##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934921.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1245065
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65029054707987	34.0	31.0	34.0	31.0	34.0
2	32.80394356921125	34.0	31.0	34.0	31.0	34.0
3	32.84459445892383	34.0	31.0	34.0	31.0	34.0
4	36.23232521996844	37.0	37.0	37.0	35.0	37.0
5	36.1234594177814	37.0	37.0	37.0	35.0	37.0
6	36.11764767301305	37.0	36.0	37.0	35.0	37.0
7	36.25232497901716	37.0	37.0	37.0	35.0	37.0
8	36.24995321529398	37.0	37.0	37.0	35.0	37.0
9	37.99517294277809	39.0	39.0	39.0	35.0	39.0
10	37.63761008461405	39.0	38.0	39.0	35.0	39.0
11	37.539057800195174	39.0	37.0	39.0	35.0	39.0
12	37.45251131467032	39.0	37.0	39.0	35.0	39.0
13	37.44247890672374	39.0	37.0	39.0	35.0	39.0
14	38.757639962572235	40.0	38.0	41.0	35.0	41.0
15	38.79202370960552	40.0	38.0	41.0	35.0	41.0
16	38.86970800721247	40.0	38.0	41.0	35.0	41.0
17	38.874707746181926	40.0	38.0	41.0	35.0	41.0
18	38.33045905233863	39.0	38.0	41.0	35.0	41.0
19	37.81800548565737	38.0	37.0	41.0	35.0	41.0
20	37.24910586997466	38.0	35.0	41.0	34.0	41.0
21	37.18128933027593	38.0	35.0	41.0	34.0	41.0
22	36.9888592161855	38.0	35.0	40.0	34.0	41.0
23	36.89559259958315	38.0	35.0	40.0	33.0	41.0
24	36.68543810965693	37.0	35.0	40.0	33.0	41.0
25	36.54866211804203	37.0	35.0	40.0	33.0	41.0
26	36.66920763173007	37.0	35.0	40.0	33.0	41.0
27	36.68631677864208	37.0	35.0	40.0	33.0	41.0
28	36.579630782328636	37.0	35.0	40.0	33.0	41.0
29	36.43881564416316	37.0	35.0	40.0	33.0	41.0
30	36.12437663897066	37.0	35.0	40.0	32.0	41.0
31	35.55891620116219	37.0	35.0	40.0	30.0	41.0
32	34.81328364382583	37.0	35.0	40.0	22.0	41.0
33	33.82246629694032	37.0	34.0	40.0	16.0	41.0
34	32.97988619068081	37.0	33.0	40.0	10.0	41.0
35	32.487207495191015	37.0	32.0	40.0	10.0	41.0
36	32.24116170641693	37.0	31.0	40.0	8.0	41.0
37	31.988424700718436	37.0	30.0	40.0	8.0	41.0
38	31.832211972868887	37.0	30.0	40.0	7.0	41.0
39	31.735698939412803	37.0	29.0	40.0	7.0	41.0
40	31.597319015473087	36.0	29.0	40.0	7.0	41.0
41	31.44832438467068	36.0	28.0	40.0	7.0	41.0
42	31.311282543481667	36.0	27.0	40.0	7.0	41.0
43	31.19068803636758	36.0	25.0	40.0	7.0	41.0
44	31.075331006814906	36.0	24.0	40.0	7.0	41.0
45	31.033066546726477	35.0	24.0	40.0	7.0	41.0
46	31.00114612490111	35.0	24.0	40.0	7.0	41.0
47	30.991709669776277	35.0	24.0	40.0	7.0	41.0
48	30.948766530261473	35.0	24.0	40.0	7.0	41.0
49	30.825723958186924	35.0	24.0	40.0	7.0	41.0
50	30.72388429519744	35.0	24.0	40.0	7.0	41.0
51	30.17130671892632	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	16.0
10	10.0
11	15.0
12	21.0
13	25.0
14	38.0
15	76.0
16	131.0
17	284.0
18	521.0
19	994.0
20	1621.0
21	2561.0
22	4007.0
23	6670.0
24	12640.0
25	27779.0
26	50676.0
27	65860.0
28	62290.0
29	49479.0
30	38984.0
31	32772.0
32	31453.0
33	36849.0
34	64107.0
35	82826.0
36	86209.0
37	111018.0
38	199720.0
39	275230.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.738419279314737	15.027408207603617	18.100982679619136	38.13318983346251
