##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934915.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752334
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.612606900658484	34.0	31.0	34.0	31.0	34.0
2	32.77271663915229	34.0	31.0	34.0	31.0	34.0
3	32.81968646904168	34.0	31.0	34.0	31.0	34.0
4	36.208701188567844	37.0	37.0	37.0	35.0	37.0
5	36.09923916771009	37.0	35.0	37.0	35.0	37.0
6	36.08896314668751	37.0	36.0	37.0	35.0	37.0
7	36.23738126948935	37.0	37.0	37.0	35.0	37.0
8	36.237624512517044	37.0	37.0	37.0	35.0	37.0
9	38.02481078882518	39.0	39.0	39.0	35.0	39.0
10	37.64278232806174	39.0	38.0	39.0	35.0	39.0
11	37.52029550704873	39.0	37.0	39.0	35.0	39.0
12	37.424533518357535	39.0	37.0	39.0	35.0	39.0
13	37.35454997381482	39.0	37.0	39.0	34.0	39.0
14	38.62433041707539	40.0	38.0	41.0	34.0	41.0
15	38.70918501622949	40.0	38.0	41.0	35.0	41.0
16	38.800931501168364	40.0	38.0	41.0	35.0	41.0
17	38.83317781729923	40.0	38.0	41.0	35.0	41.0
18	38.32982957037699	39.0	38.0	41.0	35.0	41.0
19	37.823160989666825	38.0	37.0	41.0	35.0	41.0
20	37.25190407452009	38.0	35.0	41.0	34.0	41.0
21	37.16572027849333	38.0	35.0	40.0	34.0	41.0
22	36.97079621551067	38.0	35.0	40.0	34.0	41.0
23	36.886131691509355	37.0	35.0	40.0	34.0	41.0
24	36.67646284761821	37.0	35.0	40.0	33.0	41.0
25	36.51800530083713	37.0	35.0	40.0	33.0	41.0
26	36.60575621997677	37.0	35.0	40.0	33.0	41.0
27	36.65444071383189	37.0	35.0	40.0	33.0	41.0
28	36.59933752827866	37.0	35.0	40.0	33.0	41.0
29	36.458470572910436	37.0	35.0	40.0	33.0	41.0
30	36.134377816235876	37.0	35.0	40.0	32.0	41.0
31	35.602350817589	37.0	35.0	40.0	30.0	41.0
32	34.849364776814554	37.0	35.0	40.0	23.0	41.0
33	33.88417644290966	37.0	34.0	40.0	16.0	41.0
34	33.085023407156925	37.0	33.0	40.0	12.0	41.0
35	32.57632248442846	37.0	33.0	40.0	10.0	41.0
36	32.303857063485104	37.0	31.0	40.0	8.0	41.0
37	32.085719906318204	37.0	31.0	40.0	8.0	41.0
38	31.95612719882393	37.0	30.0	40.0	8.0	41.0
39	31.885621279910254	37.0	30.0	40.0	7.0	41.0
40	31.746797300135313	36.0	30.0	40.0	7.0	41.0
41	31.58707834552207	36.0	29.0	40.0	7.0	41.0
42	31.42597436776751	36.0	29.0	40.0	7.0	41.0
43	31.285116716777388	36.0	27.0	40.0	7.0	41.0
44	31.17694534608299	36.0	26.0	40.0	7.0	41.0
45	31.144530221949292	35.0	25.0	40.0	7.0	41.0
46	31.0989467443981	35.0	26.0	40.0	7.0	41.0
47	31.09067382306263	35.0	26.0	40.0	7.0	41.0
48	31.05007483378393	35.0	26.0	40.0	7.0	41.0
49	30.922466617220543	35.0	26.0	40.0	7.0	41.0
50	30.83062443010684	35.0	25.0	40.0	7.0	41.0
51	30.29080700858927	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	9.0
10	5.0
11	10.0
12	8.0
13	11.0
14	18.0
15	25.0
16	52.0
17	135.0
18	246.0
19	544.0
20	913.0
21	1357.0
22	2292.0
23	3989.0
24	7684.0
25	16882.0
26	30819.0
27	39174.0
28	36128.0
29	28195.0
30	21662.0
31	18848.0
32	18695.0
33	22719.0
34	41164.0
35	58539.0
36	52706.0
37	67739.0
38	123044.0
39	158611.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.404493217108357	15.809600523171888	17.72749869074108	37.05840756897867
