##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934889.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	870754
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44677945780324	34.0	31.0	34.0	30.0	34.0
2	32.61576633584227	34.0	31.0	34.0	31.0	34.0
3	32.67925843579243	34.0	31.0	34.0	31.0	34.0
4	36.11966870091897	37.0	37.0	37.0	35.0	37.0
5	35.9843836491133	37.0	35.0	37.0	35.0	37.0
6	35.96939319256644	37.0	35.0	37.0	35.0	37.0
7	36.13563072923007	37.0	36.0	37.0	35.0	37.0
8	36.13856611626246	37.0	37.0	37.0	35.0	37.0
9	37.91813302034788	39.0	38.0	39.0	35.0	39.0
10	37.47816375233418	39.0	37.0	39.0	34.0	39.0
11	37.40372596623156	39.0	37.0	39.0	34.0	39.0
12	37.27494332498042	39.0	37.0	39.0	34.0	39.0
13	37.18655785675403	39.0	37.0	39.0	34.0	39.0
14	38.3972132198072	40.0	38.0	41.0	34.0	41.0
15	38.45058420633153	40.0	38.0	41.0	34.0	41.0
16	38.5315473715883	40.0	38.0	41.0	34.0	41.0
17	38.543516308854166	40.0	38.0	41.0	34.0	41.0
18	38.08067031561153	39.0	38.0	40.0	34.0	41.0
19	37.61985589500594	38.0	37.0	40.0	34.0	41.0
20	37.10403282672259	38.0	35.0	40.0	34.0	41.0
21	37.07026094626037	38.0	35.0	40.0	34.0	41.0
22	36.88121214487674	38.0	35.0	40.0	33.0	41.0
23	36.7594636372615	37.0	35.0	40.0	33.0	41.0
24	36.527298180657226	37.0	35.0	40.0	33.0	41.0
25	36.39962147747814	37.0	35.0	40.0	33.0	41.0
26	36.55038506857275	37.0	35.0	40.0	33.0	41.0
27	36.6098461792883	37.0	35.0	40.0	33.0	41.0
28	36.54659180434428	37.0	35.0	40.0	33.0	41.0
29	36.457666574026646	37.0	35.0	40.0	33.0	41.0
30	36.17846027695538	37.0	35.0	40.0	32.0	41.0
31	35.67486339425371	37.0	35.0	40.0	30.0	41.0
32	34.97084136277295	37.0	35.0	40.0	24.0	41.0
33	34.01476651270049	37.0	34.0	40.0	18.0	41.0
34	33.23932706596811	37.0	33.0	40.0	12.0	41.0
35	32.77425771228154	37.0	33.0	40.0	10.0	41.0
36	32.54985449392136	37.0	32.0	40.0	9.0	41.0
37	32.3566644540249	37.0	31.0	40.0	8.0	41.0
38	32.24251395916642	37.0	31.0	40.0	8.0	41.0
39	32.22504863600971	37.0	31.0	40.0	8.0	41.0
40	32.138939356006404	37.0	31.0	40.0	8.0	41.0
41	31.99650992128661	37.0	31.0	40.0	8.0	41.0
42	31.841360476093133	37.0	30.0	40.0	8.0	41.0
43	31.704089788849664	36.0	29.0	40.0	7.0	41.0
44	31.556602668491905	36.0	29.0	40.0	7.0	41.0
45	31.522544828964325	36.0	28.0	40.0	7.0	41.0
46	31.42649014532233	36.0	28.0	40.0	7.0	41.0
47	31.36475169795373	35.0	28.0	40.0	7.0	41.0
48	31.294889256896898	35.0	28.0	40.0	7.0	41.0
49	31.134854390562662	35.0	27.0	40.0	7.0	41.0
50	30.940001424053175	35.0	27.0	40.0	7.0	41.0
51	30.27236509967224	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	5.0
12	12.0
13	13.0
14	30.0
15	36.0
16	87.0
17	157.0
18	316.0
19	625.0
20	1024.0
21	1785.0
22	2822.0
23	4835.0
24	9194.0
25	19569.0
26	34373.0
27	42439.0
28	38001.0
29	30391.0
30	24715.0
31	22594.0
32	23070.0
33	28872.0
34	53930.0
35	67121.0
36	61627.0
37	83002.0
38	150187.0
39	169821.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.5476632895169	15.981896149773645	18.465490827489738	35.00494973321972
2	49.463798041697196	18.542780165236106	18.24901177600103	13.74441001706567
3	22.29573450136319	18.85710545113775	45.66306901834502	13.184091029154043
4	22.10233889250006	19.76597293839592	44.35236588060463	13.779322288499394
5	18.970340647301075	21.99025212631811	43.74725812341948	15.292149102961341
6	19.960631820238554	24.640598837329485	43.64114319314066	11.757626149291305
7	58.38434276500596	5.214101801427269	31.801633986177496	4.599921447389274
8	57.908777909719625	4.833397262602297	31.853657864333666	5.404166963344411
9	53.46435388180818	6.559717210601387	33.91210376294568	6.063825144644756
10	30.48518869853024	18.705397850598445	39.57374872811379	11.235664722757518
11	25.638239962147747	18.030580393543985	41.270554025591615	15.060625618716653
12	22.286202532517795	16.209629815079804	46.73639167893573	14.767775973466673
13	18.637181109704922	19.916187579959438	46.95482306139277	14.491808248942869
