.0	39.0
12	37.242622624028485	39.0	37.0	39.0	34.0	39.0
13	37.08652828966295	39.0	37.0	39.0	33.0	39.0
14	38.30735234488086	40.0	37.0	41.0	33.0	41.0
15	38.39473916697436	40.0	37.0	41.0	34.0	41.0
16	38.536779846460874	40.0	38.0	41.0	34.0	41.0
17	38.561704470031785	40.0	38.0	41.0	34.0	41.0
18	38.08022181397256	39.0	37.0	40.0	34.0	41.0
19	37.5816659153564	37.0	37.0	40.0	34.0	41.0
20	37.00116660739244	37.0	35.0	40.0	34.0	41.0
21	36.92830914416322	37.0	35.0	40.0	34.0	41.0
22	36.75243685029596	37.0	35.0	40.0	33.0	41.0
23	36.643154053337746	37.0	35.0	40.0	33.0	41.0
24	36.44251117338051	36.0	35.0	40.0	33.0	41.0
25	36.308709233867404	36.0	35.0	40.0	33.0	41.0
26	36.44354639586273	36.0	35.0	40.0	33.0	41.0
27	36.48399709140992	36.0	35.0	40.0	33.0	41.0
28	36.41749140901255	36.0	35.0	40.0	33.0	41.0
29	36.30466801524971	37.0	35.0	40.0	33.0	41.0
30	36.009885581865255	37.0	35.0	40.0	32.0	41.0
31	35.381394537559096	36.0	35.0	40.0	30.0	41.0
32	34.60570935392603	36.0	35.0	40.0	22.0	41.0
33	33.618929394202304	37.0	34.0	40.0	15.0	41.0
34	32.75861647159028	36.0	33.0	40.0	10.0	41.0
35	32.34093478098362	37.0	32.0	40.0	8.0	41.0
36	32.15206444203321	36.0	31.0	40.0	8.0	41.0
37	31.928499425757334	36.0	31.0	40.0	8.0	41.0
38	31.845423387873172	36.0	30.0	40.0	8.0	41.0
39	31.814117535128474	36.0	30.0	40.0	7.0	41.0
40	31.678054869056815	36.0	30.0	40.0	7.0	41.0
41	31.53866227508829	36.0	29.0	40.0	7.0	41.0
42	31.385270392410483	36.0	29.0	40.0	7.0	41.0
43	31.22559468661301	35.0	27.0	40.0	7.0	41.0
44	31.103051074751217	35.0	26.0	40.0	7.0	41.0
45	31.073052275337467	35.0	26.0	40.0	7.0	41.0
46	31.014642621717925	35.0	26.0	40.0	7.0	41.0
47	30.943182822102568	35.0	26.0	40.0	7.0	41.0
48	30.89288505406036	35.0	26.0	40.0	7.0	41.0
49	30.76058271472938	35.0	25.0	40.0	7.0	41.0
50	30.64016164874471	35.0	24.0	40.0	7.0	41.0
51	30.049677087604287	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	3.0
12	4.0
13	5.0
14	9.0
15	12.0
16	34.0
17	93.0
18	191.0
19	351.0
20	602.0
21	911.0
22	1497.0
23	2708.0
24	5260.0
25	11339.0
26	19715.0
27	23215.0
28	20068.0
29	15254.0
30	11801.0
31	10780.0
32	11311.0
33	14309.0
34	26703.0
35	39618.0
36	30594.0
37	39269.0
38	71377.0
39	84359.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.721758473760396	16.375090327125776	18.590964795639835	35.312186403474
2	50.98346135811222	18.35356585442099	17.96620689498948	12.696765892477307
3	22.929611666980026	18.988743937605758	45.00612752038165	13.075516875032564
4	20.93301408310322	19.909117886243322	45.501765767888166	13.656102262765291
5	19.721554600623854	21.710903361868024	44.37751868270771	14.190023354800418
6	19.72925647467103	24.909672874226132	44.111124450958314	11.249946200144523
7	58.056500041907256	4.8213731535323285	32.87703505032268	4.2450917542377296
8	58.49256202840179	4.089695119050585	32.474045817089554	4.943697035458069
9	54.789546291660905	5.266269642610392	34.20107780931519	5.743106256413509
10	32.0554263100548	17.559366724732755	39.30311631415491	11.082090651057536
11	25.73490602581034	18.10031011369325	42.188827299066034	13.975956561430374
12	23.664008009949008	16.166233625022937	46.26198604148592	13.907772323542137
13	17.300674367030542	22.03574122609304	46.567116169178455	14.096468237697954
14	14.043008170782262	25.347320540671557	45.68729032214221	14.922380966403972
15	12.151745040785954	19.93177045697031	54.571628561267275	13.34485594097646
16	13.860201925015462	18.766069167359458	51.3493003753531	16.024428532271983
17	14.717828252739263	17.744211701864987	45.95073971969709	21.58722032569866
18	16.291275815435917	19.144367098500172	48.72500005663143	15.839357029432485
19	18.543620922820423	20.15014123877848	45.45057095804517	15.855666880355917
20	19.716571035063915	19.525156812420857	46.13286638834208	14.625405764173147
21	16.38528398395292	21.201673571925312	46.47401410349053	15.939028340631237
