##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934895.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289087
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67468427368641	34.0	31.0	34.0	31.0	34.0
2	32.8324244556891	34.0	31.0	34.0	31.0	34.0
3	32.87452901527989	34.0	31.0	34.0	31.0	34.0
4	36.252821321339034	37.0	37.0	37.0	35.0	37.0
5	36.15030315579967	37.0	37.0	37.0	35.0	37.0
6	36.1412764128231	37.0	36.0	37.0	35.0	37.0
7	36.297684185878474	37.0	37.0	37.0	35.0	37.0
8	36.32213367163415	37.0	37.0	37.0	35.0	37.0
9	38.111731445768555	39.0	39.0	39.0	37.0	39.0
10	37.657858788241775	39.0	38.0	39.0	35.0	39.0
11	37.54692110872151	39.0	37.0	39.0	35.0	39.0
12	37.558427879761666	39.0	37.0	39.0	35.0	39.0
13	37.598296613453314	39.0	37.0	39.0	35.0	39.0
14	38.952029346197854	40.0	38.0	41.0	35.0	41.0
15	38.98457245181157	40.0	38.0	41.0	35.0	41.0
16	39.05216970783548	41.0	38.0	41.0	35.0	41.0
17	39.05962289768803	41.0	38.0	41.0	35.0	41.0
18	38.53642172621661	39.0	38.0	41.0	35.0	41.0
19	38.0298415918661	39.0	37.0	41.0	35.0	41.0
20	37.45648723705128	38.0	35.0	41.0	35.0	41.0
21	37.39252068619498	38.0	35.0	41.0	34.0	41.0
22	37.19578810241813	38.0	35.0	41.0	34.0	41.0
23	37.101021498964435	38.0	35.0	40.0	34.0	41.0
24	36.9146760258566	38.0	35.0	40.0	33.0	41.0
25	36.75885014418412	38.0	35.0	40.0	33.0	41.0
26	36.90674578991537	38.0	35.0	40.0	34.0	41.0
27	36.9479840652627	38.0	35.0	40.0	34.0	41.0
28	36.88249157852017	38.0	35.0	40.0	33.0	41.0
29	36.75890824595133	38.0	35.0	40.0	33.0	41.0
30	36.451192549693396	38.0	35.0	41.0	33.0	41.0
31	35.88971978784555	38.0	35.0	41.0	31.0	41.0
32	35.13982954776293	38.0	35.0	41.0	23.0	41.0
33	34.14911403542111	38.0	34.0	41.0	16.0	41.0
34	33.28490136023664	38.0	33.0	41.0	12.0	41.0
35	32.79306596909598	38.0	33.0	41.0	10.0	41.0
36	32.54535367157299	38.0	32.0	41.0	8.0	41.0
37	32.28817078599459	38.0	31.0	41.0	8.0	41.0
38	32.17350105085565	38.0	31.0	41.0	7.0	41.0
39	32.07950680773601	38.0	30.0	41.0	7.0	41.0
40	31.93252156864287	37.0	30.0	40.0	7.0	41.0
41	31.80694005950844	37.0	29.0	40.0	7.0	41.0
42	31.70537948098958	37.0	29.0	40.0	7.0	41.0
43	31.638491241267808	37.0	28.0	40.0	7.0	41.0
44	31.53877593992714	37.0	27.0	40.0	7.0	41.0
45	31.48508029620543	37.0	27.0	40.0	7.0	41.0
46	31.423352629236025	37.0	26.0	40.0	7.0	41.0
47	31.398682968362497	36.0	26.0	40.0	7.0	41.0
48	31.350202941172615	36.0	26.0	40.0	7.0	41.0
49	31.22613469911803	36.0	26.0	40.0	7.0	41.0
50	31.147086589544216	36.0	26.0	40.0	7.0	41.0
51	30.58323951863778	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	20.0
10	23.0
11	16.0
12	24.0
13	29.0
14	44.0
15	100.0
16	177.0
17	335.0
18	665.0
19	1295.0
20	2316.0
21	3761.0
22	6254.0
23	11011.0
24	22531.0
25	49589.0
26	92151.0
27	118426.0
28	109855.0
29	85710.0
30	65885.0
31	55334.0
32	52101.0
33	61096.0
34	103125.0
35	134523.0
36	161166.0
37	204626.0
38	394773.0
39	551754.0
40	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.546178891409546	16.04565488336616	18.099049970577788	38.309116254646504
