##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934900.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785702
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33423486258149	33.0	31.0	34.0	30.0	34.0
2	32.51942848560905	34.0	31.0	34.0	31.0	34.0
3	32.60815550934069	34.0	31.0	34.0	31.0	34.0
4	36.04292212569142	37.0	35.0	37.0	35.0	37.0
5	35.90894893992888	37.0	35.0	37.0	35.0	37.0
6	35.885520209952375	37.0	35.0	37.0	35.0	37.0
7	36.09545476529269	37.0	35.0	37.0	35.0	37.0
8	36.10855387920611	37.0	36.0	37.0	35.0	37.0
9	37.90093317822788	39.0	38.0	39.0	35.0	39.0
10	37.3907053819387	39.0	37.0	39.0	34.0	39.0
11	37.26585779341277	39.0	37.0	39.0	34.0	39.0
12	37.27988601276311	39.0	37.0	39.0	34.0	39.0
13	37.1887369511596	39.0	37.0	39.0	33.0	39.0
14	38.361063100259386	40.0	38.0	41.0	34.0	41.0
15	38.43863704050645	40.0	38.0	41.0	34.0	41.0
16	38.55268918750366	40.0	38.0	41.0	34.0	41.0
17	38.574795533166515	40.0	38.0	41.0	34.0	41.0
18	38.14958852083869	39.0	38.0	40.0	34.0	41.0
19	37.723728589210666	38.0	37.0	40.0	34.0	41.0
20	37.21136894140527	38.0	35.0	40.0	34.0	41.0
21	37.13212515686609	38.0	35.0	40.0	34.0	41.0
22	36.924132813713086	38.0	35.0	40.0	33.0	41.0
23	36.805152589658675	38.0	35.0	40.0	33.0	41.0
24	36.574786623936305	37.0	35.0	40.0	33.0	41.0
25	36.4245655477522	37.0	35.0	40.0	33.0	41.0
26	36.63487301801447	37.0	35.0	40.0	33.0	41.0
27	36.715454459833374	38.0	35.0	40.0	33.0	41.0
28	36.717036484570485	38.0	35.0	40.0	33.0	41.0
29	36.64824831806461	38.0	35.0	40.0	33.0	41.0
30	36.36748029151001	38.0	35.0	40.0	33.0	41.0
31	35.92587520459411	38.0	35.0	40.0	31.0	41.0
32	35.31108868247758	38.0	35.0	40.0	27.0	41.0
33	34.378727558285455	38.0	35.0	40.0	18.0	41.0
34	33.73561732056174	38.0	34.0	40.0	15.0	41.0
35	33.329048417847986	38.0	34.0	40.0	10.0	41.0
36	33.08137309056105	38.0	33.0	40.0	10.0	41.0
37	32.90229119946239	38.0	33.0	40.0	10.0	41.0
38	32.77588322290131	38.0	33.0	40.0	10.0	41.0
39	32.7197678000056	38.0	33.0	40.0	10.0	41.0
40	32.62909729133946	38.0	32.0	40.0	10.0	41.0
41	32.49918416906155	37.0	32.0	40.0	8.0	41.0
42	32.32441816362947	37.0	31.0	40.0	8.0	41.0
43	32.199365662808546	37.0	31.0	40.0	8.0	41.0
44	32.079197965640915	37.0	31.0	40.0	8.0	41.0
45	32.07490117118195	37.0	31.0	40.0	8.0	41.0
46	32.00354841912073	37.0	31.0	40.0	8.0	41.0
47	31.95868535398917	37.0	30.0	40.0	8.0	41.0
48	31.926318120610613	37.0	30.0	40.0	8.0	41.0
49	31.83185482536636	37.0	30.0	40.0	8.0	41.0
50	31.756004184792708	37.0	30.0	40.0	8.0	41.0
51	31.17835006147369	35.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	7.0
11	5.0
12	5.0
13	10.0
14	16.0
15	40.0
16	73.0
17	127.0
18	261.0
19	532.0
20	908.0
21	1589.0
22	2397.0
23	4339.0
24	7961.0
25	16486.0
26	28580.0
27	35320.0
28	31373.0
29	24266.0
30	19895.0
31	18567.0
32	19747.0
33	24850.0
34	43084.0
35	61793.0
36	63481.0
37	80325.0
38	148510.0
39	151076.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.83039625710511	16.875100228839944	20.159297036280933	34.13520647777402
2	45.834553049374954	19.98887618970042	19.957821158658117	14.21874960226651
