SRR2934899.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1980316
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72253	3.648559118847699	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5964	0.30116405664550505	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5149	0.2600090086632638	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	4405	0.2224392470696596	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	3689	0.18628340123495443	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	2210	0.11159835097024919	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2099	0.10599318492604212	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1985	0.10023652790766727	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATG	25	3.8918304E-5	45.000004	1
ATAGCGT	20	7.0345245E-4	45.0	13
AATGCGC	20	7.0345245E-4	45.0	44
CGTTTTT	53260	0.0	44.573322	1
TATAGCG	65	0.0	41.53846	1
GTTTTTT	60155	0.0	39.542847	2
TTGCGAG	100	0.0	38.250004	1
ATAGCGG	225	0.0	38.000004	2
TAGTGCG	125	0.0	37.800003	1
TACCCGT	30	1.14022E-4	37.500004	32
CTGCGAA	30	1.14022E-4	37.500004	44
AATAGCG	60	1.5643309E-10	37.500004	1
CGAGATA	205	0.0	37.317074	19
AACGTAG	110	0.0	36.81818	1
TTAGCCG	55	2.752131E-9	36.81818	1
TCGATAG	110	0.0	36.81818	1
TCATCGA	55	2.752131E-9	36.81818	16
TATCGAG	55	2.752131E-9	36.81818	1
TTCGTAG	80	0.0	36.5625	1
CCCTACG	25	0.0021074754	36.000004	32