##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1720062
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.653827013212315	34.0	31.0	34.0	31.0	34.0
2	32.80292977811265	34.0	31.0	34.0	31.0	34.0
3	32.85014028564087	34.0	31.0	34.0	31.0	34.0
4	36.23690832074658	37.0	37.0	37.0	35.0	37.0
5	36.13432306509882	37.0	37.0	37.0	35.0	37.0
6	36.12373216779395	37.0	36.0	37.0	35.0	37.0
7	36.24807129045348	37.0	37.0	37.0	35.0	37.0
8	36.224843057982795	37.0	37.0	37.0	35.0	37.0
9	37.99626815777571	39.0	39.0	39.0	35.0	39.0
10	37.639743799932795	39.0	38.0	39.0	35.0	39.0
11	37.53515163988275	39.0	37.0	39.0	35.0	39.0
12	37.47641538502682	39.0	37.0	39.0	35.0	39.0
13	37.456855043597265	39.0	37.0	39.0	35.0	39.0
14	38.77190531504097	40.0	38.0	41.0	35.0	41.0
15	38.785215881753096	40.0	38.0	41.0	35.0	41.0
16	38.871460447355965	40.0	38.0	41.0	35.0	41.0
17	38.854237230983536	40.0	38.0	41.0	35.0	41.0
18	38.34572242163364	39.0	38.0	41.0	35.0	41.0
19	37.83935753478654	38.0	37.0	41.0	35.0	41.0
20	37.25063747702118	38.0	35.0	41.0	34.0	41.0
21	37.221129819739055	38.0	35.0	41.0	34.0	41.0
22	37.03219244422585	38.0	35.0	41.0	34.0	41.0
23	36.91094390783588	38.0	35.0	40.0	33.0	41.0
24	36.724054132932416	37.0	35.0	40.0	33.0	41.0
25	36.59317338561052	37.0	35.0	40.0	33.0	41.0
26	36.69646036014981	37.0	35.0	40.0	33.0	41.0
27	36.72123156025771	38.0	35.0	40.0	33.0	41.0
28	36.62301824004018	38.0	35.0	40.0	33.0	41.0
29	36.481301255419865	38.0	35.0	40.0	33.0	41.0
30	36.173645484872054	38.0	35.0	40.0	32.0	41.0
31	35.66154185139838	38.0	35.0	40.0	30.0	41.0
32	34.88148392325393	38.0	35.0	40.0	22.0	41.0
33	33.89758508704919	38.0	34.0	40.0	16.0	41.0
34	33.083528384441955	38.0	33.0	41.0	10.0	41.0
35	32.57516705793163	38.0	32.0	41.0	10.0	41.0
36	32.323068005688164	38.0	31.0	41.0	8.0	41.0
37	32.07491125319901	37.0	31.0	41.0	8.0	41.0
38	31.915153058436264	37.0	30.0	40.0	7.0	41.0
39	31.84841883606521	37.0	29.0	40.0	7.0	41.0
40	31.726506370119218	37.0	29.0	40.0	7.0	41.0
41	31.619577666386444	37.0	29.0	40.0	7.0	41.0
42	31.48433021600384	37.0	27.0	40.0	7.0	41.0
43	31.37298829925898	37.0	27.0	40.0	7.0	41.0
44	31.275748199774196	36.0	25.0	40.0	7.0	41.0
45	31.244795827127163	36.0	25.0	40.0	7.0	41.0
46	31.197974259067404	36.0	25.0	40.0	7.0	41.0
47	31.180443495641438	36.0	25.0	40.0	7.0	41.0
48	31.12013113480793	35.0	25.0	40.0	7.0	41.0
49	30.99544667575936	35.0	24.0	40.0	7.0	41.0
50	30.898308898167624	35.0	24.0	40.0	7.0	41.0
51	30.363060168761358	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	16.0
11	22.0
12	30.0
13	45.0
14	55.0
15	109.0
16	179.0
17	319.0
18	715.0
19	1290.0
20	2297.0
21	3786.0
22	5959.0
23	10032.0
24	18415.0
25	38945.0
26	70448.0
27	89340.0
28	82871.0
29	66054.0
30	51323.0
31	45034.0
32	42530.0
33	49440.0
34	87894.0
35	109818.0
36	113438.0
37	148233.0
38	277655.0
39	403479.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.817726337771546	14.969518540610746	17.51599651640464	36.69675860521307
2	50.94764025947902	19.192389576654794	17.369664581858096	12.49030558200809
