##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934898.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1503213
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39397610318697	34.0	31.0	34.0	30.0	34.0
2	32.575697522573314	34.0	31.0	34.0	31.0	34.0
3	32.64034571281648	34.0	31.0	34.0	31.0	34.0
4	36.0787918944288	37.0	35.0	37.0	35.0	37.0
5	35.95030045642234	37.0	35.0	37.0	35.0	37.0
6	35.93801144614901	37.0	35.0	37.0	35.0	37.0
7	36.14067800105507	37.0	36.0	37.0	35.0	37.0
8	36.156190107456496	37.0	37.0	37.0	35.0	37.0
9	37.939321307093536	39.0	39.0	39.0	35.0	39.0
10	37.40118067100271	39.0	37.0	39.0	34.0	39.0
11	37.29946920363248	39.0	37.0	39.0	34.0	39.0
12	37.33904509873185	39.0	37.0	39.0	34.0	39.0
13	37.33400123601911	39.0	37.0	39.0	34.0	39.0
14	38.57226221433689	40.0	38.0	41.0	34.0	41.0
15	38.61240090393045	40.0	38.0	41.0	34.0	41.0
16	38.70227439491276	40.0	38.0	41.0	35.0	41.0
17	38.71535770379846	40.0	38.0	41.0	34.0	41.0
18	38.245504130153215	39.0	38.0	40.0	34.0	41.0
19	37.75112309433194	38.0	37.0	40.0	34.0	41.0
20	37.204732795684976	38.0	35.0	40.0	34.0	41.0
21	37.17331010309251	38.0	35.0	40.0	34.0	41.0
22	37.00036388722024	38.0	35.0	40.0	34.0	41.0
23	36.86916558065956	38.0	35.0	40.0	33.0	41.0
24	36.653486897731725	37.0	35.0	40.0	33.0	41.0
25	36.522460888776244	37.0	35.0	40.0	33.0	41.0
26	36.69043841425001	38.0	35.0	40.0	33.0	41.0
27	36.742337912192085	38.0	35.0	40.0	33.0	41.0
28	36.70615275413398	38.0	35.0	40.0	33.0	41.0
29	36.62632241738197	38.0	35.0	40.0	33.0	41.0
30	36.30633050672127	38.0	35.0	40.0	32.0	41.0
31	35.85919826398521	38.0	35.0	40.0	31.0	41.0
32	35.16975305562153	38.0	35.0	40.0	24.0	41.0
33	34.19498833498646	38.0	34.0	40.0	18.0	41.0
34	33.41530907462881	38.0	33.0	40.0	12.0	41.0
35	32.893229369357506	38.0	33.0	40.0	10.0	41.0
36	32.62830882915462	38.0	32.0	40.0	8.0	41.0
37	32.43198668452175	38.0	31.0	40.0	8.0	41.0
38	32.32955076891964	38.0	31.0	40.0	8.0	41.0
39	32.31032461800157	38.0	31.0	40.0	8.0	41.0
40	32.21559153626266	38.0	31.0	40.0	8.0	41.0
41	32.07732902788893	38.0	30.0	40.0	7.0	41.0
42	31.950984990151095	38.0	30.0	40.0	7.0	41.0
43	31.856225963985143	37.0	30.0	40.0	7.0	41.0
44	31.764953469667972	37.0	29.0	40.0	7.0	41.0
45	31.72288025715584	37.0	29.0	40.0	7.0	41.0
46	31.625478225640677	37.0	28.0	40.0	7.0	41.0
47	31.59335170730961	37.0	28.0	40.0	7.0	41.0
48	31.534629490298446	37.0	28.0	40.0	7.0	41.0
49	31.42624431800417	37.0	27.0	40.0	7.0	41.0
50	31.31977836806893	36.0	27.0	40.0	7.0	41.0
51	30.713262192383915	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	11.0
11	8.0
12	12.0
13	15.0
14	32.0
15	56.0
16	113.0
17	239.0
18	481.0
19	927.0
20	1636.0
21	2695.0
22	4440.0
23	8037.0
24	16042.0
25	35517.0
26	61868.0
27	73707.0
28	64348.0
29	50853.0
30	41465.0
31	37354.0
32	38268.0
33	45971.0
34	75812.0
35	97010.0
36	108694.0
37	148593.0
38	278911.0
39	309902.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.122685873525572	17.13782411408097	19.675987368390242	34.063502644003215
2	49.36339693709408	18.69748332405321	18.519065495042952	13.420054243809759
