##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934913.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287791
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57848051430706	34.0	31.0	34.0	31.0	34.0
2	32.74373248454136	34.0	31.0	34.0	31.0	34.0
3	32.78618890798274	34.0	31.0	34.0	31.0	34.0
4	36.18986233014519	37.0	37.0	37.0	35.0	37.0
5	36.083134608022576	37.0	35.0	37.0	35.0	37.0
6	36.06970230417824	37.0	35.0	37.0	35.0	37.0
7	36.233596134776526	37.0	37.0	37.0	35.0	37.0
8	36.26242068782901	37.0	37.0	37.0	35.0	37.0
9	38.049013388042006	39.0	39.0	39.0	37.0	39.0
10	37.57512205008421	39.0	38.0	39.0	35.0	39.0
11	37.47383698131141	39.0	37.0	39.0	35.0	39.0
12	37.438760637401565	39.0	37.0	39.0	35.0	39.0
13	37.448340608064505	39.0	37.0	39.0	35.0	39.0
14	38.75611492858702	40.0	38.0	41.0	35.0	41.0
15	38.788033151342105	40.0	38.0	41.0	35.0	41.0
16	38.84757231569409	40.0	38.0	41.0	35.0	41.0
17	38.83168153838628	40.0	38.0	41.0	35.0	41.0
18	38.32285828989331	39.0	38.0	41.0	35.0	41.0
19	37.79835780806047	38.0	37.0	41.0	34.0	41.0
20	37.224490619984145	38.0	35.0	40.0	34.0	41.0
21	37.180546377478954	38.0	35.0	40.0	34.0	41.0
22	36.99151725707044	38.0	35.0	40.0	34.0	41.0
23	36.88334830729521	38.0	35.0	40.0	33.0	41.0
24	36.681785320754685	37.0	35.0	40.0	33.0	41.0
25	36.554058073087944	37.0	35.0	40.0	33.0	41.0
26	36.68174571805518	37.0	35.0	40.0	33.0	41.0
27	36.72421689544344	37.0	35.0	40.0	33.0	41.0
28	36.63309030735577	37.0	35.0	40.0	33.0	41.0
29	36.50125369722261	37.0	35.0	40.0	33.0	41.0
30	36.19247921440669	37.0	35.0	40.0	32.0	41.0
31	35.62501135665648	37.0	35.0	40.0	30.0	41.0
32	34.81193066266188	37.0	35.0	40.0	22.0	41.0
33	33.780230643015834	37.0	34.0	40.0	15.0	41.0
34	32.878556380654935	37.0	33.0	40.0	10.0	41.0
35	32.31801821879482	37.0	31.0	40.0	8.0	41.0
36	32.05872614422682	37.0	31.0	40.0	8.0	41.0
37	31.805687413563227	37.0	30.0	40.0	7.0	41.0
38	31.63508131366037	37.0	29.0	40.0	7.0	41.0
39	31.59447922838411	37.0	29.0	40.0	7.0	41.0
40	31.509449902973387	37.0	27.0	40.0	7.0	41.0
41	31.38190746790434	37.0	27.0	40.0	7.0	41.0
42	31.239770273281923	37.0	25.0	40.0	7.0	41.0
43	31.145493329274704	37.0	24.0	40.0	7.0	41.0
44	31.041291638161784	36.0	24.0	40.0	7.0	41.0
45	31.006936684601772	36.0	23.0	40.0	7.0	41.0
46	30.960630257549557	36.0	23.0	40.0	7.0	41.0
47	30.910542160956243	36.0	23.0	40.0	7.0	41.0
48	30.832535714257983	35.0	23.0	40.0	7.0	41.0
49	30.70434954119108	35.0	24.0	40.0	7.0	41.0
50	30.607664597749167	35.0	24.0	40.0	7.0	41.0
51	30.05465716098342	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	12.0
11	11.0
12	17.0
13	29.0
14	34.0
15	77.0
16	134.0
17	247.0
18	466.0
19	968.0
20	1597.0
21	2522.0
22	4233.0
23	7470.0
24	14912.0
25	32582.0
26	57220.0
27	68768.0
28	61245.0
29	47630.0
30	38367.0
31	33729.0
32	32764.0
33	37779.0
34	66006.0
35	83250.0
36	85622.0
37	116890.0
38	215450.0
39	277555.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.530328290848438	15.616742157694844	18.61660781912593	37.236321732330794