3	23.223988437626087	18.981118122486283	45.47336084396958	12.321532595918054
4	20.96854648262679	18.605724677366283	47.0923722516979	13.333356588309025
5	20.165668446835056	21.588465997155918	43.85940739345442	14.386458162554606
6	20.113344751526398	25.675586112593617	43.436806347678164	10.77426278820182
7	58.18941410251492	4.705469919107567	33.68413464165826	3.420981336719258
8	58.90828353861664	5.478581586012597	32.15727107511241	3.455863800258363
9	54.098980153041	5.967924412027008	33.804072178793554	6.129023256138442
10	31.325673144340143	18.789671535095827	38.79528761172563	11.089367708838402
11	24.78712976625261	18.01121122378147	43.14210766821196	14.05955134175396
12	21.836771000115114	16.199416067560353	46.91499492460156	15.048818007722979
13	19.603246859706218	16.9564818012374	48.1684962518793	15.271775087177089
14	16.079711080181994	20.00259292978974	47.07086139918212	16.846834590846143
15	15.625308855145919	18.3620125321064	51.09048394767165	14.922194665076027
16	17.57907563797119	17.749185785163558	48.75655644970937	15.91518212715588
17	17.841275488906795	18.05987226041852	47.048071523003244	17.050780727671444
18	17.8644723271603	18.94844488163799	47.05266438070256	16.13441841049916
19	18.623863558406615	19.839168588109032	45.95543649008001	15.581531363404341
20	20.453797595668064	18.907690536736467	46.55727526100804	14.081236606587435
21	19.4987738814066	19.46534485384829	45.91590303140236	15.119978233342751
22	17.47547472126005	18.839088358442893	46.37670037475393	17.308736545543127
23	17.60808622014788	19.359360302128646	46.777674293135945	16.25487918458753
24	17.8891226013946	17.867379199121892	47.00301500759856	17.240483191884945
25	17.1460098531332	19.556795045759976	45.738700116623704	17.558494984483115
26	16.17023107306597	19.6935924402725	47.18457823031961	16.951598256341924
27	17.71296616052212	19.56156231577699	46.002702228175494	16.722769295525396
28	15.288286119918935	20.080962197874264	47.77415000156971	16.85660168063709
29	17.180834179233074	18.7673467584308	46.69918875017296	17.352630312163168
30	17.880576397827518	19.908061453598766	45.52208001804586	16.689282130527854
31	17.897436255204756	20.192586081199398	44.015797104988074	17.894180558607772
32	19.142682066111572	21.322603487548704	42.7523542756017	16.78236017073803
33	19.203668239865774	22.670868840774343	40.25273507582866	17.872727843531223
34	17.91092414110654	23.553569580631397	39.38724301798423	19.14826326027783
35	18.613456956784116	24.190988464369305	37.24795966657016	19.94759491227642
36	19.6755116966714	27.051524886893613	35.67795812011427	17.595005296320714
37	20.194330204376353	26.244460955477187	35.500813342774855	18.060395497371605
38	19.10634616659167	26.59677383722215	34.396201997369865	19.90067799881632
39	20.061137331096205	25.513266382258315	34.53049948199542	19.895096804650063
40	19.452147655142664	24.91811341684195	36.38979292606894	19.239946001946443
41	18.644444211894687	25.670876980015837	34.724504116712076	20.960174691377404
