##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1427771
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56607046928394	34.0	31.0	34.0	31.0	34.0
2	32.73497780806586	34.0	31.0	34.0	31.0	34.0
3	32.79428423745825	34.0	31.0	34.0	31.0	34.0
4	36.18550173662303	37.0	37.0	37.0	35.0	37.0
5	36.08077275697573	37.0	35.0	37.0	35.0	37.0
6	36.07154158474994	37.0	35.0	37.0	35.0	37.0
7	36.24210184966637	37.0	37.0	37.0	35.0	37.0
8	36.28398321579581	37.0	37.0	37.0	35.0	37.0
9	38.104826334195046	39.0	39.0	39.0	37.0	39.0
10	37.539614546030144	39.0	37.0	39.0	34.0	39.0
11	37.493579152399086	39.0	37.0	39.0	35.0	39.0
12	37.51673202495358	39.0	37.0	39.0	35.0	39.0
13	37.52740880715465	39.0	37.0	39.0	35.0	39.0
14	38.82156452260202	40.0	38.0	41.0	35.0	41.0
15	38.851072055672795	40.0	38.0	41.0	35.0	41.0
16	38.93419182768105	40.0	38.0	41.0	35.0	41.0
17	38.94818426764516	40.0	38.0	41.0	35.0	41.0
18	38.45988887573708	39.0	38.0	41.0	35.0	41.0
19	37.977959350624154	38.0	37.0	41.0	35.0	41.0
20	37.40280128956254	38.0	35.0	41.0	34.0	41.0
21	37.35246548641204	38.0	35.0	41.0	34.0	41.0
22	37.181503196240854	38.0	35.0	40.0	34.0	41.0
23	37.071701974616374	38.0	35.0	40.0	34.0	41.0
24	36.86544831068848	38.0	35.0	40.0	33.0	41.0
25	36.734818118591846	38.0	35.0	40.0	33.0	41.0
26	36.85946555855246	38.0	35.0	40.0	33.0	41.0
27	36.91165670124971	38.0	35.0	40.0	34.0	41.0
28	36.87690182809428	38.0	35.0	40.0	33.0	41.0
29	36.74886729034278	38.0	35.0	40.0	33.0	41.0
30	36.445370441058124	38.0	35.0	40.0	33.0	41.0
31	35.94198649503317	38.0	35.0	40.0	31.0	41.0
32	35.18393776032711	38.0	35.0	40.0	24.0	41.0
33	34.21979294998988	38.0	35.0	41.0	17.0	41.0
34	33.401095133603356	38.0	33.0	41.0	12.0	41.0
35	32.887298453323396	38.0	33.0	41.0	10.0	41.0
36	32.6743266252081	38.0	33.0	41.0	8.0	41.0
37	32.47535494137365	38.0	31.0	41.0	8.0	41.0
38	32.36996339048769	38.0	31.0	40.0	8.0	41.0
39	32.32541352920041	38.0	31.0	40.0	8.0	41.0
40	32.18607956037768	38.0	31.0	40.0	8.0	41.0
41	32.06533400664392	38.0	30.0	40.0	7.0	41.0
42	31.918352452879347	38.0	30.0	40.0	7.0	41.0
43	31.790492312842886	38.0	29.0	40.0	7.0	41.0
44	31.688676965703884	38.0	29.0	40.0	7.0	41.0
45	31.65912810947974	37.0	28.0	40.0	7.0	41.0
46	31.616173041755296	37.0	28.0	40.0	7.0	41.0
47	31.5940679562759	37.0	28.0	40.0	7.0	41.0
48	31.53265054409986	37.0	28.0	40.0	7.0	41.0
49	31.437292114771907	37.0	27.0	40.0	7.0	41.0
50	31.340366907578318	37.0	27.0	40.0	7.0	41.0
51	30.768848785974782	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	6.0
11	6.0
12	8.0
13	17.0
14	25.0
15	47.0
16	98.0
17	176.0
18	434.0
19	783.0
20	1357.0
21	2384.0
22	4097.0
23	7240.0
24	14950.0
25	33273.0
26	59635.0
27	71658.0
28	64408.0
29	49500.0
30	38070.0
31	32497.0
32	32160.0
33	38256.0
34	63854.0
35	90784.0
36	97341.0
37	128600.0
38	255070.0
39	340831.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.915050802964902	15.930635935314557	19.792739872150367	36.36157338957018
2	49.76708449744392	17.65836398133874	19.547252325477967	13.027299195739372