2	50.43757161048151	18.86489245468103	17.792230578439895	12.905305356397555
3	23.013980492706697	18.19417971273397	46.0621213450409	12.729718449518431
4	21.921912342124642	19.067462058075268	45.192560750943066	13.818064848857023
5	19.097103148335712	22.0162853200839	44.12561442125439	14.760997110325999
6	20.024882565456302	24.755095476211363	44.46269874816239	10.757323210169952
7	58.2943214051206	5.12564898037308	32.54432206971904	4.0357075447872885
8	58.27518097015422	4.662689709623651	32.66939949543687	4.392729824785268
9	53.81678881985926	6.298798140187737	34.442681043260045	5.441731996692958
10	30.59811200876207	19.023465641590036	39.32535283530985	11.053069514338047
11	24.3379934975689	18.33613793873466	43.40160088471344	13.924267678983004
12	22.04034378347915	16.562723471224217	46.96158886877371	14.435343876522927
13	17.723511100123083	20.059973362894674	48.36043565756699	13.856079879415258
14	13.70614647217858	22.95044488219328	47.31462355815369	16.028785087474446
15	13.515406720951068	19.14389087825354	52.61665696352949	14.724045437265895
16	14.73933120130155	18.550271554921085	50.4984488272496	16.211948416527765
17	15.55492640236916	18.3236434881316	46.50062339333328	19.62080671616596
18	15.954615901979704	18.915534855529593	48.17168970164847	16.95815954084223
19	18.001313246510193	19.449739078653895	46.218036138204575	16.330911536631337
20	19.80117873178668	19.600868763075972	45.41972049648162	15.178232008655728
21	17.345753348911522	20.3158437608828	46.639657386214104	15.69874550399158
22	15.72187352957596	19.968258778680745	45.217283812774646	19.09258387896865
23	16.00844837532266	20.218014871054613	46.08139469969455	17.692142053928176
24	16.631974628290095	18.582305199552327	47.66792408690821	17.11779608524937
25	15.412037738557608	19.68673488105017	46.9306185816406	17.97060879875162
26	14.56892816222582	22.70015711107035	46.11289666557673	16.618018061127103
27	15.825285046269343	21.85691461505129	46.439480337190666	15.8783200014887
28	13.798392735141574	21.326565062857718	48.53748468100604	16.337557520994665
29	15.98186443786935	20.435737318797237	46.70080044235672	16.881597800976696
30	16.80982116985275	21.54375583185128	44.6514447040809	16.99497829421507
31	17.262811464057187	22.369187089776617	42.57470751022817	17.793293935938028
32	19.10720504456797	22.827095412409914	41.23341494602131	16.8322845970008
33	18.933080254248775	24.5025480704049	38.86611531580388	17.698256359542437
34	17.413409469730198	24.220226654650727	38.86172896612409	19.504634909494985
35	19.06360738714454	24.964709823030727	36.03014086828457	19.941541921540168
36	19.665068972025722	27.10750810145494	35.146490787336475	18.080932139182863
37	19.756251877490584	26.784779100771733	36.201607264858424	17.25736175687926
38	20.008134684860714	27.01021089037582	34.171657800923526	18.809996623839943
39	20.995329202189453	25.79532494875946	34.01707220463251	19.19227364441857
40	20.970606140357873	25.059481560051783	34.61879962888823	19.351112670702108