14	14.950835712497446	22.600412975421303	46.28838914320233	16.160362168878926
15	15.021349313353713	19.162128454190277	51.18977346070187	14.626748771754135
16	15.855913380817086	19.171315894041257	48.64014405905686	16.332626666084796
17	15.812502727521204	19.394455839421926	45.320607197899754	19.472434235157117
18	16.829896848019075	19.802722697799837	47.767222430215654	15.600158023965438
19	18.175397414195054	20.905674851909954	45.10837733734212	15.810550396552872
20	18.53347788238699	21.514916957028046	45.27777075959456	14.673834400990406
21	17.392168166899033	21.607135884532255	46.00943550072696	14.991260447841755
22	16.247642847463233	20.734214255691043	44.19583487414356	18.822308022702163
23	16.414280037760378	20.100395748971582	45.455203191716606	18.030121021551437
24	17.614963583285288	18.607207087191103	46.161028258268125	17.616801071255487
25	15.75898589038925	20.26772199725755	44.880413986039684	19.09287812631352
26	15.619796176646904	21.34713133674953	45.648828486576	17.384244000027564
27	17.536296129561276	21.243313266433457	44.4102467516658	16.81014385233947
28	14.203552323618382	22.30262508125142	45.819944553800504	17.6738780413297
29	17.037418145653078	19.608752873946028	44.69471285805176	18.659116122349136
30	18.153003029558292	20.70228790220889	42.49466554273653	18.650043525496294
31	18.525783401511795	21.884252039037435	41.702937913578346	17.887026645872428
32	18.922795646072256	21.61333740643167	40.880087831924975	18.583779115571105
33	18.53439662637209	23.197941094729398	38.84196914398326	19.425693134915257
34	16.967593602785634	23.82624713753827	39.75072178824329	19.455437471432806
35	18.29988722417583	23.306812256963504	37.216366505350535	21.176934013510127
36	19.940993667557084	26.91035585251403	34.86116629955188	18.287484180377007
37	18.456533073635033	26.83743054869688	37.0676448227628	17.63839155490529
38	16.984705209508082	27.92832418800258	34.22470640387526	20.862264198614074
39	18.646368549555902	25.2014920402318	35.52530335777958	20.626836052432722
40	19.752306621617585	22.702049028772763	37.28159732829249	20.264047021317158
41	17.7796484426141	25.025552567085533	35.249335633255775	21.945463357044584
42	19.07806337955381	25.15486578298808	34.676728444543464	21.090342392914646
43	20.028159503143254	24.94286560842672	33.74316971268579	21.28580517574424
44	17.93778725104909	24.805743068650848	33.869956382629304	23.386513297670756
45	18.34949939937112	23.502849254783786	33.64428989128962	24.503361454555478
46	19.26709495448772	23.810513646793467	35.531390036680854	21.391001362037958
47	16.87215907133358	23.393978092549673	38.59815745893789	21.13570537717886
48	16.603885827684973	22.486373878271017	37.704449247433836	23.20529104661018
49	17.762766521887926	21.539723044625692	38.77742737903013	21.920083054456253
50	17.066817953176212	21.222641526768754	38.17875082974066	23.531789690314373
51	16.348130470833325	21.371937424347177	36.072645086901694	26.207287017917803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	663.5
2	864.0
3	32177.5
4	63491.0
5	42826.0
6	22161.0
7	21018.0
8	19875.0
9	19994.0
10	20113.0
11	19599.0
12	19085.0
13	18171.5
14	17258.0
15	16005.0
16	14752.0
17	13597.5
18	12443.0
19	11229.5
20	10016.0
21	9255.5
22	8495.0
23	8032.0
24	7569.0
25	7661.0
26	7723.0
27	7693.0
28	8168.5
29	8644.0
30	9126.5
31	9609.0
32	11359.0
33	13109.0
34	13890.0
35	14671.0
36	15714.0
37	16757.0
38	18529.5
39	20302.0
40	23630.0
41	26958.0
42	32024.0
43	37090.0
44	42310.0
45	47530.0
46	55667.5
47	63805.0
48	72159.0
49	80513.0
50	79826.0
51	79139.0
52	66353.0
53	53567.0
54	46420.5
55	39274.0
56	35365.5
57	31457.0
58	28754.5
59	26052.0
60	25372.5
61	24693.0
62	21311.5
63	17930.0
64	14799.5
65	11669.0
66	9105.0
67	6541.0
68	5307.5
69	4074.0
70	3130.5
71	2187.0
72	1830.5
73	1474.0
74	1075.0
75	497.0
76	318.0
77	343.0
78	368.0
79	282.0
80	196.0
81	130.0
82	64.0
83	54.0
84	44.0
