##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934903.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441627
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57574601474584	34.0	31.0	34.0	31.0	34.0
2	32.73061062258129	34.0	31.0	34.0	31.0	34.0
3	32.78574901829669	34.0	31.0	34.0	31.0	34.0
4	36.19388371610687	37.0	37.0	37.0	35.0	37.0
5	36.07787867458087	37.0	35.0	37.0	35.0	37.0
6	36.05835351307932	37.0	35.0	37.0	35.0	37.0
7	36.20107350930581	37.0	37.0	37.0	35.0	37.0
8	36.18334839733163	37.0	37.0	37.0	35.0	37.0
9	37.95443412200243	39.0	39.0	39.0	35.0	39.0
10	37.60015316028348	39.0	38.0	39.0	35.0	39.0
11	37.514258542604985	39.0	37.0	39.0	35.0	39.0
12	37.468781453177556	39.0	37.0	39.0	35.0	39.0
13	37.42476174488963	39.0	37.0	39.0	35.0	39.0
14	38.726846819600354	40.0	38.0	41.0	35.0	41.0
15	38.76148546052481	40.0	38.0	41.0	35.0	41.0
16	38.856073034148224	40.0	38.0	41.0	35.0	41.0
17	38.848666125148874	40.0	38.0	41.0	35.0	41.0
18	38.37053481933954	39.0	38.0	41.0	35.0	41.0
19	37.87306494675807	38.0	37.0	41.0	35.0	41.0
20	37.30671317892909	38.0	35.0	41.0	34.0	41.0
21	37.26324493090099	38.0	35.0	41.0	34.0	41.0
22	37.084031445027044	38.0	35.0	41.0	34.0	41.0
23	36.948116260308666	38.0	35.0	40.0	33.0	41.0
24	36.73636106981903	38.0	35.0	40.0	33.0	41.0
25	36.578040644355305	37.0	35.0	40.0	33.0	41.0
26	36.6875724441898	38.0	35.0	40.0	33.0	41.0
27	36.72666577415656	38.0	35.0	40.0	33.0	41.0
28	36.65653806428431	38.0	35.0	40.0	33.0	41.0
29	36.49727842222711	38.0	35.0	40.0	33.0	41.0
30	36.211207892194025	38.0	35.0	40.0	32.0	41.0
31	35.720774513795874	38.0	35.0	40.0	30.0	41.0
32	34.991002527005946	38.0	35.0	40.0	23.0	41.0
33	34.049357427406676	38.0	34.0	40.0	16.0	41.0
34	33.28106923635587	38.0	33.0	40.0	12.0	41.0
35	32.82653557404238	38.0	33.0	41.0	10.0	41.0
36	32.60638986367486	38.0	32.0	40.0	9.0	41.0
37	32.39266675776744	38.0	31.0	40.0	8.0	41.0
38	32.243271664584526	37.0	31.0	40.0	8.0	41.0
39	32.16781594684339	37.0	31.0	40.0	8.0	41.0
40	32.02503144017142	37.0	30.0	40.0	8.0	41.0
41	31.909140159000906	37.0	30.0	40.0	8.0	41.0
42	31.77337411133393	37.0	29.0	40.0	7.0	41.0
43	31.631284652687555	37.0	29.0	40.0	7.0	41.0
44	31.51471635866975	36.0	28.0	40.0	7.0	41.0
45	31.47343522284197	36.0	27.0	40.0	7.0	41.0
46	31.423153839377314	36.0	27.0	40.0	7.0	41.0
47	31.399841984091584	36.0	27.0	40.0	7.0	41.0
48	31.360684837340035	36.0	27.0	40.0	7.0	41.0
49	31.23736445002764	36.0	27.0	40.0	7.0	41.0
50	31.14656634483122	35.0	27.0	40.0	7.0	41.0
51	30.613812726870403	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	14.0
10	14.0
11	14.0
12	19.0
13	26.0
14	42.0
15	70.0
16	146.0
17	289.0
18	546.0
19	964.0
20	1673.0
21	2804.0
22	4581.0
23	7831.0
24	14378.0
25	30644.0
26	56035.0
27	72687.0
28	68747.0
29	53564.0
30	40719.0
31	35336.0
32	35563.0
33	42750.0
34	73152.0
35	97029.0
36	103423.0
37	132378.0
38	240696.0
39	325275.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.845704193941987	15.412100356056039	17.51500214687988	36.22719330312209
2	50.327650633624366	18.855709555939228	17.657271957309344	13.159367853127058
