##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934904.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1389803
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66534897391932	34.0	31.0	34.0	31.0	34.0
2	32.82556664505689	34.0	31.0	34.0	31.0	34.0
3	32.870031939778514	34.0	31.0	34.0	31.0	34.0
4	36.250765756010026	37.0	37.0	37.0	35.0	37.0
5	36.14393766598575	37.0	37.0	37.0	35.0	37.0
6	36.13721009380466	37.0	36.0	37.0	35.0	37.0
7	36.29460002604686	37.0	37.0	37.0	35.0	37.0
8	36.31819761505767	37.0	37.0	37.0	35.0	37.0
9	38.112054010532425	39.0	39.0	39.0	37.0	39.0
10	37.7153733298892	39.0	38.0	39.0	35.0	39.0
11	37.553932463809616	39.0	37.0	39.0	35.0	39.0
12	37.60695508644031	39.0	37.0	39.0	35.0	39.0
13	37.61853370585615	39.0	37.0	39.0	35.0	39.0
14	38.95650678549406	40.0	38.0	41.0	35.0	41.0
15	38.999643834413945	40.0	38.0	41.0	35.0	41.0
16	39.073029774723466	41.0	38.0	41.0	35.0	41.0
17	39.08328806312837	41.0	38.0	41.0	35.0	41.0
18	38.56310210871613	39.0	38.0	41.0	35.0	41.0
19	38.07591219762801	39.0	37.0	41.0	35.0	41.0
20	37.51281728417624	39.0	35.0	41.0	35.0	41.0
21	37.46679853187826	39.0	35.0	41.0	35.0	41.0
22	37.27343515591778	38.0	35.0	41.0	34.0	41.0
23	37.171864645564874	38.0	35.0	40.0	34.0	41.0
24	36.99160096790696	38.0	35.0	40.0	34.0	41.0
25	36.8606629860491	38.0	35.0	40.0	33.0	41.0
26	36.98617142141728	38.0	35.0	40.0	34.0	41.0
27	37.0281514718273	38.0	35.0	40.0	34.0	41.0
28	36.9729076710872	38.0	35.0	40.0	34.0	41.0
29	36.84223015779934	38.0	35.0	41.0	33.0	41.0
30	36.52054499810405	38.0	35.0	41.0	33.0	41.0
31	35.99314507163965	38.0	35.0	41.0	31.0	41.0
32	35.24641765775437	38.0	35.0	41.0	23.0	41.0
33	34.272882559614565	38.0	35.0	41.0	16.0	41.0
34	33.47993564555552	38.0	34.0	41.0	12.0	41.0
35	32.980437515244965	38.0	33.0	41.0	10.0	41.0
36	32.72987754379577	38.0	33.0	41.0	8.0	41.0
37	32.51190276607548	38.0	31.0	41.0	8.0	41.0
38	32.36527263216441	38.0	31.0	41.0	8.0	41.0
39	32.26501022087303	38.0	31.0	41.0	7.0	41.0
40	32.14318144370101	38.0	30.0	41.0	7.0	41.0
41	32.031194349127176	38.0	30.0	41.0	7.0	41.0
42	31.9252476789876	38.0	29.0	41.0	7.0	41.0
43	31.833369189734086	38.0	29.0	41.0	7.0	41.0
44	31.727974396371284	38.0	28.0	41.0	7.0	41.0
45	31.68899405167495	38.0	27.0	40.0	7.0	41.0
46	31.643991990231708	37.0	27.0	40.0	7.0	41.0
47	31.64644917301229	37.0	27.0	40.0	7.0	41.0
48	31.61019295540447	37.0	27.0	40.0	7.0	41.0
49	31.502258233720895	37.0	27.0	40.0	7.0	41.0
50	31.430647365130167	37.0	27.0	40.0	7.0	41.0
51	30.918154587376772	36.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	8.0
10	9.0
11	13.0
12	25.0
13	23.0
14	25.0
15	43.0
16	91.0
17	188.0
18	381.0
19	691.0
20	1348.0
21	2214.0
22	3674.0
23	6803.0
24	13587.0
25	30101.0
26	55582.0
27	70701.0
28	65395.0
29	51378.0
30	38161.0
31	32557.0
32	30791.0
33	35208.0
34	59479.0
35	81103.0
36	87454.0
37	118813.0
38	241571.0
39	362118.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.553905121805034	16.41721884324613	18.628539440481852	37.400336594466985
2	50.7358956629105	18.461681259862008	18.29957195372294	12.502851123504556
