##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934892.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071367
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62892734235794	34.0	31.0	34.0	31.0	34.0
2	32.78409639273937	34.0	31.0	34.0	31.0	34.0
3	32.83313374408583	34.0	31.0	34.0	31.0	34.0
4	36.2239335353805	37.0	37.0	37.0	35.0	37.0
5	36.114065488296724	37.0	35.0	37.0	35.0	37.0
6	36.11275127944019	37.0	36.0	37.0	35.0	37.0
7	36.2441572309022	37.0	37.0	37.0	35.0	37.0
8	36.259949205080986	37.0	37.0	37.0	35.0	37.0
9	38.05103666624042	39.0	39.0	39.0	37.0	39.0
10	37.67741586216488	39.0	38.0	39.0	35.0	39.0
11	37.56205389936408	39.0	37.0	39.0	35.0	39.0
12	37.45523896106563	39.0	37.0	39.0	35.0	39.0
13	37.406448023879776	39.0	37.0	39.0	35.0	39.0
14	38.70143844266251	40.0	38.0	41.0	35.0	41.0
15	38.75614985341158	40.0	38.0	41.0	35.0	41.0
16	38.81802687594447	40.0	38.0	41.0	35.0	41.0
17	38.80572576904086	40.0	38.0	41.0	35.0	41.0
18	38.32130913123141	39.0	38.0	41.0	35.0	41.0
19	37.824586719583486	38.0	37.0	41.0	35.0	41.0
20	37.26482521862256	38.0	35.0	41.0	34.0	41.0
21	37.211329077711	38.0	35.0	41.0	34.0	41.0
22	37.0247160870178	38.0	35.0	40.0	34.0	41.0
23	36.912812322948156	37.0	35.0	40.0	34.0	41.0
24	36.71687666317891	37.0	35.0	40.0	33.0	41.0
25	36.556295835133994	37.0	35.0	40.0	33.0	41.0
26	36.67729825540641	37.0	35.0	40.0	33.0	41.0
27	36.73190699358857	37.0	35.0	40.0	33.0	41.0
28	36.66953714273447	37.0	35.0	40.0	33.0	41.0
29	36.54270105388723	37.0	35.0	40.0	33.0	41.0
30	36.2441805655765	37.0	35.0	40.0	33.0	41.0
31	35.736150170763146	37.0	35.0	40.0	31.0	41.0
32	34.998265767006075	37.0	35.0	40.0	23.0	41.0
33	34.0496953891617	37.0	34.0	40.0	17.0	41.0
34	33.243144506037616	37.0	33.0	40.0	12.0	41.0
35	32.75662681415425	37.0	33.0	40.0	10.0	41.0
36	32.54159405693847	37.0	32.0	40.0	9.0	41.0
37	32.321647017315264	37.0	31.0	40.0	8.0	41.0
38	32.17111503341059	37.0	31.0	40.0	8.0	41.0
39	32.108761050135016	37.0	31.0	40.0	8.0	41.0
40	31.966189923714282	37.0	30.0	40.0	8.0	41.0
41	31.816576392590026	36.0	30.0	40.0	7.0	41.0
42	31.678976485182016	36.0	29.0	40.0	7.0	41.0
43	31.56164134232247	36.0	29.0	40.0	7.0	41.0
44	31.460827148866823	36.0	29.0	40.0	7.0	41.0
45	31.417633733351877	35.0	28.0	40.0	7.0	41.0
46	31.34860136629185	35.0	27.0	40.0	7.0	41.0
47	31.33923576141509	35.0	27.0	40.0	7.0	41.0
48	31.297453627001765	35.0	27.0	40.0	7.0	41.0
49	31.176479208338506	35.0	27.0	40.0	7.0	41.0
50	31.098704738898995	35.0	27.0	40.0	7.0	41.0
51	30.528812255744295	35.0	25.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	7.0
11	11.0
12	6.0
13	20.0
14	26.0
15	53.0
16	83.0
17	171.0
18	376.0
19	667.0
20	1141.0
21	1809.0
22	2942.0
23	4928.0
24	9864.0
25	21349.0
26	40701.0
27	53188.0
28	50488.0
29	40956.0
30	32756.0
31	28339.0
32	27462.0
33	31827.0
34	58899.0
35	81423.0
36	78988.0
37	94117.0
38	173954.0
39	234645.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.40504981019576	15.93636914334677	17.64344057638512	37.015140470072346
2	50.4295913538498	18.14046913895985	18.15036304086275	13.279576466327597
3	22.467277786230117	17.716991469776463	46.65497443919777	13.160756304795648
4	21.869256753288088	18.76415831363109	45.747068931561266	13.619516001519555
5	18.53081157063826	21.872803623781582	44.92503502534612	14.671349780234038
6	19.86518158576846	23.858491067953373	44.46011497460721	11.816212371670959
7	58.58356660229408	4.807222921743903	32.82385961113232	3.7853508648296987
8	59.520500444758895	3.9252655719282	32.413169343464936	4.141064639847969
9	54.89547465994379	5.796799789427899	34.124721033968754	5.183004516659557
10	30.57598376653378	19.874142100699387	38.86053985235685	10.68933428040998
11	24.33246497232041	17.908989169910964	43.766981809221306	13.991564048547323
12	21.90817899001929	16.70940023353342	47.02982264714146	14.35259812930583