2	51.14906067832435	17.86338000498528	18.674614126049956	12.312945190640406
3	22.495886366654215	17.753812536351006	47.59304887206076	12.157252224934014
4	20.2416385888704	19.2649273057507	47.33136044591086	13.162073659468035
5	18.854301668516086	21.701891067727605	45.536193372992976	13.907613890763331
6	19.28488396020783	24.58232741182381	45.05878671306136	11.074001914907
7	58.78686836606251	4.447849068676517	33.28521475922723	3.480067806033743
8	60.16543057064384	3.7521616473480552	32.59861266307965	3.4837951189284593
9	55.793137240437304	5.509279067799045	34.218363072889936	4.479220618873715
10	29.14510196142076	20.66453329771679	40.160088088828076	10.030276652034376
11	22.26440470542192	18.350104947153692	45.74678655154447	13.638703795879922
12	19.66002247259066	16.423860704104936	49.395592918416106	14.520523904888293
13	17.651000822338407	18.487471957794394	49.92285238831457	13.938674831552635
14	14.90001094898163	20.644654295611634	48.7110874357718	15.74424731963494
15	14.752549132584402	19.467832901456834	51.65574227494989	14.123875691008866
16	16.632745530913013	18.678807353056513	49.5579639863922	15.130483129638272
17	16.52838076986095	18.81252470315447	47.799138214197804	16.85995631278678
18	16.851026292309854	19.232856884385743	48.89178445881358	15.024332364490823
19	16.85708317576377	20.656457453111567	47.34914283451274	15.13731653661192
20	17.963163277270926	20.379626818326887	47.56152201715962	14.095687887242573
21	17.672898785594867	20.774256071055007	47.56159966951159	13.991245473838534
22	16.506793416012382	19.350267240569316	46.8204079699268	17.322531373491508
23	15.710235589470653	19.953936624809458	47.58008092928122	16.755746856438662
24	16.653323404185926	18.63803986827055	48.54514435960493	16.163492367938588
25	15.69400624790824	20.31043857271871	46.5912558792537	17.40429930011935
26	15.555862713747807	20.731857886877606	47.524637149972314	16.18764224940227
27	16.361894127230272	20.134478343147297	47.4503238491339	16.053303680488526
28	14.436038145941382	20.31688371793249	48.72537546853488	16.52170266759125
29	16.59694779665334	19.301967477641945	47.305424560351796	16.795660165352917
30	18.43031982674207	19.992607496092145	45.99294450729971	15.584128169866073
31	18.41214917638033	21.028800480823364	44.76634795553005	15.792702387266258
32	18.97272150527531	23.018564347786246	42.45386091376629	15.554853233172153
33	19.370223895026445	23.602975948737022	40.39490880119522	16.631891355041304
34	18.657375303911895	23.87204134832438	39.853594255589606	17.61698909217412
35	18.99027093682127	25.96663589045117	37.040327195950276	18.002765976777287
36	20.47343085951059	27.312118193091887	35.682265212289884	16.53218573510764
37	19.49143921645671	28.026908093005776	36.39953998746691	16.082112703070607
38	18.576694510211674	28.69200048765677	35.252459444117875	17.478845558013685
39	20.24451172589341	27.38278183338756	34.77388799890665	17.598818441812377
40	21.12679774901362	25.924160053921792	35.25315831528563	17.695883881778954
