FastQCFastQC Report
Sat 14 Jan 2017
SRR2934898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503213
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591083.9321107521023304No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC69220.4604803178258836Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC65540.435999422570188Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT38410.25551934423132316Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG33850.22518432184926554No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC30380.20210043420326992Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC21340.14196258281427848Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC19110.12712769248270206No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16160.10750306177501126No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA15720.10457599821183025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG302.165949E-645.0000041
TCCGTTA302.165949E-645.00000420
ACGTAAG302.165949E-645.0000041
CCGTTAG302.165949E-645.00000421
TACCGGT302.165949E-645.00000421
TAGCGTC207.03398E-445.035
CGCATAG253.8913808E-545.01
CCGTCCA207.03398E-445.021
TCGCTAA453.8562575E-1045.014
ACGTGCG502.1827873E-1145.01
TGTTACG502.1827873E-1145.01
CGAATCT207.03398E-445.025
ACGTAAA207.03398E-445.033
CGATCGC207.03398E-445.010
CAATACG502.1827873E-1145.01
CGAAGCA253.8913808E-545.023
CGTTTTT386050.044.5512241
GCTACGA3100.044.27419710
TACGAAT3300.041.59090812
CGTAAGG1250.041.3999982
Produced by FastQC (version 0.11.5)