SRR2934916.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934916.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378000
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13325	3.525132275132275	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1836	0.48571428571428565	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1808	0.4783068783068783	RNA PCR Primer, Index 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	918	0.24285714285714283	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	748	0.19788359788359788	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	688	0.182010582010582	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	658	0.17407407407407408	RNA PCR Primer, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	538	0.14232804232804233	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	520	0.1375661375661376	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	506	0.13386243386243388	No Hit
ACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	454	0.1201058201058201	RNA PCR Primer, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.0272724E-4	45.000004	1
CCGGGAT	20	7.0272724E-4	45.000004	6
CTATCGG	20	7.0272724E-4	45.000004	2
CCGTGTG	20	7.0272724E-4	45.000004	20
CGATTTA	20	7.0272724E-4	45.000004	12
CTCCACG	20	7.0272724E-4	45.000004	1
AATGGAT	20	7.0272724E-4	45.000004	15
TGCGGGT	40	6.7939254E-9	45.000004	4
CGTTCAG	20	7.0272724E-4	45.000004	38
TTGAGCG	20	7.0272724E-4	45.000004	1
TAGCAAG	20	7.0272724E-4	45.000004	1
ACCTAGG	20	7.0272724E-4	45.000004	2
TCCACGG	20	7.0272724E-4	45.000004	2
AGTCTCG	20	7.0272724E-4	45.000004	30
CTCGCGC	20	7.0272724E-4	45.000004	33
GCGATAC	20	7.0272724E-4	45.000004	9
CCCCAGT	20	7.0272724E-4	45.000004	39
CGAGCCG	20	7.0272724E-4	45.000004	24
CGTGTGC	20	7.0272724E-4	45.000004	21
TGCTATG	20	7.0272724E-4	45.000004	1