##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934924.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1073462
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.479032327180654	34.0	31.0	34.0	31.0	34.0
2	32.64506521888991	34.0	31.0	34.0	31.0	34.0
3	32.71024405148948	34.0	31.0	34.0	31.0	34.0
4	36.128902560127884	37.0	37.0	37.0	35.0	37.0
5	36.00548319362959	37.0	35.0	37.0	35.0	37.0
6	35.9930644959952	37.0	35.0	37.0	35.0	37.0
7	36.145896175179	37.0	36.0	37.0	35.0	37.0
8	36.13861692356134	37.0	37.0	37.0	35.0	37.0
9	37.898422114616075	39.0	38.0	39.0	35.0	39.0
10	37.52244886172031	39.0	37.0	39.0	35.0	39.0
11	37.434993506989535	39.0	37.0	39.0	34.0	39.0
12	37.372180850370114	39.0	37.0	39.0	35.0	39.0
13	37.29763140194995	39.0	37.0	39.0	34.0	39.0
14	38.530956848030016	40.0	38.0	41.0	34.0	41.0
15	38.58423493332787	40.0	38.0	41.0	34.0	41.0
16	38.675135216710046	40.0	38.0	41.0	35.0	41.0
17	38.689538148532506	40.0	38.0	41.0	35.0	41.0
18	38.26778777450902	39.0	38.0	41.0	35.0	41.0
19	37.835558221902595	38.0	37.0	41.0	34.0	41.0
20	37.32076030637321	38.0	35.0	41.0	34.0	41.0
21	37.25557961064295	38.0	35.0	40.0	34.0	41.0
22	37.068106742483664	38.0	35.0	40.0	34.0	41.0
23	36.908820247013864	38.0	35.0	40.0	33.0	41.0
24	36.69017254453348	38.0	35.0	40.0	33.0	41.0
25	36.499332999211894	37.0	35.0	40.0	33.0	41.0
26	36.63055236235656	38.0	35.0	40.0	33.0	41.0
27	36.70148174784016	38.0	35.0	40.0	33.0	41.0
28	36.631646951638714	38.0	35.0	40.0	33.0	41.0
29	36.53611213065763	38.0	35.0	40.0	33.0	41.0
30	36.31395056369019	38.0	35.0	40.0	32.0	41.0
31	35.81866801060494	38.0	35.0	40.0	30.0	41.0
32	35.199693142374855	38.0	35.0	40.0	25.0	41.0
33	34.375673288854195	38.0	35.0	40.0	18.0	41.0
34	33.67667882048922	38.0	34.0	40.0	15.0	41.0
35	33.2651197713566	38.0	33.0	40.0	10.0	41.0
36	33.07982583454282	38.0	33.0	40.0	10.0	41.0
37	32.84691959286868	38.0	33.0	40.0	10.0	41.0
38	32.713899513909205	38.0	32.0	40.0	10.0	41.0
39	32.648785890883886	38.0	32.0	40.0	10.0	41.0
40	32.504239553891985	37.0	31.0	40.0	9.0	41.0
41	32.35752546433875	37.0	31.0	40.0	8.0	41.0
42	32.24255725866402	37.0	31.0	40.0	8.0	41.0
43	32.14991494808386	37.0	31.0	40.0	8.0	41.0
44	32.01600149795708	37.0	30.0	40.0	8.0	41.0
45	31.998034397118854	37.0	30.0	40.0	8.0	41.0
46	31.954527500740596	37.0	30.0	40.0	8.0	41.0
47	31.952281496690148	37.0	30.0	40.0	8.0	41.0
48	31.93881944586767	36.0	30.0	40.0	8.0	41.0
49	31.845702036960787	36.0	30.0	40.0	8.0	41.0
50	31.749628771209412	36.0	29.0	40.0	8.0	41.0
51	31.155390689190675	35.0	27.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	9.0
11	14.0
12	16.0
13	24.0
14	26.0
15	53.0
16	129.0
17	215.0
18	331.0
19	724.0
20	1235.0
21	1960.0
22	3182.0
23	5344.0
24	10285.0
25	21047.0
26	38469.0
27	49671.0
28	45596.0
29	36511.0
30	29009.0
31	25764.0
32	27048.0
33	33432.0
34	57669.0
35	80111.0
36	78400.0
37	105005.0
38	190448.0
39	231559.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.69917705517289	16.48898610290816	18.92009218770669	33.89174465421226
2	48.45909776033059	18.93164359800347	18.54066562207139	14.068593019594545
