##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934894.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470201 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61498257721223 34.0 31.0 34.0 31.0 34.0 2 32.76812830354489 34.0 31.0 34.0 31.0 34.0 3 32.81682028511748 34.0 31.0 34.0 31.0 34.0 4 36.21638197770237 37.0 37.0 37.0 35.0 37.0 5 36.10358039478955 37.0 35.0 37.0 35.0 37.0 6 36.10129091192293 37.0 36.0 37.0 35.0 37.0 7 36.24287427365374 37.0 37.0 37.0 35.0 37.0 8 36.2379552183681 37.0 37.0 37.0 35.0 37.0 9 38.00804447827202 39.0 39.0 39.0 35.0 39.0 10 37.64842698379337 39.0 38.0 39.0 35.0 39.0 11 37.530962773117416 39.0 37.0 39.0 35.0 39.0 12 37.504922116091606 39.0 37.0 39.0 35.0 39.0 13 37.49137770957849 39.0 37.0 39.0 35.0 39.0 14 38.812707242070985 40.0 38.0 41.0 35.0 41.0 15 38.86580134280959 40.0 38.0 41.0 35.0 41.0 16 38.94741671376907 40.0 38.0 41.0 35.0 41.0 17 38.97286289425732 40.0 38.0 41.0 35.0 41.0 18 38.438905292541634 39.0 38.0 41.0 35.0 41.0 19 37.95037481269568 38.0 37.0 41.0 35.0 41.0 20 37.35502492516329 38.0 35.0 41.0 34.0 41.0 21 37.299773976483486 38.0 35.0 41.0 34.0 41.0 22 37.109805393956336 38.0 35.0 41.0 34.0 41.0 23 36.99672153671505 38.0 35.0 40.0 34.0 41.0 24 36.79057693471845 38.0 35.0 40.0 33.0 41.0 25 36.64077973011854 37.0 35.0 40.0 33.0 41.0 26 36.75356430855373 38.0 35.0 40.0 33.0 41.0 27 36.758937723481345 38.0 35.0 40.0 33.0 41.0 28 36.67742641992489 38.0 35.0 40.0 33.0 41.0 29 36.5510151333049 38.0 35.0 40.0 33.0 41.0 30 36.230711310902386 38.0 35.0 40.0 32.0 41.0 31 35.689300306556724 38.0 35.0 40.0 30.0 41.0 32 34.92025716211593 38.0 35.0 40.0 22.0 41.0 33 33.905454424259 38.0 34.0 40.0 16.0 41.0 34 33.07346410456802 38.0 33.0 40.0 10.0 41.0 35 32.546038262795356 38.0 32.0 40.0 9.0 41.0 36 32.290014766688365 38.0 31.0 40.0 8.0 41.0 37 32.03633652813459 37.0 30.0 40.0 7.0 41.0 38 31.86832276675094 37.0 30.0 40.0 7.0 41.0 39 31.801290435797554 37.0 29.0 40.0 7.0 41.0 40 31.656117768930915 37.0 29.0 40.0 7.0 41.0 41 31.484345337814354 37.0 27.0 40.0 7.0 41.0 42 31.345237828024874 37.0 26.0 40.0 7.0 41.0 43 31.220940538062482 37.0 25.0 40.0 7.0 41.0 44 31.1114711525839 36.0 24.0 40.0 7.0 41.0 45 31.072685299493063 36.0 24.0 40.0 7.0 41.0 46 31.002270437851696 36.0 24.0 40.0 7.0 41.0 47 30.95909266828141 36.0 24.0 40.0 7.0 41.0 48 30.879499469800386 35.0 24.0 40.0 7.0 41.0 49 30.744125463116948 35.0 24.0 40.0 7.0 41.0 50 30.615609022167718 35.0 24.0 40.0 7.0 41.0 51 30.045266599601007 35.0 24.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 9.0 10 20.0 11 16.0 12 15.0 13 23.0 14 42.0 15 64.0 16 134.0 17 303.0 18 549.0 19 1076.0 20 1858.0 21 2886.0 22 4554.0 23 8022.0 24 15581.0 25 34656.0 26 63016.0 27 79321.0 28 72233.0 29 55511.0 30 42254.0 31 36468.0 32 35545.0 33 41450.0 34 69697.0 35 93980.0 36 103459.0 37 134006.0 38 243998.0 39 329197.0 40 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.78470290796973 15.78103946331148 17.166836371353305 37.26742125736549 2 51.845972081368465 17.794437631317077 17.681255828284705 12.678334459029752 3 23.234102003739622 17.40285852070567 46.36502083728688 12.998018638267828 4 21.62602256426162 18.541478342077035 45.774081231069765 14.058417862591577 5 18.100042103086587 21.944006295737793 45.50221364289645 14.453737958279175 6 20.213630653223607 24.444616756484315 44.239801224458425 11.101951365833651 7 58.14749139743477 4.608349470582594 33.30483382884381 3.9393253031388227 8 58.449831009501416 4.546385154138788 33.13009581683049 3.8736880195293026 9 53.41888626113028 6.549852707214863 34.76170945333325 5.269551578321604 10 29.054462621097386 21.20710025363879 39.71817458973297 10.020262535530856 11 22.74491719159489 19.290831661793185 44.60920649625459 13.355044650357332 12 21.557800600054005 16.729821296543808 47.33536434814015 14.377013755262036 13 18.428908700238946 