41	19.13089934624485	27.228098348256818	33.85828911678991	19.782713188708417
42	18.913938674831552	27.04196566057691	34.4153670898461	19.628728574745434
43	19.549445523380733	25.945359146010492	34.84944373737664	19.655751593232132
44	18.774475050687574	25.433707798858666	35.27490097383815	20.516916176615617
45	19.57670149892335	24.854032991378258	34.601499777526016	20.967765732172378
46	20.502861100908454	25.144297483054316	35.120139836355435	19.232701579681795
47	18.454159098797867	25.28966268594826	37.20588201035727	19.050296204896604
48	18.234247638009585	24.46018026216987	37.13646080769317	20.169111292127372
49	18.9866989286305	23.40799089293216	38.45973453766954	19.145575640767795
50	18.4290773891105	23.43819765784976	37.57123632639147	20.56148862664827
51	17.956019260889384	23.05521625791763	36.06105338521546	22.92771109597753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	694.0
2	990.0
3	51694.5
4	102399.0
5	70305.5
6	38212.0
7	36147.0
8	34082.0
9	33217.0
10	32352.0
11	31449.5
12	30547.0
13	29225.0
14	27903.0
15	25978.0
16	24053.0
17	22230.0
18	20407.0
19	18810.5
20	17214.0
21	15696.0
22	14178.0
23	13303.5
24	12429.0
25	12419.5
26	12823.5
27	13237.0
28	14501.0
29	15765.0
30	16680.5
31	17596.0
32	19999.0
33	22402.0
34	23997.0
35	25592.0
36	28155.5
37	30719.0
38	33742.0
39	36765.0
40	40566.5
41	44368.0
42	50908.0
43	57448.0
44	62256.5
45	67065.0
46	76938.0
47	86811.0
48	93297.0
49	99783.0
50	98209.5
51	96636.0
52	83474.5
53	70313.0
54	61010.5
55	51708.0
56	47095.5
57	42483.0
58	39486.0
59	36489.0
60	34216.0
61	31943.0
62	29054.0
63	26165.0
64	22180.0
65	18195.0
66	14309.0
67	10423.0
68	8661.0
69	6899.0
70	5633.5
71	4368.0
72	3599.0
73	2830.0
74	2444.0
75	1572.0
76	1086.0
77	820.0
78	554.0
79	440.5
80	327.0
81	214.0
82	101.0
83	63.5
84	26.0
85	20.0
86	14.0
87	13.5
88	13.0
89	19.5
90	26.0
91	16.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1287791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.39727016189601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81783657276502	30.263897644606264
2	9.20988200184755	7.07268654768248
3	3.157232736202938	3.636873550078991
4	1.569428007849637	2.4104700486819506
5	0.9562707656890761	1.8359093469043304
6	0.6929749624970902	1.5965008110258316
7	0.5197477831999122	1.3969827233301508
8	0.41022193483106334	1.2601121966435231
9	0.36145451617766633	1.2490980038020139
>10	4.046936160969129	34.19345646890374
>50	0.19249250242273855	4.586513803536703
>100	0.058928641338050716	4.107035909208738
>500	0.00473901646354387	1.2834882557059781
>1k	0.0016483535525369985	1.1902903782960266
>5k	0.0	0.0
>10k+	2.0604419406712482E-4	3.9166843115934262
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49506	3.8442573367883455	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3523	0.2735692360018046	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3236	0.25128301098547823	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	1888	0.14660764052552006	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	1689	0.13115482248284077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.553047039465255E-4	0.0	0.0	0.1571683603938838	0.0