3	24.41250831422072	18.6482614195938	43.21727271202893	13.721957554156553
4	23.322204232660308	18.93574248552813	42.808967620651686	14.933085661159872
5	19.066906886317355	22.738112760395804	42.152586677497666	16.042393675789178
6	20.178450657778292	25.611619228254007	42.23978119393141	11.970148920036294
7	59.403220607715966	5.253003832459836	30.674770043094213	4.669005516729982
8	59.08341422425759	4.791226890192666	30.667969616064656	5.457389269485087
9	53.87531184149975	6.738384777476986	32.70148361097086	6.6848197700524095
10	30.26208659458835	19.717698437392286	38.05034551758702	11.96986945043234
11	23.41452235849988	19.2678455315605	41.67338946325068	15.644242646688939
12	21.429915544285684	16.775069820822722	46.36279626106933	15.432218373822268
13	18.367860250292978	20.470775863514497	46.87441194937501	14.286951936817513
14	14.649144543542295	23.08949920910102	45.1156165751559	17.145739672200786
15	13.861506043064404	19.967451106792787	50.7164669080042	15.454575942138613
16	15.307947556597254	19.944255129664583	47.45990076965929	17.287896544078876
17	15.779692248072125	19.384011730270842	44.64452397942358	20.191772042233445
18	16.56034400845116	19.98328771768353	46.29106572938772	17.165302544477587
19	17.508677531202782	20.769528870141656	44.05782412418884	17.663969474466725
20	18.87202341582655	20.833154783308586	44.31363196834168	15.981189832523182
21	17.360744954176297	21.232610003893942	45.49206213168235	15.914582910247404
22	16.46420646469088	20.237046118074044	43.175818054109044	20.122929363126037
23	15.795808328566824	20.83660157509069	44.791431834568904	18.57615826177359
24	17.430332885560922	19.06588216443619	45.268859074657506	18.23492587534538
25	15.440416148871595	20.59812084638301	44.03919281725855	19.92227018748684
26	14.352720450281426	21.212488192409232	44.954362613674256	19.48042874363508
27	16.072017453808332	20.904046906178326	45.184272941194	17.839662698819332
28	13.356318155649666	20.334208383715495	45.92784840078177	20.38162505985307
29	14.468141396714557	18.786692030085835	45.99082221820614	20.75434435499347
30	15.538789449463511	19.56212702452439	45.643814126629536	19.255269399382556
31	15.910577179257393	20.80558044905176	41.55517382077801	21.72866855091284
32	16.207094429052916	21.08840368825352	42.83207044124524	19.872431441448324
33	16.472031613601597	21.37756157181158	41.01067387574036	21.13973293884646
34	15.103562119572	21.139360312707854	39.60484861131554	24.152228956404606
35	15.77810858698305	21.843716871207363	39.106181681326404	23.271992860483184
36	17.306807320613117	23.43576204840041	36.88206941652336	22.375361214463112
37	16.802830468148848	24.44129368342801	37.88536529471933	20.87051055370381
38	16.27621657776428	25.26302747558833	36.452058852572335	22.008697094075057
39	17.6104976235768	23.362913638302988	37.16694209948746	21.85964663863276
40	18.232131179305835	23.111949933952015	37.13228786859712	21.52363101814503
41	15.802515599061728	24.32913321570768	34.63671746181979	25.231633723410795