18.1654073150542 48.496838187431514 14.908845797275339 14 14.582495862810596 20.942442563975945 47.601110324370616 16.873951248842843 15 13.987815271517295 20.091674539739802 51.184021776614216 14.736488412128681 16 15.571476281134347 18.960672724341773 50.23394760308284 15.233903391441034 17 15.879733451412426 18.402313697242757 47.389710658610625 18.32824219273419 18 15.713157588656246 19.518351572336027 48.088798742484876 16.679692096522857 19 18.095961028458014 19.9365937038541 46.38399783430973 15.583447433378156 20 19.729819256006493 19.369120276751275 46.65668163740877 14.24437882983347 21 18.433942025614183 19.06188337513034 46.86502049719732 15.639154102058153 22 17.118135547452354 18.95183039597987 46.05363484312689 17.876399213440884 23 17.255599744524726 19.60371404998364 47.02826348234017 16.11242272315146 24 16.983460084709506 18.273623810621814 47.642669267671565 17.10024683699712 25 16.497744185999057 19.518351572336027 46.119340144646884 17.864564097018025 26 16.337766060559066 21.266207817842595 45.9956155654907 16.400410556107634 27 16.87279494436475 21.62690679709781 46.135392371519266 15.364905887018171 28 15.31477668699722 21.39877472536068 46.784827380745895 16.5016212068962 29 16.679556060701902 19.79906148887125 46.1187279834526 17.402654466974244 30 17.47386921924281 20.52909772201216 45.154642120363135 16.842390938381897 31 17.49488675357995 22.092761465949216 42.78755081788137 17.624800962589468 32 18.486927977875133 23.553582129246273 41.22701589782621 16.732473995052377 33 18.834703554139875 24.545963443093836 39.41753542542823 17.201797577338066 34 18.609292198821795 24.98025780148429 38.09445103084544 18.315998968848476 35 19.012570390035105 25.992500345190894 35.02249012209895 19.97243914267505 36 19.756414259002682 28.380201074546953 33.847956843996165 18.015427822454207 37 19.229411488633186 28.785859892626924 34.88516196084753 17.099566657892357 38 18.83021437204845 28.240628322249815 33.537387064761894 19.39177024093984 39 20.293823769675033 25.92019730635471 34.305037202396136 19.480941721574123 40 21.090517555082606 25.927883330238515 35.38971882075988 17.591880293918997 41 18.405782610677043 28.07935785651078 34.093501500815194 19.421358031996984 42 18.96427767359701 28.61601917016789 33.25327625270286 19.166426903532237 43 19.679622038075063 27.712809336954606 33.153085870571445 19.45448275439889 44 19.096708545294145 27.465224142821288 32.79762427042289 20.64044304146168 45 19.403537339452225 25.98243369444042 32.65138576289909 21.96264320320827 46 19.27756816925033 27.198185826291777 33.567450981192366 19.956795023265524 47 18.4076190942599 26.525624727503246 35.86162708364367 19.205129094593186 48 18.179691076254198 25.62853650623282 35.21824566844941 20.973526749063566 49 18.259135995690386 24.597589037145262 36.21634048677698 20.926934480387377 50 17.38884683114758 24.8405490133662 36.16559912556174 21.605005029924477 51 17.26002090870568 24.024266069741486 35.05731529226276 23.658397729290076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 383.0 1 745.0 2 1107.0 3 57553.5 4 114000.0 5 79925.0 6 45850.0 7 44005.0 8 42160.0 9 40876.0 10 39592.0 11 37821.0 12 36050.0 13 33869.0 14 31688.0 15 29308.0 16 26928.0 17 24659.5 18 22391.0 19 20605.5 20 18820.0 21 17100.0 22 15380.0 23 14219.0 24 13058.0 25 12988.5 26 14203.5 27 15488.0 28 16052.5 29 16617.0 30 17897.0 31 19177.0 32 20567.5 33 21958.0 34 24889.5 35 27821.0 36 30837.0 37 33853.0 38 36269.5 39 38686.0 40 43376.0 41 48066.0 42 53533.0 43 59000.0 44 65688.0 45 72376.0 46 82234.0 47 92092.0 48 98774.5 49 105457.0 50 102615.5 51 99774.0 52 87074.5 53 74375.0 54 65774.5 55 57174.0 56 54278.0 57 51382.0 58 50705.0 59 50028.0 60 50441.0 61 50854.0 62 45653.5 63 40453.0 64 33776.5 65 27100.0 66 22157.0 67 17214.0 68 14401.5 69 11589.0 70 9502.0 71 7415.0 72 6438.5 73 5462.0 74 4191.5 75 2412.5 76 1904.0 77 1365.5 78 827.0 79 604.0 80 381.0 81 341.5 82 302.0 83 186.0 84 70.0 85 42.0 86 14.0 87 19.5 88 25.0 89 15.5 90 6.0 91 6.5 92 7.0 93 6.5 94 6.0 95 3.