3	22.54124337216909	18.392978508391224	45.729933231683255	13.335844887756426
4	21.067783968985694	19.943311840876056	44.4050034236899	14.583900766448348
5	18.51999867634294	22.232602182506852	44.00408806392245	15.243311077227753
6	19.779509279599644	24.3358168873186	43.02980010232888	12.85487373075288
7	59.21328951689063	4.670727578649412	31.41534576722472	4.700637137235237
8	58.93404878694467	3.5421826595834047	30.963647795220073	6.560120758251857
9	54.0895912190627	5.573741698506558	33.54872458005707	6.787942502373673
10	27.725269886038216	20.358991067860334	39.7322394495623	12.183499596539146
11	19.768690928621794	19.49390481378436	44.76875456598049	15.96864969161336
12	18.664200931141835	16.179162074170613	49.3046982189176	15.851938775769947
13	16.082306014239496	21.553464290532542	48.719107244222364	13.645122451005598
14	12.390830111161739	24.84937037197309	45.76926111935569	16.99053839750949
15	11.441869818328069	21.56364626792346	52.135160658875755	14.859323254872713
16	12.802691096624418	21.306805888237527	48.27708215073908	17.61342086439897
17	12.549159859590532	20.926763582121467	44.704862657852466	21.819213900435532
18	13.337117634930292	20.108514424043722	49.490264756867106	17.06410318415888
19	14.65415641044569	21.194804136937414	45.82806203878824	18.322977413828653
20	15.797719746163303	22.14427352864063	46.10488454910386	15.953122176092208
21	15.259602241053225	22.30489422198238	47.16444657134639	15.271056965618007
22	13.783851892956871	21.41868036482025	44.27314681647749	20.524320925745386
23	12.972475569617997	21.433698781471854	45.55314864923343	20.04067699967672
24	16.305927692687558	19.420085477700198	46.220195443056014	18.053791386556224
25	12.363466046923644	21.765885793850597	45.09483239192467	20.77581576730109
26	11.90744073452785	22.194419767290906	45.56320335190696	20.334936146274288
27	14.134748288791425	21.667502437310837	46.79484079205602	17.402908481841717
28	11.069463995255198	20.340918057991452	46.883042171204856	21.70657577554849
29	11.691200989688202	19.019170117932756	45.407928196695444	23.881700695683605
30	14.031019394121437	22.084072587316818	44.340602416692334	19.54430560186941
31	16.44656625539963	22.234765852702424	39.24286816121125	22.0757997306867
32	16.51567642694049	21.8534507994125	39.296068993078805	22.334803780568205
33	15.83666580968357	23.19263537575315	40.142064039546796	20.828634775016482
34	16.4204749383354	21.281732768912388	38.7676243664901	23.53016792626212
35	16.6550422424787	21.588210288379052	36.18967496582674	25.567072503315508
36	19.005933547324556	22.483969749345174	37.86639209267636	20.643704610653913
37	17.64969415885412	22.789174521637975	38.46585601156672	21.09527530794118
38	16.54851330402621	26.007697574907535	34.44639316178398	22.997395959282272
39	18.516180434821344	23.92471955016024	36.883958549170046	20.675141465848377
40	21.718157774830658	22.611244466731662	35.79283239701566	19.877765361422014
41	18.46921606410573	23.43623918483089	34.3557735630048	23.73877118805858
42	18.726056443791666	21.984925582472744	36.43989706020857	22.84912091352701