85	25.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	870754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.855303928414074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28747587031471	26.2416785346296
2	10.595285816884386	7.386038147118406
3	4.299560887510607	4.495875044787119
4	2.2821134621680175	3.1817503329196617
5	1.3372961901227838	2.330593257451992
6	0.8667286269050741	1.812605382854002
7	0.607240538877832	1.4815887478168543
8	0.43558792677995184	1.2146039660370398
9	0.3052864596355623	0.9576767102244335
>10	3.3991890859177825	28.318496960695725
>50	0.49986056551674607	11.19358701703636
>100	0.07664303512181452	4.5972965220447595
>500	0.0053784586050394274	1.220884108359594
>1k	0.0020169219768897853	1.8415197246725277
>5k	0.0	0.0
>10k+	3.361536628149642E-4	3.7258055433518935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31799	3.6518924977663034	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4603	0.528622320425746	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4492	0.5158747476325115	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2602	0.2988214811531156	TruSeq Adapter, Index 20 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1520	0.1745613571686148	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1409	0.1618137843753804	TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1091	0.125293710967736	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	974	0.11185708018567816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31822994783831027	0.0
2	0.0	0.0	0.0	1.757327557496147	0.0
3	0.0	0.0	0.0	2.461544822073743	0.0
4	0.0	0.0	0.0	3.1324576171915375	0.0
5	0.0	0.0	0.0	4.830066815656316	0.0
6	0.0	0.0	0.0	5.6638269821327265	0.0
7	0.0	0.0	0.0	6.5850975131897185	0.0
8	0.0	0.0	0.0	8.026147453815888	0.0
9	0.0	0.0	0.0	8.560626767146633	0.0
10	0.0	0.0	0.0	9.737078440064588	0.0
11	0.0	0.0	0.0	11.388405910280056	0.0
12	0.0	0.0	0.0	12.743668131297703	0.0
13	0.0	0.0	0.0	13.281133362580016	0.0
14	0.0	0.0	0.0	13.497153042076178	0.0
15	0.0	0.0	0.0	13.899448064550953	0.0
16	0.0	0.0	0.0	14.799128111958142	0.0
17	0.0	0.0	0.0	15.815144116478363	0.0
18	0.0	0.0	0.0	17.070148400122193	0.0
19	0.0	0.0	0.0	17.71372856168332	0.0
20	0.0	0.0	0.0	18.348006440395334	0.0
21	0.0	0.0	0.0	19.121588875847827	0.0
22	0.0	0.0	0.0	19.927442193776887	0.0
23	0.0	0.0	0.0	20.692985619359774	0.0
24	0.0	0.0	0.0	21.32887130004571	0.0
25	0.0	0.0	0.0	21.847846808627924	0.0
26	0.0	0.0	0.0	22.36728168920269	0.0
27	0.0	0.0	0.0	22.856053489274814	0.0
28	0.0	0.0	0.0	23.354701787186738	0.0
29	1.1484299813724657E-4	0.0	0.0	23.848526679176896	0.0
30	1.1484299813724657E-4	0.0	0.0	24.393341862339994	0.0
31	1.1484299813724657E-4	0.0	0.0	24.93907578948819	0.0
32	2.2968599627449314E-4	0.0	0.0	25.533732833842855	0.0
33	2.2968599627449314E-4	0.0	0.0	26.04696619251821	0.0
34	2.2968599627449314E-4	0.0	0.0	26.52459822177102	0.0
35	2.2968599627449314E-4	0.0	0.0	27.005216168975394	0.0
36	2.2968599627449314E-4	0.0	0.0	27.517875312660063	0.0
37	2.2968599627449314E-4	0.0	0.0	27.992521423961303	0.0
38	2.2968599627449314E-4	0.0	0.0	28.5247038773293	0.0
39	2.2968599627449314E-4	0.0	0.0	29.00704446950574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	30	2.164892E-6	45.000004	35
GTACCGG	40	6.8121153E-9	45.000004	2
GTCGATT	20	7.032343E-4	45.000004	8
TCACCGA	20	7.032343E-4	45.000004	24
CAACGAA	20	7.032343E-4	45.000004	36
GGATGCG	20	7.032343E-4	45.000004	15
ACGTTAC	20	7.032343E-4	45.000004	29
CCGATAT	20	7.032343E-4	45.000004	11
TTAGCGA	35	1.2114651E-7	45.000004	27
CGGTTGT	20	7.032343E-4	45.000004	14
TAGCGTA	20	7.032343E-4	45.000004	16
CTATACG	20	7.032343E-4	45.000004	1
GCACGTA	20	7.032343E-4	45.000004	18
GCGCCTA	20	7.032343E-4	45.000004	9
CACGAGA	20	7.032343E-4	45.000004	11
GTTCGCC	20	7.032343E-4	45.000004	32
TCGCTAG	20	7.032343E-4	45.000004	15
CGTACAG	20	7.032343E-4	45.000004	1
AGTTACG	35	1.2114651E-7	45.000004	1
TAAGGCG	40	6.8121153E-9	45.000004	1
>>END_MODULE