##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	534972
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.471015305473934	34.0	31.0	34.0	31.0	34.0
2	32.653361297413696	34.0	31.0	34.0	31.0	34.0
3	32.71786560791967	34.0	31.0	34.0	31.0	34.0
4	36.131251729062456	37.0	37.0	37.0	35.0	37.0
5	36.01112020816043	37.0	35.0	37.0	35.0	37.0
6	35.996033437264	37.0	35.0	37.0	35.0	37.0
7	36.16193931645021	37.0	36.0	37.0	35.0	37.0
8	36.17185385403348	37.0	37.0	37.0	35.0	37.0
9	37.9736752577705	39.0	39.0	39.0	35.0	39.0
10	37.56625019627195	39.0	37.0	39.0	35.0	39.0
11	37.454762118391244	39.0	37.0	39.0	35.0	39.0
12	37.38220131147051	39.0	37.0	39.0	35.0	39.0
13	37.27387788519773	39.0	37.0	39.0	34.0	39.0
14	38.48451881593803	40.0	38.0	41.0	34.0	41.0
15	38.56435476996927	40.0	38.0	41.0	34.0	41.0
16	38.66389082045416	40.0	38.0	41.0	35.0	41.0
17	38.672738012456726	40.0	38.0	41.0	35.0	41.0
18	38.21023343277779	39.0	38.0	40.0	35.0	41.0
19	37.72763247422295	38.0	37.0	40.0	34.0	41.0
20	37.171893108424364	38.0	35.0	40.0	34.0	41.0
21	37.12813941664237	38.0	35.0	40.0	34.0	41.0
22	36.89707124858871	38.0	35.0	40.0	34.0	41.0
23	36.78795525747142	37.0	35.0	40.0	33.0	41.0
24	36.60021832918358	37.0	35.0	40.0	33.0	41.0
25	36.4520647809605	37.0	35.0	40.0	33.0	41.0
26	36.59827430220647	37.0	35.0	40.0	33.0	41.0
27	36.67178843004868	37.0	35.0	40.0	33.0	41.0
28	36.6423551139125	37.0	35.0	40.0	33.0	41.0
29	36.534560687288305	37.0	35.0	40.0	33.0	41.0
30	36.21025212534488	37.0	35.0	40.0	33.0	41.0
31	35.64704134048137	37.0	35.0	40.0	31.0	41.0
32	34.91898828349895	37.0	35.0	40.0	24.0	41.0
33	33.97814465056115	37.0	34.0	40.0	17.0	41.0
34	33.159888741840696	37.0	33.0	40.0	12.0	41.0
35	32.702384423857694	37.0	33.0	40.0	10.0	41.0
36	32.51759531339958	37.0	33.0	40.0	9.0	41.0
37	32.27723320098996	37.0	31.0	40.0	8.0	41.0
38	32.170121053064456	37.0	31.0	40.0	8.0	41.0
39	32.15258181736614	37.0	31.0	40.0	8.0	41.0
40	31.986434804064512	36.0	31.0	40.0	8.0	41.0
41	31.85419610745983	36.0	30.0	40.0	8.0	41.0
42	31.739698526278012	36.0	30.0	40.0	7.0	41.0
43	31.60182588995312	36.0	30.0	40.0	7.0	41.0
44	31.4433615217245	36.0	29.0	40.0	7.0	41.0
45	31.43874071914044	36.0	29.0	40.0	7.0	41.0
46	31.393345819968147	36.0	29.0	40.0	7.0	41.0
47	31.37361955392058	36.0	29.0	40.0	7.0	41.0
48	31.333466050559654	36.0	29.0	40.0	7.0	41.0
49	31.22452203105957	36.0	27.0	40.0	7.0	41.0
50	31.094799353984882	35.0	27.0	40.0	7.0	41.0
51	30.5552944827019	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	2.0
12	5.0
13	8.0
14	15.0
15	16.0
16	37.0
17	98.0
18	166.0
19	360.0
20	620.0
21	1048.0
22	1772.0
23	3064.0
24	5753.0
25	12188.0
26	21333.0
27	26338.0
28	23701.0
29	18052.0
30	14398.0
31	12418.0
32	13171.0
33	16594.0
34	30080.0
35	44372.0
36	41351.0
37	51500.0
38	94363.0
39	102090.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.700754432007656	16.88536970159186	18.380027365918217	36.03384850048227
2	48.84050006355473	18.775562085492325	18.99856441084767	13.385373440105278
3	22.30266256925596	18.456853816648348	46.18372550339083	13.056758110704859
