##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934910.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131094
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56544283675804	34.0	31.0	34.0	31.0	34.0
2	32.72319895605494	34.0	31.0	34.0	31.0	34.0
3	32.78319220153232	34.0	31.0	34.0	31.0	34.0
4	36.1849722481067	37.0	37.0	37.0	35.0	37.0
5	36.06578940388685	37.0	35.0	37.0	35.0	37.0
6	36.045957276760376	37.0	35.0	37.0	35.0	37.0
7	36.1984308996423	37.0	37.0	37.0	35.0	37.0
8	36.2088880322944	37.0	37.0	37.0	35.0	37.0
9	37.96482697282454	39.0	39.0	39.0	35.0	39.0
10	37.55268262407899	39.0	37.0	39.0	35.0	39.0
11	37.43586209457393	39.0	37.0	39.0	34.0	39.0
12	37.38610672499368	39.0	37.0	39.0	35.0	39.0
13	37.357491950271154	39.0	37.0	39.0	34.0	39.0
14	38.64542204273031	40.0	38.0	41.0	35.0	41.0
15	38.68420484946432	40.0	38.0	41.0	35.0	41.0
16	38.77323900577671	40.0	38.0	41.0	35.0	41.0
17	38.793130367591026	40.0	38.0	41.0	35.0	41.0
18	38.31565015816546	39.0	38.0	41.0	35.0	41.0
19	37.8425029219499	38.0	37.0	41.0	35.0	41.0
20	37.28361214894606	38.0	35.0	41.0	34.0	41.0
21	37.2224527758082	38.0	35.0	41.0	34.0	41.0
22	37.028031268842376	38.0	35.0	40.0	34.0	41.0
23	36.92179606646309	38.0	35.0	40.0	33.0	41.0
24	36.71214770832486	37.0	35.0	40.0	33.0	41.0
25	36.54547721055898	37.0	35.0	40.0	33.0	41.0
26	36.67132793560924	38.0	35.0	40.0	33.0	41.0
27	36.721102755385495	38.0	35.0	40.0	33.0	41.0
28	36.66903281248066	38.0	35.0	40.0	33.0	41.0
29	36.561693369428184	38.0	35.0	40.0	33.0	41.0
30	36.29896012179359	38.0	35.0	40.0	33.0	41.0
31	35.82659796621678	38.0	35.0	40.0	31.0	41.0
32	35.14702756800054	38.0	35.0	40.0	24.0	41.0
33	34.25635800384406	38.0	34.0	40.0	18.0	41.0
34	33.46480398622926	38.0	33.0	40.0	12.0	41.0
35	32.960260597262476	38.0	33.0	40.0	10.0	41.0
36	32.721612880980715	38.0	33.0	40.0	10.0	41.0
37	32.461512482605336	37.0	32.0	40.0	8.0	41.0
38	32.30630699128454	37.0	31.0	40.0	8.0	41.0
39	32.21384960047529	37.0	31.0	40.0	8.0	41.0
40	32.07786974380556	37.0	31.0	40.0	8.0	41.0
41	31.943305330945083	37.0	30.0	40.0	8.0	41.0
42	31.801315363709826	37.0	30.0	40.0	7.0	41.0
43	31.707298420820905	37.0	29.0	40.0	7.0	41.0
44	31.60189780867019	36.0	29.0	40.0	7.0	41.0
45	31.558047341777076	36.0	29.0	40.0	7.0	41.0
46	31.50078154423947	36.0	28.0	40.0	7.0	41.0
47	31.478803706853718	36.0	28.0	40.0	7.0	41.0
48	31.419378937559568	36.0	28.0	40.0	7.0	41.0
49	31.31831129861886	36.0	27.0	40.0	7.0	41.0
50	31.24495311618663	35.0	27.0	40.0	7.0	41.0
51	30.70098329581803	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	9.0
11	14.0
12	12.0
13	18.0
14	31.0
15	74.0
16	106.0
17	242.0
18	492.0
19	812.0
20	1457.0
21	2130.0
22	3540.0
23	5907.0
24	11250.0
25	23853.0
26	42445.0
27	55217.0
28	51436.0
29	41631.0
30	32362.0
31	28097.0
32	27811.0
33	34097.0
34	59028.0
35	82900.0
36	80969.0
37	105352.0
38	190408.0
39	249237.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.799026429279973	16.17451776775405	17.567682261598065	36.45877354136792
