##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934888.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1791014
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59496910688582	34.0	31.0	34.0	31.0	34.0
2	32.76030058949846	34.0	31.0	34.0	31.0	34.0
3	32.811624029739576	34.0	31.0	34.0	31.0	34.0
4	36.20943108205743	37.0	37.0	37.0	35.0	37.0
5	36.10207513732444	37.0	35.0	37.0	35.0	37.0
6	36.08770171534114	37.0	36.0	37.0	35.0	37.0
7	36.23391609445822	37.0	37.0	37.0	35.0	37.0
8	36.25368813420777	37.0	37.0	37.0	35.0	37.0
9	38.04338715386926	39.0	39.0	39.0	35.0	39.0
10	37.57580119418385	39.0	38.0	39.0	35.0	39.0
11	37.50164264489278	39.0	37.0	39.0	35.0	39.0
12	37.49127254169984	39.0	37.0	39.0	35.0	39.0
13	37.498228936233886	39.0	37.0	39.0	35.0	39.0
14	38.818013147859254	40.0	38.0	41.0	35.0	41.0
15	38.841078294195356	40.0	38.0	41.0	35.0	41.0
16	38.918015716236724	40.0	38.0	41.0	35.0	41.0
17	38.944759225779364	40.0	38.0	41.0	35.0	41.0
18	38.41975048771255	39.0	38.0	41.0	35.0	41.0
19	37.90404597619003	38.0	37.0	41.0	35.0	41.0
20	37.32567305448199	38.0	35.0	41.0	34.0	41.0
21	37.27821948907155	38.0	35.0	41.0	34.0	41.0
22	37.1073542697042	38.0	35.0	40.0	34.0	41.0
23	37.00079731370051	38.0	35.0	40.0	34.0	41.0
24	36.796722415346835	37.0	35.0	40.0	33.0	41.0
25	36.66350290952499	37.0	35.0	40.0	33.0	41.0
26	36.783251833877344	38.0	35.0	40.0	33.0	41.0
27	36.818329169956236	38.0	35.0	40.0	33.0	41.0
28	36.77002859832475	38.0	35.0	40.0	33.0	41.0
29	36.62994817460947	38.0	35.0	40.0	33.0	41.0
30	36.34596044475364	38.0	35.0	40.0	33.0	41.0
31	35.79795411984496	38.0	35.0	40.0	31.0	41.0
32	34.99129375873109	38.0	35.0	40.0	23.0	41.0
33	33.94150296982603	38.0	34.0	40.0	16.0	41.0
34	33.03709072067555	38.0	33.0	40.0	10.0	41.0
35	32.50253934363439	38.0	32.0	41.0	8.0	41.0
36	32.2708633210014	38.0	31.0	41.0	8.0	41.0
37	32.02768878411894	38.0	30.0	40.0	7.0	41.0
38	31.891310174013157	38.0	30.0	40.0	7.0	41.0
39	31.823728346065412	37.0	29.0	40.0	7.0	41.0
40	31.706263044286644	37.0	29.0	40.0	7.0	41.0
41	31.60383335920322	37.0	28.0	40.0	7.0	41.0
42	31.478272643318256	37.0	27.0	40.0	7.0	41.0
43	31.36857389166137	37.0	26.0	40.0	7.0	41.0
44	31.26466571450586	37.0	25.0	40.0	7.0	41.0
45	31.235682691480914	37.0	24.0	40.0	7.0	41.0
46	31.179248738424157	37.0	24.0	40.0	7.0	41.0
47	31.12816259392724	37.0	24.0	40.0	7.0	41.0
48	31.04867968647928	36.0	24.0	40.0	7.0	41.0
49	30.901766820359864	36.0	24.0	40.0	7.0	41.0
50	30.79908197255856	36.0	24.0	40.0	7.0	41.0
51	30.256906422842032	35.0	23.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	13.0
11	12.0
12	22.0
13	24.0
14	33.0
15	87.0
16	137.0
17	263.0
18	585.0
19	1030.0
20	1900.0
21	3073.0
22	5332.0
23	9500.0
24	19799.0
25	44558.0
26	79738.0
27	96197.0
28	85422.0
29	66109.0
30	50647.0
31	43224.0
32	42249.0
33	48867.0
34	83165.0
35	112044.0
36	117510.0
37	161106.0
38	304677.0
39	413447.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.966942748632896	16.342976660148945	18.332073339460216	36.35800725175794
