##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934919.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457749
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67944687322715	34.0	31.0	34.0	31.0	34.0
2	32.82771656849019	34.0	31.0	34.0	31.0	34.0
3	32.87750977706038	34.0	31.0	34.0	31.0	34.0
4	36.24949494048701	37.0	37.0	37.0	35.0	37.0
5	36.153533976013705	37.0	37.0	37.0	35.0	37.0
6	36.14844599447505	37.0	36.0	37.0	35.0	37.0
7	36.30461211086408	37.0	37.0	37.0	35.0	37.0
8	36.319596171906134	37.0	37.0	37.0	35.0	37.0
9	38.10721736046466	39.0	39.0	39.0	37.0	39.0
10	37.728418266793526	39.0	38.0	39.0	35.0	39.0
11	37.57121150486126	39.0	37.0	39.0	35.0	39.0
12	37.51206826415247	39.0	37.0	39.0	35.0	39.0
13	37.50816567186806	39.0	37.0	39.0	35.0	39.0
14	38.8257621853968	40.0	38.0	41.0	35.0	41.0
15	38.858577162460755	40.0	38.0	41.0	35.0	41.0
16	38.91251374550763	40.0	38.0	41.0	35.0	41.0
17	38.88216146949852	40.0	38.0	41.0	35.0	41.0
18	38.365658285479874	39.0	38.0	41.0	35.0	41.0
19	37.86825303944644	38.0	37.0	41.0	35.0	41.0
20	37.30668242612411	38.0	35.0	41.0	34.0	41.0
21	37.26175836855316	38.0	35.0	41.0	34.0	41.0
22	37.08258349002469	38.0	35.0	41.0	34.0	41.0
23	36.99276555840546	38.0	35.0	40.0	34.0	41.0
24	36.82626638742335	37.0	35.0	40.0	33.0	41.0
25	36.70207491138735	37.0	35.0	40.0	33.0	41.0
26	36.830456409162345	38.0	35.0	40.0	34.0	41.0
27	36.860115836128166	38.0	35.0	40.0	34.0	41.0
28	36.79048793722376	38.0	35.0	40.0	33.0	41.0
29	36.65332029039293	38.0	35.0	40.0	33.0	41.0
30	36.34929127030785	38.0	35.0	40.0	33.0	41.0
31	35.822969523559955	38.0	35.0	40.0	31.0	41.0
32	35.06904823807116	38.0	35.0	41.0	23.0	41.0
33	34.11354149445481	38.0	34.0	41.0	17.0	41.0
34	33.2838170357174	38.0	33.0	41.0	12.0	41.0
35	32.78347507012524	38.0	33.0	41.0	10.0	41.0
36	32.54466852661192	38.0	32.0	41.0	8.0	41.0
37	32.3188827431883	37.0	31.0	41.0	8.0	41.0
38	32.19173259594073	37.0	31.0	41.0	8.0	41.0
39	32.15200970811848	37.0	31.0	41.0	8.0	41.0
40	32.0580580058707	37.0	30.0	41.0	8.0	41.0
41	31.92448700016258	37.0	30.0	40.0	7.0	41.0
42	31.784495135993918	37.0	29.0	40.0	7.0	41.0
43	31.68347980345039	37.0	29.0	40.0	7.0	41.0
44	31.58805391051546	37.0	28.0	40.0	7.0	41.0
45	31.5743313835235	37.0	27.0	40.0	7.0	41.0
46	31.532161572396895	36.0	27.0	40.0	7.0	41.0
47	31.51515315736797	36.0	27.0	40.0	7.0	41.0
48	31.453517718070806	36.0	27.0	40.0	7.0	41.0
49	31.3416445492331	36.0	27.0	40.0	7.0	41.0
50	31.26325999880638	36.0	27.0	40.0	7.0	41.0
51	30.738600403773216	35.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	14.0
10	14.0
11	12.0
12	20.0
13	31.0
14	29.0
15	55.0
16	115.0
17	220.0
18	470.0
19	834.0
20	1521.0
21	2435.0
22	3928.0
23	6725.0
24	13572.0
25	30013.0
26	55234.0
27	72853.0
28	68868.0
29	56501.0
30	45003.0
31	38557.0
32	35645.0
33	41902.0
34	78150.0
35	98397.0
36	92518.0
37	117836.0
38	233131.0
39	362909.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.807565637157012	15.596923750247813	18.454617358681087	37.14089325391408