13	17.975632999709717	18.7085284501016	47.939501590024705	15.376336960163977
14	14.53843547542532	21.637030074661624	47.5947084425785	16.229826007334555
15	14.614879868429773	18.85656362385625	51.49477256626347	15.033783941450501
16	16.342485814851493	18.800187050749184	49.69641588736633	15.160911247032995
17	15.763785892229274	18.22587404689523	47.21127307449268	18.79906698638282
18	16.72340103811299	19.227491606517656	48.16967481731284	15.87943253805652
19	17.491298499953796	20.02479075797556	46.430308195044276	16.05360254702637
20	19.44506410968417	19.724520169092383	46.30290087337019	14.527514847853256
21	17.914962846531584	20.04980552882439	46.74168608889391	15.293545535750122
22	16.985869454631324	18.992838121764063	46.50815266850669	17.513139755097924
23	16.913345286909156	19.41351563003154	46.84501202669114	16.82812705636817
24	17.90030867107163	17.55243534661792	47.55746630239685	16.989789679913606
25	15.597549672521179	19.56379093251892	46.54791495351267	18.290744441447234
26	14.488872627213645	21.679219165794727	46.573396417847476	17.258511789144148
27	15.963810720322726	20.819943119397927	46.445895757476194	16.770350402803146
28	13.985217017137918	21.141214915150456	47.65425853139027	17.219309536321354
29	16.082444204460284	20.289125948437835	46.546421534357506	17.082008312744374
30	16.287042628716396	20.412239690040852	45.03517468803874	18.265542993204008
31	18.092586387297725	21.567119390460974	42.72765541593124	17.61263880631007
32	18.85955046216656	23.16853141827217	41.180379832494374	16.791538287066896
33	18.046850425671128	23.831422845766205	39.04917735939225	19.072549369170417
34	17.279512996013505	24.959701017485138	38.726038789695785	19.034747196805576
35	17.431748411142024	26.55000574032988	36.22960199446128	19.788643854066816
36	18.2269007725644	28.44198113251575	34.34388029498761	18.987237799932235
37	19.15216727787957	27.88334902979091	35.360805400950376	17.603678291379143
38	18.169777489879753	28.092614388906885	33.72084449119676	20.016763630016605
39	20.0397249495271	26.253562038031784	34.70435434356294	19.002358668878173
40	19.903077096830497	25.735438929890503	34.87759096556082	19.48389300771818
41	16.94536046004777	28.1865131182872	33.66456125678689	21.203565164878142
42	18.096226596488414	27.75976859470191	33.244070425913804	20.89993438289587
43	18.84228280318509	27.075222589458143	33.32452838289774	20.757966224459032
44	18.149429653890778	26.442946254644767	33.732045134860414	21.67557895660404
45	19.01971966655684	25.222822804883855	33.721964555563126	22.03549297299618
46	18.939821741756095	26.57754065600303	34.538958172129625	19.94367943011125
47	17.31190152394091	25.414913843715553	37.0101935191209	20.26299111322264
48	17.160319479692767	24.10359848679304	37.47912713383929	21.256954899674902
49	18.41917848879049	23.69253486433687	38.044572961459515	19.84371368541312
50	17.254031531678688	23.795207431253715	36.88119943959446	22.069561597473133
51	16.464666169482538	24.427577104764286	35.55373648805685	23.554020237696328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	329.0
1	649.0
2	969.0
3	47322.0
4	93675.0
5	63281.5
6	32888.0
7	30935.0
8	28982.0
9	28147.5
10	27313.0
11	26020.0
12	24727.0
13	22913.5
14	21100.0
15	19208.5
16	17317.0
17	15892.0
18	14467.0
19	12989.0
20	11511.0
21	10695.0
22	9879.0
23	9251.0
24	8623.0
25	8688.5
26	9301.0
27	9848.0
28	10753.0
29	11658.0
30	12226.5
31	12795.0
32	14485.5
33	16176.0
34	18248.0
35	20320.0
36	21839.5
37	23359.0
38	24329.0
39	25299.0
40	29756.0
41	34213.0
42	40053.0
43	45893.0
44	50677.5
45	55462.0
46	61589.0
47	67716.0
48	73981.5
49	80247.0
50	78945.5
51	77644.0
52	68330.5
53	59017.0
54	51500.0
55	43983.0
56	40697.5
57	37412.0
58	35988.5
59	34565.0
60	33631.5
61	32698.0
