SRR2934886.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934886.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526511
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16573	3.1477025171363944	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3844	0.7300892099120436	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3681	0.6991306924261791	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2118	0.40227079776111047	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1666	0.31642263884325306	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	1154	0.21917870661771552	Illumina PCR Primer Index 2 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1063	0.2018951171010672	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	761	0.1445363914524103	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	643	0.12212470394730596	Illumina PCR Primer Index 2 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	606	0.11509731040756983	Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	558	0.10598069176142569	Illumina PCR Primer Index 2 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8879152E-5	45.000004	25
CAGCGCA	20	7.0297986E-4	45.000004	28
AACCGAG	20	7.0297986E-4	45.000004	40
CGTATAG	20	7.0297986E-4	45.000004	14
CGACGGT	25	3.8879152E-5	45.000004	28
CTATACG	20	7.0297986E-4	45.000004	1
GGCGTAT	20	7.0297986E-4	45.000004	1
GAATCGT	25	3.8879152E-5	45.000004	8
ACCGAAC	20	7.0297986E-4	45.000004	22
CGCTGCG	20	7.0297986E-4	45.000004	45
CCTAAGG	20	7.0297986E-4	45.000004	2
ACGGGCC	20	7.0297986E-4	45.000004	5
TAGTTCG	20	7.0297986E-4	45.000004	21
ACGATTG	20	7.0297986E-4	45.000004	1
AGACGAT	20	7.0297986E-4	45.000004	39
CTAACGT	20	7.0297986E-4	45.000004	38
CTTATCA	20	7.0297986E-4	45.000004	30
AGGTTCG	25	3.8879152E-5	45.000004	1
CGCTAAG	20	7.0297986E-4	45.000004	1
GCTTACG	20	7.0297986E-4	45.000004	1