##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1980316
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45630394341105	34.0	31.0	34.0	30.0	34.0
2	32.63804867505994	34.0	31.0	34.0	31.0	34.0
3	32.692777819297525	34.0	31.0	34.0	31.0	34.0
4	36.12663635500597	37.0	37.0	37.0	35.0	37.0
5	35.995127040330935	37.0	35.0	37.0	35.0	37.0
6	35.97209788740787	37.0	35.0	37.0	35.0	37.0
7	36.181421550904	37.0	36.0	37.0	35.0	37.0
8	36.20974379846449	37.0	37.0	37.0	35.0	37.0
9	38.0104756008637	39.0	39.0	39.0	35.0	39.0
10	37.38882430884768	39.0	37.0	39.0	34.0	39.0
11	37.348942290018364	39.0	37.0	39.0	34.0	39.0
12	37.438237634801716	39.0	37.0	39.0	35.0	39.0
13	37.45579947846707	39.0	37.0	39.0	35.0	39.0
14	38.74468014195714	40.0	38.0	41.0	35.0	41.0
15	38.76824658286859	40.0	38.0	41.0	35.0	41.0
16	38.84693806443012	40.0	38.0	41.0	35.0	41.0
17	38.85602600797044	40.0	38.0	41.0	35.0	41.0
18	38.36483621805813	39.0	38.0	41.0	35.0	41.0
19	37.8637338687361	38.0	37.0	40.0	35.0	41.0
20	37.31363933836822	38.0	35.0	40.0	34.0	41.0
21	37.256694891118386	38.0	35.0	40.0	34.0	41.0
22	37.07800472247863	38.0	35.0	40.0	34.0	41.0
23	36.94881978431725	38.0	35.0	40.0	33.0	41.0
24	36.72299976367408	38.0	35.0	40.0	33.0	41.0
25	36.585936789886055	38.0	35.0	40.0	33.0	41.0
26	36.74828613211225	38.0	35.0	40.0	33.0	41.0
27	36.79831451142141	38.0	35.0	40.0	33.0	41.0
28	36.75292074598195	38.0	35.0	40.0	33.0	41.0
29	36.652696842322136	38.0	35.0	40.0	33.0	41.0
30	36.34115666388597	38.0	35.0	40.0	32.0	41.0
31	35.84775662066054	38.0	35.0	40.0	30.0	41.0
32	35.12227391991985	38.0	35.0	40.0	23.0	41.0
33	34.12259306090543	38.0	34.0	40.0	16.0	41.0
34	33.29390662904304	38.0	33.0	40.0	12.0	41.0
35	32.74011723381521	38.0	32.0	40.0	9.0	41.0
36	32.48844224861083	38.0	31.0	40.0	8.0	41.0
37	32.26994025195979	38.0	31.0	40.0	8.0	41.0
38	32.160520846167984	38.0	30.0	40.0	7.0	41.0
39	32.122193629703546	38.0	30.0	40.0	7.0	41.0
40	32.027498641630935	38.0	30.0	40.0	7.0	41.0
41	31.905957938026052	38.0	29.0	40.0	7.0	41.0
42	31.77897870844855	38.0	29.0	40.0	7.0	41.0
43	31.681151392000064	38.0	28.0	40.0	7.0	41.0
44	31.577550754526044	38.0	27.0	40.0	7.0	41.0
45	31.52852322558622	37.0	26.0	40.0	7.0	41.0
46	31.44807343878452	37.0	26.0	40.0	7.0	41.0
47	31.40736225935659	37.0	26.0	40.0	7.0	41.0
48	31.34170354630271	37.0	26.0	40.0	7.0	41.0
49	31.215052042199325	37.0	26.0	40.0	7.0	41.0
50	31.126906008939986	37.0	25.0	40.0	7.0	41.0
51	30.513441289167993	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	9.0
10	14.0
11	14.0
12	18.0
13	25.0
14	30.0
15	73.0
16	121.0
17	304.0
18	598.0
19	1285.0
20	2325.0
21	3777.0
22	6276.0
23	10968.0
24	22290.0
25	48754.0
26	85966.0
27	101162.0
28	88780.0
29	69233.0
30	54531.0
31	49034.0
32	47925.0
33	56168.0
34	87946.0
35	113821.0
36	134734.0
37	191893.0
38	368293.0
39	433713.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.863128914779256	16.946234843328035	20.072655071210857	35.11798117068185
2	49.871333665940185	18.442359704208826	19.172950175628536	12.513356454222457