62	30465.0
63	28232.0
64	24559.5
65	20887.0
66	16961.5
67	13036.0
68	10285.5
69	7535.0
70	6524.5
71	5514.0
72	4249.5
73	2985.0
74	2533.5
75	1523.5
76	965.0
77	908.5
78	852.0
79	518.0
80	184.0
81	119.0
82	54.0
83	116.0
84	178.0
85	94.5
86	11.0
87	8.5
88	6.0
89	5.0
90	4.0
91	2.0
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1071367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.05627608742272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68795251065758	25.68074406858882
2	9.784581083388273	6.4688362738451275
3	3.6483849605776877	3.618060615901706
4	1.7387727499240575	2.299094082991074
5	1.04692504007359	1.7303721583754346
6	0.6868897958886762	1.362361124071771
7	0.48555416333251333	1.123542873496197
8	0.34166516911623035	0.9035342527809666
9	0.2870417617018756	0.8539678551093659
>10	3.43656093883421	28.44232118043662
>50	0.7641162417637982	16.367417250603634
>100	0.08550946122088762	4.795839327739148
>500	0.003742838369908919	0.8356148776041445
>1k	0.0020153745068740335	1.1375973384968496
>5k	0.0	0.0
>10k+	2.8791064383914763E-4	4.38069671995913
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46029	4.2962868932868	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	3071	0.2866431390923932	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2858	0.266761996589404	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	1572	0.14672843199389193	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1245	0.11620667801042966	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1187	0.110793033572996	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15671567259398506	0.0
2	0.0	0.0	0.0	0.9402006968667133	0.0
3	0.0	0.0	0.0	1.356397947668726	0.0
4	0.0	0.0	0.0	1.764288054420194	0.0
5	0.0	0.0	0.0	2.8289092346506846	0.0
6	0.0	0.0	0.0	3.36252656652669	0.0
7	0.0	0.0	0.0	3.964747840842587	0.0
8	0.0	0.0	0.0	4.8607059952378595	0.0
9	0.0	0.0	0.0	5.215953076770145	0.0
10	0.0	0.0	0.0	5.97059644360896	0.0
11	0.0	0.0	0.0	7.044271477467571	0.0
12	0.0	0.0	0.0	7.912414700098099	0.0
13	0.0	0.0	0.0	8.239100140288063	0.0
14	0.0	0.0	0.0	8.375374638195876	0.0
15	0.0	0.0	0.0	8.627389120628132	0.0
16	0.0	0.0	0.0	9.16968695134347	0.0
17	0.0	0.0	0.0	9.855259682256406	0.0
18	0.0	0.0	0.0	10.608689646031658	0.0
19	0.0	0.0	0.0	11.053448538175994	0.0
20	0.0	0.0	0.0	11.500167542961469	0.0
21	0.0	0.0	0.0	12.042652051071201	0.0
22	0.0	0.0	0.0	12.62237869936259	0.0
23	0.0	0.0	0.0	13.14638214542729	0.0
24	0.0	0.0	0.0	13.600754923382931	0.0
25	0.0	0.0	0.0	14.002951369605373	0.0
26	0.0	0.0	0.0	14.360251902475996	0.0
27	0.0	0.0	0.0	14.72921977249626	0.0
28	0.0	0.0	0.0	15.103601286953957	0.0
29	0.0	0.0	0.0	15.47452926961536	0.0
30	0.0	0.0	0.0	15.907994179398843	0.0
31	0.0	0.0	0.0	16.329511735941093	0.0
32	0.0	0.0	0.0	16.72657455381769	0.0
33	0.0	0.0	0.0	17.115983598524128	0.0
34	0.0	0.0	0.0	17.501565756645483	0.0
35	0.0	0.0	0.0	17.883601044273345	0.0
36	0.0	0.0	0.0	18.266009686689994	0.0
37	0.0	0.0	0.0	18.649445054775814	0.0
38	0.0	0.0	0.0	19.0535082749422	0.0
39	0.0	0.0	0.0	19.44049051352151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGA	20	7.033071E-4	45.000004	10
ATAGGCG	20	7.033071E-4	45.000004	1
TAGACCG	20	7.033071E-4	45.000004	1
TACGCTA	20	7.033071E-4	45.000004	1
CGCTAAC	20	7.033071E-4	45.000004	31
TACCTAG	40	6.8139343E-9	45.000004	2
ACGTCGA	20	7.033071E-4	45.000004	24
TAGATCG	40	6.8139343E-9	45.000004	1
GTATACG	20	7.033071E-4	45.000004	1
CGCCCGT	20	7.033071E-4	45.000004	20
TAACGCG	25	3.8906277E-5	45.0	1
GACCCGT	35	1.211829E-7	45.0	10
TTACGCG	25	3.8906277E-5	45.0	1
TGCGTAG	55	1.8189894E-12	45.0	1
GTACCTA	30	2.1653632E-6	44.999996	1
TATACGG	30	2.1653632E-6	44.999996	2
CGTTTTT	29135	0.0	44.54436	1
GCGATAT	80	0.0	42.187504	9
CCGTAGG	60	3.6379788E-12	41.249996	1
GTTTTTT	32230	0.0	40.343624	2
>>END_MODULE