4	20.75117950098323	19.164180555244013	46.247467157159626	13.83717278661313
5	18.6963056010408	20.91436561165818	45.27021974981868	15.119109037482337
6	18.5893841172996	23.870408170894926	44.62850392170058	12.911703790104903
7	56.842788033766254	5.219712433547924	33.26136695004598	4.676132582639839
8	56.38463321444861	4.415184346096618	33.23856201819908	5.961620421255692
9	52.14740210702617	6.118637984791728	35.51232587873758	6.221634029444532
10	29.288822592584285	18.706025735926364	41.04214800026917	10.963003671220175
11	21.9979737257277	18.560784489655532	44.47615949993644	14.96508228468032
12	20.080116342537554	16.236737623651333	48.62254473131304	15.060601302498075
13	16.403662247743807	21.882640586797066	48.32907142803736	13.384625737421771
14	12.246809178798143	24.55044376154266	47.47893347689225	15.72381358276695
15	10.976088468181512	20.579768660789725	53.49588389672731	14.94825897430146
16	12.42756630253546	19.65560814397763	50.5882550862475	17.328570467239405
17	12.365320054133674	19.707760406152097	46.208773543288245	21.71814599642598
18	14.332712740106023	19.752435641491516	49.09172816521238	16.82312345319007
19	15.076489984522553	20.995117501476713	46.59496197931855	17.33343053468219
20	16.88593047860449	21.127834727798838	47.0200309548911	14.966203838705578
21	15.43594804961755	21.053064459448343	47.461175538159004	16.049811952775098
22	13.668565831482768	21.069140067143703	45.91062709824066	19.351667003132874
23	13.353222224714564	21.244476346425607	46.04521358127155	19.357087847588286
24	16.097664924519414	18.930149615306966	46.9185303156053	18.053655144568314
25	13.263310977023096	20.81753811414429	45.99885601489424	19.920294893938376
26	12.663279573510389	22.48977516580307	47.24135094920855	17.605594311477983
27	16.383661200960052	21.55047366965	46.87572433697464	15.190140792415304
28	12.591313190223039	19.761034222351824	50.02916040465669	17.618492182768446
29	13.215271079607904	18.611628272133867	48.98088124238278	19.192219405875445
30	16.36795944460645	21.764503562803288	45.06834002527235	16.79919696731792
31	15.553150445256946	24.339778530465146	43.31460338111153	16.79246764316637
32	16.000276649992898	24.974578108760834	40.9703685426527	18.05477669859357
33	18.524520909505544	25.399647084333388	38.415281547445474	17.660550458715598
34	14.968073095414338	25.162438407991445	40.58586243766029	19.283626058933926
35	15.889803578505044	25.570310221843386	36.736315171635155	21.803571028016417
36	20.455089238315278	25.89724322020592	36.56322200040376	17.08444554107505
37	17.87327187217275	26.992814577211515	38.37490560253621	16.759007948079528
38	16.347584546480938	28.613833995050207	34.330955638799786	20.70762581966907
39	18.54265269958054	26.85654576314274	35.44185490081724	19.15894663645948
40	18.74864478888615	27.321990683624563	35.848792086314795	18.080572441174493
41	17.783360624481283	27.406481086860623	33.50324876815983	21.30690952049827
42	20.236760054731835	24.75082808072198	35.16314872554078	19.849263139005405
43	21.62001001921596	23.70198814143544	34.864254577809675	19.81374726153892