3	23.456969105045896	18.529272828547192	45.147392494729914	12.866365571677003
4	21.216653128721923	19.27412569270692	45.31928161722831	14.189939561342843
5	19.4546855740077	21.80897000403017	43.85101000466833	14.885334417293794
6	19.68498092779894	25.014653582376024	43.52589123261426	11.774474257210777
7	58.36953664158621	4.688938262116345	32.908581762134034	4.032943334163415
8	58.30072549973051	5.294157226522533	31.97442889180072	4.430688381946232
9	53.56288415796874	6.550931690374834	33.76074393723203	6.125440214424397
10	29.697071433872978	20.100275591397775	38.768696757205575	11.433956217523674
11	24.290679905412425	18.507977444928542	42.85109809957777	14.350244550081262
12	23.034876566545993	15.934634964522724	45.657649308732424	15.37283916019886
13	18.86500460937538	17.996818872010582	46.780616622746386	16.357559895867656
14	14.573672662901012	21.321603993265942	46.33584137921945	17.76888196461359
15	14.033588438618311	19.245963068116787	50.96567974933877	15.754768743926133
16	15.710513190998782	18.39844841973687	48.9154961720334	16.97554221723095
17	15.632337629636513	18.856541948784255	46.4208841815532	19.090236240026027
18	16.51529834000057	19.391423717785532	46.80101024744958	17.29226769476432
19	18.252918403997707	20.471245335998837	44.77128966091784	16.504546599085618
20	20.38252613193288	19.436164833205815	45.428325079927056	14.752983954934251
21	18.504162311055495	19.951069173926403	45.46717007936172	16.07759843565638
22	17.19078513374125	19.62442434832311	44.87887643613778	18.30591408179786
23	17.333332408452396	20.014955324782346	45.62816872880433	17.02354353796093
24	18.054184612247134	18.108082048962736	45.9041069569313	17.93362638185883
25	16.534374009365806	20.49885303202562	44.401707237725155	18.56506572088342
26	16.023909097152035	21.514025472608377	45.04771345153774	17.41435197870184
27	17.610033663353974	21.117529014093105	44.82130259768997	16.45113472486295
28	15.448725641237296	21.556269409493577	45.22438883289506	17.770616116374068
29	16.391826734654664	20.384953944397548	44.707680974343575	18.515538346604217
30	17.357471800958223	20.694673448818595	43.58339570499165	18.364459045231534
31	17.082504697817118	21.721430023161332	41.25803692633392	19.938028352687624
32	18.317567581628257	22.722521151449023	40.35426639484416	18.60564487207856
33	18.293220090911174	24.24496766500627	38.61449598266403	18.847316261418523
34	17.170738339390148	24.624330704128045	37.447619946074816	20.757311010406994
35	17.567650994327934	25.23773486484368	35.54241145594526	21.65220268488312
36	17.99626394344723	27.93704612913049	34.56920548796602	19.497484439456255
37	18.653646192808544	26.516845203370913	35.37516986016494	19.454338743655605
38	17.61627660969169	26.052092531563297	34.312759125626805	22.018871733118207
39	18.860010252305205	25.49640094143631	34.907920009822234	20.73566879643625
40	18.885745064430676	25.61120178797983	36.38652716687465	19.116525980714847
41	16.592641508517804	26.658074522744098	35.18704907718848	21.56223489154962
