##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934918.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1395723
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73148898456212	34.0	31.0	34.0	31.0	34.0
2	32.87638306454791	34.0	31.0	34.0	31.0	34.0
3	32.916922627197515	34.0	33.0	34.0	31.0	34.0
4	36.28322596962291	37.0	37.0	37.0	35.0	37.0
5	36.182469587446796	37.0	37.0	37.0	35.0	37.0
6	36.185466600464416	37.0	37.0	37.0	35.0	37.0
7	36.317663318581126	37.0	37.0	37.0	35.0	37.0
8	36.345392316383695	37.0	37.0	37.0	35.0	37.0
9	38.126790917682094	39.0	39.0	39.0	37.0	39.0
10	37.76302389514252	39.0	38.0	39.0	35.0	39.0
11	37.58749551307817	39.0	37.0	39.0	35.0	39.0
12	37.60572979022342	39.0	37.0	39.0	35.0	39.0
13	37.636152732311494	39.0	37.0	39.0	35.0	39.0
14	39.020137233534165	40.0	38.0	41.0	35.0	41.0
15	39.066654343304506	41.0	39.0	41.0	35.0	41.0
16	39.12013200326999	41.0	39.0	41.0	35.0	41.0
17	39.12882785481073	41.0	39.0	41.0	35.0	41.0
18	38.58438887945531	39.0	38.0	41.0	35.0	41.0
19	38.05459750967778	39.0	37.0	41.0	35.0	41.0
20	37.467426559568054	38.0	35.0	41.0	35.0	41.0
21	37.41730629931584	38.0	35.0	41.0	35.0	41.0
22	37.22957134044506	38.0	35.0	41.0	34.0	41.0
23	37.13025077325515	38.0	35.0	41.0	34.0	41.0
24	36.93053134468659	38.0	35.0	40.0	34.0	41.0
25	36.78827389102279	38.0	35.0	40.0	33.0	41.0
26	36.88471852939301	38.0	35.0	40.0	34.0	41.0
27	36.89505439116501	38.0	35.0	40.0	34.0	41.0
28	36.824927295745645	38.0	35.0	40.0	33.0	41.0
29	36.68188028713434	38.0	35.0	41.0	33.0	41.0
30	36.3626314103873	38.0	35.0	41.0	33.0	41.0
31	35.82218964651295	38.0	35.0	41.0	31.0	41.0
32	35.025169750731344	38.0	35.0	41.0	22.0	41.0
33	34.00753086393217	38.0	34.0	41.0	15.0	41.0
34	33.164019651463796	38.0	33.0	41.0	10.0	41.0
35	32.6318589003692	38.0	32.0	41.0	8.0	41.0
36	32.3657874807537	38.0	31.0	41.0	8.0	41.0
37	32.11706262632342	38.0	30.0	41.0	7.0	41.0
38	31.9781790512874	38.0	30.0	41.0	7.0	41.0
39	31.88420338419586	38.0	29.0	41.0	7.0	41.0
40	31.75068190464727	38.0	29.0	41.0	7.0	41.0
41	31.602218348483188	37.0	27.0	41.0	7.0	41.0
42	31.479336515913257	37.0	26.0	41.0	7.0	41.0
43	31.367447552272193	37.0	25.0	41.0	7.0	41.0
44	31.262677479700486	37.0	24.0	40.0	7.0	41.0
45	31.231535197170214	37.0	24.0	40.0	7.0	41.0
46	31.192418552965023	37.0	24.0	40.0	7.0	41.0
47	31.182291185285333	36.0	24.0	40.0	7.0	41.0
48	31.11925073958085	36.0	24.0	40.0	7.0	41.0
49	30.999873184005708	36.0	24.0	40.0	7.0	41.0
50	30.92233201000485	36.0	24.0	40.0	7.0	41.0
51	30.420005258923155	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	12.0
11	19.0
12	13.0
13	22.0
14	40.0
15	60.0
16	122.0
17	253.0
18	447.0
19	929.0
20	1616.0
21	2493.0
22	4116.0
23	7139.0
24	14237.0
25	31587.0
26	58393.0
27	75068.0
28	70434.0
29	55119.0
30	41492.0
31	34573.0
32	31774.0
33	35615.0
34	59769.0
35	80348.0
36	86692.0
37	113399.0
38	224445.0
39	365236.0
40	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.192413537643212	15.339290102692296	17.902907668641987	38.5653886910225
2	51.630731885911466	17.42100689033569	18.598891040700767	12.349370183052082
3	22.9909516429836	17.723789032637566	47.59139170164854	11.693867622730298
4	20.864025311612693	18.647897899511577	47.370001067547065	13.118075721328658
5	18.818562135896595	21.760478261087624	45.87808612453904	13.542873478476746
6	19.813888572445965	24.90716281095891	44.54164615758284	10.737302459012282
7	59.62357860406399	4.06147924767307	33.24792956768643	3.0670125805765185
8	60.99799172185312	3.565392273395222	32.67238556647701	2.7642304382746437
9	56.31432598015509	5.684437384781937	34.156920821681666	3.8443158133813085
10	28.023683782527048	24.532804861709668	38.57541933463875	8.868092021124536
11	20.33297437958678	18.224031559270713	48.337026759607745	13.10596730153476
