##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934925.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465899
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.296422615202005	33.0	31.0	34.0	30.0	34.0
2	32.48621053060857	34.0	31.0	34.0	31.0	34.0
3	32.56016218107358	34.0	31.0	34.0	31.0	34.0
4	36.025597822704064	37.0	35.0	37.0	35.0	37.0
5	35.88184992884724	37.0	35.0	37.0	35.0	37.0
6	35.862886591299834	37.0	35.0	37.0	35.0	37.0
7	36.058345263673026	37.0	35.0	37.0	35.0	37.0
8	36.05669469133868	37.0	35.0	37.0	35.0	37.0
9	37.83670709746104	39.0	38.0	39.0	35.0	39.0
10	37.43302732995778	39.0	37.0	39.0	34.0	39.0
11	37.27980313329713	39.0	37.0	39.0	34.0	39.0
12	37.12896572003803	39.0	37.0	39.0	34.0	39.0
13	36.953588653334734	39.0	37.0	39.0	33.0	39.0
14	38.0774373844975	40.0	37.0	41.0	33.0	41.0
15	38.206544766140304	40.0	37.0	41.0	33.0	41.0
16	38.32560275939635	40.0	37.0	41.0	34.0	41.0
17	38.36264941543124	40.0	37.0	41.0	34.0	41.0
18	37.95190159240522	39.0	37.0	40.0	34.0	41.0
19	37.53469743442248	38.0	37.0	40.0	34.0	41.0
20	37.017669065612935	38.0	35.0	40.0	34.0	41.0
21	36.967458612274335	38.0	35.0	40.0	34.0	41.0
22	36.73731645700034	37.0	35.0	40.0	33.0	41.0
23	36.62153385175757	37.0	35.0	40.0	33.0	41.0
24	36.38298000210346	37.0	35.0	40.0	33.0	41.0
25	36.240942779443614	37.0	35.0	40.0	32.0	41.0
26	36.43085303896338	37.0	35.0	40.0	33.0	41.0
27	36.526691407365114	37.0	35.0	40.0	33.0	41.0
28	36.53023938664818	37.0	35.0	40.0	33.0	41.0
29	36.48313690306268	37.0	35.0	40.0	33.0	41.0
30	36.23684961762098	37.0	35.0	40.0	32.0	41.0
31	35.745758200811764	37.0	35.0	40.0	31.0	41.0
32	35.160830995559124	37.0	35.0	40.0	26.0	41.0
33	34.34813768649428	37.0	35.0	40.0	20.0	41.0
34	33.720817172820716	37.0	34.0	40.0	15.0	41.0
35	33.323288953185134	38.0	34.0	40.0	10.0	41.0
36	33.12876610595859	37.0	33.0	40.0	10.0	41.0
37	32.93253473392302	37.0	33.0	40.0	10.0	41.0
38	32.8295982605672	37.0	33.0	40.0	10.0	41.0
39	32.779954453647676	37.0	33.0	40.0	10.0	41.0
40	32.63295692843299	37.0	33.0	40.0	10.0	41.0
41	32.48199931744863	37.0	32.0	40.0	10.0	41.0
42	32.366824139996005	37.0	32.0	40.0	8.0	41.0
43	32.23152872189036	37.0	31.0	40.0	8.0	41.0
44	32.10208435733925	37.0	31.0	40.0	8.0	41.0
45	32.060592531857765	37.0	31.0	40.0	8.0	41.0
46	31.986449852865107	36.0	31.0	40.0	8.0	41.0
47	31.91729537947066	36.0	31.0	40.0	8.0	41.0
48	31.910733871504338	36.0	31.0	40.0	8.0	41.0
49	31.833002431857548	36.0	31.0	40.0	8.0	41.0
50	31.72003803399449	36.0	30.0	40.0	8.0	41.0
51	31.085117160586307	35.0	28.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	1.0
13	7.0
14	13.0
15	24.0
16	45.0
17	79.0
18	171.0
19	321.0
20	614.0
21	994.0
22	1513.0
23	2658.0
24	4895.0
25	9752.0
26	16775.0
27	20067.0
28	17968.0
29	14361.0
30	12070.0
31	11345.0
32	12128.0
33	15640.0
34	29265.0
35	43068.0
36	36884.0
37	46596.0
38	84592.0
39	84005.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.07089519402274	17.46751978433094	19.47739746168161	32.98418755996471
2	47.31883949096263	18.80128525710508	19.2305628473124	14.649312404619884
3	23.392623723167468	18.891218912253514	44.022846153350834	13.693311211228185
