##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275580
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64987770269211	34.0	31.0	34.0	31.0	34.0
2	32.80275874504147	34.0	31.0	34.0	31.0	34.0
3	32.84421439658822	34.0	31.0	34.0	31.0	34.0
4	36.234885307076	37.0	37.0	37.0	35.0	37.0
5	36.12434030009878	37.0	37.0	37.0	35.0	37.0
6	36.119211652738365	37.0	36.0	37.0	35.0	37.0
7	36.24775004311764	37.0	37.0	37.0	35.0	37.0
8	36.25867683720347	37.0	37.0	37.0	35.0	37.0
9	38.028545445993196	39.0	39.0	39.0	35.0	39.0
10	37.62705592749965	39.0	38.0	39.0	35.0	39.0
11	37.51813057589489	39.0	37.0	39.0	35.0	39.0
12	37.442521049248185	39.0	37.0	39.0	35.0	39.0
13	37.42582197902131	39.0	37.0	39.0	35.0	39.0
14	38.74882876808981	40.0	38.0	41.0	35.0	41.0
15	38.7943304222393	40.0	38.0	41.0	35.0	41.0
16	38.863128929584974	40.0	38.0	41.0	35.0	41.0
17	38.835702974333245	40.0	38.0	41.0	35.0	41.0
18	38.3096144498973	39.0	38.0	41.0	35.0	41.0
19	37.80492011477132	38.0	37.0	41.0	35.0	41.0
20	37.228729675912135	38.0	35.0	41.0	34.0	41.0
21	37.17331802003795	38.0	35.0	41.0	34.0	41.0
22	36.99736747205193	38.0	35.0	41.0	34.0	41.0
23	36.88468069427241	38.0	35.0	40.0	33.0	41.0
24	36.6992850311231	37.0	35.0	40.0	33.0	41.0
25	36.54517866382351	37.0	35.0	40.0	33.0	41.0
26	36.65351526364477	37.0	35.0	40.0	33.0	41.0
27	36.670227661142384	37.0	35.0	40.0	33.0	41.0
28	36.5819478198153	37.0	35.0	40.0	33.0	41.0
29	36.41831480581383	37.0	35.0	40.0	33.0	41.0
30	36.1020994371188	37.0	35.0	40.0	32.0	41.0
31	35.575613446432214	37.0	35.0	40.0	30.0	41.0
32	34.79563806268521	37.0	35.0	40.0	22.0	41.0
33	33.81932532651813	37.0	34.0	40.0	16.0	41.0
34	32.97374135687295	37.0	33.0	40.0	10.0	41.0
35	32.46123018548425	37.0	32.0	40.0	9.0	41.0
36	32.23468931780053	37.0	31.0	40.0	8.0	41.0
37	31.98433732106179	37.0	30.0	40.0	8.0	41.0
38	31.835060913466815	37.0	30.0	40.0	7.0	41.0
39	31.75114144154032	37.0	29.0	40.0	7.0	41.0
40	31.621060223584564	36.0	29.0	40.0	7.0	41.0
41	31.473164364445978	36.0	28.0	40.0	7.0	41.0
42	31.335023283525924	36.0	27.0	40.0	7.0	41.0
43	31.225408049671522	36.0	26.0	40.0	7.0	41.0
44	31.111865974693863	36.0	25.0	40.0	7.0	41.0
45	31.06914815221311	35.0	24.0	40.0	7.0	41.0
46	31.033684284795935	35.0	24.0	40.0	7.0	41.0
47	31.01390426315872	35.0	24.0	40.0	7.0	41.0
48	30.965371046896315	35.0	24.0	40.0	7.0	41.0
49	30.849750701641607	35.0	24.0	40.0	7.0	41.0
50	30.747731228147195	35.0	24.0	40.0	7.0	41.0
51	30.184918233274274	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	18.0
11	17.0
12	29.0
13	39.0
14	55.0
15	86.0
16	160.0
17	310.0
18	589.0
19	1114.0
20	1865.0
21	2881.0
22	4377.0
23	7285.0
24	13646.0
25	27886.0
26	51211.0
27	66815.0
28	63635.0
29	50332.0
30	39458.0
31	34255.0
32	32245.0
33	37316.0
34	67227.0
35	84477.0
36	86818.0
37	110675.0
38	202741.0
39	287816.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.803132692579066	15.323617491650857	17.850468022389816	38.02278179338027
2	50.7815268348516	18.05225858041048	18.467442261559448	12.698772323178476