42	18.636479382719926	25.86778848669408	35.47918621538061	20.016545915205384
43	20.24031691880874	25.388852262302176	34.35283146770291	20.017999351186177
44	19.09181180678371	25.293041762448098	34.67840112740122	20.936745303366973
45	18.37457021898048	24.586439326024294	34.50404694714493	22.5349435078503
46	19.095532602894547	25.42001392973044	35.03396970574317	20.450483761631848
47	18.101033567394666	24.765560776297598	37.33080551747553	19.802600138832204
48	18.48375232985788	23.413981589035743	36.91925058515332	21.183015495953054
49	18.2100994033936	22.671275802848967	38.18478636235205	20.93383843140538
50	17.545821022730575	22.58494170558968	38.09287107092651	21.776366200753227
51	17.29309757438976	22.764411980498377	36.57118173647229	23.37130870863957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	410.0
1	920.0
2	1430.0
3	68250.5
4	135071.0
5	94415.0
6	53759.0
7	50897.5
8	48036.0
9	45926.5
10	43817.0
11	41719.5
12	39622.0
13	37264.0
14	34906.0
15	32370.0
16	29834.0
17	27502.5
18	25171.0
19	23021.5
20	20872.0
21	19079.0
22	17286.0
23	16236.0
24	15186.0
25	15187.0
26	15274.5
27	15361.0
28	17277.0
29	19193.0
30	20812.0
31	22431.0
32	23817.5
33	25204.0
34	27418.0
35	29632.0
36	32491.5
37	35351.0
38	39282.5
39	43214.0
40	49014.5
41	54815.0
42	60176.0
43	65537.0
44	72755.5
45	79974.0
46	97313.5
47	114653.0
48	112776.0
49	110899.0
50	111610.0
51	112321.0
52	99829.0
53	87337.0
54	80519.0
55	73701.0
56	71006.5
57	68312.0
58	66863.0
59	65414.0
60	64030.5
61	62647.0
62	58538.0
63	54429.0
64	45437.5
65	36446.0
66	29567.5
67	22689.0
68	19009.5
69	15330.0
70	12078.5
71	8827.0
72	7586.0
73	6345.0
74	5544.0
75	3565.0
76	2387.0
77	1785.5
78	1184.0
79	882.0
80	580.0
81	407.0
82	234.0
83	175.5
84	117.0
85	98.5
86	80.0
87	71.0
88	62.0
89	37.0
90	12.0
91	9.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1720062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.22796834194038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70182924105205	25.36400061233423
2	8.981502874220105	5.7891118058682425
3	3.178668397272709	3.0732607343049376
4	1.5301335261977684	1.9725237936497313
5	0.9453171875913923	1.5232826197393754
6	0.6117860806715901	1.1829973463954275
7	0.4606608673103354	1.0392314693633802
8	0.3433675620749299	0.8852831136160071
9	0.3035308088289397	0.8803963167968365
>10	4.240111753523551	34.42093674482189
>50	0.618852578992595	12.313814947993341
>100	0.07592605473474819	4.682410964254796
>500	0.005726779853560918	1.3105722155863855
>1k	0.0020320831738441965	1.2534173831254336
>5k	3.694696679716721E-4	0.6647821450614446
>10k+	1.8473483398583605E-4	3.643977787088552
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61206	3.5583601056240997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	5596	0.3253371099413858	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	5570	0.323825536521358	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	4226	0.24568881819376276	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	3572	0.20766693293613833	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	2505	0.14563428527576333	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1743	0.10133355658109998	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.813743923184164E-5	0.0	0.0	0.08371791249385196	0.0
