SRR2934924.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934924.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1073462
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36807	3.428812570915412	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5758	0.5363953265229696	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4438	0.4134287007830738	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	2721	0.25347893078655787	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1987	0.18510203435240372	RNA PCR Primer, Index 35 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1550	0.1443926287097261	TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1371	0.127717609007119	TruSeq Adapter, Index 10 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	2.1653668E-6	45.000004	24
TAATACG	30	2.1653668E-6	45.000004	1
TGTTACG	30	2.1653668E-6	45.000004	1
GCGATAT	65	0.0	45.000004	9
CCGACGA	35	1.211829E-7	45.000004	29
GACGATA	30	2.1653668E-6	45.000004	9
TAGTGCG	30	2.1653668E-6	45.000004	1
CGCCCGC	30	2.1653668E-6	45.000004	25
CTATGCG	25	3.890633E-5	45.0	1
ACGTTAG	20	7.033079E-4	45.0	1
CGTTGTA	20	7.033079E-4	45.0	13
TAAACGC	25	3.890633E-5	45.0	39
GCAAACG	25	3.890633E-5	45.0	1
TCGATCA	20	7.033079E-4	45.0	17
GACCCGT	20	7.033079E-4	45.0	17
ACGATTG	20	7.033079E-4	45.0	1
CGTAGCG	25	3.890633E-5	45.0	1
GTCGTAC	20	7.033079E-4	45.0	13
GAACGAT	20	7.033079E-4	45.0	40
CGAGATA	25	3.890633E-5	45.0	19