42	16.6180079034004	23.774758677996985	35.21838686418336	24.388846554419253
43	18.832897671272946	22.772021739008927	35.68491478971775	22.71016580000037
44	17.480916883876656	24.00988577145721	34.912367647853394	23.596829696812744
45	17.251565495564815	23.058757552666048	34.694381356769036	24.9952955950001
46	17.796903849414324	23.15964607969355	36.86325179652377	22.18019827436835
47	15.99665381727532	22.797826099107375	38.65791243658369	22.547607647033615
48	16.133221297074325	21.164512577063743	38.370897153322616	24.331368972539316
49	16.724113196368386	20.67721074430208	39.54234057656442	23.056335482765107
50	16.496811251818883	20.87507522390173	38.5809651389616	24.047148385317783
51	15.865303103416794	21.268475269734747	35.574151670017194	27.29206995683126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	423.0
1	767.0
2	1111.0
3	36304.5
4	71498.0
5	50091.5
6	28685.0
7	27092.0
8	25499.0
9	24994.5
10	24490.0
11	23606.5
12	22723.0
13	21400.0
14	20077.0
15	18274.0
16	16471.0
17	15114.0
18	13757.0
19	12392.0
20	11027.0
21	10393.0
22	9759.0
23	9109.0
24	8459.0
25	9142.0
26	10311.5
27	10798.0
28	11548.5
29	12299.0
30	13662.0
31	15025.0
32	14313.0
33	13601.0
34	16856.0
35	20111.0
36	21783.5
37	23456.0
38	24636.0
39	25816.0
40	29438.5
41	33061.0
42	40671.5
43	48282.0
44	54486.0
45	60690.0
46	69630.5
47	78571.0
48	89430.5
49	100290.0
50	98816.5
51	97343.0
52	80050.5
53	62758.0
54	53799.5
55	44841.0
56	39899.0
57	34957.0
58	32174.0
59	29391.0
60	28636.5
61	27882.0
62	24863.0
63	21844.0
64	19268.0
65	16692.0
66	14028.0
67	11364.0
68	9805.5
69	8247.0
70	6607.5
71	4968.0
72	4240.5
73	3513.0
74	2628.5
75	1357.0
76	970.0
77	809.5
78	649.0
79	484.0
80	319.0
81	212.5
82	106.0
83	63.0
84	20.0
85	14.0
86	8.0
87	19.5
88	31.0
89	19.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1073462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.33004144540797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94241788978596	24.552215178412716
2	10.324189442529178	6.675629451344234
3	4.134008981031981	4.009580450773581
4	2.100879620490214	2.7168610080904636
5	1.213311601094862	1.9613207174795595
6	0.8027976054623335	1.557268791412291
7	0.5535433658337123	1.2527255971464182
8	0.41936895718133427	1.0846572613272047
9	0.3193411348000696	0.9291880910978878
>10	3.2425420830261587	25.626073512389265
>50	0.8513082850375225	17.941911658474922
>100	0.088340397914354	5.087955603508373
>500	0.005889359860919	1.40355754551008
>1k	0.00147233996522975	1.1487979991636799
>5k	2.9446799304595E-4	0.5481709521160578
>10k+	2.9446799304595E-4	3.504086181753341
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36807	3.428812570915412	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5758	0.5363953265229696	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4438	0.4134287007830738	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	2721	0.25347893078655787	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1987	0.18510203435240372	RNA PCR Primer, Index 35 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1550	0.1443926287097261	TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1371	0.127717609007119	TruSeq Adapter, Index 10 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7946960395430857E-4	0.0	0.0	0.27043342009311927	0.0