2	5.813743923184164E-5	0.0	0.0	0.5616076629795903	0.0
3	5.813743923184164E-5	0.0	0.0	0.7999130263909091	0.0
4	5.813743923184164E-5	0.0	0.0	1.0883909998593073	0.0
5	5.813743923184164E-5	0.0	0.0	2.026147894668913	0.0
6	5.813743923184164E-5	0.0	0.0	2.4331099692918046	0.0
7	5.813743923184164E-5	0.0	0.0	2.8830356115070273	0.0
8	5.813743923184164E-5	0.0	0.0	3.5188266469464473	0.0
9	5.813743923184164E-5	0.0	0.0	3.761957417814009	0.0
10	1.1627487846368328E-4	0.0	0.0	4.624891428332234	0.0
11	1.1627487846368328E-4	0.0	0.0	5.396084559742614	0.0
12	1.1627487846368328E-4	0.0	0.0	6.350294349854831	0.0
13	1.1627487846368328E-4	0.0	0.0	6.617900982638998	0.0
14	1.1627487846368328E-4	0.0	0.0	6.732722425121885	0.0
15	1.1627487846368328E-4	0.0	0.0	6.979806541857212	0.0
16	1.1627487846368328E-4	0.0	0.0	7.421941767215368	0.0
17	1.1627487846368328E-4	0.0	0.0	7.927214251579303	0.0
18	1.1627487846368328E-4	0.0	0.0	8.495217032874397	0.0
19	1.1627487846368328E-4	0.0	0.0	8.940666092268767	0.0
20	1.7441231769552493E-4	0.0	0.0	9.310129518587122	0.0
21	2.3254975692736657E-4	0.0	0.0	9.728660943617149	0.0
22	2.3254975692736657E-4	0.0	0.0	10.202190386160499	0.0
23	2.3254975692736657E-4	0.0	0.0	10.649906805684912	0.0
24	2.3254975692736657E-4	0.0	0.0	11.01506806150011	0.0
25	2.3254975692736657E-4	0.0	0.0	11.353020995754804	0.0
26	2.3254975692736657E-4	0.0	0.0	11.65940530050661	0.0
27	2.3254975692736657E-4	0.0	0.0	11.967417453556907	0.0
28	2.3254975692736657E-4	0.0	0.0	12.264209080835458	0.0
29	2.3254975692736657E-4	0.0	0.0	12.583848721732124	0.0
30	2.906871961592082E-4	0.0	0.0	12.939591712391763	0.0
31	3.4882463539104985E-4	0.0	0.0	13.28202122946731	0.0
32	3.4882463539104985E-4	0.0	0.0	13.612358159182634	0.0
33	3.4882463539104985E-4	0.0	0.0	13.94310205097258	0.0
34	3.4882463539104985E-4	0.0	0.0	14.267101999811635	0.0
35	4.069620746228915E-4	0.0	0.0	14.599124915264682	0.0
36	4.6509951385473314E-4	0.0	0.0	14.934577939632408	0.0
37	4.6509951385473314E-4	0.0	0.0	15.266658992524688	0.0
38	4.6509951385473314E-4	0.0	0.0	15.594844836988434	0.0
39	4.6509951385473314E-4	0.0	0.0	15.942739273351775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACT	30	2.1661326E-6	45.000004	32
TATAGCG	20	7.034265E-4	45.0	1
TACGTAG	40	6.8175723E-9	45.0	1
CGTTTTT	47145	0.0	44.45116	1
TAGTACG	60	3.6379788E-12	41.250004	1
GCGTAAG	95	0.0	40.26316	1
TCTAGCG	45	1.9292202E-8	40.0	1
GTTTTTT	53375	0.0	39.50726	2
TACGTTG	40	3.459554E-7	39.375	1
TAGGGCG	495	0.0	37.272728	5
TGCGACG	55	2.750312E-9	36.81818	1
CGTACTG	55	2.750312E-9	36.81818	1
TGTAACG	55	2.750312E-9	36.81818	1
TATTGCG	80	0.0	36.5625	1
ACGGGCG	250	0.0	36.000004	5
CGAACTA	25	0.002107399	36.0	13
ACGCAGT	25	0.002107399	36.0	38
GCGAACC	25	0.002107399	36.0	33
CGCGCGA	25	0.002107399	36.0	14
CGATTCA	25	0.002107399	36.0	10
>>END_MODULE