2	51.02942788980934	17.850304510051387	18.7469065177514	12.37336108238787
3	22.856973107618888	17.645026161085184	47.21013224923299	12.287868482062937
4	20.778720948570324	18.85065967348554	47.13119247979653	13.239426898147602
5	19.00277272117661	21.214047347261157	45.76208767949842	14.021092252063813
6	19.600259841587498	24.39771839165571	44.72180393318384	11.28021783357295
7	59.95088871676787	4.053362759912576	32.61898739541136	3.376761127908201
8	61.253809925092405	3.302058855779619	32.192660217807365	3.251471001320614
9	56.72300790664575	5.315743787807104	33.724144167521814	4.237104138025335
10	28.598738274255194	21.11112421677289	39.447299294434856	10.842838214537062
11	20.703581821049177	19.20757052921099	46.17260069189157	13.91624695784826
12	18.959960892705258	17.03146276222791	49.053050408306895	14.955525936759939
13	17.154918096166725	17.324898529413694	50.211721966006536	15.308461408413049
14	14.793365215039882	19.504239026301754	49.12163670493957	16.580759053718797
15	14.351442299921322	19.47807138828712	51.23291513166603	14.937571180125525
16	16.071429351527485	19.303460287878966	49.37112481963333	15.253985540960215
17	16.214848976906513	19.18489773433688	47.86445425621656	16.735799032540047
18	16.276926128189974	19.179087557615766	48.98446411167422	15.559522202520043
19	17.068901269370716	19.614326585228085	47.749954457825325	15.566817687575876
20	18.01202837637888	19.77067713022703	47.727500090647496	14.489794402746597
21	17.57368767547935	19.66080799899698	48.13600356823485	14.629500757288824
22	17.076589924279855	18.728995446656242	47.35058125794257	16.843833371121324
23	16.296147765462823	18.741271083187314	48.2694628906634	16.693118260686465
24	16.495790679864943	18.270996253091297	48.67307358785402	16.56013947918974
25	15.474379086509163	20.179573777667688	47.59469605130779	16.751351084515353
26	15.170327733284056	20.251785974058652	47.79822697870373	16.779659313953555
27	15.670745585467044	19.568631511165805	48.12158734028021	16.639035563086942
28	14.601323584468393	19.78308382337587	48.661846404265106	16.95374618789063
29	15.915122492067798	18.66088969095539	48.54468178797923	16.879306028997586
30	16.88013605424346	19.16205019730574	47.08803116701113	16.869782581439672
31	17.380947076279757	20.5409405583973	45.39311961493818	16.68499275038476
32	18.17266010422496	21.445274906545713	43.60511417870968	16.776950810519654
33	18.260468038130487	22.796687063444946	41.38933994208171	17.553504956342856
34	18.03055104502363	23.043772473479603	40.872671069295315	18.053005412201458
35	18.552331125903034	23.900227470602907	38.248305984001476	19.299135419492575
36	18.991938707441	25.81142612753469	37.318590337545054	17.878044827479254
37	19.545521860899125	25.96480605586419	37.152279489595635	17.337392593641045
38	20.095697542295245	26.588897669682282	35.12387253083871	18.19153225718376
39	20.244665231159846	25.47723175222261	36.19862416762666	18.079478848990885
40	19.520490046905163	25.118092933995083	37.1341499908042	18.22726702829556