42	18.37278297368182	25.98994053246783	34.906463322343434	20.730813171506917
43	19.683316142108882	26.18853559207756	33.97494636268605	20.153201903127506
44	18.750203762831855	26.288769563833085	32.92696377079508	22.03406290253998
45	17.963869988561534	25.381530728822366	33.13957077662946	23.51502850598664
46	18.212755449225078	26.313463884902266	33.8287226862427	21.645057979629957
47	17.192935481924245	24.949033279759604	36.752571920475965	21.10545931784019
48	17.298233176820357	23.762873475593896	36.3783419705652	22.560551377020545
49	17.044769555509156	23.69489472658323	37.28349982346335	21.976835894444264
50	16.493031831396053	22.95649290697247	36.925570900101064	23.62490436153041
51	15.867557974427504	23.10500566373965	35.56842373235241	25.459012629480444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	366.0
1	854.0
2	1342.0
3	56792.0
4	112242.0
5	78148.0
6	44054.0
7	42400.0
8	40746.0
9	39289.0
10	37832.0
11	35383.5
12	32935.0
13	30283.0
14	27631.0
15	25334.5
16	23038.0
17	21127.5
18	19217.0
19	17754.0
20	16291.0
21	14952.0
22	13613.0
23	12699.5
24	11786.0
25	11736.0
26	12444.5
27	13203.0
28	14604.5
29	16006.0
30	17064.5
31	18123.0
32	20069.5
33	22016.0
34	24372.5
35	26729.0
36	28444.5
37	30160.0
38	32652.0
39	35144.0
40	39661.0
41	44178.0
42	49520.0
43	54862.0
44	60696.5
45	66531.0
46	79026.5
47	91522.0
48	97064.5
49	102607.0
50	101977.5
51	101348.0
52	88667.5
53	75987.0
54	68843.0
55	61699.0
56	57439.0
57	53179.0
58	53656.0
59	54133.0
60	53611.0
61	53089.0
62	48643.0
63	44197.0
64	36162.0
65	28127.0
66	23568.0
67	19009.0
68	15300.5
69	11592.0
70	10358.0
71	9124.0
72	7799.5
73	6475.0
74	5408.0
75	3555.5
76	2770.0
77	2033.5
78	1297.0
79	1028.5
80	760.0
81	573.5
82	387.0
83	268.0
84	149.0
85	97.0
86	45.0
87	37.5
88	30.0
89	24.0
90	18.0
91	12.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1441627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.345874756011234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.43299866751579	25.046380768818505
2	9.649448975596416	6.242397360583251
3	3.689256725488793	3.5799670795629783
4	1.853199721229511	2.3977346432305877
5	1.0606078980141975	1.715314511720178
6	0.7128594682577674	1.3834837847341503
7	0.5088987172782505	1.1522541920803944
8	0.38053754369034154	0.9847055782534352
9	0.2929878780930027	0.8529254288843177
>10	3.5496776142521362	28.7588205024632
>50	0.7688970434818594	15.89788888222535
>100	0.09298974471662846	5.334599454175886
>500	0.004147429866309026	0.8753787149955919
>1k	0.003274286736559757	1.8952182180925696
>5k	0.0	0.0
>10k+	2.1828578243731711E-4	3.8829308801796727
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54994	3.814717676625091	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	4350	0.30174240632285604	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	3302	0.2290467645236944	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	2088	0.1448363550349709	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	2043	0.14171488186611378	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1919	0.13311348913415189	TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	1890	0.13110187309199953	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1559	0.10814170378329485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.080982112571421E-4	0.0	0.0	0.14199234614778997	0.0