3	22.287726356810378	17.299943520978065	47.13749814563871	13.274831976572848
4	20.58876553089948	19.33165825468513	45.74501417962723	14.334562034788151
5	18.406639644548044	21.648761685802345	45.304091968337154	14.640506701312455
6	19.124768080105746	24.388094035908416	44.521235513530016	11.965902370455817
7	58.70392286389221	4.1502435117312055	32.67161739553875	4.47421622883783
8	58.735122700508846	3.6926902574685028	32.447231363752174	5.124955678270478
9	53.89928107327437	5.521373218565832	34.55298750077334	6.026358207386445
10	28.54518953734434	17.65731137237371	41.50682571265683	12.290673377625128
11	20.8170764888276	19.143860517438313	44.09807525613469	15.940987737599396
12	18.894394872849023	16.61906862167903	48.7491792580293	15.737357247442644
13	17.14866755409912	19.56582333973961	49.13049581130552	14.155013294855753
14	14.708228308296961	21.33875904479272	47.568242158629545	16.38477048828077
15	14.00679743988377	20.14591411862457	50.71749645592474	15.129791985566914
16	15.208090935882007	19.869905329450983	48.17607351719284	16.745930217474168
17	15.432343919324806	20.41280909624917	45.465479609343454	18.689367375082576
18	16.146015235365844	19.681908019688493	48.534771852026296	15.637304892919367
19	15.840403189700995	21.92523614417917	45.79923137971797	16.43512928640186
20	16.526400450235595	21.458036884992346	46.77879981080525	15.236762853966804
21	16.037314738496807	21.925901385898072	47.14441665951532	14.892367216089802
22	14.832761558075935	20.30703566294331	45.69385709144346	19.166345687537294
23	14.364431387966976	20.33790287870049	46.48842180050332	18.809243932829215
24	16.223781992305813	18.93124926407635	47.329486905714624	17.51548183790321
25	14.118092379456538	21.26478416565051	45.265774045328236	19.351349409564715
26	14.346336813212766	21.517110349631093	46.918300999259586	17.21825183789656
27	15.75631663643143	21.768704767720877	45.903474757070356	16.57150383877734
28	12.9922372943821	21.23118945884582	47.9143674249757	17.86220582179638
29	15.477314259522768	19.141133026390804	47.66091033007299	17.720642384013445
30	15.805877144489836	22.424766150904762	45.47592390433026	16.29343280027514
31	15.422232245197453	22.74800710212059	44.869223456689106	16.96053719599285
32	16.912506743887924	22.759648832201425	43.31195911690492	17.015885307005725
33	16.205620893379713	24.072702936975666	41.382691607909194	18.33898456173543
34	15.73822206167722	23.009580145993947	42.16215532994991	19.090042462378918
35	18.167485246601778	23.969124801342193	38.035128754208486	19.828261197847542
36	17.41389942742645	27.07833154715932	36.57718500305678	18.930584022357444
37	16.541701009770403	25.87025258562825	40.163569633844304	17.42447677075704
38	16.57010683116764	26.93078093390624	37.45969466735586	19.039417567570265
39	17.52399693190519	24.784511576203773	38.0713179037169	19.62017358817413
40	19.333321358982396	23.771614534999365	38.14396229942131	18.751101806596935
41	18.586654053683677	24.95168682016454	34.90789395780904	21.553765168342743
42	18.502035307038987	24.025803395792877	35.674851135534354	21.79731016163378