44	17.678495323119712	25.028412701973185	34.83621572717824	22.45687624772885
45	16.807982473849098	25.09757520019739	33.510538869324	24.583903456629503
46	19.37372423229627	25.80546271580569	35.42428388775487	19.39652916414317
47	15.906626888883904	25.814622073678624	38.26966644983289	20.009084587604583
48	16.89976297824933	23.330940684746118	37.130728337184	22.638567999820552
49	18.601347360235675	21.175500773872276	39.16018034588726	21.062971520004787
50	17.12594304000957	21.571035493446384	38.40612218957254	22.896899276971507
51	15.604555004747912	22.29238165735777	34.53563924840926	27.56742408948506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	745.5
2	859.0
3	20475.5
4	40092.0
5	26719.0
6	13346.0
7	13157.0
8	12968.0
9	13085.5
10	13203.0
11	12988.5
12	12774.0
13	12139.5
14	11505.0
15	10554.0
16	9603.0
17	8688.5
18	7774.0
19	7199.0
20	6624.0
21	6085.0
22	5546.0
23	5100.0
24	4654.0
25	4605.5
26	4725.5
27	4894.0
28	5070.0
29	5246.0
30	5950.0
31	6654.0
32	7044.0
33	7434.0
34	8173.0
35	8912.0
36	9782.0
37	10652.0
38	11758.0
39	12864.0
40	16206.5
41	19549.0
42	24717.5
43	29886.0
44	38179.0
45	46472.0
46	53433.5
47	60395.0
48	57744.5
49	55094.0
50	44247.0
51	33400.0
52	27782.0
53	22164.0
54	19227.5
55	16291.0
56	14172.5
57	12054.0
58	11104.5
59	10155.0
60	9213.5
61	8272.0
62	8284.5
63	8297.0
64	6467.0
65	4637.0
66	3745.5
67	2854.0
68	2293.0
69	1732.0
70	1415.5
71	1099.0
72	873.0
73	647.0
74	639.5
75	413.5
76	195.0
77	178.5
78	162.0
79	145.5
80	129.0
81	88.0
82	47.0
83	27.0
84	7.0
85	6.5
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	534972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.27816349993688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7869786209334	28.251993801973757
2	10.625718908930153	7.922145735829384
3	4.338309135871307	4.851725918408413
4	2.2461433660273764	3.349283985722684
5	1.2627867946003326	2.3537186297336206
6	0.8466420224498663	1.893675584328201
7	0.5403032635735762	1.4099059379331682
8	0.3807891007568804	1.135609468560714
9	0.3302689780056054	1.1080638864945014
>10	3.2462725973817768	28.086085973968295
>50	0.32215238142886465	7.523699278968361
>100	0.06698724121773733	4.405678829853647
>500	0.0025567649319747075	0.5242132564078198
>1k	0.0035794709047645905	2.7760427829334833
>5k	0.0	0.0
>10k+	5.113529863949415E-4	4.4081569288839395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23125	4.322656139012883	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3971	0.7422818390495203	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3263	0.6099384640691475	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2155	0.40282482073828163	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1577	0.2947817829718191	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1311	0.24505955451874115	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1270	0.23739560201281562	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1016	0.1899164816102525	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	602	0.11252925386749213	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	572	0.10692148374120515	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	535	0.10000523391878453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8692567087623277E-4	0.0	0.0	0.4267513066104394	0.0