3	23.16040474801105	17.871309818729706	46.7041012287353	12.26418420452395
4	21.613350956934184	19.060902876163023	46.12142872047333	13.204317446429458
5	18.581554364179674	21.742937668144336	45.61027713999754	14.065230827678455
6	20.07816935205925	24.309344561783217	44.28174352767982	11.330742558477711
7	59.77955149039108	4.341118849218199	32.24795168811695	3.631377972273768
8	60.5752038238513	3.5918040182673376	32.08001421784238	3.752977940038984
9	55.84100768238376	6.239589351872172	33.62044836570363	4.298954600040437
10	26.75235267156568	26.154642060781274	38.347089479588114	8.745915788064927
11	18.632640741169794	18.868717364979066	48.63077716769931	13.867864726151835
12	18.968371776431624	16.617535003162317	50.1675417307345	14.246551489671559
13	16.784033420563922	18.673293984830945	50.2934588571186	14.249213737486535
14	13.618764673842264	21.300932578214322	48.807564813142584	16.272737934800833
15	13.283177543867728	20.82784394622835	51.7705027259259	14.118475783978019
16	14.96773283695603	19.964556127738966	50.06450554503048	15.00320549027452
17	15.223092769262983	19.930522527293437	47.58911874560639	17.25726595783719
18	14.898010725261063	20.58025489943539	48.97341565675135	15.548318718552197
19	15.9536279602217	20.826620751286335	47.81584152574142	15.403909762750548
20	17.662647152150342	19.984630915316774	48.16905705340972	14.183664879123157
21	17.251509746345345	19.825471667567275	48.96643625031749	13.956582335769891
22	15.898800045761883	19.197253135876093	47.50961107437529	17.394335743986737
23	14.675173387883031	19.733588141628704	48.64452012263609	16.946718347852176
24	15.646246266557204	18.968587634362567	49.088899649806486	16.296266449273748
25	14.680497883512988	20.547804257150116	47.67064109085964	17.10105676847726
26	13.841242248002056	22.18479885278705	48.31159524047653	15.662363658734368
27	15.224963537997832	21.35137138141161	48.58436771254631	14.83929736804425
28	14.255977285989452	20.351589397921863	49.388726315887936	16.003707000200748
29	15.344189068522663	19.40713899739747	48.9215378006811	16.32713413339876
30	15.754750853178473	19.574500846522852	48.76827866971074	15.902469630587932
31	16.698121964048145	21.6886853748337	45.34779389596943	16.26539876514873
32	16.80936075112804	22.396339625112336	44.207920115296915	16.58637950846271
33	16.796840991133273	23.634572669651742	43.10402265644843	16.46456368276655
34	16.62645713097468	24.309128703852274	41.23936989630905	17.825044268864005
35	16.438157062547713	24.096868405090504	40.031860630607355	19.43311390175442
36	17.476361757745522	26.101900772987253	39.207571144975226	17.214166324292005
37	18.389944474144897	26.987493911007533	38.2699562455974	16.352605369250174
38	18.013488242578266	26.823010167628077	36.06863706582875	19.094864523964908
39	18.263307821324318	26.581249284970603	37.24103344143019	17.91440945227489
40	17.913258209976522	25.9242496958202	38.04827015051774	18.114221943685543
41	16.00356309491345	27.15197765438699	36.03395589159039	20.81050335910917