3	22.64179619840997	17.944124092729155	46.38488945356083	13.029190255300044
4	20.985087944775458	18.880478732233673	46.79069682743241	13.343736495558462
5	18.346989818395247	21.67763597943928	45.59876899026525	14.376605211900229
6	18.956751467847436	24.28141487675545	45.154510071993336	11.607323583403781
7	59.44307595545785	4.2242768623259614	32.33410680004006	3.9985403821761336
8	61.64447940180884	2.661701351267115	31.627130681320743	4.066688565603307
9	57.6851609957059	4.281428884604043	33.4281898147532	4.605220304936855
10	27.09370060044643	19.714506037732942	40.22858007341513	12.963213288405493
11	19.859767427689732	21.64387706431914	44.549511091064325	13.946844416926805
12	18.309378744910774	18.375355711805323	48.20773079156251	15.107534751721388
13	16.61926177237106	19.183398458156105	49.44378335181202	14.753556417660816
14	14.250184378307166	21.653542479851463	48.23938852939302	15.856884612448354
15	13.329798686203882	20.432058082143424	51.611708040014825	14.626435191637874
16	14.790817294930351	20.365730919033933	49.19367321510242	15.649778570933293
17	15.122453110477801	19.801774934495796	47.03709488426365	18.03867707076275
18	15.5350542909192	19.933868946770875	49.120202049208174	15.410874713101752
19	15.991780194442947	20.782604493297594	47.52526840788894	15.700346904370518
20	16.41691839937917	21.30537740295888	47.824966328633934	14.452737869028015
21	16.378396815735858	21.080201236752952	48.267194108859194	14.274207838651998
22	15.374034071290144	19.72984463194728	47.07043356392587	17.82568773283671
23	14.576707329116504	19.657213936968883	48.169069129433225	17.597009604481393
24	15.9447838623981	18.907794036998933	48.23847801923418	16.90894408136879
25	14.300612633258414	20.866861702611974	47.13395915731585	17.69856650681377
26	13.541737435485102	22.908995910408603	47.03268241195542	16.516584242150877
27	14.550652730725025	22.549974750852904	47.25141496780646	15.64795755061561
28	12.918037976678331	21.31343191590248	48.821204520893055	16.94732558652613
29	14.725820877437629	20.987189122065093	47.75758857687963	16.529401423617653
30	15.25195567076233	22.78250503757255	45.73492527863362	16.230614013031502
31	15.456960534987754	23.05698883084192	44.48444463432861	17.00160599984171
32	16.821044831419044	24.66235831936634	42.23674524836266	16.27985160085196
33	16.975131165992305	25.34783239048839	40.241327215638925	17.43570922788038
34	15.697965570108932	25.124617323086124	39.824663759104226	19.352753347700716
35	16.553775080177424	27.28840969595264	36.25938613405091	19.898429089819025
36	18.38362034247789	28.226795473503802	35.8162478436668	17.5733363403515
37	17.58440254074358	27.104276526137593	37.66605429021881	17.64526664290002
38	17.78051242110955	27.961556860308832	35.261817196175016	18.996113522406606
39	19.185009360744825	26.326840928972505	35.44714103312086	19.041008677161813
40	18.16670880694453	26.653573997510804	35.89553226672905	19.284184928815616
41	17.161435552339977	27.63587438041535	34.048177193681624	21.154512873563057