22	14.894971355824316	20.461614086274583	45.054604021737404	19.5888105361637
23	14.215394234014648	21.867659151298785	45.534838521149574	18.382108093536996
24	16.432854382479594	18.699017558007572	47.67097594070463	17.197152118808205
25	14.030322731175145	20.227839556372054	46.77008320289228	18.97175450956052
26	13.895766461056832	22.586198694758874	45.64198518068823	17.87604966349606
27	16.60456086859017	21.993607444540846	45.469146066041304	15.93268562082768
28	12.976525140955623	21.6132707820347	48.27330779633527	17.136896280674414
29	14.107114945939639	19.426844655465725	48.170238599527465	18.295801799067167
30	16.579869566497756	20.799363915813988	45.838382968891224	16.782383548797036
31	17.065540682884397	24.24278119202358	41.42271735707927	17.268960768012757
32	17.639783350813566	23.25082511988873	40.93772581781444	18.171665711483268
33	19.12828377328401	23.136429637717438	39.767720539765456	17.967566049233096
34	15.702762027948742	25.01681953376479	38.75084663983092	20.52957179845555
35	16.559935304258	23.142545831813724	36.90103771426501	23.39648114966327
36	20.492648108170556	25.31832525014101	35.1647181680413	19.02430847364713
37	18.39366090460776	28.040258148696008	36.406985146709374	17.15909579998686
38	17.374748273307798	28.045921291377752	34.411067139954376	20.168263295360074
39	18.670022267477023	25.415504778559793	36.988929688685715	18.92554326527746
40	19.626187277863227	23.849759089910318	36.38478562739692	20.13926800482953
41	17.719067348358028	25.098368788381947	33.29882591725922	23.883737946000803
42	20.58393796820032	24.418338615157744	32.91350568919314	22.0842177274488
43	21.094073860972113	24.640786859696775	33.308113471257286	20.95702580807383
44	17.787025060538994	25.751668928148312	34.28874325802864	22.172562753284055
45	16.963604114612945	26.027577239603033	33.43927185576655	23.569546790017466
46	19.251740283746102	25.1792384658773	34.55264570699806	21.01637554337854
47	16.651678215702308	25.745099682637484	36.01169778752342	21.59152431413679
48	18.14697440939085	22.316633103107705	36.037521718152185	23.49887076934926
49	17.94083601577525	21.53376025878297	38.48196062530157	22.04344310014022
50	17.068712042786174	22.8199732246614	37.02879821316522	23.0825165193872
51	16.196135018382563	22.769004940525676	34.175480404393696	26.859379636698073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	354.0
2	384.0
3	13695.5
4	27007.0
5	18712.5
6	10418.0
7	10679.0
8	10940.0
9	11324.5
10	11709.0
11	11548.5
12	11388.0
13	10836.0
14	10284.0
15	9591.5
16	8899.0
17	8161.0
18	7423.0
19	6745.0
20	6067.0
21	5555.5
22	5044.0
23	4626.0
24	4208.0
25	3848.5
26	3683.0
27	3877.0
28	3991.0
29	4105.0
30	4678.0
31	5251.0
32	6109.0
33	6967.0
34	7046.5
35	7126.0
36	8086.0
37	9046.0
38	9750.0
39	10454.0
40	12242.5
41	14031.0
42	17940.5
43	21850.0
44	24922.0
45	27994.0
46	31257.0
47	34520.0
48	37142.5
49	39765.0
50	39754.5
51	39744.0
52	32661.5
53	25579.0
54	21633.0
55	17687.0
56	15655.0
57	13623.0
58	12532.5
59	11442.0
60	10886.5
61	10331.0
62	8909.5
63	7488.0
64	6312.5
65	5137.0
66	4139.0
67	3141.0
68	2522.0
69	1903.0
70	1625.5
71	1348.0
72	968.5
73	589.0
74	431.5
75	304.0
76	334.0
77	200.5
78	67.0
79	78.5
80	90.0
81	89.0
82	88.0
83	48.5
84	9.0
85	6.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.53421371526672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20116889347219	30.134205549247252
2	9.808677779504043	7.5593937163919325
3	3.7581508344870285	4.344521622909935
4	1.8261394884671724	2.814753972899275
5	1.0560019622906682	2.034610264932482
6	0.6192792355505506	1.4318063047279153
7	0.4467829427021742	1.2051500578894945
8	0.32463998601833305	1.0007797281401318
9	0.2734175010621889	0.9482335577482098
>10	3.251134807400506	31.404635297660665
>50	0.3875092865285834	9.218323645984828
