##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934922.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 988931 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.619885512740524 34.0 31.0 34.0 31.0 34.0 2 32.78422660428281 34.0 31.0 34.0 31.0 34.0 3 32.82563495329806 34.0 31.0 34.0 31.0 34.0 4 36.221086203183035 37.0 37.0 37.0 35.0 37.0 5 36.11369650663191 37.0 35.0 37.0 35.0 37.0 6 36.11155985604658 37.0 36.0 37.0 35.0 37.0 7 36.269482906289724 37.0 37.0 37.0 35.0 37.0 8 36.30528621309272 37.0 37.0 37.0 35.0 37.0 9 38.124908613442194 39.0 39.0 39.0 37.0 39.0 10 37.58891267439285 39.0 38.0 39.0 35.0 39.0 11 37.528538391455015 39.0 37.0 39.0 35.0 39.0 12 37.501773126739884 39.0 37.0 39.0 35.0 39.0 13 37.475627723268865 39.0 37.0 39.0 35.0 39.0 14 38.80600163206533 40.0 38.0 41.0 35.0 41.0 15 38.84929787821395 40.0 38.0 41.0 35.0 41.0 16 38.93498130809935 40.0 38.0 41.0 35.0 41.0 17 38.945989153945014 40.0 38.0 41.0 35.0 41.0 18 38.425959950694235 39.0 38.0 41.0 35.0 41.0 19 37.90126409223697 38.0 37.0 41.0 35.0 41.0 20 37.28348388310206 38.0 35.0 41.0 34.0 41.0 21 37.246734099750135 38.0 35.0 41.0 34.0 41.0 22 37.063576730833596 38.0 35.0 40.0 34.0 41.0 23 36.95502820722578 38.0 35.0 40.0 34.0 41.0 24 36.76527381586784 37.0 35.0 40.0 33.0 41.0 25 36.631958144703724 37.0 35.0 40.0 33.0 41.0 26 36.76850255477885 37.0 35.0 40.0 33.0 41.0 27 36.81921893438471 38.0 35.0 40.0 34.0 41.0 28 36.78571811380167 38.0 35.0 40.0 33.0 41.0 29 36.651826062687896 38.0 35.0 40.0 33.0 41.0 30 36.35010834931861 38.0 35.0 40.0 33.0 41.0 31 35.79064060081037 38.0 35.0 40.0 31.0 41.0 32 34.964928796852355 38.0 35.0 40.0 22.0 41.0 33 33.92871393454144 38.0 34.0 41.0 15.0 41.0 34 33.045314587165336 38.0 33.0 41.0 10.0 41.0 35 32.496615031786845 38.0 32.0 41.0 8.0 41.0 36 32.28181642601961 38.0 31.0 41.0 7.0 41.0 37 32.06380930519925 38.0 31.0 41.0 7.0 41.0 38 31.93719582053753 38.0 30.0 41.0 7.0 41.0 39 31.87686501889414 38.0 30.0 41.0 7.0 41.0 40 31.76577637873623 38.0 29.0 41.0 7.0 41.0 41 31.618914767562146 37.0 29.0 40.0 7.0 41.0 42 31.492905976251123 37.0 27.0 40.0 7.0 41.0 43 31.37223931699987 37.0 25.0 40.0 7.0 41.0 44 31.246027275917125 37.0 24.0 40.0 7.0 41.0 45 31.172658153096627 37.0 24.0 40.0 7.0 41.0 46 31.105846616194658 37.0 23.0 40.0 7.0 41.0 47 31.05700802179323 37.0 23.0 40.0 7.0 41.0 48 30.986367097401132 37.0 23.0 40.0 7.0 41.0 49 30.868538856603745 36.0 24.0 40.0 7.0 41.0 50 30.777574977425118 36.0 24.0 40.0 7.0 41.0 51 30.22738593491356 35.0 23.0 40.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 4.0 10 5.0 11 8.0 12 7.0 13 18.0 14 14.0 15 27.0 16 65.0 17 140.0 18 304.0 19 582.0 20 1020.0 21 1667.0 22 2961.0 23 5640.0 24 12263.0 25 27156.0 26 46531.0 27 53560.0 28 45698.0 29 33742.0 30 26022.0 31 22331.0 32 21915.0 33 26014.0 34 45831.0 35 66001.0 36 62246.0 37 81997.0 38 165169.0 39 239837.0 40 149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.359005835594193 15.311381683858633 19.51875307781837 36.81085940272881 2 51.30287148446151 17.047296525237858 18.754594607712775 