42	19.034845223778884	26.85962945038105	34.356700059043085	19.748825266796988
43	19.68922187101433	26.14326807310136	34.64771311365758	19.51979694222673
44	18.052824997846294	26.059220981515946	34.68539422638504	21.20255979425272
45	18.088194815555156	25.993244014621393	33.87216857605316	22.04639259377029
46	18.711263921175032	26.309331118225543	35.011356863250484	19.968048097348944
47	16.55776731702773	25.947648467436306	37.09376363576512	20.400820579770844
48	17.38668175778889	24.906305002693006	36.68403406428622	21.02297917523188
49	18.393565914982165	23.352834593222582	37.951954480095196	20.301645011700057
50	17.347179624743745	23.356896869315875	37.35830185653021	21.937621649410165
51	16.951808098077354	23.814253126026514	35.2423462866244	23.99159248927174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	428.0
1	662.0
2	896.0
3	51138.0
4	101380.0
5	69265.5
6	37151.0
7	36377.0
8	35603.0
9	35698.5
10	35794.0
11	35063.0
12	34332.0
13	32617.5
14	30903.0
15	29251.0
16	27599.0
17	25347.5
18	23096.0
19	20756.0
20	18416.0
21	17173.5
22	15931.0
23	15150.5
24	14370.0
25	14017.5
26	14395.0
27	15125.0
28	15802.0
29	16479.0
30	17871.5
31	19264.0
32	21486.5
33	23709.0
34	28020.5
35	32332.0
36	35177.5
37	38023.0
38	42209.0
39	46395.0
40	54112.5
41	61830.0
42	71159.5
43	80489.0
44	93495.5
45	106502.0
46	120104.5
47	133707.0
48	129543.5
49	125380.0
50	105573.5
51	85767.0
52	73805.5
53	61844.0
54	54313.0
55	46782.0
56	42255.5
57	37729.0
58	34024.0
59	30319.0
60	27844.0
61	25369.0
62	22427.5
63	19486.0
64	16645.0
65	13804.0
66	10564.0
67	7324.0
68	5795.5
69	4267.0
70	3478.5
71	2690.0
72	2201.5
73	1713.0
74	1210.0
75	721.0
76	735.0
77	439.0
78	143.0
79	147.5
80	152.0
81	124.0
82	96.0
83	61.0
84	26.0
85	17.5
86	9.0
87	5.0
88	1.0
89	1.5
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1427771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.0215333207896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12809215324926	26.6612251599316
2	10.429020053883791	7.304805466405484
3	4.073052408033191	4.279336218757701
4	2.053441950150394	2.8765874271799685
5	1.2100470426493874	2.118885141193422
6	0.7765491913619478	1.6317566028308805
7	0.5411808700793059	1.3267088711839425
8	0.43350358855081195	1.21455682968913
9	0.315568401966216	0.9946520374003345
>10	3.4366301512210913	29.173205838078296
>50	0.5264734838475043	11.642321781104878
>100	0.07032544900235593	4.243692381482048
>500	0.0036691536028989925	0.9002095816142838
>1k	0.00224226053510494	1.731100883787848
>5k	0.0	0.0
>10k+	2.038418668277218E-4	3.9009557793601046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54644	3.827224393827862	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4886	0.34221174123861603	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4841	0.33905997530416293	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	2983	0.2089270618327449	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2301	0.16116029811503385	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	1621	0.11353361288329851	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1615	0.11311337742537145	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.00392429878461E-5	0.0	0.0	0.2282578928973904	0.0