42	17.493774297508352	26.912159493108017	35.76622010457597	19.82784610480766
43	19.096519434768812	26.104563020802228	36.19606519772946	18.602852346699496
44	18.367711107257648	26.129170824929865	36.055325826753865	19.447792241058625
45	17.945780804905443	24.916984637391053	36.43199791625144	20.705236641452064
46	18.022626228321567	25.484834901061515	37.30859697381571	19.1839418968012
47	16.312455794094557	25.07564021663502	39.14972121948219	19.462182769788235
48	16.82785258054559	23.961597435032157	38.66080300589364	20.549746978528614
49	17.56565498851276	23.30171974013583	39.683897645925356	19.44872762542605
50	16.85598606421198	23.522038734986182	39.14238204983008	20.479593150971755
51	15.972551505501139	23.329349555296687	37.31068360048151	23.38741533872067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	441.0
1	832.5
2	1224.0
3	54682.5
4	108141.0
5	75643.5
6	43146.0
7	41160.5
8	39175.0
9	37859.5
10	36544.0
11	35110.0
12	33676.0
13	31400.5
14	29125.0
15	26501.0
16	23877.0
17	21520.5
18	19164.0
19	18047.0
20	16930.0
21	15903.5
22	14877.0
23	14465.5
24	14054.0
25	14604.0
26	16402.5
27	17651.0
28	18739.0
29	19827.0
30	21700.5
31	23574.0
32	26343.5
33	29113.0
34	33033.0
35	36953.0
36	37946.5
37	38940.0
38	43034.5
39	47129.0
40	53459.5
41	59790.0
42	67131.0
43	74472.0
44	85488.0
45	96504.0
46	104552.5
47	112601.0
48	110920.0
49	109239.0
50	91595.5
51	73952.0
52	64074.0
53	54196.0
54	49035.5
55	43875.0
56	39982.5
57	36090.0
58	33299.5
59	30509.0
60	29408.5
61	28308.0
62	25565.0
63	22822.0
64	18868.5
65	14915.0
66	11584.0
67	8253.0
68	6941.5
69	5630.0
70	4841.0
71	4052.0
72	3174.0
73	2296.0
74	1937.0
75	1359.0
76	1140.0
77	824.5
78	509.0
79	330.5
80	152.0
81	123.0
82	94.0
83	66.0
84	38.0
85	42.5
86	47.0
87	32.5
88	18.0
89	12.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1389803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.61374058935133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26078265784655	24.8714938274391
2	10.34212538784684	6.745907890835627
3	3.934406811679482	3.849471693872745
4	2.011722216234112	2.6243914599237708
5	1.1624499591250892	1.895592070750386
6	0.7568803129736781	1.4810818910706351
7	0.4981814684549243	1.137329282502771
8	0.38011023629809265	0.9917453313586424
9	0.2904677972332234	0.8525917249672185
>10	3.540914735143804	29.0815312042843
>50	0.7315622767446206	15.195832823880265
>100	0.08432475791928633	4.980330640671974
>500	0.0033729902779683647	0.7589668893388862
>1k	0.0024735262038434675	1.6292596785730695
>5k	0.0	0.0
>10k+	2.248660185312243E-4	3.904473590530708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53240	3.830758747822533	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	4777	0.34371777870676634	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	4132	0.29730832355376985	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2392	0.17211072360615137	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	1670	0.1201609148922545	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1637	0.11778647765186864	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1501	0.10800091811573294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.439052872961132E-4	0.0	0.0	0.18117675670580652	0.0