41	18.138803811301187	26.41350022956751	35.50983427016972	19.93786168896158
42	18.753153549865075	26.50978315808879	35.18961926741972	19.54744402462641
43	19.53267831235772	25.944317537952905	35.01522659470785	19.50777755498153
44	19.27126404544694	25.389729617091877	35.30595385845972	20.033052479001455
45	19.281617518250727	24.18322239390639	35.81419142216962	20.720968665673254
46	19.764517469191865	24.591376387179693	36.415042329103265	19.22906381452518
47	18.23146084006418	24.509553372152304	38.04564876739067	19.213337020392846
48	18.44787900154079	23.308550526913134	38.26171744455322	19.98185302699286
49	18.705099456682948	23.230702895958082	38.62483164685309	19.439366000505878
50	18.219884172161215	22.608664502485052	38.911627212071885	20.259824113281848
51	18.07039225682554	22.577647769612952	37.712371788402976	21.639588185158537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	397.0
1	961.5
2	1526.0
3	98447.0
4	195368.0
5	134190.0
6	73012.0
7	68506.5
8	64001.0
9	62008.5
10	60016.0
11	57263.0
12	54510.0
13	51063.5
14	47617.0
15	43342.0
16	39067.0
17	35586.5
18	32106.0
19	29468.0
20	26830.0
21	24723.5
22	22617.0
23	21264.0
24	19911.0
25	20112.0
26	21893.0
27	23473.0
28	25757.5
29	28042.0
30	30781.5
31	33521.0
32	36989.0
33	40457.0
34	45729.0
35	51001.0
36	56075.5
37	61150.0
38	65301.5
39	69453.0
40	73347.0
41	77241.0
42	83561.0
43	89881.0
44	96929.5
45	103978.0
46	110103.5
47	116229.0
48	128368.5
49	140508.0
50	150078.0
51	159648.0
52	158018.5
53	156389.0
54	133507.0
55	110625.0
56	100644.5
57	90664.0
58	81819.0
59	72974.0
60	68751.5
61	64529.0
62	57465.0
63	50401.0
64	42761.0
65	35121.0
66	28374.5
67	21628.0
68	17830.0
69	14032.0
70	11402.0
71	8772.0
72	7013.5
73	5255.0
74	4353.5
75	2630.5
76	1809.0
77	1322.5
78	836.0
79	600.0
80	364.0
81	261.5
82	159.0
83	120.5
84	82.0
85	90.0
86	98.0
87	55.5
88	13.0
89	9.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2289087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.787114659604416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41137108254426	24.606841285580202
2	9.667394969852745	6.145971847327844
3	3.6868484945386637	3.515828274854713
4	1.7883037016594903	2.273800592433809
5	0.9912056127807563	1.5753783232352676
6	0.6767989660962966	1.2908091802082822
7	0.47985901509265527	1.0677333473236554
8	0.3601277368885926	0.9157937331665237
9	0.2908909781210755	0.8321926387481196
>10	3.76641061564165	30.265404737092684
>50	0.802831582419649	16.20050488170169
>100	0.07108712275481818	4.486260099571168
>500	0.004346403467134404	0.9296805758546562
>1k	0.0022433050152951763	1.312694711468027
>5k	1.4020656345594852E-4	0.2467263503322808
>10k+	1.4020656345594852E-4	4.33437942110109
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97254	4.24859343484979	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	5536	0.24184314532387802	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	4133	0.1805523337470354	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	2842	0.1241543025669186	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	2720	0.11882466677762794	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2479	0.10829645181681606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.184276962824043E-4	0.0	0.0	0.13179927193680274	0.0
