##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934923.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1736815
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.620942932897286	34.0	31.0	34.0	31.0	34.0
2	32.79359862737252	34.0	31.0	34.0	31.0	34.0
3	32.830742479769	34.0	31.0	34.0	31.0	34.0
4	36.225197272018036	37.0	37.0	37.0	35.0	37.0
5	36.12617809035505	37.0	35.0	37.0	35.0	37.0
6	36.11768668511039	37.0	36.0	37.0	35.0	37.0
7	36.28499350823202	37.0	37.0	37.0	35.0	37.0
8	36.315266162487085	37.0	37.0	37.0	35.0	37.0
9	38.13781030219108	39.0	39.0	39.0	37.0	39.0
10	37.55542875896396	39.0	38.0	39.0	34.0	39.0
11	37.50514015597516	39.0	37.0	39.0	35.0	39.0
12	37.562601658783464	39.0	37.0	39.0	35.0	39.0
13	37.60148662926103	39.0	37.0	39.0	35.0	39.0
14	38.954206406554526	40.0	38.0	41.0	35.0	41.0
15	38.994691432305686	40.0	38.0	41.0	35.0	41.0
16	39.072504555752914	41.0	38.0	41.0	35.0	41.0
17	39.091314849307494	41.0	39.0	41.0	35.0	41.0
18	38.49776919245861	39.0	38.0	41.0	35.0	41.0
19	37.87886907932048	38.0	37.0	40.0	35.0	41.0
20	37.22808416555592	38.0	35.0	40.0	34.0	41.0
21	37.18905064730556	38.0	35.0	40.0	34.0	41.0
22	37.02354827658674	38.0	35.0	40.0	34.0	41.0
23	36.90402719921235	37.0	35.0	40.0	34.0	41.0
24	36.696559506913516	37.0	35.0	40.0	33.0	41.0
25	36.56513675895245	37.0	35.0	40.0	33.0	41.0
26	36.71096173167551	37.0	35.0	40.0	33.0	41.0
27	36.756230226017166	37.0	35.0	40.0	34.0	41.0
28	36.691785250588005	37.0	35.0	40.0	33.0	41.0
29	36.54541502693148	37.0	35.0	40.0	33.0	41.0
30	36.2004364310534	37.0	35.0	40.0	33.0	41.0
31	35.57496739721847	37.0	35.0	40.0	30.0	41.0
32	34.60932223639248	37.0	35.0	40.0	21.0	41.0
33	33.315024916297936	37.0	33.0	40.0	15.0	41.0
34	32.19483998007848	37.0	31.0	40.0	8.0	41.0
35	31.528113241767258	37.0	27.0	40.0	7.0	41.0
36	31.20748093492974	37.0	24.0	40.0	7.0	41.0
37	30.972359750462772	37.0	23.0	40.0	7.0	41.0
38	30.834445810290674	37.0	23.0	40.0	7.0	41.0
39	30.76289702702936	37.0	23.0	40.0	7.0	41.0
40	30.685513425436792	37.0	22.0	40.0	7.0	41.0
41	30.56522312393663	37.0	22.0	40.0	7.0	41.0
42	30.429023816583804	37.0	21.0	40.0	7.0	41.0
43	30.328799555508215	37.0	21.0	40.0	7.0	41.0
44	30.21693617339786	36.0	20.0	40.0	7.0	41.0
45	30.15738463797238	36.0	20.0	40.0	7.0	41.0
46	30.070383431741433	36.0	20.0	40.0	7.0	41.0
47	30.016909112369483	36.0	20.0	40.0	7.0	41.0
48	29.925094497686857	36.0	20.0	40.0	7.0	41.0
49	29.781778715637532	35.0	20.0	40.0	7.0	41.0
50	29.674302098957	35.0	20.0	40.0	7.0	41.0
51	29.0637275702939	35.0	19.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	16.0
11	9.0
12	19.0
13	17.0
14	28.0
15	53.0
16	97.0
17	238.0
18	558.0
19	1174.0
20	2016.0
21	3300.0
22	5702.0
23	10873.0
24	23851.0
25	55849.0
26	97621.0
27	108636.0
28	90529.0
29	66635.0
30	48807.0
31	40514.0
32	38692.0
33	44691.0
34	70664.0
35	96608.0
36	106471.0
37	155946.0
38	299433.0
39	367546.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.663807026079343	15.452595699599556	18.67746420891114	39.20613306540996
2	54.50465363323095	16.726594369578798	17.45597544931383	11.312776547876428
3	20.781890990116967	15.999458779432466	51.14701335490539	12.07163687554518