12.895237382587865 3 21.94187461005874 18.323523076938635 47.075073994040025 12.659528318962595 4 20.53429410140849 19.239764958323686 46.99822333408499 13.227717606182837 5 18.214415363660358 21.63133727226672 46.194426102528894 13.95982126154403 6 18.908902643359344 24.723565142563032 45.469906393873785 10.897625820203837 7 58.85607792656919 4.280177282338202 33.340445390022154 3.523299401070449 8 60.34637401396053 3.2145822104878903 32.65141855195155 3.787625223600029 9 56.592522633024956 4.618623544008631 34.30168535519667 4.487168467769743 10 27.899722023073398 19.625838405308357 40.94562714688891 11.52881242472933 11 21.350023409115497 19.424611019373444 45.22327644699175 14.002089124519305 12 19.052492034327976 17.723885690710475 48.38386095693228 14.839761318029264 13 15.966331321396538 20.403546860195505 49.12263848539484 14.507483333013122 14 14.026054396110549 21.666324546404148 48.37850163459331 15.92911942289199 15 13.590836974470413 20.312539499722426 52.12011758150973 13.976505944297429 16 15.017731267398837 19.060581577481138 50.23980439484655 15.681882760273465 17 15.08811029283135 19.361006986331706 47.463473184681234 18.087409536155707 18 15.685826412560633 19.377792788374517 49.43165903384564 15.504721765219212 19 16.352000291223554 20.7932606015991 47.372769182076404 15.481969925100941 20 17.219704913689633 20.384738672364403 47.66621735995737 14.7293390539886 21 16.389111070438688 20.589808591297068 48.17181380703002 14.849266531234232 22 15.7906871156835 19.623512661651823 46.8027597476467 17.783040475017977 23 14.292200365849588 20.441769951594196 47.72446207066014 17.541567611896077 24 15.562157521606665 19.44079010567977 48.943050627394626 16.054001745318935 25 14.032121553475418 20.551585499898376 48.10649074606823 17.309802200557975 26 13.789738616748792 21.373280845680842 48.843549246610735 15.99343129095963 27 15.149489701505969 21.122403888643394 47.809806750926 15.918299658924637 28 12.988772725296304 20.12951358588213 49.141244434647106 17.740469254174457 29 14.199979573903537 19.774281522168888 49.04518110970331 16.980557794224268 30 16.41155955268871 20.378165918552458 47.00894197876293 16.201332549995907 31 15.675613364329767 21.39219015280136 44.58592156581197 18.346274917056903 32 16.09606736971538 22.672663714657546 44.14251348172926 17.088755433897816 33 17.610530967276787 22.970257783404506 41.96288719839908 17.45632405091963 34 15.744880077578719 23.05034426062081 41.00508528906466 20.199690372735812 35 16.58012540814273 24.408983033194428 39.346021107640475 19.664870451022367 36 19.589839938276786 24.30624583514927 37.565007063182364 18.53890716339158 37 17.355305880794514 25.974815229778418 37.93904731472671 18.730831574700357 38 17.067520383120762 26.941717875160148 36.41952775269458 19.57123398902451 39 18.399059186131286 25.904537323635317 36.83593698650361 18.860466503729782 40 19.68661109824649 24.000764461827973 36.907933920566755 19.404690519358784 41 17.455616215893727 24.620221228781382 36.30081370692192 21.623348848402973 42 18.211280665688506 