3	22.047440913470375	18.25582381801692	46.78768438976405	12.909050878748642
4	20.57398920172336	18.990605539721944	46.55029803324318	13.885107225311517
5	18.524922285130252	21.247467575881828	45.85217712728676	14.375433011701164
6	18.766348400962272	25.049689039527024	44.66665926044126	11.517303299069441
7	60.038902882166276	3.966134697694711	32.2369763209508	3.7579860991882104
8	61.406563396952805	3.0277491067082223	31.703223121966396	3.862464374372575
9	57.13618432613785	4.6447132679834935	33.24146247366582	4.977639932212838
10	26.985188222485707	19.8365816364661	40.71506769626666	12.463162444781538
11	19.77654071370428	20.098560027793543	45.06412107966607	15.060778178836104
12	18.412919958228887	17.162008487534315	48.57275303537415	15.852318518862646
13	16.997085313657013	18.177250499415244	50.186838868140235	14.638825318787507
14	14.932566317698793	20.782087303238473	48.229020014987505	16.05632636407523
15	13.954793073428684	20.22444902732695	50.5554164082904	15.265341490953968
16	15.81156744681152	19.67913201731441	48.90406379587905	15.605236739995032
17	16.050771695022412	19.685797620177787	46.61932742047229	17.644103264327512
18	16.582303026385688	20.029530640564435	48.01400382565207	15.37416250739781
19	15.928114502937914	21.485055920368264	46.90857418714993	15.67825538954389
20	17.238056956566528	20.824151297065722	46.99037931320052	14.947412433167232
21	16.30027732947671	20.811072576295906	48.23230231942781	14.656347774799578
22	15.848278759551507	19.646712948842506	46.57004235687638	17.934965934729608
23	14.852579083338217	20.62201184053454	47.05526794713571	17.470141128991536
24	15.636292389699422	19.31176640495759	47.800906521989425	17.251034683353566
25	14.952159150357822	21.19328430412116	45.80678033202782	18.0477762134932
26	14.919033124006473	21.58599940615538	47.17156251830516	16.32340495153299
27	15.454907196629225	20.849046313820622	47.437075699029855	16.2589707905203
28	14.054221649474124	20.38886723129036	48.52942661676217	17.027484502473342
29	16.68067116561195	18.87047319720691	47.19660902603423	17.25224661114691
30	16.302145718158112	19.713621462433267	47.471615641140104	16.512617178268517
31	15.676639485819436	20.917217252196114	45.38977617713537	18.016367084849087
32	17.08303119300152	21.229339155973086	43.461094088014235	18.226535563011158
33	16.170803043554667	21.299681464978317	43.1022624672022	19.427253024264814
34	16.44813252026444	21.797278818128017	40.91271292056419	20.841875741043346
35	17.819782297370722	22.380519068673888	38.41326333777034	21.386435296185052
36	17.321477986341574	24.316927197477575	38.25182445629889	20.10977035988196
37	17.4717065357246	24.34833632612169	38.76305599712369	19.416901141030017
38	17.41035269118666	24.780136099491195	37.007932067407424	20.801579141914726
39	18.343789577017	23.65460865841613	37.05418731151998	20.947414453046886
40	18.512096049317382	23.15100216329111	37.28031283896105	21.056588948430452
41	17.130700352872978	24.094386956425137	35.36506294954946	23.40984974115242
42	18.13432805673438	23.513318076509	36.12660807669079	22.22574579006583