2	51.14063876664281	17.86049690287178	18.03743577660476	12.96142855388065
3	23.14013179126461	17.809241022124898	46.32761106278343	12.72301612382706
4	20.595986407699773	19.113418432240064	46.599468234195825	13.691126925864342
5	19.02140351778378	21.60117118012478	45.28244893674756	14.094976365343879
6	19.585385708877766	25.07719090973047	44.26844234606765	11.068981035324125
7	58.79083022243265	4.62173941688898	32.960267200591396	3.627163160086967
8	59.87127962148816	4.0250383302419745	32.457702731525266	3.645979316744593
9	55.348478571356786	5.960589922803507	34.02726053509353	4.663670970746181
10	29.306638585739698	19.619779633213362	40.487734880911034	10.5858469001359
11	22.28363374043977	19.503644304287963	43.648570027928315	14.564151927343952
12	20.67482442906644	16.713437192562424	47.69711459541913	14.914623782952004
13	18.37277653887686	18.49600282298184	47.942059637724775	15.189161000416524
14	15.173024889811023	20.507656556565163	47.75400973973403	16.565308813889786
15	14.310887575418171	20.128709211653288	50.95080217128398	14.609601041644565
16	16.33426651047954	19.090917212260763	49.53222029531874	15.042595981940957
17	16.33599737355487	19.197784048589234	47.12715813500062	17.339060442855274
18	16.53700082746422	20.072316575973165	48.10034985767839	15.29033273888423
19	17.496624817003106	21.229258956099727	46.22096756362597	15.053148663271196
20	18.770875046202878	20.147581202603664	47.063339538384405	14.018204212809056
21	17.49416810812199	20.435406981743302	47.34842943717916	14.721995472955543
22	16.499312679856214	19.493649965885247	46.716106071755995	17.290931282502537
23	16.202274242412397	20.336412780692946	47.12514810046153	16.336164876433127
24	16.842079403064407	18.56171978555165	47.866069165288486	16.730131646095455
25	15.928462870753663	20.057855494150242	46.43051366432647	17.58316797076963
26	15.249294533711073	21.34595262795266	47.05524356593527	16.349509272400997
27	15.451303004889969	21.384366621366443	47.63167680431309	15.532653569430503
28	14.264712615311772	20.704360769932563	48.15143823554701	16.87948837920865
29	15.104572046896338	19.915422213338367	48.08616794731923	16.893837792446067
30	15.520872533659706	20.184655172991388	46.80532927157465	17.489143021774257
31	15.549962200183806	21.519373941242222	44.281507570571755	18.649156288002217
32	16.37385302404113	22.761351949230992	43.36275428332777	17.50204074340011
33	17.217844193289388	23.19122016913324	40.74658266211208	18.844352975465295
34	16.00629587485078	24.017344364700666	39.91816926054179	20.058190499906754
35	16.764804741894814	24.504721906138087	37.80947552615446	20.92099782581264
36	17.717337776254123	26.120343001227237	36.50937401941023	19.65294520310841
37	17.52872953533585	26.679523443144497	36.63946792152378	19.152279099995866
38	17.35765326234189	27.19615815398428	34.48554841000684	20.960640173666985
39	18.706274769488125	25.495278093861913	35.1069282540505	20.691518882599468