24.490990776909612 36.89488953223228 20.4028390251696 43 19.636658169275716 24.90416419345738 35.59024846020602 19.86892917706089 44 18.25223397790139 25.569326879226157 34.621424548325415 21.55701459454704 45 18.406845371416207 25.478420638042493 34.29966296940838 21.81507102113292 46 19.648084648979555 24.183588137089444 35.85781009999687 20.310517113934136 47 16.898347811930257 24.271663038169496 37.84723099993832 20.982758149961928 48 17.649765251569622 23.163193387607425 37.56126564947403 21.625775711348922 49 18.37337488662 22.43614569671696 38.55445931010353 20.636020106559506 50 18.03634429500137 22.827376227461773 37.236571611163974 21.899707866372882 51 17.06974500748788 23.26997535722917 35.68044686636378 23.979832768919167 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 340.0 1 551.5 2 763.0 3 33413.0 4 66063.0 5 45422.0 6 24781.0 7 24608.0 8 24435.0 9 24889.0 10 25343.0 11 25512.0 12 25681.0 13 24864.5 14 24048.0 15 22608.5 16 21169.0 17 19503.0 18 17837.0 19 16463.0 20 15089.0 21 13776.5 22 12464.0 23 11461.0 24 10458.0 25 9814.0 26 9827.0 27 10484.0 28 10766.0 29 11048.0 30 12171.5 31 13295.0 32 14337.5 33 15380.0 34 18431.5 35 21483.0 36 23469.0 37 25455.0 38 27905.5 39 30356.0 40 35278.5 41 40201.0 42 44150.0 43 48099.0 44 53837.0 45 59575.0 46 66262.5 47 72950.0 48 78160.0 49 83370.0 50 80763.0 51 78156.0 52 66413.0 53 54670.0 54 47422.0 55 40174.0 56 34938.5 57 29703.0 58 26855.5 59 24008.0 60 21159.0 61 18310.0 62 15728.0 63 13146.0 64 11046.0 65 8946.0 66 7124.5 67 5303.0 68 4320.0 69 3337.0 70 2522.5 71 1708.0 72 1374.0 73 1040.0 74 807.0 75 426.5 76 279.0 77 205.5 78 132.0 79 89.0 80 46.0 81 40.0 82 34.0 83 21.5 84 9.0 85 9.5 86 10.0 87 5.5 88 1.0 89 3.0 90 5.0 91 3.5 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 988931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.4883234197005 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02855615365625 28.471311929068712 2 9.824360786174699 7.169489075155308 3 3.6748707815454593 4.0226962080791475 4 1.7923112161087804 2.615937252885356 5 1.0937492155026376 1.995453755765197 6 0.666322338645987 1.4587790996571581 7 0.4607894199016192 1.176940336722252 8 0.34903528214814344 1.0188569807926298 9 0.2661026786242516 0.8738676540441283 >10 3.215078953412276 28.88473965364528 >50 0.5653485560888062 13.205915563252132 >100 0.05867875815000407 3.632131884241637 >500 0.002821094141798088 0.689677869470452 >1k 0.0016926564850788528 1.1697760162182411 >5k 0.0 0.0 >10k+ 2.821094141798088E-4 3.6144267210023853 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35113 3.550601609212372 No Hit CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC 3140 0.3175145687616224 TruSeq Adapter, Index 13 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC 2998 0.30315562966475923 TruSeq Adapter, Index 13 (95% over 21bp) CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT 1677 0.16957704834816584 TruSeq Adapter, Index 19 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC 1398 0.14136476660151215 TruSeq Adapter, Index 13 (95% over 21bp) CGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG 1078 0.10900659398886273 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC 1073 0.10850099754179009 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0111928941452943E-4 0.0 0.0 0.1793856194213752 0.0 2 1.0111928941452943E-4 0.0 0.0 1.0829875896296102 0.0 3 1.0111928941452943E-4 0.0 0.0 1.5473273666211294 0.0 4 1.0111928941452943E-4 0.0 0.0 2.0103525928502597 0.0 5 1.0111928941452943E-4 0.0 0.0 3.170898677460814 0.0 6 1.0111928941452943E-4 0.0 0.0 3.761940924088738 0.0 7 1.0111928941452943E-4 0.0 0.0 4.406879751974607 0.0 8 1.0111928941452943E-4 0.0 0.0 5.401893559813576 0.0 9 1.0111928941452943E-4 0.0 0.0 5.735991692039182 0.0 10 1.0111928941452943E-4 0.0 0.0 6.522800882973635 0.0 11 1.0111928941452943E-4 0.0 0.0 7.740984962550471 0.0 12 1.0111928941452943E-4 0.0 0.0 8.66521526779927 0.0 13 1.0111928941452943E-4 0.0 0.0 9.020548450801927 0.0 14 1.0111928941452943E-4 0.0 0.0 9.152408004198472 0.0 15 1.0111928941452943E-4 0.0 0.0 9.434328583086181 0.0 16 1.0111928941452943E-4 0.0 0.0 10.079267410972049 0.0 17 1.0111928941452943E-4 0.0 0.0 10.871638162824302 0.0 18 1.0111928941452943E-4 0.0 0.0 11.795261752336614 0.0 19 1.0111928941452943E-4 0.0 0.0 12.32361003952753 0.0 20 1.0111928941452943E-4 0.0 0.0 12.811005014505563 0.0 21 1.0111928941452943E-4 0.0 0.0 13.432585286536675 0.0 22 1.0111928941452943E-4 0.0 0.0 14.113825939322359 0.0 23 2.0223857882905886E-4 0.0 0.0 14.790819581952634 0.0 24 2.0223857882905886E-4 0.0 0.0 15.3459644808384 0.0 25 2.0223857882905886E-4 0.0 0.0 15.805450531938021 0.0 26 2.0223857882905886E-4 0.0 0.0 16.228432519559 0.0 27 2.0223857882905886E-4 0.0 0.0 16.63189848432297 0.0 28 2.0223857882905886E-4 0.0 0.0 17.057711812047554 0.0 29 2.0223857882905886E-4 0.0 0.0 17.50658033775865 0.0 30 2.0223857882905886E-4 0.0 0.0 18.01955849295856 0.0 31 3.033578682435883E-4 0.0 0.0 18.509885927329613 0.0 32 3.033578682435883E-4 0.0 0.0 18.969675336297477 0.0 33 3.033578682435883E-4 0.0 0.0 19.41328565895902 0.0 34 3.033578682435883E-4 0.0 0.0 19.845975098363787 0.0 35 3.033578682435883E-4 0.0 0.0 20.30394436012219 0.0 36 3.033578682435883E-4 0.0 0.0 20.7278364213479 0.0 37 3.033578682435883E-4 0.0 0.0 21.16719973385403 0.0 38 3.033578682435883E-4 0.0 0.0 21.602821632651825 0.0 39 3.033578682435883E-4 0.0 0.0 22.035915549214252 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGCG 25 3.8904087E-5 45.000004 1 TTGTGCG 60 0.0 45.000004 1 CGTTCGG 25 3.8904087E-5 45.000004 2 ACGATAG 30 2.1651922E-6 45.000004 1 GTTCGTA 25 3.8904087E-5 45.000004 10 ACGTCTA 25 3.8904087E-5 45.000004 28 ATCGCAC 25 3.8904087E-5 45.000004 12 ACCGGTG 20 7.03281E-4 45.0 15 GCGCGTG 35 1.2117016E-7 45.0 1 CGGCCAC 20 7.03281E-4 45.0 41 CTGCGTA 20 7.03281E-4 45.0 2 TACGCCG 20 7.03281E-4 45.0 1 GTATGCG 55 1.8189894E-12 45.0 1 GAACGAT 20 7.03281E-4 45.0 44 CTCGCGA 20 7.03281E-4 45.0 20 ACGAGGC 20 7.03281E-4 45.0 18 CGTTTTT 28015 0.0 44.67071 1 CGGGAAC 210 0.0 42.857143 6 ACGGGTC 105 0.0 40.714287 5 GCGTAAG 95 0.0 40.263157 1 >>END_MODULE