##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934916.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378000
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.468195767195766	34.0	31.0	34.0	30.0	34.0
2	32.63538888888889	34.0	31.0	34.0	31.0	34.0
3	32.70089417989418	34.0	31.0	34.0	31.0	34.0
4	36.12712433862434	37.0	37.0	37.0	35.0	37.0
5	36.0035	37.0	35.0	37.0	35.0	37.0
6	35.98442857142857	37.0	35.0	37.0	35.0	37.0
7	36.16006349206349	37.0	36.0	37.0	35.0	37.0
8	36.176486772486776	37.0	37.0	37.0	35.0	37.0
9	37.98443650793651	39.0	39.0	39.0	35.0	39.0
10	37.56715873015873	39.0	37.0	39.0	35.0	39.0
11	37.40879894179894	39.0	37.0	39.0	34.0	39.0
12	37.20984126984127	39.0	37.0	39.0	34.0	39.0
13	37.04730423280423	39.0	37.0	39.0	33.0	39.0
14	38.21366666666667	40.0	37.0	41.0	33.0	41.0
15	38.29123544973545	40.0	37.0	41.0	33.0	41.0
16	38.44856878306879	40.0	37.0	41.0	34.0	41.0
17	38.48407407407407	40.0	37.0	41.0	34.0	41.0
18	38.048084656084654	39.0	37.0	40.0	34.0	41.0
19	37.55835714285714	37.0	37.0	40.0	34.0	41.0
20	36.99011375661376	37.0	35.0	40.0	34.0	41.0
21	36.92420634920635	37.0	35.0	40.0	34.0	41.0
22	36.72677248677249	37.0	35.0	40.0	33.0	41.0
23	36.61012962962963	36.0	35.0	40.0	33.0	41.0
24	36.421632275132275	36.0	35.0	40.0	33.0	41.0
25	36.26244708994709	36.0	35.0	40.0	33.0	41.0
26	36.415433862433865	36.0	35.0	40.0	33.0	41.0
27	36.45158994708995	36.0	35.0	40.0	33.0	41.0
28	36.39180423280423	36.0	35.0	40.0	33.0	41.0
29	36.30883333333333	36.0	35.0	40.0	33.0	41.0
30	36.017798941798944	36.0	35.0	40.0	32.0	41.0
31	35.4189708994709	36.0	35.0	40.0	30.0	41.0
32	34.70436772486772	36.0	35.0	40.0	23.0	41.0
33	33.736478835978836	36.0	34.0	40.0	16.0	41.0
34	32.95071957671958	36.0	33.0	40.0	10.0	41.0
35	32.54042857142857	36.0	33.0	40.0	10.0	41.0
36	32.33603703703704	36.0	32.0	40.0	9.0	41.0
37	32.15971428571429	36.0	31.0	40.0	8.0	41.0
38	32.04564285714286	36.0	31.0	40.0	8.0	41.0
39	32.029272486772484	36.0	31.0	40.0	8.0	41.0
40	31.883899470899472	36.0	31.0	40.0	8.0	41.0
41	31.718481481481483	36.0	30.0	40.0	7.0	41.0
42	31.53962962962963	36.0	29.0	40.0	7.0	41.0
43	31.391153439153438	35.0	29.0	40.0	7.0	41.0
44	31.24124074074074	35.0	28.0	40.0	7.0	41.0
45	31.189537037037038	35.0	27.0	40.0	7.0	41.0
46	31.13901851851852	35.0	27.0	40.0	7.0	41.0
47	31.095613756613755	35.0	27.0	40.0	7.0	41.0
48	31.067089947089947	35.0	27.0	40.0	7.0	41.0
49	30.97116402116402	35.0	27.0	40.0	7.0	41.0
50	30.843777777777778	35.0	27.0	40.0	7.0	41.0
51	30.253740740740742	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	3.0
12	5.0
13	8.0
14	9.0
15	11.0
16	34.0
17	73.0
18	137.0
19	283.0
20	490.0
21	787.0
22	1334.0
23	2095.0
24	4002.0
25	8639.0
26	15446.0
27	19277.0
28	17312.0
29	13195.0
30	10526.0
31	9547.0
32	9997.0
33	12595.0
34	23915.0
35	36055.0
36	27053.0
37	33977.0
38	61007.0
39	70141.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.771957671957672	15.638359788359788	19.233333333333334	36.35634920634921
2	48.38439153439153	18.892592592592592	19.256084656084656	13.466931216931217
3	22.73227513227513	18.691798941798943	46.014550264550266	12.561375661375662