2	49.76028109091482	18.464084008975483	19.36122062165709	12.4144142784526
3	23.070226767433898	18.125205368002312	46.752012863668575	12.052555000895214
4	20.93316476293244	19.215722322567192	46.580172581150805	13.270940333349568
5	19.422410853994755	21.568116321808485	45.14940500730921	13.860067816887545
6	19.990066877082405	24.834865261440754	43.63117381661726	11.543894044859575
7	59.83327719655441	4.211561798361721	32.60657355964573	3.3485874454381377
8	61.05570986500419	3.8558764231702436	31.76699143679742	3.321422275028143
9	56.41636523160023	5.924819704901187	33.173474994666435	4.485340068832151
10	30.014906544267912	23.17298794236868	37.84444372796688	8.967661785396526
11	22.41586171556283	17.464392018104625	46.6447927592473	13.474953507085239
12	20.37518118688471	16.162453206964987	49.17286858025627	14.289497025894033
13	18.154291308037255	17.718825394495212	49.67144549576093	14.455437801706603
14	15.044976878735639	20.21829546787547	48.793242183668106	15.94348546972078
15	15.354289387267631	19.123319583824102	51.61382377899076	13.90856724991751
16	17.66723901028229	18.467719751479848	49.08663974387909	14.778401494358768
17	16.726885081039327	18.575077053731473	48.06849464482569	16.629543220403512
18	17.569211160494707	18.893513217981972	48.386793611245835	15.15048201027749
19	17.818979810653275	20.241619099035567	47.083757217463365	14.855643872847796
20	19.39119834758933	20.146060810194346	47.44637108308769	13.01636975912863
21	18.577478015762658	20.1627303465823	47.38977697806687	13.870014659588174
22	16.81969941327348	19.015825083742126	47.88458095323681	16.27989454974759
23	16.44501213857804	19.09032350562408	48.13654476868103	16.32811958711685
24	18.053793897303308	17.61359465861407	48.492092946042156	15.84051849804047
25	15.950962751475048	19.380153922245874	47.05130993058476	17.61757339569432
26	14.94880119965783	20.547364463978365	48.11219215379328	16.391642182570525
27	15.547326734575021	20.44453469012841	48.156712849742995	15.851425725553575
28	14.042335134512182	21.198368169005775	48.36559654645621	16.39370015002583
29	16.733882170387357	19.510972053487947	47.533080111871115	16.222065664253584
30	18.320575078425712	19.658253924372442	46.77019157619041	15.250979421011438
31	18.29649685919867	21.17566192808227	44.643625205711	15.884216007008064
32	19.549661841647637	22.08823329667865	43.375780055414204	14.986324806259512
33	20.02573831297432	23.229513448474325	40.80208595581269	15.942662282738663
34	17.903287877405507	24.15779396864618	41.26670640830486	16.67221174564345
35	18.245596464137517	25.719448272645018	38.48275663368659	17.55219862953087
36	19.249678785579686	28.532895580789287	36.46341036762845	15.754015266002583
37	19.923182934785068	28.465462847170535	36.07129896847811	15.540055249566285
38	19.151993930367986	28.65836299664757	34.17975248139426	18.009890591590185
39	20.321811230877195	26.79507926261654	35.05905337612991	17.824056130376352