3	23.24519042318004	17.98107527556092	46.249078850405304	12.52465545085373
4	21.091111494379028	18.536038507972844	46.83124539425202	13.5416046033961
5	19.350099562551936	21.69609118989009	44.94951316263974	14.004296084918233
6	19.84258141394503	25.24694648708823	43.66539142978097	11.245080669185782
7	59.28510951880713	4.32070117123191	32.99965505887518	3.3945342510857803
8	60.702895937534294	4.027579610843695	32.11503786512802	3.1544865864939866
9	56.421157434265204	5.354270214333871	33.49205851455808	4.732513836842847
10	31.10341962087835	19.20389156305367	38.68459837877671	11.008090437291271
11	24.934774769124633	18.780162749494348	43.192586901644745	13.092475579736277
12	22.643503347496825	16.486382665140564	45.99758541212625	14.872528575236363
13	18.92840903745747	17.349989808557677	47.37640916289061	16.345191991094246
14	15.335768826729801	19.728202072782576	47.47824519042318	17.457783910064443
15	15.165650135624578	18.146568619765127	50.742093792627664	15.945687451982627
16	17.625001959892757	17.692892644914473	48.61012245409931	16.071982941093463
17	17.364336223521846	17.855014973580648	46.96835949136863	17.812289311528872
18	17.820756048229043	18.655199987456687	46.89921447498393	16.624829489330345
19	19.19385691214977	19.68853384342809	45.4350961915364	15.682513052885746
20	21.032549898869533	18.884977814014018	45.80465356935669	14.277818717759763
21	19.50728296147635	19.417519873312532	45.7478166794713	15.32738048573982
22	18.519105034572508	18.38442120447169	45.79579485410558	17.300678906850216
23	18.226375452735226	19.15434547421565	46.64411483403628	15.975164239012841
24	18.705059659135454	17.591370200222645	46.81352796374982	16.89004217689208
25	17.44963075620502	18.967214913999904	45.62034525470766	17.96280907508741
26	17.08258204111071	19.799542169052508	46.386741717493216	16.73113407234356
27	17.381034509791622	19.596810862509603	46.19020367205506	16.831950955643705
28	16.154690415340472	20.405462613085813	46.67351322535631	16.766333746217406
29	17.916634001787422	19.67238432712962	45.62065883754841	16.79032283353455
30	18.273334483137084	19.294908982580473	45.765769297104065	16.66598723717838
31	19.18946675237931	20.216842534376518	43.56559369071324	17.02809702253093
32	20.073848758995908	21.943978425500557	41.51546747361984	16.466705341883692
33	20.13813324134903	22.78978974270528	40.0526035215353	17.019473494410388
34	19.7889587481773	24.08175104658273	38.64022640681102	17.48906379842895
35	19.357860737860424	25.24200755734646	36.67194531115258	18.728186393640538
36	20.690431019614607	27.556248922058984	34.51723921667006	17.236080841656346
37	20.589770927734836	27.903228335345492	34.818513930917696	16.688486806001976
38	20.418554696686996	26.910425061540632	34.058859499208204	18.612160742564164
39	21.47125229307452	26.40861412063532	33.70748992615124	18.412643660138915
40	21.241944840778313	25.14636479091864	35.20963012903934	18.402060239263708
41	18.882469151288042	26.810940905313664	34.277818717759764	20.028771225638533