2	52.26048439238112	17.294759711340372	17.553220113006148	12.89153578327236
3	23.639729652668738	17.461337359896874	46.300875857886936	12.598057129547454
4	21.35350363234048	18.49252850252798	46.43797713372394	13.715990731407599
5	19.16229273170477	21.114881552368754	45.12760377972234	14.595221936204133
6	20.530574708950937	23.831366233891405	44.31937288414661	11.318686173011047
7	58.45646612827443	4.476071530402027	33.40676992767445	3.6606924136490866
8	58.768578347315206	4.072076558251979	33.393196339146954	3.7661487552858683
9	53.5871621160341	6.031331697541895	35.32699096031131	5.054515226112692
10	29.986868155477826	19.68708461004044	39.82820174047138	10.497845494010354
11	23.688883712898523	17.89922614481975	44.65003835141137	13.761851790870356
12	21.853236578009984	16.13200917221189	47.5622557858425	14.452498463935617
13	18.71460526157269	17.53016910763695	48.311774887254884	15.443450743535477
14	15.323617642452403	20.22006883174774	48.060141438398794	16.39617208740106
15	14.439567412143143	18.650190953885943	52.016481067253515	14.8937605667174
16	16.993811568070743	17.4903318300651	50.17770156578171	15.338155036082455
17	17.093485079092254	17.767104528679226	46.96863215976676	18.17077823246176
18	16.96835104994518	18.93901121628188	47.92464650439937	16.167991229373566
19	18.165316670214004	19.015071502291043	47.064771718745604	15.754840108749343
20	19.782983217743652	18.706493235292935	46.58800946135342	14.92251408560999
21	18.45759056756073	19.018043234690555	47.515832506736594	15.008533691012115
22	17.43804540325204	18.685128888853193	46.91771112351564	16.95911458437913
23	17.368490801685052	18.815885114431776	47.83798436226221	15.977639721620958
24	17.505913345889574	17.177255805921778	48.655130455036485	16.661700393152167
25	16.3325609506331	18.96077714818102	47.05151939858562	17.655142502600267
26	15.983984771879381	20.031644934200223	46.729688811427515	17.25468148249288
27	16.696638328119416	19.783465120294924	47.41053679928357	16.109359752302087
28	15.096802174986848	19.616887471738423	48.576419704995324	16.70989064827941
29	16.958070462184704	18.596378502327187	47.20685265427909	17.238698381209012
30	18.119054025291852	18.72103062892299	46.15044194479807	17.009473400987098
31	17.696746756193452	20.417488243585677	44.59108560597238	17.294679394248494
32	19.19096593350548	21.072715079132415	43.330830117303115	16.405488870058992
33	19.36782416982246	23.095260086822776	40.96669651785248	16.570219225502285
34	17.890632215988724	24.036737037825333	39.834948376189196	18.237682369996747
35	19.159642267672773	24.38306433800645	37.450815820860754	19.00647757346002
36	19.709814347042123	26.888716653347416	35.51147932035677	17.88998967925369
37	19.936710131599554	27.17376201242506	36.37528964351259	16.514238212462804
38	19.7049953215294	26.636199716480665	35.19149602631188	18.467308935678055
39	20.353877106817716	26.178392292771864	34.95247236088076	18.515258239529665
40	20.098388437551453	25.829093260191232	35.7946773863212	18.277840915936117