40	18.524422477992914	24.522253874062404	36.40021797707891	20.55310567086578
41	17.037331924820244	25.015717353409855	35.964487156437634	21.982463565332264
42	18.255803695560168	25.19014368396897	35.40396669149432	21.150085928976548
43	18.674058382569875	25.62207777270306	35.14969732229899	20.554166522428076
44	17.650224956365502	25.75669425252957	34.64981289928499	21.94326789181994
45	17.69941496828054	24.92654998788396	34.42083646470658	22.953198579128916
46	17.643413172649684	25.22420260254805	35.08453870265671	22.047845522145558
47	16.772342371416414	24.66329129755546	37.137007304242175	21.427359026785943
48	17.192160418623192	23.337785187608805	36.98954893708257	22.48050545668543
49	17.395006404193378	22.967659660951842	37.46335874538111	22.173975189473673
50	16.869940715147955	22.794517519126035	37.22617466976808	23.109367095957932
51	16.29942591180192	22.72790720787219	35.70418768362503	25.268479196700866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	776.5
2	1203.0
3	67661.5
4	134120.0
5	93666.5
6	53213.0
7	51295.0
8	49377.0
9	48415.5
10	47454.0
11	45345.5
12	43237.0
13	41042.5
14	38848.0
15	36140.5
16	33433.0
17	30808.5
18	28184.0
19	25836.5
20	23489.0
21	21769.5
22	20050.0
23	18555.5
24	17061.0
25	16549.0
26	17335.0
27	18633.0
28	19586.5
29	20540.0
30	23601.5
31	26663.0
32	28606.5
33	30550.0
34	33503.0
35	36456.0
36	39773.0
37	43090.0
38	47280.0
39	51470.0
40	56968.5
41	62467.0
42	66685.5
43	70904.0
44	79597.0
45	88290.0
46	92256.0
47	96222.0
48	106585.5
49	116949.0
50	125123.0
51	133297.0
52	129153.0
53	125009.0
54	106336.0
55	87663.0
56	76925.5
57	66188.0
58	61170.0
59	56152.0
60	53172.5
61	50193.0
62	45383.5
63	40574.0
64	33297.0
65	26020.0
66	20173.0
67	14326.0
68	11895.5
69	9465.0
70	7828.0
71	6191.0
72	4939.5
73	3688.0
74	2819.0
75	1395.0
76	840.0
77	760.0
78	680.0
79	478.0
80	276.0
81	209.5
82	143.0
83	88.0
84	33.0
85	27.0
86	21.0
87	13.5
88	6.0
89	6.5
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1791014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.65251730177474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45564268739969	26.840329983451998
2	9.747647685423027	6.755610601414521
3	3.7134550713508028	3.8604169832804054
4	1.8310413967667063	2.5380077472689635
5	1.0855968430069203	1.8809331692524676
6	0.7201931504867156	1.4973903364716343
7	0.5148835606226131	1.248940804501214
8	0.3905828049510813	1.0827741925074443
9	0.3076149990097304	0.9593670667923092
>10	3.5531540872057206	29.24368622546461
>50	0.5976509822606272	13.209773596002858
>100	0.07627647487052734	4.932907192317806
>500	0.003953846302673876	0.902386903417052
>1k	0.002141666747281683	1.37438885935127
>5k	0.0	0.0
>10k+	1.6474359594474485E-4	3.673086338505506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64341	3.5924342299948524	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	4152	0.23182398350878328	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	3507	0.19581086468335815	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2478	0.13835737744093568	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1847	0.1031259387140469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1166858550519427E-4	0.0	0.0	0.14583917266978372	0.0