2	49.879585604733116	18.710734916815046	17.95518321200537	13.454496266446467
3	23.248200414819635	18.472027965845456	44.88176933128457	13.398002288050332
4	20.99728227715822	19.77439540833918	45.177589130523195	14.050733183979405
5	18.752552838225647	22.94751806657979	43.852411912714594	14.447517182479972
6	19.775898378030472	25.67337462668885	42.96247703550722	11.588249959773458
7	60.281373608205854	4.963513200494388	31.079026146368026	3.676087044931721
8	61.24946290936032	3.9483013790188966	30.87011335927872	3.932122352342069
9	56.17181242230973	6.394693986529855	32.475903859449346	4.957589731711069
10	30.568900551147827	20.835049960480738	37.74487354720297	10.851175941168462
11	23.69387513327805	18.767317305193025	42.92950011228068	14.609307449248249
12	20.804371696782052	17.488113277941533	46.836337209816335	14.871177815460076
13	17.952884552477514	19.401924154844778	47.72804028666052	14.917151006017185
14	15.340281179106247	21.655848231888772	46.643161399494645	16.360709189510334
15	14.806638528716446	19.242874597513556	50.505970326073694	15.444516547696303
16	16.590928782223227	18.756442877426633	48.12111106592379	16.531517274426353
17	16.78269003283547	18.406427759319737	45.64430542465966	19.16657678318513
18	17.178324701572105	18.94051245961874	47.33337812772413	16.54778471108502
19	18.62382790466575	19.62869575826589	45.0854659294453	16.66201040762306
20	19.120692002609864	19.75715546188027	45.424871849731325	15.697280685778548
21	18.530024913932884	20.46531941642339	45.42036294065745	15.584292728986274
22	17.44417351696676	18.602432689060326	44.96823429352468	18.98515950044824
23	16.302977471368425	20.153762640417153	45.46235768203174	18.080902206182685
24	17.034304841153787	18.623297444774707	46.64484118914962	17.69755652492189
25	16.40712442997664	20.090814733346654	45.25477104467003	18.247289792006676
26	15.374407432096712	20.93910850910711	45.36758218149862	18.318901877297556
27	16.732384753168173	20.2989318305994	45.04161457845237	17.927068837780062
28	14.673669916028198	19.929378106505737	46.911397284399	18.485554693067066
29	15.649362475620949	18.954392826767712	45.90476123116204	19.491483466449296
30	16.43249809476489	19.63594537677682	44.0966002825583	19.83495624589999
31	17.43674707849215	21.159691413799383	41.50459643495589	19.898965072752574
32	17.677841098971438	21.739926124619174	41.3978855868743	19.184347189535085
33	17.373003481585084	22.652670777141424	38.980491453407055	20.993834287866438
34	16.38599444431674	23.561613800444526	38.50458052115916	21.54781123407957
35	16.83246485261172	24.28135946260877	36.68731334442584	22.198862340353674
36	18.720371604835673	25.945412140812348	34.42817307845325	20.90604317589873
37	17.95712823160586	27.01588020093821	35.36558411590902	19.661407451546907
38	17.679432478644568	27.649249310844194	34.04747969664767	20.623838513863568
39	18.51313860740133	26.33989747978506	34.58501238623845	20.561951526575157
40	18.792779379963115	26.188451180892123	34.227836059602474	20.790933379542285