41	17.882217206719357	26.967809510137787	33.166120366752	21.98385291639086
42	18.780488453266766	27.53245234164613	33.45761324092757	20.229445964159535
43	19.918148056581252	26.582475336752026	33.985968997812144	19.51340760885458
44	19.01030659255065	26.325807420640302	33.64742255434422	21.016463432464835
45	19.30153362735168	25.47166551026539	33.36124646765931	21.86555439472362
46	20.222932899483475	25.9124271932413	33.95047944131197	19.914160465963256
47	18.23525189609934	25.285445028404936	36.4883150302924	19.990988045203327
48	18.586558629544854	23.891516268040526	35.93736292657251	21.584562175842112
49	18.357538008384573	23.638835942546795	37.30178351636374	20.70184253270489
50	17.567064628210343	23.520537420879556	37.13869106008767	21.773706890822428
51	17.616111992811703	23.85921678403475	34.51179928063865	24.012871942514895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	570.0
2	805.0
3	28559.0
4	56313.0
5	39418.0
6	22523.0
7	21525.5
8	20528.0
9	20004.0
10	19480.0
11	18897.0
12	18314.0
13	17097.5
14	15881.0
15	14448.5
16	13016.0
17	11868.5
18	10721.0
19	9807.0
20	8893.0
21	8116.0
22	7339.0
23	7048.5
24	6758.0
25	6925.0
26	7488.0
27	7884.0
28	8557.5
29	9231.0
30	9851.5
31	10472.0
32	10528.0
33	10584.0
34	11896.5
35	13209.0
36	14401.5
37	15594.0
38	16682.5
39	17771.0
40	21865.0
41	25959.0
42	29886.5
43	33814.0
44	37731.0
45	41648.0
46	46798.0
47	51948.0
48	55144.0
49	58340.0
50	57553.5
51	56767.0
52	48235.0
53	39703.0
54	34923.5
55	30144.0
56	27139.0
57	24134.0
58	22464.5
59	20795.0
60	20605.5
61	20416.0
62	18556.0
63	16696.0
64	14999.0
65	13302.0
66	11123.0
67	8944.0
68	7547.5
69	6151.0
70	5085.0
71	4019.0
72	3302.0
73	2585.0
74	2092.0
75	1386.0
76	1173.0
77	1044.0
78	915.0
79	632.0
80	349.0
81	229.0
82	109.0
83	62.5
84	16.0
85	34.5
86	53.0
87	30.5
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.18286997734425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90015158032107	27.407509042658436
2	9.801907235885757	6.897184556203166
3	3.6809541298055297	3.8851959162454883
4	1.8201939332273684	2.5615858594515726
5	1.0211039116964482	1.796268307928687
6	0.6569730699256785	1.3868518858687102
7	0.47443854165562804	1.1684476666317545
8	0.3613121731321638	1.0169599366832487
9	0.2692454241212677	0.8525544073968119
>10	3.3134847091956563	29.733426807095746
>50	0.629827017433243	13.927082660844132
>100	0.06502184829745052	3.7295548530426075
>500	0.0034627019803376013	0.8127267471569329
>1k	0.0015389786579278228	0.9535055308083669
>5k	0.0	0.0
>10k+	3.847446644819557E-4	3.871145821984338
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28598	3.801237216449077	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2511	0.3337613347263317	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2046	0.27195368014738136	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	1327	0.17638442500272486	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1160	0.15418683722920937	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	933	0.12401406821970029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.987590617996794E-4	0.0	0.0	0.19020807247844707	0.0