2	7.00392429878461E-5	0.0	0.0	1.2536324102394572	0.0
3	7.00392429878461E-5	0.0	0.0	1.7885921481806255	0.0
4	7.00392429878461E-5	0.0	0.0	2.353178485905653	0.0
5	7.00392429878461E-5	0.0	0.0	3.7339321221680506	0.0
6	1.400784859756922E-4	0.0	0.0	4.476208019353243	0.0
7	1.400784859756922E-4	0.0	0.0	5.260437423088156	0.0
8	1.400784859756922E-4	0.0	0.0	6.4130032056961515	0.0
9	1.400784859756922E-4	0.0	0.0	6.806273555072908	0.0
10	1.400784859756922E-4	0.0	0.0	7.75957769138048	0.0
11	1.400784859756922E-4	0.0	0.0	9.203716842546878	0.0
12	1.400784859756922E-4	0.0	0.0	10.288274520213676	0.0
13	1.400784859756922E-4	0.0	0.0	10.708930213598679	0.0
14	1.400784859756922E-4	0.0	0.0	10.863786979844807	0.0
15	1.400784859756922E-4	0.0	0.0	11.161453762543154	0.0
16	1.400784859756922E-4	0.0	0.0	11.888881340214922	0.0
17	1.400784859756922E-4	0.0	0.0	12.777119019786786	0.0
18	1.400784859756922E-4	0.0	0.0	13.753325988551385	0.0
19	1.400784859756922E-4	0.0	0.0	14.317211933846535	0.0
20	1.400784859756922E-4	0.0	0.0	14.884950037506014	0.0
21	1.400784859756922E-4	0.0	0.0	15.635840761578713	0.0
22	1.400784859756922E-4	0.0	0.0	16.390093369314826	0.0
23	1.400784859756922E-4	0.0	0.0	17.14609695812564	0.0
24	1.400784859756922E-4	0.0	0.0	17.751936409970508	0.0
25	1.400784859756922E-4	0.0	0.0	18.293059601294605	0.0
26	1.400784859756922E-4	0.0	0.0	18.77612026018178	0.0
27	1.400784859756922E-4	0.0	0.0	19.249445464293643	0.0
28	2.1011772896353828E-4	0.0	0.0	19.722420472190567	0.0
29	2.1011772896353828E-4	0.0	0.0	20.227053217917998	0.0
30	2.1011772896353828E-4	0.0	0.0	20.791779634129004	0.0
31	2.1011772896353828E-4	0.0	0.0	21.35671616806897	0.0
32	2.1011772896353828E-4	0.0	0.0	21.854485067983592	0.0
33	2.1011772896353828E-4	0.0	0.0	22.34034729659028	0.0
34	2.1011772896353828E-4	0.0	0.0	22.81381257918812	0.0
35	2.1011772896353828E-4	0.0	0.0	23.315433637467073	0.0
36	2.1011772896353828E-4	0.0	0.0	23.798004021653334	0.0
37	2.1011772896353828E-4	0.0	0.0	24.270768911821293	0.0
38	2.1011772896353828E-4	0.0	0.0	24.752919060549626	0.0
39	2.1011772896353828E-4	0.0	0.0	25.232057521829482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	25	3.8912818E-5	45.0	36
TCGTTGA	40	6.8175723E-9	45.0	24
GTCGATT	25	3.8912818E-5	45.0	14
CGGCCTA	20	7.0338626E-4	45.0	11
TAGGTCG	20	7.0338626E-4	45.0	1
TTCGTCG	20	7.0338626E-4	45.0	10
CCGCTAT	20	7.0338626E-4	45.0	41
TAGTTCG	20	7.0338626E-4	45.0	1
ATTCCGC	20	7.0338626E-4	45.0	42
GTTCGTC	25	3.8912818E-5	45.0	9
TCGTCGA	20	7.0338626E-4	45.0	11
ACCCGCG	20	7.0338626E-4	45.0	32
TATCGCG	25	3.8912818E-5	45.0	1
TAGTCCG	35	1.2122109E-7	45.0	1
TAATCGT	40	6.8175723E-9	45.0	21
CGTTTTT	38770	0.0	44.622776	1
CTTGACG	50	1.0822987E-9	40.5	1
TCGAGCG	50	1.0822987E-9	40.5	1
CTAGCGG	100	0.0	40.5	2
GTTTTTT	43725	0.0	39.622643	2
>>END_MODULE