4	22.095346845560947	19.64867922017433	42.847698750158294	15.408275184106426
5	18.930068534167276	22.53342462636752	42.32440936769557	16.21209747176963
6	19.08053032953494	24.302262936816778	42.54162382834048	14.075582905307805
7	57.92585946739529	5.228386409930049	31.334259142002878	5.511494980671777
8	56.8528801306721	4.093161822626793	31.6051333014237	7.448824745277411
9	52.261756303404816	6.066121627219633	33.95585738539898	7.71626468397657
10	30.723826408728073	18.889931079482892	38.92410157566339	11.46214093612564
11	23.347120298605493	18.477180676498556	42.38064473201273	15.79505429288322
12	21.321573989212254	16.646955670649646	47.42057827984177	14.61089206029633
13	16.306109264025036	23.278221245377217	47.13553795994411	13.280131530653637
14	12.546925406579538	25.854530703006446	44.942358751574915	16.656185138839106
15	10.807277972264375	20.587723948752842	53.36285332228658	15.242144756696193
16	11.932629174992863	20.36557279582055	49.663768327470116	18.038029701716464
17	12.128809033717609	21.13891637457904	42.93097860265851	23.801295989044835
18	14.34860345267966	20.81051901807044	47.45878398537022	17.38209354387968
19	15.200719469241186	21.36299927666726	44.52467165630319	18.911609597788363
20	17.899373040079503	21.64267362668733	44.50621271992428	15.951740613308893
21	15.63901188884286	22.5909478234553	45.09689868404955	16.673141603652294
22	13.61689980017128	21.980729728975593	43.439672547054194	20.962697923798935
23	12.61045848993022	22.383606747385162	43.86701838810558	21.13891637457904
24	17.360415025574213	18.70791738123499	46.06513428876215	17.866533304428643
25	12.8731763751371	22.20266624311278	43.381720072376204	21.54243730937392
26	11.935634118124314	24.02602280751837	42.838254643173734	21.200088431183584
27	14.694815829181863	22.12303525012932	43.29071322325225	19.89143569743657
28	11.006677412915675	19.977935131863344	47.52403417908173	21.49135327613925
29	11.522669076344872	18.14148560095643	45.81829967439295	24.51754564830575
30	13.99037130365165	20.206954726238948	44.40576176381577	21.39691220629364
31	15.68172500906849	23.42803912435957	40.14153282149135	20.748703045080585
32	16.778743890843295	22.97643909946147	37.416049401265084	22.82876760843015
33	15.506794391059007	24.343902863066887	39.141530675103404	21.007772070770702
34	14.04961161110026	24.44306598640478	38.88460803736432	22.62271436513064
35	15.654895159680532	23.252249951169674	34.85347682652249	26.239378062627306
36	18.030302705092733	24.998336549337946	35.60685899733633	21.364501748232986
37	16.440473149759928	25.874492110951085	38.17136332123486	19.513671418054127
38	16.52160661430911	27.287888576708685	33.46691021015285	22.72359459882936
39	17.550155720445847	25.74806986063503	37.033992345980565	19.667782072938557
40	19.72444671484592	24.48814013337655	35.217504223018295	20.569908928759236
41	17.29173061114104	25.74892841581545	31.877080654820034	25.082260318223476
42	19.032451239431722	24.248173960450657	32.534948561812755	24.18442623830487
43	19.700836447384518	23.72703096593897	33.564570861925006	23.0075617247515
44	16.635150536918946	23.913552078884052	34.37032489874415	25.080972485452858