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1470201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.828223670210306 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42608564746114 26.530151668613854 2 9.414884798999218 6.369776576196171 3 3.4799409798396206 3.531606654754365 4 1.6952420038847622 2.2938810273019707 5 0.978065830723164 1.654311484294662 6 0.6464082133259748 1.3120104973591253 7 0.4587505748391006 1.0863101938146253 8 0.3601441300612031 0.9746428948178953 9 0.2830833957406662 0.8618587585594126 >10 3.46923045183443 29.236801225409643 >50 0.6976650066388762 15.058397685376484 >100 0.08495821384197168 5.1266623701027 >500 0.002872982938513373 0.6576150472642232 >1k 0.002462556804440034 1.358825813907939 >5k 0.0 0.0 >10k+ 2.052130670366695E-4 3.9471481022270103 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56859 3.8674303717654936 No Hit CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC 3500 0.23806268666665303 No Hit GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC 3416 0.2323491821866534 No Hit CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT 1718 0.11685477019808856 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1497 0.10182281198285131 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.12692142094856418 0.0 2 0.0 0.0 0.0 0.7850627227161456 0.0 3 0.0 0.0 0.0 1.12780497360565 0.0 4 0.0 0.0 0.0 1.4867354871884866 0.0 5 0.0 0.0 0.0 2.470410508495097 0.0 6 0.0 0.0 0.0 2.970614222136973 0.0 7 0.0 0.0 0.0 3.490475111906467 0.0 8 0.0 0.0 0.0 4.271864867456899 0.0 9 0.0 0.0 0.0 4.577877446689262 0.0 10 0.0 0.0 0.0 5.2473777395063665 0.0 11 0.0 0.0 0.0 6.207586581698693 0.0 12 0.0 0.0 0.0 7.005096582031981 0.0 13 0.0 0.0 0.0 7.310565017980535 0.0 14 0.0 0.0 0.0 7.437894546391956 0.0 15 0.0 0.0 0.0 7.692213513662417 0.0 16 0.0 0.0 0.0 8.201531627308103 0.0 17 0.0 0.0 0.0 8.816617591744258 0.0 18 0.0 0.0 0.0 9.506523257704218 0.0 19 0.0 0.0 0.0 9.911433878768957 0.0 20 0.0 0.0 0.0 10.328995831182267 0.0 21 0.0 0.0 0.0 10.820289198551762 0.0 22 0.0 0.0 0.0 11.373206792812683 0.0 23 6.801791047618659E-5 0.0 0.0 11.881572655711702 0.0 24 6.801791047618659E-5 0.0 0.0 12.284578775283107 0.0 25 6.801791047618659E-5 0.0 0.0 12.656092602304039 0.0 26 6.801791047618659E-5 0.0 0.0 12.993393420355448 0.0 27 6.801791047618659E-5 0.0 0.0 13.332394686168762 0.0 28 6.801791047618659E-5 0.0 0.0 13.687040071391598 0.0 29 6.801791047618659E-5 0.0 0.0 14.060458399905864 0.0 30 1.3603582095237317E-4 0.0 0.0 14.473191080675363 0.0 31 1.3603582095237317E-4 0.0 0.0 14.870211624124865 0.0 32 1.3603582095237317E-4 0.0 0.0 15.271653331755317 0.0 33 2.0405373142855977E-4 0.0 0.0 15.635345099071488 0.0 34 2.0405373142855977E-4 0.0 0.0 15.9914868783248 0.0 35 2.0405373142855977E-4 0.0 0.0 16.373951588932396 0.0 36 2.0405373142855977E-4 0.0 0.0 16.772808615964756 0.0 37 2.0405373142855977E-4 0.0 0.0 17.182684544494258 0.0 38 2.0405373142855977E-4 0.0 0.0 17.557259177486614 0.0 39 2.0405373142855977E-4 0.0 0.0 17.932377953762785 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCGA 20 7.03393E-4 45.0 24 TGTTACG 35 1.2122291E-7 45.0 1 ACTCGAG 25 3.8913393E-5 45.0 1 TATAGCG 20 7.03393E-4 45.0 1 ACGTAAG 25 3.8913393E-5 45.0 1 TCGGTAG 20 7.03393E-4 45.0 1 TCGAACG 30 2.165918E-6 44.999996 2 CGTTTTT 41315 0.0 44.504417 1 TCGACGT 125 0.0 41.399998 26 GTTAGCG 120 0.0 41.249996 1 TAACGCC 60 3.6379788E-12 41.249996 12 GTACGAG 50 1.0822987E-9 40.5 1 GTTTTTT 46005 0.0 40.11412 2 AATACGG 85 0.0 39.705883 2 CGGGTAT 80 0.0 39.375 6 TCTAGCG 40 3.4590994E-7 39.375 1 CGAATAT 305 0.0 39.098362 14 TTCGACG 145 0.0 38.793102 25 CGCAGTA 35 6.24948E-6 38.571426 37 TGCTCGC 35 6.24948E-6 38.571426 11 >>END_MODULE