4	18.82572409842154	18.10653408681984	50.434732542038155	12.63300927272047
5	17.05149944006702	20.812809654453698	49.57373122641157	12.56195967906772
6	17.637226762781296	23.357352395044952	48.59400684586441	10.411413996309337
7	55.86213845458497	3.9176884124100724	36.80967748436074	3.4104956486442135
8	57.59865040318053	2.896739146080613	36.39570132685404	3.1089091238848123
9	53.750687321332435	4.4130203850151	37.678566801875846	4.157725491776614
10	26.733129320048484	16.426850297815253	45.08298235563373	11.757038026502535
11	19.393487504426204	18.466042727636506	47.66345292964421	14.477016838293084
12	16.592613490786295	16.234026076467558	52.35606555678066	14.817294875965489
13	15.886493380123962	17.70971577283706	53.5919484804081	12.811842366630874
14	14.723560079801246	19.37765392399306	52.259624657778744	13.639161338426947
15	13.962857299136639	19.0108906245052	53.56321772900395	13.463034347354208
16	15.842504814847869	18.36280778321237	51.34611343177022	14.448573970169534
17	16.122269786937586	18.24028465898786	49.76494330138789	15.87250225268667
18	16.59422563715767	17.99690813356633	51.16371058518035	14.245155644095659
19	15.453056312848517	20.43407041049277	49.624571413766006	14.488301862892708
20	16.064174940911958	19.701925651263952	50.23960525444564	13.994294153378453
21	15.707660286213557	19.233194093786615	51.348704381295654	13.71044123870418
22	15.912345298722085	17.906455206800956	50.13308844062263	16.048111053854324
23	14.405276324766886	19.2440760817934	50.249738745922855	16.100908847516862
24	14.900090107466829	18.480667198291126	50.97359246667031	15.645650227571734
25	14.477650181510409	19.816042583694866	50.183295284759744	15.523011950034979
26	14.369060608067066	20.39405463448899	50.34036440265659	14.896520354787354
27	15.210428283956553	19.683098084712533	50.209032050045636	14.897441581285284
28	14.273771242187566	19.32376217386423	50.72883410150189	15.673632482446317
29	15.007988761036724	18.49177949292239	50.73626149014143	15.763970255899448
30	15.850277663424142	19.47996764191926	49.51557880372982	15.154175890926783
31	15.738003183989083	20.915123372379902	47.495674553708945	15.851198889922069
32	16.184107115611045	21.490371743680242	46.23393971148337	16.091581429225336
33	17.2193353926584	22.18952507895199	43.47924217605214	17.11189735233747
34	16.032968393294624	23.67316035386613	41.67985651897295	18.614014733866302
35	17.95148015188722	23.708685150692503	39.666170547813095	18.673664149607184
36	19.014517953840794	25.72375296159925	37.678624378531964	17.58310470602799
37	18.295673402175822	26.79266358247712	37.45275115657108	17.458911858775977
38	18.879155235301397	27.01416097857285	36.378658636642356	17.728025149483393
39	18.63721812628288	25.637215247450072	37.41970215595789	18.305864470309157
40	18.667273140777805	24.42810546891868	37.771610678166645	19.133010712136873
41	18.558165377429376	25.417099691101242	36.0561717857112	19.968563145758182
42	18.92504383022947	26.19202390582762	35.957427820464474	18.925504443478435
43	18.899767678192553	25.581423467669268	36.621344242190446	18.897464611947733