12	19.511321372507297	16.777254512535798	50.02418101586059	13.68724309909631
13	17.25249207758273	17.58507956091574	50.82326507480352	14.339163286698007
14	14.548660443368778	20.00690681460433	49.32024477636322	16.124187965663676
15	13.911284689010643	19.828074768417515	52.14057517143444	14.120065371137397
16	15.80370890212456	18.87029159797467	51.28166548806604	14.044334011834728
17	16.442589253025133	18.16907796174456	48.65686099605724	16.731471789173067
18	16.11021671205533	19.339797366669462	48.92059527571015	15.629390645565058
19	17.242174844148874	19.902158236268942	48.17746787865501	14.678199040927176
20	19.060014057230553	19.28663495550335	47.80633406485384	13.847016922412255
21	18.3855249214923	18.789759859227082	48.40150946856934	14.42320575071128
22	17.29669855694862	18.73745721751379	47.499539665105466	16.46630456043212
23	16.564174983144937	19.183247678801596	48.47609446860158	15.776482869451891
24	16.151270703427542	18.179108605360806	49.61063190905359	16.058988782158064
25	16.249929248138777	19.16669711683479	48.1891464137225	16.394227221303943
26	15.453281202645512	20.86681956233436	48.085400899748734	15.594498335271398
27	15.884527230689757	21.08147533572206	47.91996692753504	15.114030506053137
28	14.97847352232499	20.32115254961049	48.99861935355368	15.701754574510845
29	16.148977984886688	19.245151079404724	48.58392388747624	16.02194704823235
30	16.454267788092622	19.999742069164153	47.19647093298598	16.349519209757236
31	16.768585170553184	21.32364373160004	45.16992268523195	16.737848412614824
32	17.977707611037435	22.17889939479395	44.12351161369412	15.719881380474494
33	18.1384128512606	23.938704169810197	41.19764451828909	16.725238460640114
34	17.757105098934385	24.73750163893552	39.89194130927125	17.613451952858842
35	18.300192803299794	25.156209362459457	37.87105321041496	18.672544623825786
36	18.402147130913512	27.59487376793246	36.462750846693794	17.54022825446023
37	19.433870474298985	27.93591565088488	36.1108185506723	16.519395324143833
38	19.33943912939745	26.978992249894855	35.00193089889613	18.679637721811563
39	19.215847270554402	27.14148867647807	35.35551108636886	18.287152966598676
40	19.52206849066756	26.926044781091953	35.99374661017981	17.558140118060674
41	17.961085401616224	28.05843279791191	35.04441783935638	18.936063961115494
42	18.846647938022084	28.15752122734955	34.404821013911786	18.591009820716575
43	19.259767160102683	27.270812331673262	34.753529174485195	18.715891333738856
44	18.583630132913193	27.182900905122292	34.67220931373919	19.56125964822533
45	18.441624878288888	26.417634444657	34.921255865239736	20.21948481181438
46	18.169292904107763	26.864284675397627	35.579910913555196	19.386511506939414
47	18.002139392988436	26.490499905783597	36.98183665383461	18.525524047393358
48	18.05128954670805	24.958247445947368	37.31657356079967	19.67388944654491
49	18.125659604377088	24.410932541772258	37.79130959366579	19.672098260184864
50	17.174038115012795	24.428629463009493	37.86582294624363	20.531509475734083
51	16.996495723005207	24.382345207465953	36.7304257363388	21.890733333190038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	467.0
1	821.5
2	1176.0
3	57439.0
4	113702.0
5	80151.5
6	46601.0
7	44445.0
8	42289.0
9	40384.5
10	38480.0
11	36675.0
12	34870.0
13	32701.5
14	30533.0
15	27845.5
16	25158.0
17	23050.0
18	20942.0
19	19582.5
20	18223.0
21	17082.5
22	15942.0
23	14754.0
24	13566.0
25	14561.5
26	15843.5
27	16130.0
28	18130.5
29	20131.0
30	21573.5
31	23016.0
32	24628.5
33	26241.0
34	29278.0
35	32315.0
36	35622.0
37	38929.0
38	41396.0
39	43863.0
40	48349.5
41	52836.0
42	58118.5
43	63401.0
44	64550.0
45	65699.0
46	72335.0
47	78971.0
48	83521.5
49	88072.0
50	85832.0
51	83592.0
52	74411.0
53	65230.0
54	58779.0
55	52328.0
56	48630.0
57	44932.0
58	44166.0
59	43400.0
60	42849.5
61	42299.0
