FastQCFastQC Report
Sat 14 Jan 2017
SRR2934895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289087
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT972544.24859343484979No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC55360.24184314532387802No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC41330.1805523337470354No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT28420.1241543025669186Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG27200.11882466677762794No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA24790.10829645181681606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG502.1827873E-1145.01
GCGTACA207.0347544E-445.039
CGTTTTT633950.044.538611
GTTGCGT451.9297659E-840.013
GTTTTTT714700.039.6512532
CGAAACG403.4602272E-739.3751
GCGTAAG900.037.51
TATAGCG601.5643309E-1037.4999961
TCGATGG1750.037.2857132
ACGGGTC1450.037.2413835
ACGTTAG1350.036.6666681
CGCATCG504.8825314E-836.021
ATAGCGT250.002107544336.028
TCGTCAA250.002107544336.043
TGTTACG504.8825314E-836.01
GCGATTC2000.036.09
ATACCGG1900.035.5263142
GCGATAC702.1827873E-1135.3571439
TAGTACG458.7049375E-735.01
TTATGCG1300.034.6153871
Produced by FastQC (version 0.11.5)