44	18.142174036958455	25.398905467767147	36.14938839197036	20.309532103304036
45	18.903682890808753	23.810768561994227	36.80236524903343	20.483183298163592
46	19.284207011109416	24.395229198273853	36.53486410469739	19.78569968591934
47	17.58316228268411	24.927295077483784	38.115516045174644	19.37402659465746
48	18.620981509256886	23.74962215319421	37.41290811053567	20.21648822701324
49	18.58200211306328	23.078911686046013	38.2339512268146	20.105134974076112
50	17.90058238787666	22.68836922758037	38.253354559927224	21.157693824615748
51	18.036175413040535	22.707714984036873	36.34296110984762	22.913148493074967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	382.0
1	676.0
2	970.0
3	63404.5
4	125839.0
5	90062.5
6	54286.0
7	52833.5
8	51381.0
9	51261.0
10	51141.0
11	50552.5
12	49964.0
13	47907.0
14	45850.0
15	43188.0
16	40526.0
17	37569.0
18	34612.0
19	31711.5
20	28811.0
21	26432.0
22	24053.0
23	22177.5
24	20302.0
25	18938.5
26	17852.5
27	18130.0
28	18363.0
29	18596.0
30	20201.0
31	21806.0
32	23969.5
33	26133.0
34	30920.0
35	35707.0
36	39598.0
37	43489.0
38	47827.0
39	52165.0
40	57468.0
41	62771.0
42	69531.0
43	76291.0
44	85056.5
45	93822.0
46	108644.0
47	123466.0
48	133848.0
49	144230.0
50	138739.0
51	133248.0
52	113461.0
53	93674.0
54	79832.0
55	65990.0
56	57576.5
57	49163.0
58	43820.5
59	38478.0
60	34089.5
61	29701.0
62	26518.0
63	23335.0
64	19510.5
65	15686.0
66	12661.5
67	9637.0
68	7887.5
69	6138.0
70	5055.0
71	3972.0
72	3146.0
73	2320.0
74	1962.0
75	1127.0
76	650.0
77	556.5
78	463.0
79	343.0
80	223.0
81	190.0
82	157.0
83	101.5
84	46.0
85	25.0
86	4.0
87	11.5
88	19.0
89	11.0
90	3.0
91	4.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1736815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.92123795962593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00060281615073	33.118030696097485
2	9.489736650434702	7.956430165941132
3	3.4355651875395306	4.3206943725795455
4	1.783469891261861	2.990610628216665
5	1.0226855938754154	2.1436123069366317
6	0.7340283489048175	1.8462826250130107
7	0.5463532072349845	1.603266196735182
8	0.42860507666452835	1.4374124327645923
9	0.34992206232012013	1.3202249437660327
>10	2.8928471351108946	25.348457360673066
>50	0.2554496438455092	6.787236923557419
>100	0.05598736858506819	4.537827398756653
>500	0.0019546545002342773	0.6277306987469999
>1k	0.0026527453931750903	2.257840506747251
>5k	0.0	0.0
>10k+	1.3961817858816264E-4	3.70434274346831
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63290	3.6440265658691335	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	3978	0.22903993804751802	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	3695	0.2127457443654045	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2956	0.1701965954923236	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	2639	0.15194479550211162	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTT	2374	0.13668698163016788	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	2348	0.13518998857103376	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	2232	0.12851109646105083	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2105	0.12119886113374193	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2053	0.11820487501547373	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	1979	0.11394420246255359	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1515331224108497E-4	0.0	0.0	0.16403589328742554	0.0