>100	0.042327937451583725	2.8433521510791793
>500	0.0029808406656044876	0.8086450328673112
>1k	0.001192336266241795	0.8892798074515231
>5k	0.0	0.0
>10k+	5.961681331208976E-4	3.3623092900698768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14636	3.315430251602103	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	2041	0.4623389685378445	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1830	0.41454204430389785	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	994	0.22516655302627017	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	798	0.18076751440137184	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	651	0.1474682354326981	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	549	0.12436261329116935	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	528	0.11960557343850167	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2652570726988954E-4	0.0	0.0	0.24283555819332156	0.0
2	2.2652570726988954E-4	0.0	0.0	1.515230455928291	0.0
3	2.2652570726988954E-4	0.0	0.0	2.1707958527673514	0.0
4	2.2652570726988954E-4	0.0	0.0	2.8261347238991417	0.0
5	2.2652570726988954E-4	0.0	0.0	4.542066956468555	0.0
6	2.2652570726988954E-4	0.0	0.0	5.379079444830797	0.0
7	2.2652570726988954E-4	0.0	0.0	6.309647050295503	0.0
8	2.2652570726988954E-4	0.0	0.0	7.703006675712594	0.0
9	2.2652570726988954E-4	0.0	0.0	8.207479425802637	0.0
10	2.2652570726988954E-4	0.0	0.0	9.301825117623473	0.0
11	2.2652570726988954E-4	0.0	0.0	10.853979263836756	0.0
12	2.2652570726988954E-4	0.0	0.0	12.109158207819215	0.0
13	2.2652570726988954E-4	0.0	0.0	12.608420866642051	0.0
14	2.2652570726988954E-4	0.0	0.0	12.785110918312565	0.0
15	2.2652570726988954E-4	0.0	0.0	13.118556759413842	0.0
16	2.2652570726988954E-4	0.0	0.0	13.900750026616771	0.0
17	2.2652570726988954E-4	0.0	0.0	14.856688511295705	0.0
18	2.2652570726988954E-4	0.0	0.0	16.088308781722095	0.0
19	2.2652570726988954E-4	0.0	0.0	16.69449157437632	0.0
20	2.2652570726988954E-4	0.0	0.0	17.273264756450885	0.0
21	2.2652570726988954E-4	0.0	0.0	17.98568810581469	0.0
22	2.2652570726988954E-4	0.0	0.0	18.69561967239852	0.0
23	2.2652570726988954E-4	0.0	0.0	19.433640426683823	0.0
24	2.2652570726988954E-4	0.0	0.0	19.963484055988094	0.0
25	2.2652570726988954E-4	0.0	0.0	20.455044840763755	0.0
26	2.2652570726988954E-4	0.0	0.0	20.91693075788706	0.0
27	2.2652570726988954E-4	0.0	0.0	21.359788515599693	0.0
28	2.2652570726988954E-4	0.0	0.0	21.81850307282122	0.0
29	2.2652570726988954E-4	0.0	0.0	22.262493459070203	0.0
30	2.2652570726988954E-4	0.0	0.0	22.791430985545393	0.0
31	2.2652570726988954E-4	0.0	0.0	23.29635678704998	0.0
32	2.2652570726988954E-4	0.0	0.0	23.757563127051473	0.0
33	2.2652570726988954E-4	0.0	0.0	24.21718378710208	0.0
34	2.2652570726988954E-4	0.0	0.0	24.650300939402108	0.0
35	4.530514145397791E-4	0.0	0.0	25.125098821839796	0.0
36	4.530514145397791E-4	0.0	0.0	25.600123229984757	0.0
37	4.530514145397791E-4	0.0	0.0	26.028936393846656	0.0
38	4.530514145397791E-4	0.0	0.0	26.442798861028745	0.0
39	6.795771218096686E-4	0.0	0.0	26.88135263030325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.0285593E-4	45.000004	6
AACCGCA	20	7.0285593E-4	45.000004	24
CCGGGTG	20	7.0285593E-4	45.000004	5
CGTGCAG	20	7.0285593E-4	45.000004	38
TTAGCAC	20	7.0285593E-4	45.000004	14
TAGGTCG	20	7.0285593E-4	45.000004	1
TAGCGTC	20	7.0285593E-4	45.000004	14
CGGCACA	20	7.0285593E-4	45.000004	45
GTTAGCG	20	7.0285593E-4	45.000004	1
GATCAGA	20	7.0285593E-4	45.000004	9
CGCGATT	20	7.0285593E-4	45.000004	12
ATCACGT	20	7.0285593E-4	45.000004	16
TCCTACT	20	7.0285593E-4	45.000004	11
TATGAGT	20	7.0285593E-4	45.000004	21
GGCCATA	20	7.0285593E-4	45.000004	14
CCATAGG	20	7.0285593E-4	45.000004	2
AGCGATG	20	7.0285593E-4	45.000004	1
ATGACTC	20	7.0285593E-4	45.000004	45
GCGATAT	40	6.7993824E-9	45.000004	9
TCGTAGG	20	7.0285593E-4	45.000004	2
>>END_MODULE