41	17.171163493043018	27.065301380787098	32.98903539405213	22.774499732117757
42	17.89541806428113	26.54589273747363	33.203871649924764	22.354817548320476
43	18.752376018261966	25.078552268865362	34.16621430225958	22.00285741061309
44	18.11847644846494	24.604144306308758	34.16860137176928	23.108777873457026
45	18.08576475518392	24.917999741842852	33.71594226474546	23.28029323822777
46	18.71391767616131	25.272170129096256	34.22067485107338	21.793237343669052
47	17.15330467671122	24.60264133661747	36.514029780018284	21.730024206653027
48	17.005483187073754	24.048222340495133	36.09664625574886	22.849648216682255
49	17.41110818375838	23.056527574189236	37.54082330911489	21.991540932937493
50	17.129433981614262	23.1113417629304	37.143774080668805	22.615450174786535
51	17.008665946420017	23.332189897568195	34.99894792121609	24.66019623479569
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	619.5
2	960.0
3	41845.5
4	82731.0
5	57505.5
6	32280.0
7	30293.0
8	28306.0
9	27526.0
10	26746.0
11	26036.0
12	25326.0
13	23930.5
14	22535.0
15	20722.5
16	18910.0
17	17176.5
18	15443.0
19	14252.0
20	13061.0
21	12143.5
22	11226.0
23	10298.5
24	9371.0
25	9697.0
26	10409.5
27	10796.0
28	11644.0
29	12492.0
30	13225.0
31	13958.0
32	15860.0
33	17762.0
34	19671.0
35	21580.0
36	23394.5
37	25209.0
38	26208.0
39	27207.0
40	32063.0
41	36919.0
42	42087.0
43	47255.0
44	53322.0
45	59389.0
46	66880.0
47	74371.0
48	80715.0
49	87059.0
50	86615.0
51	86171.0
52	73654.5
53	61138.0
54	54418.5
55	47699.0
56	45163.5
57	42628.0
58	38523.5
59	34419.0
60	33766.0
61	33113.0
62	29974.5
63	26836.0
64	23830.5
65	20825.0
66	17760.0
67	14695.0
68	12787.5
69	10880.0
70	9152.5
71	7425.0
72	6677.0
73	5929.0
74	4700.0
75	2868.0
76	2265.0
77	1732.0
78	1199.0
79	957.0
80	715.0
81	510.0
82	305.0
83	222.0
84	139.0
85	84.0
86	29.0
87	17.5
88	6.0
89	5.0
90	4.0
91	5.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1131094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.00683882395536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26767329496256	26.616361508677876
2	9.291605994318799	6.319562949289938
3	3.396352218549788	3.4649760745681752
4	1.64542457854828	2.2382275375866416
5	0.9529649297012731	1.6203662384616575
6	0.6666276286123333	1.3601938993089118
7	0.47316484633360306	1.1263588466499914
8	0.3504947540359352	0.9535374887313544
9	0.299473559786449	0.9165734171724539
>10	4.031977490329116	33.93649600784369
>50	0.5463773281457324	11.562141099772921
>100	0.07254847041886173	4.204899256681259
>500	0.0034546890672515294	0.8090063862045228
>1k	0.0015944718771930136	0.9545950019524548
>5k	0.0	0.0
>10k+	2.657453128655023E-4	3.916704287098304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43340	3.831688612971159	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	3143	0.2778725729249735	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2757	0.24374631993450585	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	1386	0.12253623483105738	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1139	0.1006989693164317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5363992736235894E-4	0.0	0.0	0.13128882303327574	0.0