43	19.296374452400528	21.93147020117042	36.14283786982851	22.629317476600544
44	16.29689118775312	23.643315150018708	34.33617325652729	25.723620405700885
45	17.357216858299967	22.935540446632437	32.84145897554034	26.865783719527254
46	20.453938007030654	23.072360767822914	35.07309387019506	21.40060735495137
47	16.691824635803396	22.354658636480497	38.73147834675233	22.222038380963774
48	17.23414220658723	20.30260836805812	37.39623928665066	25.06701013870399
49	17.779005271718795	18.959351000761103	40.47908749118623	22.78255623633388
50	17.88107959506276	19.281228761031535	38.160905788708696	24.676785855197007
51	16.508294493332077	19.863256043639954	34.65028725903714	28.978162203990827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	759.0
1	788.0
2	817.0
3	26625.5
4	52434.0
5	34927.0
6	17420.0
7	17185.5
8	16951.0
9	17379.5
10	17808.0
11	17411.0
12	17014.0
13	16075.5
14	15137.0
15	14062.5
16	12988.0
17	11893.0
18	10798.0
19	9901.0
20	9004.0
21	8418.0
22	7832.0
23	7278.0
24	6724.0
25	7366.0
26	8090.0
27	8172.0
28	8402.5
29	8633.0
30	10170.0
31	11707.0
32	12749.0
33	13791.0
34	13988.0
35	14185.0
36	14594.5
37	15004.0
38	17145.0
39	19286.0
40	21761.5
41	24237.0
42	28272.5
43	32308.0
44	37190.0
45	42072.0
46	45874.0
47	49676.0
48	58386.0
49	67096.0
50	77103.5
51	87111.0
52	84205.0
53	81299.0
54	63016.5
55	44734.0
56	35028.5
57	25323.0
58	20563.0
59	15803.0
60	13185.5
61	10568.0
62	9318.5
63	8069.0
64	6616.5
65	5164.0
66	4271.5
67	3379.0
68	2635.5
69	1892.0
70	1442.0
71	992.0
72	862.0
73	732.0
74	538.0
75	249.5
76	155.0
77	112.0
78	69.0
79	117.0
80	165.0
81	94.5
82	24.0
83	16.0
84	8.0
85	6.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.606280284833765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2219839478958	25.685467797973143
2	10.746501833297812	7.4379290904916795
3	4.623712264776695	4.8002844777385745
4	2.5126235647032797	3.4781022132159936
5	1.5829887552149913	2.7390676275355053
6	1.002027263516894	2.0805861800586376
7	0.7111489159593875	1.7227153096962378
8	0.4889565532632848	1.3536774023468388
9	0.3837324222658223	1.195159658337831
>10	3.134328231456472	25.126566371958088
>50	0.5152351918255366	11.528839555082811
>100	0.06814883980479683	4.470681002755607
>500	0.005242218446522831	1.1935724389548679
>1k	0.0022466650485097844	1.5216720389368161
>5k	7.488883495032615E-4	1.5709128951742333
>10k+	3.7444417475163074E-4	4.094765939743114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31600	4.021881069413085	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	6336	0.806412609360801	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	5787	0.7365387895156178	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	3225	0.41046096357143036	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2622	0.33371430898737686	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1807	0.2299854143173875	No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1626	0.20694869047043282	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	1461	0.18594836210166196	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1002	0.12752926682126303	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	877	0.11161992714795177	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	873	0.11111082827840581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2727471738649003E-4	0.0	0.0	0.4359159070487284	0.0