42	20.011132190846517	26.376550275168942	34.023267846783426	19.589049687201115
43	20.86313676915599	26.39481647564245	33.13888584016683	19.603160915034728
44	20.230404992238824	26.256291255742486	33.294501324887506	20.218802427131187
45	19.849009862180342	25.330124335596356	33.57562834161715	21.245237460606155
46	19.723655121591747	25.964816005268194	34.420342118879255	19.891186754260808
47	18.895639630599415	25.630771884162503	36.55748757427993	18.916100910958153
48	19.1581868640148	23.934523902852035	36.80780507690619	20.09948415622697
49	19.320387588391164	23.75938788629486	37.240941375687925	19.679283149626052
50	18.47943680521802	23.68812618573512	37.06196396933159	20.770473039715267
51	17.853525455087098	23.521064927327178	36.427899465341255	22.19751015224447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	745.0
2	1113.0
3	53021.5
4	104930.0
5	72995.0
6	41060.0
7	38395.5
8	35731.0
9	34371.5
10	33012.0
11	31282.5
12	29553.0
13	27726.0
14	25899.0
15	23769.0
16	21639.0
17	20084.0
18	18529.0
19	16933.5
20	15338.0
21	13880.5
22	12423.0
23	11594.0
24	10765.0
25	10800.5
26	10811.5
27	10787.0
28	12036.5
29	13286.0
30	14781.0
31	16276.0
32	17447.5
33	18619.0
34	20585.5
35	22552.0
36	24384.5
37	26217.0
38	28907.0
39	31597.0
40	35371.0
41	39145.0
42	43555.5
43	47966.0
44	52160.0
45	56354.0
46	62822.5
47	69291.0
48	72280.5
49	75270.0
50	76448.0
51	77626.0
52	71843.0
53	66060.0
54	61766.0
55	57472.0
56	55273.0
57	53074.0
58	51799.0
59	50524.0
60	50965.0
61	51406.0
62	47636.5
63	43867.0
64	37165.5
65	30464.0
66	25331.5
67	20199.0
68	16448.0
69	12697.0
70	10904.0
71	9111.0
72	7615.0
73	6119.0
74	4815.0
75	2874.5
76	2238.0
77	1873.0
78	1508.0
79	1034.0
80	560.0
81	448.5
82	337.0
83	241.5
84	146.0
85	106.0
86	66.0
87	39.0
88	12.0
89	10.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1275580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.2724381840111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40754061065189	27.21490026922918
2	8.273881861915939	5.671322093086493
3	2.749687481948111	2.827154827512473
4	1.327428929767547	1.8197690375650506
5	0.8485148885381498	1.4540337032818407
6	0.571662946127669	1.1755369789950123
7	0.4598089199793928	1.1031140950515455
8	0.3904109735912579	1.0704268767012783
9	0.3466869412068242	1.0693626085543295
>10	5.344359831459283	42.35750982492287
>50	0.20698330955883823	4.381731085213116
>100	0.06626664121661173	4.06490381075159
>500	0.0041999983687512335	0.9655456018012064
>1k	0.0023333324270840184	1.0640673991690286
>5k	0.0	0.0
>10k+	2.3333324270840186E-4	3.760621788164944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47026	3.6866366672415682	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1954	0.1531852177048872	RNA PCR Primer, Index 17 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1833	0.14369933677229182	RNA PCR Primer, Index 47 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	1476	0.11571206823562616	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1320	0.10348233744649493	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	1279	0.10026811332883864	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.839571018673858E-5	0.0	0.0	0.09164458520829741	0.0