45	16.884990094419607	23.67766404306513	32.42505349871968	27.012292363795588
46	20.02451175040084	22.932867424055427	34.615871680342735	22.426749145201
47	15.325853886786621	23.80043743386442	38.018111221530845	22.85559745781811
48	16.794412522885864	20.970639559217773	36.36560713802777	25.8693407798686
49	16.98715816088895	19.67507979197208	39.973685283720286	23.36407676341868
50	17.204587260328953	20.29109313391958	37.68627964430059	24.81803996145087
51	15.411065488442773	21.75063694062447	32.74593849739965	30.092359073533103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	668.0
1	648.0
2	628.0
3	15126.5
4	29625.0
5	19699.0
6	9773.0
7	9636.0
8	9499.0
9	9743.5
10	9988.0
11	9941.5
12	9895.0
13	9307.0
14	8719.0
15	8034.5
16	7350.0
17	6856.0
18	6362.0
19	5849.0
20	5336.0
21	4975.0
22	4614.0
23	4325.0
24	4036.0
25	4006.0
26	3966.5
27	3957.0
28	4411.5
29	4866.0
30	4751.5
31	4637.0
32	5237.5
33	5838.0
34	6283.0
35	6728.0
36	7792.5
37	8857.0
38	9360.5
39	9864.0
40	12549.5
41	15235.0
42	19453.5
43	23672.0
44	26443.0
45	29214.0
46	35286.5
47	41359.0
48	48729.5
49	56100.0
50	54311.0
51	52522.0
52	40921.0
53	29320.0
54	24007.5
55	18695.0
56	15827.0
57	12959.0
58	11011.5
59	9064.0
60	8238.5
61	7413.0
62	6152.5
63	4892.0
64	4546.0
65	4200.0
66	3212.5
67	2225.0
68	2008.0
69	1791.0
70	1308.0
71	825.0
72	698.5
73	572.0
74	402.0
75	201.0
76	170.0
77	138.5
78	107.0
79	86.5
80	66.0
81	52.0
82	38.0
83	22.5
84	7.0
85	4.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.877309172595005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84224361889446	27.59980557104646
2	10.961153052463864	8.084356600076866
3	4.5312186963956975	5.012974583868811
4	2.3363813521718724	3.4463782987651084
5	1.4107671592470838	2.601264835104914
6	0.872726026713426	1.931027250604885
7	0.6365380119452825	1.6431666346630607
8	0.43227782858782465	1.275299450663299
9	0.33815938344588126	1.1223367319653081
>10	3.2493941697979887	27.891890862524114
>50	0.32092243732028214	7.435989352363823
>100	0.05991343099694731	4.083100178332277
>500	0.004152416009689418	0.982875387154938
>1k	0.0035592137225909292	3.075942759578363
>5k	0.0	0.0
>10k+	5.932022870984882E-4	3.813591503287789
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17433	3.7417981150421014	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	4039	0.866926093423682	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3876	0.8319399698217853	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	2186	0.46920040609660035	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1676	0.35973462059373384	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	1155	0.2479078083447271	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1129	0.24232719967203195	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	977	0.2097021028162756	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	642	0.13779810645654958	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	640	0.13736882886634227	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	606	0.13007110983281783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	585	0.125563695135641	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	533	0.11440247779025067	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	510	0.10946578550286651	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	468	0.10045095610851278	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1463879510365982E-4	0.0	0.0	0.4958156166894542	0.0