43	18.704136107570708	23.41611136808469	36.7064145318222	21.173337992522406
44	18.26885204179535	23.43358332710537	36.31521433952965	21.982350291569627
45	19.058069520217984	22.316286895626757	36.005415297356585	22.62022828679867
46	18.85365769907429	22.71440517573963	36.84972499338489	21.58221213180119
47	16.481006061658846	22.638407203698804	39.028670171831166	21.851916562811187
48	16.886042429592045	21.501618933543938	38.34721327303319	23.265125363830823
49	18.028940835705008	20.619587984947856	39.225305456300916	22.12616572304622
50	17.222301895253082	20.128201761739035	39.092144890007454	23.55735145300043
51	16.848523164989828	20.265503081326415	36.86603552160362	26.01993823208013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	519.0
1	844.5
2	1170.0
3	70802.0
4	140434.0
5	98322.0
6	56210.0
7	53374.0
8	50538.0
9	49395.5
10	48253.0
11	46703.0
12	45153.0
13	43162.5
14	41172.0
15	38241.5
16	35311.0
17	32534.0
18	29757.0
19	27317.5
20	24878.0
21	23139.5
22	21401.0
23	19919.0
24	18437.0
25	18287.5
26	18748.0
27	19358.0
28	20851.0
29	22344.0
30	24107.5
31	25871.0
32	29664.0
33	33457.0
34	37314.0
35	41171.0
36	45053.0
37	48935.0
38	53388.5
39	57842.0
40	64308.5
41	70775.0
42	76177.0
43	81579.0
44	89583.5
45	97588.0
46	106613.0
47	115638.0
48	135786.5
49	155935.0
50	169319.5
51	182704.0
52	176212.5
53	169721.0
54	137911.5
55	106102.0
56	86981.5
57	67861.0
58	57611.5
59	47362.0
60	42469.5
61	37577.0
62	32239.0
63	26901.0
64	22324.5
65	17748.0
66	13800.0
67	9852.0
68	7646.0
69	5440.0
70	4229.0
71	3018.0
72	2467.0
73	1916.0
74	1577.0
75	853.5
76	469.0
77	356.0
78	243.0
79	210.5
80	178.0
81	111.5
82	45.0
83	51.5
84	58.0
85	34.5
86	11.0
87	7.0
88	3.0
89	4.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1980316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.69444261849589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59190736118391	28.870892570673
2	10.481630365784104	7.90198428742666
3	3.993041443446152	4.51546414689771
4	1.9860573284590906	2.9945329601857775
5	1.192821443608307	2.2481369730102383
6	0.7871683256739808	1.7803122769929212
7	0.5483143864758717	1.4467883624537368
8	0.41522379739143966	1.2521303683684468
9	0.3315857962183958	1.1249047591796335
>10	3.2379625000813963	27.597525366937166
>50	0.36386056188169436	8.594358974763466
>100	0.06442143487087557	4.791069370792403
>500	0.002593178262993566	0.6497696887498343
>1k	0.0030026274624136025	1.9432383755707634
>5k	2.729661329466912E-4	0.5717253009562081
>10k+	1.364830664733456E-4	3.717166217042104
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72253	3.648559118847699	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5964	0.30116405664550505	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5149	0.2600090086632638	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	4405	0.2224392470696596	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	3689	0.18628340123495443	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	2210	0.11159835097024919	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2099	0.10599318492604212	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1985	0.10023652790766727	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.019879655570121E-4	0.0	0.0	0.19719075137503309	0.0