2	7.839571018673858E-5	0.0	0.0	0.5076122234591323	0.0
3	7.839571018673858E-5	0.0	0.0	0.7518932564010098	0.0
4	7.839571018673858E-5	0.0	0.0	0.9838661628435692	0.0
5	7.839571018673858E-5	0.0	0.0	1.6471722667335644	0.0
6	7.839571018673858E-5	0.0	0.0	2.01672964455385	0.0
7	7.839571018673858E-5	0.0	0.0	2.4022013515420437	0.0
8	7.839571018673858E-5	0.0	0.0	2.9838191254174573	0.0
9	7.839571018673858E-5	0.0	0.0	3.2137537433951615	0.0
10	7.839571018673858E-5	0.0	0.0	3.7955283086909484	0.0
11	7.839571018673858E-5	0.0	0.0	4.473259223255304	0.0
12	7.839571018673858E-5	0.0	0.0	5.12739302905345	0.0
13	7.839571018673858E-5	0.0	0.0	5.357249251320968	0.0
14	7.839571018673858E-5	0.0	0.0	5.4528920177487885	0.0
15	7.839571018673858E-5	0.0	0.0	5.640022577964534	0.0
16	7.839571018673858E-5	0.0	0.0	6.005033004593988	0.0
17	7.839571018673858E-5	0.0	0.0	6.454867589645494	0.0
18	7.839571018673858E-5	0.0	0.0	6.9192053810815475	0.0
19	7.839571018673858E-5	0.0	0.0	7.257718057667884	0.0
20	7.839571018673858E-5	0.0	0.0	7.565421220150833	0.0
21	7.839571018673858E-5	0.0	0.0	7.939839132002697	0.0
22	1.5679142037347717E-4	0.0	0.0	8.338010944041143	0.0
23	1.5679142037347717E-4	0.0	0.0	8.738221044544442	0.0
24	1.5679142037347717E-4	0.0	0.0	9.0553316922498	0.0
25	1.5679142037347717E-4	0.0	0.0	9.348923626899136	0.0
26	1.5679142037347717E-4	0.0	0.0	9.625582088148137	0.0
27	1.5679142037347717E-4	0.0	0.0	9.884758306025494	0.0
28	1.5679142037347717E-4	0.0	0.0	10.154674736198436	0.0
29	1.5679142037347717E-4	0.0	0.0	10.41463491117766	0.0
30	1.5679142037347717E-4	0.0	0.0	10.736841280045155	0.0
31	2.3518713056021575E-4	0.0	0.0	11.03623449724831	0.0
32	2.3518713056021575E-4	0.0	0.0	11.32614183351887	0.0
33	2.3518713056021575E-4	0.0	0.0	11.615422004107936	0.0
34	2.3518713056021575E-4	0.0	0.0	11.89537308518478	0.0
35	2.3518713056021575E-4	0.0	0.0	12.189200206964674	0.0
36	3.1358284074695434E-4	0.0	0.0	12.497295347998557	0.0
37	3.1358284074695434E-4	0.0	0.0	12.78641872716725	0.0
38	3.1358284074695434E-4	0.0	0.0	13.09482745104188	0.0
39	3.1358284074695434E-4	0.0	0.0	13.416641841358441	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	20	7.033579E-4	45.0	11
TATAGCG	20	7.033579E-4	45.0	1
TCGGTAA	25	3.891047E-5	45.0	33
CTATGCG	30	2.1656906E-6	44.999996	1
ACGTTAG	30	2.1656906E-6	44.999996	1
TATACGG	30	2.1656906E-6	44.999996	2
CGTTTTT	35870	0.0	44.43546	1
CAGTACG	55	6.184564E-11	40.909092	1
GTACGAG	95	0.0	40.263157	1
GTTTTTT	39785	0.0	40.192913	2
ACTAACG	40	3.4586265E-7	39.375	1
AACGTAG	30	1.13999224E-4	37.499996	1
TACCGGC	30	1.13999224E-4	37.499996	25
TAACGGG	285	0.0	37.10526	3
ACAACGA	165	0.0	36.81818	13
CACAACG	165	0.0	36.81818	12
ACCGGGT	50	4.879621E-8	36.0	4
TACGTCC	25	0.002107195	36.0	33
GTTGATC	140	0.0	35.357143	16
TCGACAC	250	0.0	35.100002	34
>>END_MODULE