2	2.1463879510365982E-4	0.0	0.0	2.9392636601495172	0.0
3	2.1463879510365982E-4	0.0	0.0	4.084146993232439	0.0
4	2.1463879510365982E-4	0.0	0.0	5.2395476272754395	0.0
5	4.2927759020731964E-4	0.0	0.0	8.178382009834749	0.0
6	4.2927759020731964E-4	0.0	0.0	9.53082105778291	0.0
7	4.2927759020731964E-4	0.0	0.0	11.080083880841126	0.0
8	4.2927759020731964E-4	0.0	0.0	13.17345604948712	0.0
9	4.2927759020731964E-4	0.0	0.0	13.882837267304716	0.0
10	6.439163853109794E-4	0.0	0.0	15.409992294467255	0.0
11	6.439163853109794E-4	0.0	0.0	17.866747943223746	0.0
12	6.439163853109794E-4	0.0	0.0	19.81931706228174	0.0
13	6.439163853109794E-4	0.0	0.0	20.52483478178747	0.0
14	6.439163853109794E-4	0.0	0.0	20.800860272290777	0.0
15	6.439163853109794E-4	0.0	0.0	21.343252507517725	0.0
16	6.439163853109794E-4	0.0	0.0	22.480623482772017	0.0
17	6.439163853109794E-4	0.0	0.0	23.899600557202312	0.0
18	6.439163853109794E-4	0.0	0.0	25.463888095917785	0.0
19	6.439163853109794E-4	0.0	0.0	26.33746799198968	0.0
20	6.439163853109794E-4	0.0	0.0	27.096001493886014	0.0
21	6.439163853109794E-4	0.0	0.0	28.010362761027604	0.0
22	6.439163853109794E-4	0.0	0.0	28.98546680718353	0.0
23	6.439163853109794E-4	0.0	0.0	29.901115907095743	0.0
24	6.439163853109794E-4	0.0	0.0	30.577872028057584	0.0
25	6.439163853109794E-4	0.0	0.0	31.157396774837466	0.0
26	6.439163853109794E-4	0.0	0.0	31.71781866885312	0.0
27	6.439163853109794E-4	0.0	0.0	32.2383177469795	0.0
28	6.439163853109794E-4	0.0	0.0	32.7721244304023	0.0
29	6.439163853109794E-4	0.0	0.0	33.34413681935355	0.0
30	6.439163853109794E-4	0.0	0.0	34.01166347212593	0.0
31	6.439163853109794E-4	0.0	0.0	34.58517833264291	0.0
32	6.439163853109794E-4	0.0	0.0	35.12284851437758	0.0
33	6.439163853109794E-4	0.0	0.0	35.596985612761564	0.0
34	6.439163853109794E-4	0.0	0.0	36.09537689499226	0.0
35	6.439163853109794E-4	0.0	0.0	36.657730538163854	0.0
36	6.439163853109794E-4	0.0	0.0	37.148394823770815	0.0
37	6.439163853109794E-4	0.0	0.0	37.62553686528625	0.0
38	6.439163853109794E-4	0.0	0.0	38.04687282007474	0.0
39	6.439163853109794E-4	0.0	0.0	38.49675573461201	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	20	7.028964E-4	45.0	28
TCGCCGT	20	7.028964E-4	45.0	31
AAGTACG	25	3.8872222E-5	45.0	1
CTGTCGG	40	6.7993824E-9	45.0	2
GATACGC	20	7.028964E-4	45.0	10
TGCCCGC	20	7.028964E-4	45.0	16
ATGATCG	20	7.028964E-4	45.0	27
GTACATC	25	3.8872222E-5	45.0	25
AGTCAAT	25	3.8872222E-5	45.0	30
ACGCATT	25	3.8872222E-5	45.0	11
GACACGC	20	7.028964E-4	45.0	22
CGGCACT	25	3.8872222E-5	45.0	17
GCAGTAG	40	6.7993824E-9	45.0	1
CGCGCAT	50	2.1827873E-11	45.0	36
TTACTCC	20	7.028964E-4	45.0	20
TTACTAG	20	7.028964E-4	45.0	1
TTGTACG	20	7.028964E-4	45.0	1
TAAGTAG	25	3.8872222E-5	45.0	1
GCGTTAG	25	3.8872222E-5	45.0	1
ACGGGTG	20	7.028964E-4	45.0	5
>>END_MODULE