##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934909.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1044871
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60247820065826	34.0	31.0	34.0	31.0	34.0
2	32.76734927086693	34.0	31.0	34.0	31.0	34.0
3	32.81382199333698	34.0	31.0	34.0	31.0	34.0
4	36.20577181297978	37.0	37.0	37.0	35.0	37.0
5	36.096247287942724	37.0	35.0	37.0	35.0	37.0
6	36.090682007635394	37.0	36.0	37.0	35.0	37.0
7	36.22533499350637	37.0	37.0	37.0	35.0	37.0
8	36.23594108746438	37.0	37.0	37.0	35.0	37.0
9	38.03302417236195	39.0	39.0	39.0	35.0	39.0
10	37.669923847058634	39.0	38.0	39.0	35.0	39.0
11	37.52411350300659	39.0	37.0	39.0	35.0	39.0
12	37.39177180723745	39.0	37.0	39.0	35.0	39.0
13	37.31126521838581	39.0	37.0	39.0	34.0	39.0
14	38.57467094024047	40.0	38.0	41.0	34.0	41.0
15	38.63133630850124	40.0	38.0	41.0	35.0	41.0
16	38.703118375378395	40.0	38.0	41.0	35.0	41.0
17	38.68607895137294	40.0	38.0	41.0	34.0	41.0
18	38.204843468715275	39.0	38.0	41.0	34.0	41.0
19	37.697160702134525	38.0	37.0	41.0	34.0	41.0
20	37.13866017910345	38.0	35.0	40.0	34.0	41.0
21	37.0837845054557	38.0	35.0	40.0	34.0	41.0
22	36.897996020561386	37.0	35.0	40.0	34.0	41.0
23	36.79649640960463	37.0	35.0	40.0	33.0	41.0
24	36.6117271892894	37.0	35.0	40.0	33.0	41.0
25	36.45186152166153	36.0	35.0	40.0	33.0	41.0
26	36.544803138377844	37.0	35.0	40.0	33.0	41.0
27	36.58166127684662	37.0	35.0	40.0	33.0	41.0
28	36.504758003619585	37.0	35.0	40.0	33.0	41.0
29	36.37370833337321	37.0	35.0	40.0	33.0	41.0
30	36.06426726361436	37.0	35.0	40.0	32.0	41.0
31	35.563611201765575	36.0	35.0	40.0	30.0	41.0
32	34.85328715219391	36.0	35.0	40.0	23.0	41.0
33	33.93726881117382	36.0	34.0	40.0	18.0	41.0
34	33.12834790131988	36.0	33.0	40.0	12.0	41.0
35	32.60228104713405	36.0	33.0	40.0	10.0	41.0
36	32.371234343761095	36.0	32.0	40.0	10.0	41.0
37	32.1160449471753	36.0	31.0	40.0	8.0	41.0
38	31.988251181246298	36.0	31.0	40.0	8.0	41.0
39	31.937229571880167	36.0	31.0	40.0	8.0	41.0
40	31.80030262108911	35.0	30.0	40.0	8.0	41.0
41	31.654916252819724	35.0	30.0	40.0	8.0	41.0
42	31.51616515340171	35.0	29.0	40.0	7.0	41.0
43	31.395047809729622	35.0	29.0	40.0	7.0	41.0
44	31.282138177822908	35.0	28.0	40.0	7.0	41.0
45	31.240102366703642	35.0	27.0	40.0	7.0	41.0
46	31.184114594050367	35.0	27.0	40.0	7.0	41.0
47	31.155774253472437	35.0	27.0	40.0	7.0	41.0
48	31.107170167417795	35.0	27.0	40.0	7.0	41.0
49	30.99324031387607	35.0	27.0	40.0	7.0	41.0
50	30.879165944886978	35.0	26.0	40.0	7.0	41.0
51	30.341111965017692	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	3.0
11	10.0
12	18.0
13	31.0
14	31.0
15	64.0
16	96.0
17	178.0
18	366.0
19	773.0
20	1228.0
21	1978.0
22	3239.0
23	5553.0
24	10115.0
25	20673.0
26	38013.0
27	50438.0
28	48808.0
29	40875.0
30	33725.0
31	29225.0
32	28561.0
33	34631.0
34	63994.0
35	86211.0
36	78117.0
37	96224.0
38	162600.0
39	208921.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.67071628937926	16.129550920639964	17.924030813373136	37.27570197660764
2	50.564997975826685	18.84117752334977	17.8252626400771	12.768561860746447
3	22.977669013686857	18.718674362672523	45.537295991562594	12.766360632078028
4	21.084133830874816	19.691043200548204	45.73492804374894	13.489894924828041
5	18.76241181925807	22.562976673675507	44.307861927453246	14.366749579613177
6	19.725592920082956	24.96384721176107	43.60959391159292	11.700965956563058
7	58.37926404312112	5.059476241564749	32.65637576313248	3.904883952181657
8	59.51270539616852	4.657512745592518	31.839432810366063	3.990349047872895
9	55.21954384799655	5.894507551649917	33.549787485727904	5.336161114625633
10	33.07499203250928	18.037441942593873	38.39861571428435	10.488950310612505
11	25.67867229543169	17.81119391771807	42.28876100494702	14.221372781903222
12	23.34192450551312	16.090598743768368	46.33471500309608	14.232761747622433
13	18.898313763134396	18.777246186371332	47.12447756708723	15.199962483407042