2	1.8692567087623277E-4	0.0	0.0	2.393022438557532	0.0
3	1.8692567087623277E-4	0.0	0.0	3.3250338335464287	0.0
4	1.8692567087623277E-4	0.0	0.0	4.230875634612652	0.0
5	1.8692567087623277E-4	0.0	0.0	6.435476996926942	0.0
6	1.8692567087623277E-4	0.0	0.0	7.525627509477132	0.0
7	1.8692567087623277E-4	0.0	0.0	8.752046836096095	0.0
8	1.8692567087623277E-4	0.0	0.0	10.554010303342979	0.0
9	1.8692567087623277E-4	0.0	0.0	11.199090793536858	0.0
10	1.8692567087623277E-4	0.0	0.0	12.57691991356557	0.0
11	1.8692567087623277E-4	0.0	0.0	14.771988066665172	0.0
12	1.8692567087623277E-4	0.0	0.0	16.48927420500512	0.0
13	1.8692567087623277E-4	0.0	0.0	17.088370980163447	0.0
14	1.8692567087623277E-4	0.0	0.0	17.319224183695596	0.0
15	1.8692567087623277E-4	0.0	0.0	17.737003058103976	0.0
16	1.8692567087623277E-4	0.0	0.0	18.700604891470956	0.0
17	1.8692567087623277E-4	0.0	0.0	19.917677934546106	0.0
18	1.8692567087623277E-4	0.0	0.0	21.397942322214995	0.0
19	1.8692567087623277E-4	0.0	0.0	22.15181355285884	0.0
20	1.8692567087623277E-4	0.0	0.0	22.823063636975395	0.0
21	1.8692567087623277E-4	0.0	0.0	23.640676521388034	0.0
22	1.8692567087623277E-4	0.0	0.0	24.53511585653081	0.0
23	1.8692567087623277E-4	0.0	0.0	25.284127019731873	0.0
24	1.8692567087623277E-4	0.0	0.0	25.924534368153846	0.0
25	1.8692567087623277E-4	0.0	0.0	26.45671175313848	0.0
26	1.8692567087623277E-4	0.0	0.0	26.955055591694517	0.0
27	1.8692567087623277E-4	0.0	0.0	27.450034768174785	0.0
28	1.8692567087623277E-4	0.0	0.0	27.94090157989577	0.0
29	1.8692567087623277E-4	0.0	0.0	28.44896555333737	0.0
30	1.8692567087623277E-4	0.0	0.0	29.051987767584098	0.0
31	5.607770126286984E-4	0.0	0.0	29.617811773326455	0.0
32	5.607770126286984E-4	0.0	0.0	30.117837942920378	0.0
33	5.607770126286984E-4	0.0	0.0	30.558608674846536	0.0
34	5.607770126286984E-4	0.0	0.0	31.032278324846907	0.0
35	5.607770126286984E-4	0.0	0.0	31.525014393276656	0.0
36	5.607770126286984E-4	0.0	0.0	32.02672289390846	0.0
37	7.477026835049311E-4	0.0	0.0	32.495158625124304	0.0
38	7.477026835049311E-4	0.0	0.0	32.91480675624145	0.0
39	7.477026835049311E-4	0.0	0.0	33.34324039388977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1633168E-6	45.000004	1
CGGGTCG	25	3.8879993E-5	45.000004	6
CGGAACG	25	3.8879993E-5	45.000004	1
TCGTTAG	25	3.8879993E-5	45.000004	1
ATCTAAT	30	2.1633168E-6	45.000004	19
CAGCATA	30	2.1633168E-6	45.000004	26
GACGTCC	25	3.8879993E-5	45.000004	19
CAACAGG	130	0.0	45.000004	2
CCGCTAC	25	3.8879993E-5	45.000004	20
CCAGACG	25	3.8879993E-5	45.000004	16
GCCGCTA	25	3.8879993E-5	45.000004	19
TTGAGTA	25	3.8879993E-5	45.000004	23
ATCGTAG	30	2.1633168E-6	45.000004	1
ATGTACG	30	2.1633168E-6	45.000004	1
CTAACCG	30	2.1633168E-6	45.000004	1
TACGCGG	25	3.8879993E-5	45.000004	2
TAACCGG	30	2.1633168E-6	45.000004	2
TGACCGG	25	3.8879993E-5	45.000004	2
TATCTAG	25	3.8879993E-5	45.000004	36
AGCGATT	25	3.8879993E-5	45.000004	22
>>END_MODULE