2	1.553047039465255E-4	0.0	0.0	0.8802670619689065	0.0
3	1.553047039465255E-4	0.0	0.0	1.2953188832659959	0.0
4	1.553047039465255E-4	0.0	0.0	1.7043914734611438	0.0
5	1.553047039465255E-4	0.0	0.0	2.755105448011362	0.0
6	1.553047039465255E-4	0.0	0.0	3.334469646083875	0.0
7	1.553047039465255E-4	0.0	0.0	3.9737038075277744	0.0
8	1.553047039465255E-4	0.0	0.0	4.933797487325195	0.0
9	1.553047039465255E-4	0.0	0.0	5.306606429148829	0.0
10	1.553047039465255E-4	0.0	0.0	6.1565890738481635	0.0
11	1.553047039465255E-4	0.0	0.0	7.310503024170847	0.0
12	1.553047039465255E-4	0.0	0.0	8.306394438227942	0.0
13	1.553047039465255E-4	0.0	0.0	8.670583968982545	0.0
14	1.553047039465255E-4	0.0	0.0	8.818356394787664	0.0
15	1.553047039465255E-4	0.0	0.0	9.08175317268097	0.0
16	1.553047039465255E-4	0.0	0.0	9.641393673352276	0.0
17	1.553047039465255E-4	0.0	0.0	10.375829618315395	0.0
18	1.553047039465255E-4	0.0	0.0	11.175105277176188	0.0
19	1.553047039465255E-4	0.0	0.0	11.682641049673434	0.0
20	1.553047039465255E-4	0.0	0.0	12.16548337424318	0.0
21	1.553047039465255E-4	0.0	0.0	12.746012357595292	0.0
22	1.553047039465255E-4	0.0	0.0	13.365056907526144	0.0
23	1.553047039465255E-4	0.0	0.0	13.9916337355984	0.0
24	1.553047039465255E-4	0.0	0.0	14.51283632204294	0.0
25	1.553047039465255E-4	0.0	0.0	14.956619513570137	0.0
26	1.553047039465255E-4	0.0	0.0	15.366158017877124	0.0
27	1.553047039465255E-4	0.0	0.0	15.777094264519631	0.0
28	2.3295705591978822E-4	0.0	0.0	16.191058952889094	0.0
29	2.3295705591978822E-4	0.0	0.0	16.627076909218964	0.0
30	2.3295705591978822E-4	0.0	0.0	17.116364379002494	0.0
31	2.3295705591978822E-4	0.0	0.0	17.574280298588825	0.0
32	3.882617598663137E-4	0.0	0.0	18.01355965370157	0.0
33	3.882617598663137E-4	0.0	0.0	18.428223213238795	0.0
34	3.882617598663137E-4	0.0	0.0	18.858960809634482	0.0
35	4.6591411183957644E-4	0.0	0.0	19.296065898891978	0.0
36	4.6591411183957644E-4	0.0	0.0	19.724629229432416	0.0
37	4.6591411183957644E-4	0.0	0.0	20.1576963963873	0.0
38	4.6591411183957644E-4	0.0	0.0	20.602644373194096	0.0
39	4.6591411183957644E-4	0.0	0.0	21.044253298866042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTGT	25	3.8910675E-5	45.000004	14
CAATGCG	25	3.8910675E-5	45.000004	1
TGTGTCG	20	7.033603E-4	45.0	1
CAATACG	20	7.033603E-4	45.0	11
TAGTGCG	60	0.0	44.999996	1
TAGTACG	30	2.165707E-6	44.999996	1
CGTTTTT	36315	0.0	44.566296	1
TTGTGCG	90	0.0	40.000004	1
CCGTGCG	45	1.9286745E-8	40.000004	1
GTTTTTT	40920	0.0	39.66642	2
CGCACGG	40	3.4586628E-7	39.375	2
GGTTCGT	40	3.4586628E-7	39.375	9
TAAAGCG	70	0.0	38.571426	1
ACGCATG	35	6.248876E-6	38.571426	1
ACGTATG	35	6.248876E-6	38.571426	1
CGGTATT	35	6.248876E-6	38.571426	43
TATGTCG	35	6.248876E-6	38.571426	1
CTCGTAG	30	1.1399985E-4	37.499996	1
TATTCGG	30	1.1399985E-4	37.499996	2
CCGCCGT	30	1.1399985E-4	37.499996	26
>>END_MODULE