41	17.65731106408099	27.538803195013916	34.58044359129845	20.223442149606647
42	18.568187203077752	27.01858939091533	34.53787553260271	19.875347873404202
43	20.15123708400766	26.36866348343259	34.09187472140009	19.38822471115966
44	19.390232638456627	26.350752771943633	34.0642456417938	20.194768947805937
45	19.355937240224407	25.236513756309915	33.92545770702735	21.482091296438337
46	19.110407890351105	26.120082084067903	35.189648733198666	19.579861292382326
47	17.971591844602493	25.70090718155277	37.16906346255015	19.15843751129459
48	18.339604759590863	24.00693939673832	37.29925746848558	20.35419837518523
49	18.401448920337494	23.700288739945304	38.04877656989796	19.849485769819246
50	18.03158871223591	23.473152004112237	37.49884544180424	20.996413841847613
51	17.19749571307522	24.140908305992056	36.26372920289302	22.397866778039702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	722.5
2	1144.0
3	52718.0
4	104292.0
5	72676.0
6	41060.0
7	39117.0
8	37174.0
9	35392.0
10	33610.0
11	31710.5
12	29811.0
13	27965.5
14	26120.0
15	23905.0
16	21690.0
17	19852.0
18	18014.0
19	16471.0
20	14928.0
21	13731.0
22	12534.0
23	11538.0
24	10542.0
25	10651.5
26	11383.5
27	12006.0
28	12696.0
29	13386.0
30	14677.0
31	15968.0
32	17647.5
33	19327.0
34	21236.0
35	23145.0
36	24530.0
37	25915.0
38	29248.5
39	32582.0
40	35614.5
41	38647.0
42	43042.0
43	47437.0
44	52220.5
45	57004.0
46	64094.0
47	71184.0
48	76428.5
49	81673.0
50	81180.5
51	80688.0
52	71116.0
53	61544.0
54	56900.5
55	52257.0
56	50808.5
57	49360.0
58	48177.0
59	46994.0
60	45839.5
61	44685.0
62	40176.5
63	35668.0
64	31116.5
65	26565.0
66	21610.5
67	16656.0
68	14035.0
69	11414.0
70	9337.5
71	7261.0
72	6244.5
73	5228.0
74	4023.0
75	2402.0
76	1986.0
77	1348.0
78	710.0
79	591.0
80	472.0
81	356.0
82	240.0
83	236.0
84	232.0
85	124.5
86	17.0
87	13.0
88	9.0
89	5.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1245065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.388485473590165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9674544445385	26.366037056078675
2	9.024155879101144	6.026057949615638
3	3.163416752795114	3.1686508289283433
4	1.552246122717808	2.0730858847920075
5	0.8621212265715406	1.4392461024928809
6	0.6126772439552782	1.227381915588004
7	0.4333766827006485	1.012885375246031
8	0.35187101819816796	0.939875230374953
9	0.26567108967965536	0.7983319786669841
>10	4.0826427775827705	34.45284693556949
>50	0.5991902659543344	12.324276211930117
>100	0.07979177518445534	4.586547151439932
>500	0.003426640649441945	0.7868162631539545
>1k	0.0017133203247209725	0.9184698776056601
>5k	0.0	0.0
>10k+	2.4476004638871037E-4	3.8794912385172964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47472	3.8128129856674153	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2607	0.20938665852786803	RNA PCR Primer, Index 30 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2453	0.19701782637854248	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1462	0.11742358832671387	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	1372	0.1101950500576275	RNA PCR Primer, Index 30 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4095127563621176E-4	0.0	0.0	0.1175039054185926	0.0