14	15.094016390540077	20.83520358015487	47.55266439589193	16.518115633413117
15	15.450902551606848	17.749559515002332	51.38509921320431	15.41443872018651
16	17.037797010348648	17.50445748805355	49.15659445041541	16.301151051182394
17	16.690672819898342	17.463208376919255	47.146968381742816	18.699150421439583
18	17.79128715410802	18.106062853691988	47.250617540347086	16.852032451852907
19	18.694460847319906	19.876616347855382	45.260993940878826	16.167928863945885
20	20.389119805219973	19.205433015176034	45.26089823528455	15.14454894431944
21	18.43777844346336	20.890999941619587	45.46475115109904	15.206470463818022
22	17.240214342248947	18.856873240811545	45.80756858980678	18.095343827132726
23	17.52914953137756	18.82787444574498	46.17402531030147	17.468950712576003
24	18.06931190548881	17.566091890769293	46.99718912669602	17.367407077045875
25	16.61075864867529	19.3439190100979	45.42388486234186	18.621437478884953
26	15.318733125907407	20.744857499155398	46.20139711026528	17.735012264671905
27	15.936608442573293	20.468938270848746	45.81455509818915	17.779898188388806
28	14.194575215505074	22.058703897418916	46.92713263168372	16.819588255392294
29	16.13960000803927	20.251973688618023	45.335070070850854	18.273356232491857
30	16.04580852564575	22.077940721869016	44.41390372591449	17.462347026570743
31	18.24971695070492	22.601546028169984	41.30060074401529	17.848136277109806
32	18.756669483601325	22.45482935214012	40.02331388276639	18.765187281492164
33	18.942816864474178	23.965350746647193	38.792157117960016	18.299675270918613
34	17.46694089509614	24.386263950286686	38.52006611342453	19.626729041192643
35	16.88466805950208	25.99794615794677	36.170302362684005	20.94708341986714
36	18.76336887520086	28.87724896183357	34.17914747370728	18.180234689258292
37	19.320471139499517	28.127491336251076	34.6736582793474	17.878379244902
38	17.981454169940594	28.232001845203857	33.413502719474465	20.373041265381087
39	20.05472445880879	25.753418364563665	34.1300505038421	20.061806672785444
40	18.827491623367862	25.70384286672709	34.63001652835614	20.838648981548918
41	16.81882261063806	26.795173758291696	33.43599353413005	22.950010096940197
42	17.968533914712918	25.612731140973384	33.57974333673726	22.838991607576435
43	19.37234357159879	24.905658210439373	34.46234032717915	21.25965789078269
44	17.67624902978454	25.661540994055727	34.20431804500269	22.45789193115705
45	17.86153506030888	24.89982016918835	33.86666870838601	23.371976062116758
46	17.834163260345058	26.137197797622864	35.007670803381465	21.02096813865061
47	16.81652567637536	24.437370737631728	37.37370450514944	21.372399080843472
48	16.970898799947555	23.01681260174701	37.31407991991355	22.69820867839188
49	17.615380271823028	22.787789114637118	38.73980615788935	20.857024455650507
50	16.700243379326253	22.732471281143795	37.866301198903976	22.70098414062597
51	15.834203456694654	22.55809568836727	36.338552797426665	25.269148057511405
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	886.0
2	1378.0
3	48938.5
4	96499.0
5	64438.0
6	32377.0
7	29860.0
8	27343.0
9	26093.0
10	24843.0
11	23808.5
12	22774.0
13	21144.5
14	19515.0
15	17757.5
16	16000.0
17	14467.5
18	12935.0
19	11990.5
20	11046.0
21	10032.5
22	9019.0
23	8251.0
24	7483.0
25	8076.5
26	9130.5
27	9591.0
28	10295.5
29	11000.0
30	12181.5
31	13363.0
32	14337.0
33	15311.0
34	16056.0
35	16801.0
36	18059.0
37	19317.0
38	21091.5
39	22866.0
40	26614.5
41	30363.0
42	35313.0
43	40263.0
44	46871.5
45	53480.0
46	59157.0
47	64834.0
48	70401.0
49	75968.0
50	73792.0
51	71616.0
52	61933.5
53	52251.0
54	47492.5
55	42734.0
56	40725.5
57	38717.0
58	37778.5
59	36840.0
60	36167.5
61	35495.0
62	33322.5
63	31150.0
64	27631.5
65	24113.0
66	20497.0
67	16881.0
68	14260.0
69	11639.0
70	9740.5
71	7842.0
72	6435.0
73	5028.0
74	3975.5
75	2542.0
76	2161.0
77	1677.5
78	1194.0
79	832.0
80	470.0
81	351.0
82	232.0
83	143.5
84	55.0
85	54.0
86	53.0
87	42.5