2	1.439052872961132E-4	0.0	0.0	1.0789298915026087	0.0
3	1.439052872961132E-4	0.0	0.0	1.5213666972945086	0.0
4	1.439052872961132E-4	0.0	0.0	1.9635876451554646	0.0
5	1.439052872961132E-4	0.0	0.0	3.104684620769994	0.0
6	1.439052872961132E-4	0.0	0.0	3.6579284977799014	0.0
7	1.439052872961132E-4	0.0	0.0	4.257294019368213	0.0
8	1.439052872961132E-4	0.0	0.0	5.1886490387486575	0.0
9	1.439052872961132E-4	0.0	0.0	5.511428598153839	0.0
10	1.439052872961132E-4	0.0	0.0	6.202317882462478	0.0
11	1.439052872961132E-4	0.0	0.0	7.374642305420265	0.0
12	1.439052872961132E-4	0.0	0.0	8.324273296287316	0.0
13	1.439052872961132E-4	0.0	0.0	8.684252372458543	0.0
14	1.439052872961132E-4	0.0	0.0	8.829524759983968	0.0
15	1.439052872961132E-4	0.0	0.0	9.096756878492851	0.0
16	1.439052872961132E-4	0.0	0.0	9.688135656636229	0.0
17	1.439052872961132E-4	0.0	0.0	10.44147983563138	0.0
18	1.439052872961132E-4	0.0	0.0	11.291600320333169	0.0
19	1.439052872961132E-4	0.0	0.0	11.798218884259136	0.0
20	1.439052872961132E-4	0.0	0.0	12.280085738770172	0.0
21	1.439052872961132E-4	0.0	0.0	12.874342622659471	0.0
22	1.439052872961132E-4	0.0	0.0	13.526953100547344	0.0
23	1.439052872961132E-4	0.0	0.0	14.152941100285435	0.0
24	1.439052872961132E-4	0.0	0.0	14.646752093642048	0.0
25	1.439052872961132E-4	0.0	0.0	15.070121448867214	0.0
26	1.439052872961132E-4	0.0	0.0	15.481114949384914	0.0
27	1.439052872961132E-4	0.0	0.0	15.883114369446606	0.0
28	1.439052872961132E-4	0.0	0.0	16.295115206975378	0.0
29	1.439052872961132E-4	0.0	0.0	16.75086325184217	0.0
30	1.439052872961132E-4	0.0	0.0	17.245897440140798	0.0
31	1.439052872961132E-4	0.0	0.0	17.721000746148913	0.0
32	1.439052872961132E-4	0.0	0.0	18.15055802872781	0.0
33	1.439052872961132E-4	0.0	0.0	18.54133283638041	0.0
34	1.439052872961132E-4	0.0	0.0	18.943835924947635	0.0
35	2.1585793094416978E-4	0.0	0.0	19.373033444308295	0.0
36	2.1585793094416978E-4	0.0	0.0	19.813167765503458	0.0
37	2.1585793094416978E-4	0.0	0.0	20.234522446706475	0.0
38	2.1585793094416978E-4	0.0	0.0	20.6464513315916	0.0
39	2.878105745922264E-4	0.0	0.0	21.053559389352305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.033798E-4	45.0	1
GGCGTAT	20	7.033798E-4	45.0	37
TAGCGCG	40	6.8175723E-9	45.0	1
ATCGTCA	20	7.033798E-4	45.0	10
CGCCTAC	20	7.033798E-4	45.0	19
ACGATGC	20	7.033798E-4	45.0	11
TGATTCG	45	3.8562575E-10	45.0	1
ATGTTCG	20	7.033798E-4	45.0	40
TCGGTAA	20	7.033798E-4	45.0	37
ACGCGAG	25	3.8912294E-5	44.999996	1
GATCGTC	25	3.8912294E-5	44.999996	9
TCGATCA	25	3.8912294E-5	44.999996	17
AACGCGT	25	3.8912294E-5	44.999996	43
TAGGACG	30	2.1658307E-6	44.999996	1
TATCGTG	30	2.1658307E-6	44.999996	1
CGGAGTA	25	3.8912294E-5	44.999996	19
CGTTTTT	37750	0.0	44.46954	1
TCGTTGA	70	0.0	41.785717	24
CTAACGG	60	3.6379788E-12	41.249996	2
TACGGGT	55	6.184564E-11	40.909092	4
>>END_MODULE