2	2.019879655570121E-4	0.0	0.0	0.9990324776449819	0.0
3	2.019879655570121E-4	0.0	0.0	1.5813132853544585	0.0
4	2.019879655570121E-4	0.0	0.0	2.108653366432428	0.0
5	2.019879655570121E-4	0.0	0.0	3.511207302268931	0.0
6	2.019879655570121E-4	0.0	0.0	4.4353527416836505	0.0
7	2.019879655570121E-4	0.0	0.0	5.27163341608107	0.0
8	2.019879655570121E-4	0.0	0.0	6.479016480198109	0.0
9	2.019879655570121E-4	0.0	0.0	6.947679057281767	0.0
10	2.019879655570121E-4	0.0	0.0	8.130470086592242	0.0
11	2.019879655570121E-4	0.0	0.0	9.866354662589202	0.0
12	2.5248495694626515E-4	0.0	0.0	11.158269690291853	0.0
13	2.5248495694626515E-4	0.0	0.0	11.688538596870398	0.0
14	2.5248495694626515E-4	0.0	0.0	11.891990975177698	0.0
15	2.5248495694626515E-4	0.0	0.0	12.263547837819823	0.0
16	2.5248495694626515E-4	0.0	0.0	13.104524732416442	0.0
17	2.5248495694626515E-4	0.0	0.0	14.141833929534478	0.0
18	2.5248495694626515E-4	0.0	0.0	15.257716445254191	0.0
19	2.5248495694626515E-4	0.0	0.0	15.934376129870182	0.0
20	2.5248495694626515E-4	0.0	0.0	16.55584260289772	0.0
21	2.5248495694626515E-4	0.0	0.0	17.400606771848533	0.0
22	3.0298194833551815E-4	0.0	0.0	18.25935860741417	0.0
23	3.0298194833551815E-4	0.0	0.0	19.149418577641143	0.0
24	3.0298194833551815E-4	0.0	0.0	19.80209219134724	0.0
25	4.039759311140242E-4	0.0	0.0	20.403410364810465	0.0
26	4.039759311140242E-4	0.0	0.0	20.96826971049065	0.0
27	4.5447292250327725E-4	0.0	0.0	21.546460261897597	0.0
28	4.5447292250327725E-4	0.0	0.0	22.116571294682263	0.0
29	4.5447292250327725E-4	0.0	0.0	22.697690671589786	0.0
30	4.5447292250327725E-4	0.0	0.0	23.3408708509147	0.0
31	5.049699138925303E-4	0.0	0.0	23.938351253032344	0.0
32	5.049699138925303E-4	0.0	0.0	24.527651142544926	0.0
33	5.049699138925303E-4	0.0	0.0	25.07382660141109	0.0
34	5.049699138925303E-4	0.0	0.0	25.597278414152086	0.0
35	5.049699138925303E-4	0.0	0.0	26.121033208841418	0.0
36	5.049699138925303E-4	0.0	0.0	26.656402311550277	0.0
37	5.049699138925303E-4	0.0	0.0	27.17490541913513	0.0
38	5.554669052817834E-4	0.0	0.0	27.709719054938706	0.0
39	5.554669052817834E-4	0.0	0.0	28.22807067154939	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATG	25	3.8918304E-5	45.000004	1
ATAGCGT	20	7.0345245E-4	45.0	13
AATGCGC	20	7.0345245E-4	45.0	44
CGTTTTT	53260	0.0	44.573322	1
TATAGCG	65	0.0	41.53846	1
GTTTTTT	60155	0.0	39.542847	2
TTGCGAG	100	0.0	38.250004	1
ATAGCGG	225	0.0	38.000004	2
TAGTGCG	125	0.0	37.800003	1
TACCCGT	30	1.14022E-4	37.500004	32
CTGCGAA	30	1.14022E-4	37.500004	44
AATAGCG	60	1.5643309E-10	37.500004	1
CGAGATA	205	0.0	37.317074	19
AACGTAG	110	0.0	36.81818	1
TTAGCCG	55	2.752131E-9	36.81818	1
TCGATAG	110	0.0	36.81818	1
TCATCGA	55	2.752131E-9	36.81818	16
TATCGAG	55	2.752131E-9	36.81818	1
TTCGTAG	80	0.0	36.5625	1
CCCTACG	25	0.0021074754	36.000004	32
>>END_MODULE