4	21.294708994708994	19.582275132275132	45.3510582010582	13.771957671957672
5	18.73095238095238	21.134126984126983	45.164550264550265	14.97037037037037
6	18.96640211640212	24.69047619047619	43.952380952380956	12.39074074074074
7	59.09973544973545	4.4944444444444445	32.46031746031746	3.945502645502646
8	59.9441798941799	3.766137566137566	31.432804232804234	4.856878306878307
9	55.785185185185185	4.9378306878306875	33.08227513227513	6.194708994708995
10	33.60846560846561	16.94814814814815	38.485978835978834	10.957407407407407
11	26.824074074074073	17.72142857142857	41.6457671957672	13.808730158730159
12	23.20132275132275	16.973280423280425	44.91587301587302	14.909523809523808
13	17.33068783068783	22.193386243386243	45.980687830687835	14.495238095238093
14	13.661904761904761	25.066402116402113	45.55846560846561	15.713227513227512
15	11.798941798941799	19.964285714285715	54.36507936507936	13.871693121693122
16	13.265608465608466	17.807142857142857	51.830423280423275	17.096825396825395
17	13.17962962962963	17.57063492063492	46.2489417989418	23.00079365079365
18	15.52089947089947	18.617460317460317	48.41587301587302	17.445767195767196
19	17.214550264550265	19.734391534391534	45.34391534391534	17.70714285714286
20	19.598412698412698	19.201322751322753	45.69656084656085	15.503703703703703
21	16.775132275132275	20.946825396825396	45.621428571428574	16.656613756613755
22	15.574867724867724	20.005555555555553	44.20793650793651	20.211640211640212
23	14.50873015873016	20.96058201058201	45.00343915343915	19.527248677248675
24	16.62195767195767	18.08941798941799	47.26190476190476	18.02671957671958
25	14.056878306878307	19.92222222222222	46.50608465608465	19.514814814814816
26	14.88862433862434	21.963227513227512	45.56878306878307	17.57936507936508
27	15.09047619047619	22.176455026455027	45.83783068783069	16.895238095238096
28	13.056084656084657	20.94126984126984	48.733333333333334	17.26931216931217
29	16.279894179894182	18.83862433862434	47.51772486772487	17.363756613756614
30	17.364285714285714	20.29867724867725	44.27010582010582	18.066931216931216
31	18.322751322751323	23.727513227513228	40.17089947089947	17.77883597883598
32	20.38068783068783	22.770899470899472	39.91111111111111	16.937301587301587
33	19.154497354497355	24.73174603174603	37.348412698412695	18.765343915343916
34	17.598148148148148	25.601851851851855	37.342063492063495	19.45793650793651
35	19.124867724867727	23.817195767195766	35.46402116402116	21.59391534391534
36	21.03386243386243	26.637037037037036	33.800529100529104	18.52857142857143
37	18.738624338624337	27.35978835978836	36.267724867724866	17.633862433862436
38	18.789682539682538	27.900264550264552	33.96349206349206	19.346560846560845
39	20.437037037037037	25.858994708994707	35.18465608465608	18.51931216931217
40	20.845502645502645	24.42037037037037	35.681481481481484	19.0526455026455
41	17.08068783068783	25.75899470899471	34.16534391534392	22.994973544973547
42	18.893915343915342	26.126190476190477	33.77566137566138	21.204232804232802
43	20.513227513227513	26.475925925925925	32.77513227513227	20.235714285714284