2	1.1515331224108497E-4	0.0	0.0	0.7394569945561271	0.0
3	1.7272996836162745E-4	0.0	0.0	1.2026036163897709	0.0
4	1.7272996836162745E-4	0.0	0.0	1.622049556227923	0.0
5	1.7272996836162745E-4	0.0	0.0	2.6185863203622723	0.0
6	1.7272996836162745E-4	0.0	0.0	3.337833908620089	0.0
7	1.7272996836162745E-4	0.0	0.0	3.9893137726240275	0.0
8	1.7272996836162745E-4	0.0	0.0	4.860160696447233	0.0
9	1.7272996836162745E-4	0.0	0.0	5.202281187115496	0.0
10	1.7272996836162745E-4	0.0	0.0	5.988605579753744	0.0
11	1.7272996836162745E-4	0.0	0.0	7.2074458131695085	0.0
12	1.7272996836162745E-4	0.0	0.0	8.074319947720396	0.0
13	2.3030662448216994E-4	0.0	0.0	8.442177203674543	0.0
14	2.3030662448216994E-4	0.0	0.0	8.583700624418835	0.0
15	2.3030662448216994E-4	0.0	0.0	8.831798435642254	0.0
16	2.3030662448216994E-4	0.0	0.0	9.434741178536575	0.0
17	2.3030662448216994E-4	0.0	0.0	10.206556253832446	0.0
18	2.3030662448216994E-4	0.0	0.0	11.041878380829276	0.0
19	2.3030662448216994E-4	0.0	0.0	11.56012586257028	0.0
20	2.3030662448216994E-4	0.0	0.0	12.026151317209951	0.0
21	2.3030662448216994E-4	0.0	0.0	12.692140498556265	0.0
22	2.3030662448216994E-4	0.0	0.0	13.355653883689397	0.0
23	2.3030662448216994E-4	0.0	0.0	14.08094702083987	0.0
24	2.3030662448216994E-4	0.0	0.0	14.630343473542087	0.0
25	2.3030662448216994E-4	0.0	0.0	15.14317874960776	0.0
26	2.8788328060271246E-4	0.0	0.0	15.622446835155154	0.0
27	2.8788328060271246E-4	0.0	0.0	16.09446026203136	0.0
28	2.8788328060271246E-4	0.0	0.0	16.601940909077822	0.0
29	2.8788328060271246E-4	0.0	0.0	17.117770171261764	0.0
30	2.8788328060271246E-4	0.0	0.0	17.68858514004082	0.0
31	2.8788328060271246E-4	0.0	0.0	18.22335712208842	0.0
32	2.8788328060271246E-4	0.0	0.0	18.743965246730365	0.0
33	2.8788328060271246E-4	0.0	0.0	19.247876141097354	0.0
34	2.8788328060271246E-4	0.0	0.0	19.708662120030056	0.0
35	2.8788328060271246E-4	0.0	0.0	20.207448692002316	0.0
36	2.8788328060271246E-4	0.0	0.0	20.68660162423747	0.0
37	2.8788328060271246E-4	0.0	0.0	21.188267029015755	0.0
38	2.8788328060271246E-4	0.0	0.0	21.689471820545076	0.0
39	2.8788328060271246E-4	0.0	0.0	22.188834159078542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.034283E-4	45.000004	2
CTATCGT	20	7.034283E-4	45.000004	2
CTGCGAT	20	7.034283E-4	45.000004	19
CGTAGCG	20	7.034283E-4	45.000004	2
CTACGAC	20	7.034283E-4	45.000004	25
TTGCGTC	20	7.034283E-4	45.000004	11
CGTTTCG	25	3.8916332E-5	45.0	39
CGTTTTT	55000	0.0	44.607273	1
ACCGGTA	75	0.0	42.0	41
GCCCTCG	340	0.0	41.02941	13
CGTGTAC	345	0.0	39.782608	18
CCGGTAT	85	0.0	39.705883	42
ACTAGCG	40	3.4595905E-7	39.375004	1
CCGCCCG	80	0.0	39.375004	29
CGCCCCG	80	0.0	39.375004	25
GTTTTTT	62905	0.0	39.033863	2
AGGGTCG	180	0.0	38.75	6
AAGTACG	35	6.250135E-6	38.571426	1
CGGTAAC	35	6.250135E-6	38.571426	23
TACGTCG	35	6.250135E-6	38.571426	1
>>END_MODULE