40	20.32846532564934	25.82111186493697	35.70477496468871	18.145647844724984
41	17.734877540646572	27.474551517442304	34.63483768467685	20.15573325723427
42	17.6548226066353	27.895405862051696	34.855726191546005	19.594045339766996
43	19.13944032889064	26.345138977972205	34.908890350807994	19.60653034232917
44	18.202241949745808	26.0373356455741	35.795325532722025	19.965096871958067
45	19.628070401694668	24.923495059849124	35.24337866120985	20.20505587724636
46	19.82213673272971	25.703533324324006	35.86611961318444	18.608210329761846
47	17.667993598349234	24.897427472081958	38.66001623050333	18.774562699065477
48	18.104831490194815	24.097632720036167	38.41779346101421	19.379742328754812
49	18.550107048607135	23.67547499603841	38.93297131399164	18.841446641362815
50	18.08315423299896	23.21840042421569	38.38109304139464	20.317352301390706
51	17.299686022765236	23.05582099524678	37.3007973251911	22.343695656796882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	447.0
1	852.0
2	1257.0
3	64363.5
4	127470.0
5	86800.5
6	46131.0
7	42698.0
8	39265.0
9	37880.5
10	36496.0
11	34950.0
12	33404.0
13	31429.5
14	29455.0
15	26689.5
16	23924.0
17	21885.0
18	19846.0
19	18299.0
20	16752.0
21	14946.5
22	13141.0
23	13090.5
24	13040.0
25	13642.5
26	15625.0
27	17005.0
28	17633.5
29	18262.0
30	21014.5
31	23767.0
32	25111.0
33	26455.0
34	31133.0
35	35811.0
36	36598.0
37	37385.0
38	39381.5
39	41378.0
40	46666.5
41	51955.0
42	57961.5
43	63968.0
44	68661.0
45	73354.0
46	82105.5
47	90857.0
48	96710.5
49	102564.0
50	99916.5
51	97269.0
52	85944.0
53	74619.0
54	66495.5
55	58372.0
56	54564.0
57	50756.0
58	47955.0
59	45154.0
60	43567.5
61	41981.0
62	38776.0
63	35571.0
64	29024.5
65	22478.0
66	18147.5
67	13817.0
68	11018.0
69	8219.0
70	6465.5
71	4712.0
72	3837.5
73	2963.0
74	2373.5
75	1523.5
76	1263.0
77	880.0
78	497.0
79	453.0
80	409.0
81	304.0
82	199.0
83	110.0
84	21.0
85	17.5
86	14.0
87	11.5
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1457749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.855598480946227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37685636085118	24.64886067949125
2	9.813243402453269	6.252134832486919
3	3.5924037868319547	3.4331451784424845
4	1.7686686194359738	2.2536798954640744
5	1.027432524455774	1.636473898266405
6	0.6551375521315633	1.2521879286295836
7	0.44459523553449776	0.9913993116810075
8	0.35867437186565937	0.9140629420446432
9	0.29049692460287746	0.8328558051089076
>10	3.794037645597844	30.095170458198673
>50	0.7898061086988706	16.043114347695695
>100	0.08140646161179821	4.998404970321083
>500	0.003949639597509693	0.9185422710506868
>1k	0.003071941909174206	1.6094935190025959
>5k	0.0	0.0
>10k+	2.1942442208387183E-4	4.120473962116043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58949	4.043837450754554	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	3778	0.2591667015377819	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	3749	0.257177332997656	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	1902	0.13047513666618876	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1573	0.10790609357303622	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.057967455302662E-4	0.0	0.0	0.1385698086570459	0.0