62	38515.5
63	34732.0
64	29025.5
65	23319.0
66	18987.5
67	14656.0
68	11907.5
69	9159.0
70	7620.0
71	6081.0
72	5090.0
73	4099.0
74	3267.0
75	1808.5
76	1182.0
77	886.5
78	591.0
79	492.0
80	393.0
81	249.0
82	105.0
83	74.0
84	43.0
85	31.5
86	20.0
87	13.0
88	6.0
89	6.5
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1395723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.3336004441722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4658853665298	26.48884071750295
2	8.519220287278376	5.679525703040465
3	2.883381766512696	2.8834048719883687
4	1.4221773266866249	1.8962516307413164
5	0.8370599282819515	1.3951110598589005
6	0.5958175501199545	1.1916446491994466
7	0.43299612908111185	1.0103323972464096
8	0.33838209864762564	0.9023594939024332
9	0.2832839225389937	0.8498585777555379
>10	4.706162622649375	39.223302022968376
>50	0.44301464516407874	9.007753220634465
>100	0.06801114965701167	4.080189741566189
>500	0.00285208043238887	0.6937192764315601
>1k	0.0015357356174401607	0.7996127523194894
>5k	0.0	0.0
>10k+	2.1939080249145154E-4	3.8980938848441324
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53303	3.8190242619774843	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	2478	0.17754239200758318	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	2128	0.15246578296696406	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1541	0.11040872723312577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09923172434644983	0.0
2	0.0	0.0	0.0	0.5549811817961013	0.0
3	0.0	0.0	0.0	0.8093296449223807	0.0
4	0.0	0.0	0.0	1.0536474644324125	0.0
5	0.0	0.0	0.0	1.698474554048332	0.0
6	0.0	0.0	0.0	2.051123324613838	0.0
7	0.0	0.0	0.0	2.434580500572105	0.0
8	0.0	0.0	0.0	3.0276781281099474	0.0
9	0.0	0.0	0.0	3.2582396363748396	0.0
10	0.0	0.0	0.0	3.7528220141102495	0.0
11	0.0	0.0	0.0	4.482837927009872	0.0
12	0.0	0.0	0.0	5.08102252381024	0.0
13	0.0	0.0	0.0	5.307070242447821	0.0
14	0.0	0.0	0.0	5.403937600799012	0.0
15	0.0	0.0	0.0	5.574673484638428	0.0
16	0.0	0.0	0.0	5.946738715346813	0.0
17	0.0	0.0	0.0	6.42054333130571	0.0
18	0.0	0.0	0.0	6.94142032480657	0.0
19	0.0	0.0	0.0	7.25330169381747	0.0
20	0.0	0.0	0.0	7.564179998466744	0.0
21	0.0	0.0	0.0	7.940973961165647	0.0
22	0.0	0.0	0.0	8.36641654540335	0.0
23	0.0	0.0	0.0	8.775451862583049	0.0
24	0.0	0.0	0.0	9.09428303467092	0.0
25	0.0	0.0	0.0	9.371415388296962	0.0
26	0.0	0.0	0.0	9.637370739036328	0.0
27	7.164745440176883E-5	0.0	0.0	9.899743717055605	0.0
28	7.164745440176883E-5	0.0	0.0	10.169639677787067	0.0
29	1.4329490880353766E-4	0.0	0.0	10.45572796321333	0.0
30	1.4329490880353766E-4	0.0	0.0	10.781652233286977	0.0
31	1.4329490880353766E-4	0.0	0.0	11.106716733907803	0.0
32	1.4329490880353766E-4	0.0	0.0	11.3942379684221	0.0
33	1.4329490880353766E-4	0.0	0.0	11.671441969502544	0.0
34	1.4329490880353766E-4	0.0	0.0	11.94749961131256	0.0
35	1.4329490880353766E-4	0.0	0.0	12.26059898704829	0.0
36	1.4329490880353766E-4	0.0	0.0	12.572193766241583	0.0
37	1.4329490880353766E-4	0.0	0.0	12.882212301438036	0.0
38	1.4329490880353766E-4	0.0	0.0	13.177543108482126	0.0
39	1.4329490880353766E-4	0.0	0.0	13.462341739729158	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	25	3.891236E-5	45.000004	1
CACTCCG	25	3.891236E-5	45.000004	11
GTATACG	25	3.891236E-5	45.000004	1
ATTAGCG	40	6.8175723E-9	45.0	1
CAACGCG	20	7.033807E-4	45.0	1
ACGGCTA	20	7.033807E-4	45.0	34
TCGTAAG	20	7.033807E-4	45.0	1
CGTAATG	40	6.8175723E-9	45.0	1
CGTTTTT	39970	0.0	44.560917	1
ACGGGTC	90	0.0	40.000004	5
GTTTTTT	44840	0.0	39.90187	2
TAACGCG	40	3.4589357E-7	39.375	1
GTTACGG	70	0.0	38.57143	2
GTTAACG	35	6.249251E-6	38.57143	1
TCAACGG	35	6.249251E-6	38.57143	2
CGAATAT	135	0.0	38.333332	14
CTACGAA	130	0.0	38.07692	11
TACGGGT	90	0.0	37.500004	4
GTAAGCG	90	0.0	37.500004	1
TAAGCGG	180	0.0	37.500004	2
>>END_MODULE