2	2.7946960395430857E-4	0.0	0.0	1.6164521892717207	0.0
3	2.7946960395430857E-4	0.0	0.0	2.3143809468802807	0.0
4	2.7946960395430857E-4	0.0	0.0	2.994423649835765	0.0
5	2.7946960395430857E-4	0.0	0.0	4.841717731973745	0.0
6	2.7946960395430857E-4	0.0	0.0	5.774866739577181	0.0
7	2.7946960395430857E-4	0.0	0.0	6.7089473125271315	0.0
8	2.7946960395430857E-4	0.0	0.0	8.084217233586285	0.0
9	2.7946960395430857E-4	0.0	0.0	8.594901356545458	0.0
10	2.7946960395430857E-4	0.0	0.0	9.725542217609938	0.0
11	3.7262613860574475E-4	0.0	0.0	11.415029130048385	0.0
12	4.65782673257181E-4	0.0	0.0	12.735802478336447	0.0
13	4.65782673257181E-4	0.0	0.0	13.242853496444216	0.0
14	4.65782673257181E-4	0.0	0.0	13.4527351690139	0.0
15	4.65782673257181E-4	0.0	0.0	13.847998345539944	0.0
16	4.65782673257181E-4	0.0	0.0	14.686872940076128	0.0
17	4.65782673257181E-4	0.0	0.0	15.651974639065006	0.0
18	4.65782673257181E-4	0.0	0.0	16.749358617258924	0.0
19	4.65782673257181E-4	0.0	0.0	17.328047010513647	0.0
20	4.65782673257181E-4	0.0	0.0	17.86593284159104	0.0
21	4.65782673257181E-4	0.0	0.0	18.56255740771448	0.0
22	4.65782673257181E-4	0.0	0.0	19.242972736808568	0.0
23	4.65782673257181E-4	0.0	0.0	19.893205348675593	0.0
24	4.65782673257181E-4	0.0	0.0	20.411435150941532	0.0
25	4.65782673257181E-4	0.0	0.0	20.920628769346283	0.0
26	5.589392079086171E-4	0.0	0.0	21.378120511019485	0.0
27	5.589392079086171E-4	0.0	0.0	21.807292666158652	0.0
28	5.589392079086171E-4	0.0	0.0	22.222491341100103	0.0
29	5.589392079086171E-4	0.0	0.0	22.643465721189944	0.0
30	5.589392079086171E-4	0.0	0.0	23.118843517516222	0.0
31	6.520957425600534E-4	0.0	0.0	23.5777326072092	0.0
32	6.520957425600534E-4	0.0	0.0	24.023952408189576	0.0
33	6.520957425600534E-4	0.0	0.0	24.43533166521032	0.0
34	7.452522772114895E-4	0.0	0.0	24.84270519124105	0.0
35	7.452522772114895E-4	0.0	0.0	25.269921059152537	0.0
36	7.452522772114895E-4	0.0	0.0	25.67887824627234	0.0
37	7.452522772114895E-4	0.0	0.0	26.10022525250079	0.0
38	7.452522772114895E-4	0.0	0.0	26.49558158556148	0.0
39	7.452522772114895E-4	0.0	0.0	26.868673506840484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	2.1653668E-6	45.000004	24
TAATACG	30	2.1653668E-6	45.000004	1
TGTTACG	30	2.1653668E-6	45.000004	1
GCGATAT	65	0.0	45.000004	9
CCGACGA	35	1.211829E-7	45.000004	29
GACGATA	30	2.1653668E-6	45.000004	9
TAGTGCG	30	2.1653668E-6	45.000004	1
CGCCCGC	30	2.1653668E-6	45.000004	25
CTATGCG	25	3.890633E-5	45.0	1
ACGTTAG	20	7.033079E-4	45.0	1
CGTTGTA	20	7.033079E-4	45.0	13
TAAACGC	25	3.890633E-5	45.0	39
GCAAACG	25	3.890633E-5	45.0	1
TCGATCA	20	7.033079E-4	45.0	17
GACCCGT	20	7.033079E-4	45.0	17
ACGATTG	20	7.033079E-4	45.0	1
CGTAGCG	25	3.890633E-5	45.0	1
GTCGTAC	20	7.033079E-4	45.0	13
GAACGAT	20	7.033079E-4	45.0	40
CGAGATA	25	3.890633E-5	45.0	19
>>END_MODULE