43	18.317497254214803	23.92940987072358	36.89829718077212	20.8547956942895
44	16.675348071098373	24.125589653628595	36.88179918614328	22.317263089129753
45	17.989266990107193	23.066591361304088	36.7202784967932	22.22386315179552
46	19.076538055485152	24.178875515312868	36.93308932267084	19.811497106531142
47	16.538707422035333	23.144491166587837	40.11287821486376	20.203923196513067
48	16.46812527565954	21.767241235939284	39.238284927019656	22.52634856138152
49	17.33932583073723	20.7989153899015	40.265950334383746	21.595808444977525
50	16.7792588275913	20.83809812714499	39.703954130253	22.678688915010714
51	16.52021370224978	20.656154517024532	36.890846473520384	25.932785307205297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	457.0
1	797.0
2	1137.0
3	55324.5
4	109512.0
5	75307.0
6	41102.0
7	38810.0
8	36518.0
9	35988.0
10	35458.0
11	34569.5
12	33681.0
13	31971.5
14	30262.0
15	27977.0
16	25692.0
17	23679.0
18	21666.0
19	19928.5
20	18191.0
21	16665.0
22	15139.0
23	14132.0
24	13125.0
25	13345.0
26	13844.0
27	14123.0
28	14887.5
29	15652.0
30	16389.5
31	17127.0
32	20051.5
33	22976.0
34	25465.5
35	27955.0
36	31599.5
37	35244.0
38	39498.5
39	43753.0
40	52969.0
41	62185.0
42	73155.0
43	84125.0
44	104024.5
45	123924.0
46	141460.5
47	158997.0
48	154922.5
49	150848.0
50	122209.5
51	93571.0
52	78050.0
53	62529.0
54	53659.0
55	44789.0
56	40281.0
57	35773.0
58	32552.5
59	29332.0
60	28308.0
61	27284.0
62	24063.0
63	20842.0
64	17067.0
65	13292.0
66	10830.0
67	8368.0
68	6882.5
69	5397.0
70	4732.5
71	4068.0
72	3323.0
73	2578.0
74	2130.0
75	1208.0
76	734.0
77	511.5
78	289.0
79	230.5
80	172.0
81	110.0
82	48.0
83	35.0
84	22.0
85	20.5
86	19.0
87	9.5
88	0.0
89	2.0
90	4.0
91	2.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1503213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.95338783326586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.93101241380033	28.818391627134883
2	10.767666266513768	8.173388277443415
3	4.375212256364371	4.9816238285596555
4	2.163797104088705	3.2849372273590456
5	1.2912413501213311	2.450349187375235
6	0.8260140290546061	1.8810018480256794
7	0.6122286428270616	1.6265305786694626
8	0.4716757881595108	1.4321355295663407
9	0.3347159094511597	1.1433242452827743
>10	2.744526614611253	22.95957065438557
>50	0.3944272431790189	9.621514488330357
>100	0.07946465185559759	5.675737865778189
>500	0.004810637983958424	1.2161857274867025
>1k	0.0026725766577546796	1.8270172913670126
>5k	3.5634355436729064E-4	0.9112771122385767
>10k+	1.7817177718364532E-4	3.9970145109971646
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59108	3.9321107521023304	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	6922	0.4604803178258836	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	6554	0.435999422570188	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	3841	0.25551934423132316	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	3385	0.22518432184926554	No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3038	0.20210043420326992	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2134	0.14196258281427848	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	1911	0.12712769248270206	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1616	0.10750306177501126	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1572	0.10457599821183025	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.995725156714318E-4	0.0	0.0	0.2690237511250901	0.0