2	1.1166858550519427E-4	0.0	0.0	0.7698432284728093	0.0
3	1.1166858550519427E-4	0.0	0.0	1.128690227993751	0.0
4	1.1166858550519427E-4	0.0	0.0	1.4973082287463972	0.0
5	1.1166858550519427E-4	0.0	0.0	2.4391210788972058	0.0
6	1.1166858550519427E-4	0.0	0.0	2.94615229138354	0.0
7	1.1166858550519427E-4	0.0	0.0	3.455528544165484	0.0
8	1.1166858550519427E-4	0.0	0.0	4.211078193693628	0.0
9	1.1166858550519427E-4	0.0	0.0	4.489691314529088	0.0
10	1.1166858550519427E-4	0.0	0.0	5.190746694330698	0.0
11	1.1166858550519427E-4	0.0	0.0	6.279403734420836	0.0
12	1.1166858550519427E-4	0.0	0.0	7.184254282769426	0.0
13	1.1166858550519427E-4	0.0	0.0	7.5074231692214575	0.0
14	1.675028782577914E-4	0.0	0.0	7.638689591482813	0.0
15	1.675028782577914E-4	0.0	0.0	7.89228894916511	0.0
16	1.675028782577914E-4	0.0	0.0	8.44181005843617	0.0
17	1.675028782577914E-4	0.0	0.0	9.140743735113182	0.0
18	2.2333717101038854E-4	0.0	0.0	9.893222498539933	0.0
19	2.2333717101038854E-4	0.0	0.0	10.356088785458963	0.0
20	2.2333717101038854E-4	0.0	0.0	10.817224209302664	0.0
21	2.2333717101038854E-4	0.0	0.0	11.405326814865768	0.0
22	2.2333717101038854E-4	0.0	0.0	12.05892304582767	0.0
23	2.2333717101038854E-4	0.0	0.0	12.721341095044483	0.0
24	2.2333717101038854E-4	0.0	0.0	13.21915964922664	0.0
25	3.350057565155828E-4	0.0	0.0	13.67968089585006	0.0
26	3.350057565155828E-4	0.0	0.0	14.089727941825133	0.0
27	3.350057565155828E-4	0.0	0.0	14.511946863620274	0.0
28	3.350057565155828E-4	0.0	0.0	14.948794370116593	0.0
29	3.350057565155828E-4	0.0	0.0	15.393402843305525	0.0
30	3.350057565155828E-4	0.0	0.0	15.880612881864687	0.0
31	3.350057565155828E-4	0.0	0.0	16.363914519931168	0.0
32	3.350057565155828E-4	0.0	0.0	16.833815927737025	0.0
33	3.350057565155828E-4	0.0	0.0	17.275409349117314	0.0
34	3.350057565155828E-4	0.0	0.0	17.712033518442624	0.0
35	3.350057565155828E-4	0.0	0.0	18.164905466958942	0.0
36	3.350057565155828E-4	0.0	0.0	18.6295026169533	0.0
37	3.350057565155828E-4	0.0	0.0	19.10934252886912	0.0
38	3.908400492681799E-4	0.0	0.0	19.567295398025923	0.0
39	3.908400492681799E-4	0.0	0.0	20.01530976307276	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	20	7.0343434E-4	45.0	1
ATCGTTC	20	7.0343434E-4	45.0	22
ATACGGT	20	7.0343434E-4	45.0	23
TCGAATC	20	7.0343434E-4	45.0	25
TTACGAG	60	0.0	44.999996	1
CGTTTTT	50165	0.0	44.520084	1
GCGTAAG	80	0.0	42.1875	1
TATACGG	75	0.0	42.000004	2
GACGTAG	55	6.184564E-11	40.909092	1
TTCGTAC	55	6.184564E-11	40.909092	33
CGTACAT	55	6.184564E-11	40.909092	35
GTTTTTT	56395	0.0	39.741554	2
TCTAGCG	85	0.0	39.705883	1
AGTACGG	210	0.0	39.642857	2
CCGCTAG	40	3.4596633E-7	39.375	3
ATATGCG	70	0.0	38.571426	1
CCCGTAG	35	6.250244E-6	38.571426	44
TACGGGA	285	0.0	37.894737	4
TACGAGG	180	0.0	37.5	2
TGCACGG	235	0.0	37.340427	2
>>END_MODULE