2	2.184276962824043E-4	0.0	0.0	0.7118995477236121	0.0
3	2.184276962824043E-4	0.0	0.0	1.0577142764779146	0.0
4	2.184276962824043E-4	0.0	0.0	1.3694979701514185	0.0
5	2.184276962824043E-4	0.0	0.0	2.1881649758178696	0.0
6	2.184276962824043E-4	0.0	0.0	2.6592261456205026	0.0
7	2.184276962824043E-4	0.0	0.0	3.1452714553881087	0.0
8	2.184276962824043E-4	0.0	0.0	3.903259247027308	0.0
9	2.184276962824043E-4	0.0	0.0	4.1939865107791885	0.0
10	2.184276962824043E-4	0.0	0.0	4.835246541525071	0.0
11	2.184276962824043E-4	0.0	0.0	5.795716807618059	0.0
12	2.184276962824043E-4	0.0	0.0	6.56187379509822	0.0
13	2.184276962824043E-4	0.0	0.0	6.867017286804739	0.0
14	2.184276962824043E-4	0.0	0.0	6.992831639863404	0.0
15	2.184276962824043E-4	0.0	0.0	7.21781216703428	0.0
16	2.184276962824043E-4	0.0	0.0	7.711764559407309	0.0
17	2.184276962824043E-4	0.0	0.0	8.310431189378123	0.0
18	2.184276962824043E-4	0.0	0.0	8.979256795394845	0.0
19	2.184276962824043E-4	0.0	0.0	9.393483078624797	0.0
20	2.184276962824043E-4	0.0	0.0	9.806966707687389	0.0
21	2.621132355388852E-4	0.0	0.0	10.325994599593637	0.0
22	2.621132355388852E-4	0.0	0.0	10.87625765206827	0.0
23	3.05798774795366E-4	0.0	0.0	11.42306954694164	0.0
24	3.05798774795366E-4	0.0	0.0	11.85830857455396	0.0
25	3.05798774795366E-4	0.0	0.0	12.251128943548236	0.0
26	3.05798774795366E-4	0.0	0.0	12.609000881137327	0.0
27	3.4948431405184687E-4	0.0	0.0	12.960451044455715	0.0
28	3.931698533083277E-4	0.0	0.0	13.329899649947773	0.0
29	3.931698533083277E-4	0.0	0.0	13.71887569148748	0.0
30	4.368553925648086E-4	0.0	0.0	14.13869372374226	0.0
31	4.8054093182128946E-4	0.0	0.0	14.535664218965902	0.0
32	4.8054093182128946E-4	0.0	0.0	14.911141428875355	0.0
33	4.8054093182128946E-4	0.0	0.0	15.26722225935493	0.0
34	4.8054093182128946E-4	0.0	0.0	15.63741351901435	0.0
35	4.8054093182128946E-4	0.0	0.0	16.036044064729737	0.0
36	5.242264710777704E-4	0.0	0.0	16.423971653327285	0.0
37	5.242264710777704E-4	0.0	0.0	16.793070774505296	0.0
38	5.242264710777704E-4	0.0	0.0	17.150549542241077	0.0
39	5.242264710777704E-4	0.0	0.0	17.51112998326407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	50	2.1827873E-11	45.0	1
GCGTACA	20	7.0347544E-4	45.0	39
CGTTTTT	63395	0.0	44.53861	1
GTTGCGT	45	1.9297659E-8	40.0	13
GTTTTTT	71470	0.0	39.651253	2
CGAAACG	40	3.4602272E-7	39.375	1
GCGTAAG	90	0.0	37.5	1
TATAGCG	60	1.5643309E-10	37.499996	1
TCGATGG	175	0.0	37.285713	2
ACGGGTC	145	0.0	37.241383	5
ACGTTAG	135	0.0	36.666668	1
CGCATCG	50	4.8825314E-8	36.0	21
ATAGCGT	25	0.0021075443	36.0	28
TCGTCAA	25	0.0021075443	36.0	43
TGTTACG	50	4.8825314E-8	36.0	1
GCGATTC	200	0.0	36.0	9
ATACCGG	190	0.0	35.526314	2
GCGATAC	70	2.1827873E-11	35.357143	9
TAGTACG	45	8.7049375E-7	35.0	1
TTATGCG	130	0.0	34.615387	1
>>END_MODULE