2	2.080982112571421E-4	0.0	0.0	0.880186067547292	0.0
3	2.080982112571421E-4	0.0	0.0	1.2528205978384146	0.0
4	2.080982112571421E-4	0.0	0.0	1.6759536273946034	0.0
5	2.080982112571421E-4	0.0	0.0	2.8225054053510372	0.0
6	2.080982112571421E-4	0.0	0.0	3.3924170399139304	0.0
7	2.080982112571421E-4	0.0	0.0	4.0167810397557755	0.0
8	2.080982112571421E-4	0.0	0.0	4.9265864193720015	0.0
9	2.080982112571421E-4	0.0	0.0	5.291382583705771	0.0
10	2.080982112571421E-4	0.0	0.0	6.196956633026435	0.0
11	2.080982112571421E-4	0.0	0.0	7.287876822506792	0.0
12	2.080982112571421E-4	0.0	0.0	8.33585941439776	0.0
13	2.080982112571421E-4	0.0	0.0	8.687545391422331	0.0
14	2.080982112571421E-4	0.0	0.0	8.84126060347094	0.0
15	2.080982112571421E-4	0.0	0.0	9.15132693824408	0.0
16	2.080982112571421E-4	0.0	0.0	9.705769939103527	0.0
17	2.080982112571421E-4	0.0	0.0	10.36377648309861	0.0
18	4.161964225142842E-4	0.0	0.0	11.075680463809293	0.0
19	4.161964225142842E-4	0.0	0.0	11.550075019405158	0.0
20	4.161964225142842E-4	0.0	0.0	11.983474227383367	0.0
21	4.161964225142842E-4	0.0	0.0	12.521477469553497	0.0
22	4.161964225142842E-4	0.0	0.0	13.099435568285001	0.0
23	4.8556249293333156E-4	0.0	0.0	13.643681756792846	0.0
24	4.8556249293333156E-4	0.0	0.0	14.074930616588063	0.0
25	4.8556249293333156E-4	0.0	0.0	14.469484825131605	0.0
26	4.8556249293333156E-4	0.0	0.0	14.82228065928288	0.0
27	4.8556249293333156E-4	0.0	0.0	15.186660627194135	0.0
28	4.8556249293333156E-4	0.0	0.0	15.531063166824707	0.0
29	4.8556249293333156E-4	0.0	0.0	15.902241009637029	0.0
30	4.8556249293333156E-4	0.0	0.0	16.313304342940302	0.0
31	4.8556249293333156E-4	0.0	0.0	16.720830006652207	0.0
32	5.54928563352379E-4	0.0	0.0	17.118575054435023	0.0
33	5.54928563352379E-4	0.0	0.0	17.470052933248336	0.0
34	5.54928563352379E-4	0.0	0.0	17.813623080033878	0.0
35	5.54928563352379E-4	0.0	0.0	18.19735618159205	0.0
36	6.242946337714264E-4	0.0	0.0	18.612373380909208	0.0
37	6.242946337714264E-4	0.0	0.0	19.006303294818977	0.0
38	6.242946337714264E-4	0.0	0.0	19.3862212625041	0.0
39	6.936607041904736E-4	0.0	0.0	19.755040658922177	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	230	0.0	45.000004	12
CGAATAT	230	0.0	45.000004	14
AACGTCG	20	7.033885E-4	45.0	2
CTATGCG	35	1.2122109E-7	45.0	1
TCCGCAC	25	3.8913015E-5	45.0	33
AGATTCG	20	7.033885E-4	45.0	1
CGTACAT	25	3.8913015E-5	45.0	35
AATACGG	35	1.2122109E-7	45.0	2
TCGCGAT	20	7.033885E-4	45.0	33
CGTTTTT	38095	0.0	44.486153	1
GCTACGA	255	0.0	42.35294	10
CGGTCTA	70	0.0	41.785713	31
GTTTTTT	41750	0.0	40.78563	2
ATAGGGT	145	0.0	40.34483	4
TATACGG	45	1.9288564E-8	40.0	2
TCGACAG	45	1.9288564E-8	40.0	1
CCTATGC	220	0.0	39.886364	35
TCACGAC	80	0.0	39.375	25
TCGATAG	40	3.4590448E-7	39.375	1
CGTATAG	75	0.0	39.0	1
>>END_MODULE