2	3.5363992736235894E-4	0.0	0.0	0.8706615011661276	0.0
3	3.5363992736235894E-4	0.0	0.0	1.2568363018458235	0.0
4	3.5363992736235894E-4	0.0	0.0	1.656891469674492	0.0
5	3.5363992736235894E-4	0.0	0.0	2.7215244709988737	0.0
6	3.5363992736235894E-4	0.0	0.0	3.3197064081323036	0.0
7	3.5363992736235894E-4	0.0	0.0	3.947417279200491	0.0
8	3.5363992736235894E-4	0.0	0.0	4.971646918823723	0.0
9	3.5363992736235894E-4	0.0	0.0	5.376122585744421	0.0
10	3.5363992736235894E-4	0.0	0.0	6.173315392001019	0.0
11	3.5363992736235894E-4	0.0	0.0	7.258017459203214	0.0
12	3.5363992736235894E-4	0.0	0.0	8.14485798704617	0.0
13	3.5363992736235894E-4	0.0	0.0	8.48355662747747	0.0
14	3.5363992736235894E-4	0.0	0.0	8.614757040528904	0.0
15	3.5363992736235894E-4	0.0	0.0	8.871941677703179	0.0
16	3.5363992736235894E-4	0.0	0.0	9.42671431375288	0.0
17	3.5363992736235894E-4	0.0	0.0	10.114455562490827	0.0
18	4.4204990920294863E-4	0.0	0.0	10.914742718111846	0.0
19	4.4204990920294863E-4	0.0	0.0	11.324876623870342	0.0
20	4.4204990920294863E-4	0.0	0.0	11.730324800591285	0.0
21	4.4204990920294863E-4	0.0	0.0	12.234173287100807	0.0
22	4.4204990920294863E-4	0.0	0.0	12.767639117526926	0.0
23	4.4204990920294863E-4	0.0	0.0	13.311537325810233	0.0
24	4.4204990920294863E-4	0.0	0.0	13.73687774844531	0.0
25	4.4204990920294863E-4	0.0	0.0	14.124555518816296	0.0
26	4.4204990920294863E-4	0.0	0.0	14.47748816632393	0.0
27	4.4204990920294863E-4	0.0	0.0	14.8206957158291	0.0
28	4.4204990920294863E-4	0.0	0.0	15.195465628851359	0.0
29	4.4204990920294863E-4	0.0	0.0	15.561836593598764	0.0
30	5.304598910435384E-4	0.0	0.0	15.984701536742305	0.0
31	5.304598910435384E-4	0.0	0.0	16.363980358838436	0.0
32	5.304598910435384E-4	0.0	0.0	16.723366935020433	0.0
33	5.304598910435384E-4	0.0	0.0	17.086643550403416	0.0
34	6.188698728841281E-4	0.0	0.0	17.449301295913514	0.0
35	6.188698728841281E-4	0.0	0.0	17.83282379713799	0.0
36	6.188698728841281E-4	0.0	0.0	18.190265353719496	0.0
37	6.188698728841281E-4	0.0	0.0	18.542048671463203	0.0
38	6.188698728841281E-4	0.0	0.0	18.89117968975169	0.0
39	6.188698728841281E-4	0.0	0.0	19.227314440709613	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGA	20	7.03324E-4	45.0	14
ATTGTCG	20	7.03324E-4	45.0	12
TCAATCG	20	7.03324E-4	45.0	39
TCTACGT	20	7.03324E-4	45.0	32
ACGTGCA	20	7.03324E-4	45.0	35
CGTAAAG	20	7.03324E-4	45.0	1
CGGTCCA	20	7.03324E-4	45.0	27
TATACGG	50	2.1827873E-11	45.0	2
CGATATA	20	7.03324E-4	45.0	10
CGTTTTT	29355	0.0	44.40214	1
GAGCACG	75	0.0	42.0	1
GTTTTTT	32780	0.0	39.865772	2
TTTAGCG	40	3.4581535E-7	39.375	1
TGTTACG	40	3.4581535E-7	39.375	1
TATAGCG	40	3.4581535E-7	39.375	1
ACGAAGG	160	0.0	39.375	2
CTCAACG	35	6.2482013E-6	38.571426	1
AGATACG	35	6.2482013E-6	38.571426	1
TCGACAG	35	6.2482013E-6	38.571426	1
GTAAGCG	65	9.094947E-12	38.076927	1
>>END_MODULE