44	19.007936507936506	27.235978835978834	32.33862433862434	21.417460317460318
45	19.1031746031746	26.09391534391534	31.603174603174605	23.19973544973545
46	21.0010582010582	24.942063492063493	33.73148148148148	20.325396825396822
47	17.41269841269841	25.673809523809528	36.16058201058201	20.752910052910053
48	17.78888888888889	23.261904761904763	36.37460317460317	22.574603174603176
49	18.151587301587302	22.25925925925926	38.06560846560846	21.523544973544972
50	17.66878306878307	23.273015873015872	36.672751322751324	22.385449735449736
51	16.960317460317462	23.683597883597884	33.50925925925926	25.846825396825395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	289.0
2	341.0
3	12523.0
4	24705.0
5	17057.0
6	9409.0
7	9482.5
8	9556.0
9	9799.0
10	10042.0
11	9834.5
12	9627.0
13	8933.5
14	8240.0
15	7529.0
16	6818.0
17	6151.5
18	5485.0
19	4979.0
20	4473.0
21	3995.0
22	3517.0
23	3293.5
24	3070.0
25	2969.0
26	3160.5
27	3453.0
28	3319.0
29	3185.0
30	3626.0
31	4067.0
32	4640.0
33	5213.0
34	4984.5
35	4756.0
36	5858.0
37	6960.0
38	7649.0
39	8338.0
40	10791.0
41	13244.0
42	15039.5
43	16835.0
44	20339.5
45	23844.0
46	27102.5
47	30361.0
48	32359.0
49	34357.0
50	34350.0
51	34343.0
52	28698.0
53	23053.0
54	19529.5
55	16006.0
56	14036.5
57	12067.0
58	11233.5
59	10400.0
60	9734.5
61	9069.0
62	8161.5
63	7254.0
64	6075.5
65	4897.0
66	3995.0
67	3093.0
68	2593.5
69	2094.0
70	1606.0
71	1118.0
72	916.0
73	714.0
74	575.0
75	332.0
76	228.0
77	175.0
78	122.0
79	79.5
80	37.0
81	31.0
82	25.0
83	31.5
84	38.0
85	20.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	378000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.34288046492489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32886082599877	30.816830084310077
2	9.67680388028957	7.614266766895078
3	3.699579740100703	4.366563704557196
4	1.7900093317487704	2.8169649268036783
5	1.0437909571226593	2.053287142822316
6	0.6591790369612909	1.5560401233691423
7	0.4337688034943559	1.1945999929704356
8	0.3147148358037345	0.9905430532451831
9	0.24341896121380763	0.8619122784537919
>10	3.5082041952612872	33.52594484547884
>50	0.26709608137074137	6.470357016268507
>100	0.027794262274620292	1.9864476115897707
>500	0.004745361851764439	1.2204597570669697
>1k	0.0013558176719326972	0.9718870967552531
>5k	0.0	0.0
>10k+	6.779088359663486E-4	3.5538955994137615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13325	3.525132275132275	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1836	0.48571428571428565	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1808	0.4783068783068783	RNA PCR Primer, Index 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	918	0.24285714285714283	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	748	0.19788359788359788	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	688	0.182010582010582	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	658	0.17407407407407408	RNA PCR Primer, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	538	0.14232804232804233	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	520	0.1375661375661376	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	506	0.13386243386243388	No Hit
ACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	454	0.1201058201058201	RNA PCR Primer, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.291005291005291E-4	0.0	0.0	0.25846560846560845	0.0
2	5.291005291005291E-4	0.0	0.0	1.6222222222222222	0.0
3	5.291005291005291E-4	0.0	0.0	2.2293650793650794	0.0
4	5.291005291005291E-4	0.0	0.0	2.805026455026455	0.0
5	5.291005291005291E-4	0.0	0.0	4.4034391534391535	0.0
6	5.291005291005291E-4	0.0	0.0	5.187037037037037	0.0
7	5.291005291005291E-4	0.0	0.0	6.064550264550265	0.0
8	5.291005291005291E-4	0.0	0.0	7.474603174603175	0.0
9	5.291005291005291E-4	0.0	0.0	8.003174603174603	0.0
10	5.291005291005291E-4	0.0	0.0	9.203174603174602	0.0
11	5.291005291005291E-4	0.0	0.0	10.797883597883597	0.0
12	5.291005291005291E-4	0.0	0.0	12.256613756613756	0.0
13	5.291005291005291E-4	0.0	0.0	12.742857142857142	0.0
14	5.291005291005291E-4	0.0	0.0	12.92116402116402	0.0
15	5.291005291005291E-4	0.0	0.0	13.262698412698413	0.0
16	5.291005291005291E-4	0.0	0.0	13.993650793650794	0.0
17	5.291005291005291E-4	0.0	0.0	14.950264550264551	0.0
18	5.291005291005291E-4	0.0	0.0	16.12037037037037	0.0
19	5.291005291005291E-4	0.0	0.0	16.747089947089947	0.0
20	5.291005291005291E-4	0.0	0.0	17.31984126984127	0.0
21	5.291005291005291E-4	0.0	0.0	18.030423280423282	0.0
22	5.291005291005291E-4	0.0	0.0	18.738359788359787	0.0
23	5.291005291005291E-4	0.0	0.0	19.47010582010582	0.0
24	5.291005291005291E-4	0.0	0.0	20.021693121693122	0.0
25	5.291005291005291E-4	0.0	0.0	20.534656084656085	0.0
26	5.291005291005291E-4	0.0	0.0	20.992857142857144	0.0
27	5.291005291005291E-4	0.0	0.0	21.43994708994709	0.0
28	5.291005291005291E-4	0.0	0.0	21.862433862433864	0.0
29	5.291005291005291E-4	0.0	0.0	22.313756613756613	0.0
30	5.291005291005291E-4	0.0	0.0	22.83941798941799	0.0
31	5.291005291005291E-4	0.0	0.0	23.344444444444445	0.0
32	5.291005291005291E-4	0.0	0.0	23.823809523809523	0.0
33	5.291005291005291E-4	0.0	0.0	24.265608465608466	0.0
34	5.291005291005291E-4	0.0	0.0	24.725132275132275	0.0
35	5.291005291005291E-4	0.0	0.0	25.26825396825397	0.0
36	5.291005291005291E-4	0.0	0.0	25.761111111111113	0.0
37	5.291005291005291E-4	0.0	0.0	26.197089947089946	0.0
38	5.291005291005291E-4	0.0	0.0	26.63968253968254	0.0
39	5.291005291005291E-4	0.0	0.0	27.053174603174604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.0272724E-4	45.000004	1
CCGGGAT	20	7.0272724E-4	45.000004	6
CTATCGG	20	7.0272724E-4	45.000004	2
CCGTGTG	20	7.0272724E-4	45.000004	20
CGATTTA	20	7.0272724E-4	45.000004	12
CTCCACG	20	7.0272724E-4	45.000004	1
AATGGAT	20	7.0272724E-4	45.000004	15
TGCGGGT	40	6.7939254E-9	45.000004	4
CGTTCAG	20	7.0272724E-4	45.000004	38
TTGAGCG	20	7.0272724E-4	45.000004	1
TAGCAAG	20	7.0272724E-4	45.000004	1
ACCTAGG	20	7.0272724E-4	45.000004	2
TCCACGG	20	7.0272724E-4	45.000004	2
AGTCTCG	20	7.0272724E-4	45.000004	30
CTCGCGC	20	7.0272724E-4	45.000004	33
GCGATAC	20	7.0272724E-4	45.000004	9
CCCCAGT	20	7.0272724E-4	45.000004	39
CGAGCCG	20	7.0272724E-4	45.000004	24
CGTGTGC	20	7.0272724E-4	45.000004	21
TGCTATG	20	7.0272724E-4	45.000004	1
>>END_MODULE