2	1.995725156714318E-4	0.0	0.0	1.5842066293998256	0.0
3	1.995725156714318E-4	0.0	0.0	2.3806340152726193	0.0
4	1.995725156714318E-4	0.0	0.0	3.1151939212872692	0.0
5	1.995725156714318E-4	0.0	0.0	5.018916148277057	0.0
6	1.995725156714318E-4	0.0	0.0	6.083702043556037	0.0
7	1.995725156714318E-4	0.0	0.0	7.13524962862881	0.0
8	1.995725156714318E-4	0.0	0.0	8.719456258028636	0.0
9	1.995725156714318E-4	0.0	0.0	9.316045031542435	0.0
10	1.995725156714318E-4	0.0	0.0	10.700080427723815	0.0
11	1.995725156714318E-4	0.0	0.0	12.860386385695174	0.0
12	1.995725156714318E-4	0.0	0.0	14.382193341861733	0.0
13	1.995725156714318E-4	0.0	0.0	14.986232822627265	0.0
14	1.995725156714318E-4	0.0	0.0	15.220730528541198	0.0
15	1.995725156714318E-4	0.0	0.0	15.674425380834252	0.0
16	1.995725156714318E-4	0.0	0.0	16.762960405478132	0.0
17	1.995725156714318E-4	0.0	0.0	18.08925282045858	0.0
18	1.995725156714318E-4	0.0	0.0	19.48619390598671	0.0
19	1.995725156714318E-4	0.0	0.0	20.30996272650649	0.0
20	1.995725156714318E-4	0.0	0.0	21.06414726322883	0.0
21	1.995725156714318E-4	0.0	0.0	22.083630197450393	0.0
22	1.995725156714318E-4	0.0	0.0	23.123336479926664	0.0
23	1.995725156714318E-4	0.0	0.0	24.220453122744416	0.0
24	1.995725156714318E-4	0.0	0.0	25.038168243622163	0.0
25	2.6609668756190905E-4	0.0	0.0	25.78902657174998	0.0
26	2.6609668756190905E-4	0.0	0.0	26.464446488953993	0.0
27	2.6609668756190905E-4	0.0	0.0	27.103876829165262	0.0
28	2.6609668756190905E-4	0.0	0.0	27.78674745362101	0.0
29	2.6609668756190905E-4	0.0	0.0	28.451922648353893	0.0
30	2.6609668756190905E-4	0.0	0.0	29.17510692097527	0.0
31	3.326208594523863E-4	0.0	0.0	29.897759000221527	0.0
32	3.991450313428636E-4	0.0	0.0	30.596063232555867	0.0
33	3.991450313428636E-4	0.0	0.0	31.227377623796496	0.0
34	3.991450313428636E-4	0.0	0.0	31.811592901338667	0.0
35	4.6566920323334084E-4	0.0	0.0	32.40552070797685	0.0
36	4.6566920323334084E-4	0.0	0.0	33.02792086018415	0.0
37	4.6566920323334084E-4	0.0	0.0	33.64712785214071	0.0
38	4.6566920323334084E-4	0.0	0.0	34.24637759253013	0.0
39	4.6566920323334084E-4	0.0	0.0	34.81349615789645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	30	2.165949E-6	45.000004	1
TCCGTTA	30	2.165949E-6	45.000004	20
ACGTAAG	30	2.165949E-6	45.000004	1
CCGTTAG	30	2.165949E-6	45.000004	21
TACCGGT	30	2.165949E-6	45.000004	21
TAGCGTC	20	7.03398E-4	45.0	35
CGCATAG	25	3.8913808E-5	45.0	1
CCGTCCA	20	7.03398E-4	45.0	21
TCGCTAA	45	3.8562575E-10	45.0	14
ACGTGCG	50	2.1827873E-11	45.0	1
TGTTACG	50	2.1827873E-11	45.0	1
CGAATCT	20	7.03398E-4	45.0	25
ACGTAAA	20	7.03398E-4	45.0	33
CGATCGC	20	7.03398E-4	45.0	10
CAATACG	50	2.1827873E-11	45.0	1
CGAAGCA	25	3.8913808E-5	45.0	23
CGTTTTT	38605	0.0	44.551224	1
GCTACGA	310	0.0	44.274197	10
TACGAAT	330	0.0	41.590908	12
CGTAAGG	125	0.0	41.399998	2
>>END_MODULE