88	32.0
89	21.0
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1044871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.077897006300496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49390554956076	24.85841520834789
2	9.821847212437746	6.301284065843952
3	3.614446499595651	3.4783152764643797
4	1.7582496024320844	2.2560379863274083
5	1.0416566245383336	1.6707076958935647
6	0.6653835928435681	1.2806463816550901
7	0.466854523995184	1.048299793635007
8	0.3698721423807722	0.94917763910321
9	0.25657005670486616	0.7407205068491438
>10	3.642088381019809	29.61023917691658
>50	0.7779439054378562	15.771610736999625
>100	0.08203322252626867	4.646716039391024
>500	0.0060991243513904055	1.3483996944682133
>1k	0.0027446059581256827	1.3446823998955353
>5k	0.0	0.0
>10k+	3.049562175695203E-4	4.694747398209408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47992	4.593102880642682	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2825	0.2703683038384643	No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2632	0.2518971241425975	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	1591	0.1522676004980519	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1413	0.1352320047163717	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1132	0.10833873272394393	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1078	0.10317063063287238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16231668789735768	0.0
2	0.0	0.0	0.0	0.9133184862054742	0.0
3	0.0	0.0	0.0	1.3015003766015134	0.0
4	0.0	0.0	0.0	1.7093976194190479	0.0
5	0.0	0.0	0.0	2.7636904459976397	0.0
6	0.0	0.0	0.0	3.3184957760335965	0.0
7	0.0	0.0	0.0	3.9185698521635683	0.0
8	0.0	0.0	0.0	4.7844183636066075	0.0
9	0.0	0.0	0.0	5.144749926067428	0.0
10	0.0	0.0	0.0	5.966191041764964	0.0
11	0.0	0.0	0.0	7.036466702588166	0.0
12	0.0	0.0	0.0	7.981272329311465	0.0
13	0.0	0.0	0.0	8.324951118367721	0.0
14	0.0	0.0	0.0	8.467552453843584	0.0
15	0.0	0.0	0.0	8.739356341596235	0.0
16	0.0	0.0	0.0	9.306986221265591	0.0
17	0.0	0.0	0.0	9.945916768672879	0.0
18	0.0	0.0	0.0	10.725438834076169	0.0
19	9.57055942791024E-5	0.0	0.0	11.171714020199623	0.0
20	9.57055942791024E-5	0.0	0.0	11.596646858798838	0.0
21	9.57055942791024E-5	0.0	0.0	12.114892651820178	0.0
22	9.57055942791024E-5	0.0	0.0	12.613710209202859	0.0
23	9.57055942791024E-5	0.0	0.0	13.12851060083015	0.0
24	9.57055942791024E-5	0.0	0.0	13.567990689759789	0.0
25	9.57055942791024E-5	0.0	0.0	13.933968882283077	0.0
26	9.57055942791024E-5	0.0	0.0	14.288845225869988	0.0
27	1.914111885582048E-4	0.0	0.0	14.61797676459582	0.0
28	1.914111885582048E-4	0.0	0.0	14.972374580211337	0.0
29	1.914111885582048E-4	0.0	0.0	15.30485581473694	0.0
30	1.914111885582048E-4	0.0	0.0	15.690166537304606	0.0
31	2.871167828373072E-4	0.0	0.0	16.06724657876427	0.0
32	2.871167828373072E-4	0.0	0.0	16.42642967409374	0.0
33	2.871167828373072E-4	0.0	0.0	16.806476588976054	0.0
34	2.871167828373072E-4	0.0	0.0	17.152835134672127	0.0
35	2.871167828373072E-4	0.0	0.0	17.528479592217604	0.0
36	3.828223771164096E-4	0.0	0.0	17.887949804329914	0.0
37	4.7852797139551196E-4	0.0	0.0	18.243687498265338	0.0
38	5.742335656746144E-4	0.0	0.0	18.630529510341468	0.0
39	5.742335656746144E-4	0.0	0.0	18.973251243454932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGC	30	2.1653104E-6	45.000004	26
AACGAGT	30	2.1653104E-6	45.000004	29
CGGCCTA	20	7.032993E-4	45.0	45
GCTATCG	25	3.8905615E-5	45.0	1
TAAACCG	20	7.032993E-4	45.0	25
CGATTCG	25	3.8905615E-5	45.0	10
CGGATAG	20	7.032993E-4	45.0	1
TATTACG	25	3.8905615E-5	45.0	1
AATTACG	25	3.8905615E-5	45.0	1
GCTCGAT	20	7.032993E-4	45.0	22
TATCGAG	20	7.032993E-4	45.0	41
TAGTCCG	20	7.032993E-4	45.0	1
TGCGTAG	25	3.8905615E-5	45.0	1
CGTTTTT	28380	0.0	44.381603	1
CTCATGC	100	0.0	40.5	23
GCGTATG	95	0.0	40.26316	1
GTTTTTT	31560	0.0	40.18061	2
GTTTCGG	45	1.9279469E-8	40.0	2
TACTAGT	45	1.9279469E-8	40.0	40
ACCGTAG	45	1.9279469E-8	40.0	1
>>END_MODULE