2	3.987590617996794E-4	0.0	0.0	1.0828966921606626	0.0
3	3.987590617996794E-4	0.0	0.0	1.5776237681667982	0.0
4	3.987590617996794E-4	0.0	0.0	2.037791725483628	0.0
5	3.987590617996794E-4	0.0	0.0	3.273811897375368	0.0
6	3.987590617996794E-4	0.0	0.0	3.9204661759271815	0.0
7	3.987590617996794E-4	0.0	0.0	4.599818697546568	0.0
8	3.987590617996794E-4	0.0	0.0	5.6086791238997575	0.0
9	3.987590617996794E-4	0.0	0.0	6.007571105386703	0.0
10	3.987590617996794E-4	0.0	0.0	6.865301847317814	0.0
11	3.987590617996794E-4	0.0	0.0	8.13814077258239	0.0
12	3.987590617996794E-4	0.0	0.0	9.154710540797039	0.0
13	3.987590617996794E-4	0.0	0.0	9.536721722001133	0.0
14	3.987590617996794E-4	0.0	0.0	9.699947097964468	0.0
15	3.987590617996794E-4	0.0	0.0	10.014036318975348	0.0
16	3.987590617996794E-4	0.0	0.0	10.673185048130218	0.0
17	3.987590617996794E-4	0.0	0.0	11.457942881751988	0.0
18	3.987590617996794E-4	0.0	0.0	12.441681487211797	0.0
19	3.987590617996794E-4	0.0	0.0	12.94464958382846	0.0
20	3.987590617996794E-4	0.0	0.0	13.423293377675341	0.0
21	3.987590617996794E-4	0.0	0.0	14.04495875502104	0.0
22	3.987590617996794E-4	0.0	0.0	14.628742021495771	0.0
23	3.987590617996794E-4	0.0	0.0	15.222228425140962	0.0
24	3.987590617996794E-4	0.0	0.0	15.720544332703295	0.0
25	3.987590617996794E-4	0.0	0.0	16.137779231033026	0.0
26	3.987590617996794E-4	0.0	0.0	16.524708440665982	0.0
27	3.987590617996794E-4	0.0	0.0	16.89648480595055	0.0
28	3.987590617996794E-4	0.0	0.0	17.286072409328835	0.0
29	3.987590617996794E-4	0.0	0.0	17.704636504531233	0.0
30	3.987590617996794E-4	0.0	0.0	18.166532417782527	0.0
31	3.987590617996794E-4	0.0	0.0	18.60423694795131	0.0
32	5.316787490662392E-4	0.0	0.0	19.033966296884095	0.0
33	5.316787490662392E-4	0.0	0.0	19.42288930182605	0.0
34	5.316787490662392E-4	0.0	0.0	19.79692530179415	0.0
35	5.316787490662392E-4	0.0	0.0	20.221204943549008	0.0
36	5.316787490662392E-4	0.0	0.0	20.618767728163288	0.0
37	5.316787490662392E-4	0.0	0.0	21.018058468712034	0.0
38	5.316787490662392E-4	0.0	0.0	21.3914298702438	0.0
39	5.316787490662392E-4	0.0	0.0	21.763472074902904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTA	30	2.1644973E-6	45.000004	16
GGTCGAG	30	2.1644973E-6	45.000004	7
TATACGG	30	2.1644973E-6	45.000004	2
TGTGTCG	30	2.1644973E-6	45.000004	6
TCACGAC	20	7.031732E-4	45.0	25
TCGTTAG	25	3.8895167E-5	45.0	1
CGACGGT	20	7.031732E-4	45.0	28
CGGCCGT	20	7.031732E-4	45.0	15
GTTAGGC	20	7.031732E-4	45.0	14
TGAGTGC	25	3.8895167E-5	45.0	13
TTATCCG	20	7.031732E-4	45.0	31
ACGGCGT	20	7.031732E-4	45.0	31
CCATACG	20	7.031732E-4	45.0	27
AACGACT	20	7.031732E-4	45.0	32
CGGTCTA	20	7.031732E-4	45.0	31
GACGATG	20	7.031732E-4	45.0	1
CGCAATC	25	3.8895167E-5	45.0	20
ACTCACG	25	3.8895167E-5	45.0	1
TCGACAG	20	7.031732E-4	45.0	1
TGTCGAA	20	7.031732E-4	45.0	36
>>END_MODULE