2	2.4095127563621176E-4	0.0	0.0	0.6991602848044078	0.0
3	3.21268367514949E-4	0.0	0.0	1.0220349941569316	0.0
4	3.21268367514949E-4	0.0	0.0	1.335512603759643	0.0
5	3.21268367514949E-4	0.0	0.0	2.177637312108203	0.0
6	3.21268367514949E-4	0.0	0.0	2.6213089276463477	0.0
7	3.21268367514949E-4	0.0	0.0	3.0807226931927247	0.0
8	4.0158545939368627E-4	0.0	0.0	3.7078385465819053	0.0
9	4.0158545939368627E-4	0.0	0.0	3.9295940372590987	0.0
10	4.0158545939368627E-4	0.0	0.0	4.455269403605434	0.0
11	4.819025512724235E-4	0.0	0.0	5.271772959644677	0.0
12	4.819025512724235E-4	0.0	0.0	5.945954628874798	0.0
13	4.819025512724235E-4	0.0	0.0	6.205539469826877	0.0
14	4.819025512724235E-4	0.0	0.0	6.315493568608868	0.0
15	5.622196431511608E-4	0.0	0.0	6.537168742194183	0.0
16	5.622196431511608E-4	0.0	0.0	6.987988578909535	0.0
17	5.622196431511608E-4	0.0	0.0	7.517760116941686	0.0
18	5.622196431511608E-4	0.0	0.0	8.084557834329935	0.0
19	5.622196431511608E-4	0.0	0.0	8.442531112833466	0.0
20	5.622196431511608E-4	0.0	0.0	8.791187608679065	0.0
21	5.622196431511608E-4	0.0	0.0	9.219197391300856	0.0
22	5.622196431511608E-4	0.0	0.0	9.712906555079453	0.0
23	5.622196431511608E-4	0.0	0.0	10.14894804688912	0.0
24	5.622196431511608E-4	0.0	0.0	10.513427009834828	0.0
25	5.622196431511608E-4	0.0	0.0	10.841602647251348	0.0
26	5.622196431511608E-4	0.0	0.0	11.155642476497212	0.0
27	5.622196431511608E-4	0.0	0.0	11.45530554629678	0.0
28	5.622196431511608E-4	0.0	0.0	11.755771787015135	0.0
29	5.622196431511608E-4	0.0	0.0	12.067402103504635	0.0
30	5.622196431511608E-4	0.0	0.0	12.416942087360901	0.0
31	6.42536735029898E-4	0.0	0.0	12.768650632697891	0.0
32	6.42536735029898E-4	0.0	0.0	13.097629441033199	0.0
33	6.42536735029898E-4	0.0	0.0	13.409982611349609	0.0
34	6.42536735029898E-4	0.0	0.0	13.727476075546257	0.0
35	6.42536735029898E-4	0.0	0.0	14.06183612903744	0.0
36	6.42536735029898E-4	0.0	0.0	14.399730134571287	0.0
37	6.42536735029898E-4	0.0	0.0	14.72011501407557	0.0
38	6.42536735029898E-4	0.0	0.0	15.042507820876821	0.0
39	6.42536735029898E-4	0.0	0.0	15.383453875902061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTC	25	3.890993E-5	45.000004	12
CGCCTAT	20	7.033512E-4	45.000004	38
AGACGTC	20	7.033512E-4	45.000004	16
CTACCGT	25	3.890993E-5	45.000004	19
TTACGTG	20	7.033512E-4	45.000004	23
ATCGCGA	25	3.890993E-5	45.000004	12
GCGTACA	20	7.033512E-4	45.000004	1
TCGAACC	20	7.033512E-4	45.000004	14
ACGATAG	35	1.212029E-7	45.0	1
CGTTTTT	35305	0.0	44.458294	1
CGATAGA	60	3.6379788E-12	41.250004	10
GTTTTTT	39410	0.0	40.152878	2
TTTAGCG	40	3.4585355E-7	39.375004	1
CGATCGC	40	3.4585355E-7	39.375004	10
CCGTTCG	35	6.248709E-6	38.571426	5
CACAACG	65	9.094947E-12	38.076927	12
GGCTACG	65	9.094947E-12	38.076927	1
CGCATGG	125	0.0	37.8	2
CCTACCG	30	1.13997645E-4	37.500004	18
TTATCGG	30	1.13997645E-4	37.500004	2
>>END_MODULE