2	2.057967455302662E-4	0.0	0.0	0.8382787434599509	0.0
3	2.057967455302662E-4	0.0	0.0	1.1706404874913308	0.0
4	2.057967455302662E-4	0.0	0.0	1.5069123696877857	0.0
5	2.057967455302662E-4	0.0	0.0	2.4557039654974897	0.0
6	2.057967455302662E-4	0.0	0.0	2.8991273532000363	0.0
7	2.057967455302662E-4	0.0	0.0	3.41238443655252	0.0
8	2.057967455302662E-4	0.0	0.0	4.159769617403271	0.0
9	2.057967455302662E-4	0.0	0.0	4.430803931266631	0.0
10	2.057967455302662E-4	0.0	0.0	5.137647153247919	0.0
11	2.057967455302662E-4	0.0	0.0	6.0638697059644695	0.0
12	2.7439566070702157E-4	0.0	0.0	6.935693318945854	0.0
13	2.7439566070702157E-4	0.0	0.0	7.229982665054135	0.0
14	2.7439566070702157E-4	0.0	0.0	7.345023045805553	0.0
15	2.7439566070702157E-4	0.0	0.0	7.57935694004935	0.0
16	2.7439566070702157E-4	0.0	0.0	8.048367723112827	0.0
17	2.7439566070702157E-4	0.0	0.0	8.680163731890744	0.0
18	2.7439566070702157E-4	0.0	0.0	9.371503599042084	0.0
19	2.7439566070702157E-4	0.0	0.0	9.833174298181648	0.0
20	3.42994575883777E-4	0.0	0.0	10.259036363598947	0.0
21	3.42994575883777E-4	0.0	0.0	10.724617200903586	0.0
22	3.42994575883777E-4	0.0	0.0	11.26407906985359	0.0
23	3.42994575883777E-4	0.0	0.0	11.783715852317512	0.0
24	3.42994575883777E-4	0.0	0.0	12.224944074734402	0.0
25	3.42994575883777E-4	0.0	0.0	12.603129894103855	0.0
26	3.42994575883777E-4	0.0	0.0	12.943654909041268	0.0
27	3.42994575883777E-4	0.0	0.0	13.286512287094691	0.0
28	3.42994575883777E-4	0.0	0.0	13.63458318270155	0.0
29	3.42994575883777E-4	0.0	0.0	13.998020235308	0.0
30	3.42994575883777E-4	0.0	0.0	14.407006967591814	0.0
31	3.42994575883777E-4	0.0	0.0	14.780185066153363	0.0
32	4.115934910605324E-4	0.0	0.0	15.16612256293779	0.0
33	4.115934910605324E-4	0.0	0.0	15.546777943253606	0.0
34	4.115934910605324E-4	0.0	0.0	15.922082608185635	0.0
35	4.801924062372878E-4	0.0	0.0	16.30609933534511	0.0
36	4.801924062372878E-4	0.0	0.0	16.69402620066966	0.0
37	4.801924062372878E-4	0.0	0.0	17.081953065994213	0.0
38	5.487913214140431E-4	0.0	0.0	17.489705017804848	0.0
39	5.487913214140431E-4	0.0	0.0	17.911588346141894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGC	25	3.8913226E-5	45.0	24
TATCTCG	20	7.0339104E-4	45.0	41
GTAGACG	25	3.8913226E-5	45.0	1
ACTACGT	25	3.8913226E-5	45.0	18
CACAACG	20	7.0339104E-4	45.0	12
ACGAACT	20	7.0339104E-4	45.0	22
CGTTTTT	40045	0.0	44.35385	1
ACGTTAG	70	0.0	41.785713	1
ATCGCTA	60	3.6379788E-12	41.250004	19
TCAATCG	55	6.184564E-11	40.909092	16
TCGACGT	55	6.184564E-11	40.909092	26
CGACGGT	50	1.0822987E-9	40.5	28
GTTTTTT	44820	0.0	39.83936	2
GAATGCG	80	0.0	39.375	1
TGCGCGA	35	6.2494437E-6	38.571426	15
ACGGATA	35	6.2494437E-6	38.571426	20
TGCAACG	70	0.0	38.571426	1
TACGTAG	100	0.0	38.25	1
ACGTAGG	165	0.0	38.18182	2
TATTGCG	65	9.094947E-12	38.076927	1
>>END_MODULE