2	2.5454943477298006E-4	0.0	0.0	2.6726417903989046	0.0
3	2.5454943477298006E-4	0.0	0.0	3.676075662273992	0.0
4	2.5454943477298006E-4	0.0	0.0	4.725455707125603	0.0
5	2.5454943477298006E-4	0.0	0.0	7.202603531618858	0.0
6	2.5454943477298006E-4	0.0	0.0	8.43653191668088	0.0
7	2.5454943477298006E-4	0.0	0.0	9.743770538957518	0.0
8	2.5454943477298006E-4	0.0	0.0	11.72938340490415	0.0
9	2.5454943477298006E-4	0.0	0.0	12.468340414050111	0.0
10	2.5454943477298006E-4	0.0	0.0	14.075692819924093	0.0
11	2.5454943477298006E-4	0.0	0.0	16.361419469468068	0.0
12	2.5454943477298006E-4	0.0	0.0	18.145938281944044	0.0
13	3.8182415215947015E-4	0.0	0.0	18.814894196527437	0.0
14	3.8182415215947015E-4	0.0	0.0	19.052770643322788	0.0
15	3.8182415215947015E-4	0.0	0.0	19.504595890044826	0.0
16	3.8182415215947015E-4	0.0	0.0	20.556521429244167	0.0
17	3.8182415215947015E-4	0.0	0.0	21.947124991408955	0.0
18	3.8182415215947015E-4	0.0	0.0	23.6302058541279	0.0
19	3.8182415215947015E-4	0.0	0.0	24.477473647769767	0.0
20	3.8182415215947015E-4	0.0	0.0	25.252322127218715	0.0
21	3.8182415215947015E-4	0.0	0.0	26.19631870607431	0.0
22	3.8182415215947015E-4	0.0	0.0	27.127460538473873	0.0
23	3.8182415215947015E-4	0.0	0.0	28.039256613830688	0.0
24	3.8182415215947015E-4	0.0	0.0	28.72997650508717	0.0
25	3.8182415215947015E-4	0.0	0.0	29.361259103324162	0.0
26	3.8182415215947015E-4	0.0	0.0	29.946595528584627	0.0
27	3.8182415215947015E-4	0.0	0.0	30.507240658672117	0.0
28	3.8182415215947015E-4	0.0	0.0	31.056685613629593	0.0
29	3.8182415215947015E-4	0.0	0.0	31.615039798804126	0.0
30	5.090988695459601E-4	0.0	0.0	32.24721332006282	0.0
31	5.090988695459601E-4	0.0	0.0	32.86296840277866	0.0
32	5.090988695459601E-4	0.0	0.0	33.41724979699683	0.0
33	5.090988695459601E-4	0.0	0.0	33.97267666367147	0.0
34	5.090988695459601E-4	0.0	0.0	34.47808456641322	0.0
35	5.090988695459601E-4	0.0	0.0	35.0218021590883	0.0
36	5.090988695459601E-4	0.0	0.0	35.53446472072109	0.0
37	5.090988695459601E-4	0.0	0.0	36.05043642500592	0.0
38	5.090988695459601E-4	0.0	0.0	36.55660797605199	0.0
39	5.090988695459601E-4	0.0	0.0	37.02319708999086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	35	1.211265E-7	45.000004	1
AACGCCG	35	1.211265E-7	45.000004	13
ACTAACG	30	2.164621E-6	45.000004	24
CATCGTT	35	1.211265E-7	45.000004	36
TCGAGAG	30	2.164621E-6	45.000004	1
TCTGGTA	35	1.211265E-7	45.000004	43
GGCTATC	35	1.211265E-7	45.000004	8
CGAAGTA	30	2.164621E-6	45.000004	27
AGATGCG	35	1.211265E-7	45.000004	1
GTTTGCG	25	3.889675E-5	45.0	1
AGATCCG	20	7.031924E-4	45.0	27
GCAAGCG	40	6.8102963E-9	45.0	1
ACACGAC	20	7.031924E-4	45.0	10
GTCGAGC	20	7.031924E-4	45.0	20
TTTACGA	25	3.889675E-5	45.0	16
ACCGCGT	20	7.031924E-4	45.0	37
TAGGTCG	20	7.031924E-4	45.0	1
CATTGCG	25	3.889675E-5	45.0	1
GCCGGTT	20	7.031924E-4	45.0	39
TGTATCG	20	7.031924E-4	45.0	1
>>END_MODULE