##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934886.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526511
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36003806188285	33.0	31.0	34.0	30.0	34.0
2	32.54545109218991	34.0	31.0	34.0	31.0	34.0
3	32.62240485004112	34.0	31.0	34.0	31.0	34.0
4	36.06109843858913	37.0	35.0	37.0	35.0	37.0
5	35.916412002788164	37.0	35.0	37.0	35.0	37.0
6	35.906868042642984	37.0	35.0	37.0	35.0	37.0
7	36.10038156847625	37.0	35.0	37.0	35.0	37.0
8	36.108901808319295	37.0	36.0	37.0	35.0	37.0
9	37.89886441118989	39.0	38.0	39.0	35.0	39.0
10	37.378138348486544	39.0	37.0	39.0	34.0	39.0
11	37.25483608129745	39.0	37.0	39.0	34.0	39.0
12	37.21372392979444	39.0	37.0	39.0	34.0	39.0
13	37.127786503985675	39.0	37.0	39.0	33.0	39.0
14	38.29708591083567	40.0	38.0	41.0	33.0	41.0
15	38.33153913213589	40.0	37.0	41.0	34.0	41.0
16	38.45590880342481	40.0	38.0	41.0	34.0	41.0
17	38.50393818932558	40.0	38.0	41.0	34.0	41.0
18	38.080714363042745	39.0	37.0	40.0	34.0	41.0
19	37.703692800340356	38.0	37.0	40.0	34.0	41.0
20	37.22026510367305	38.0	35.0	40.0	34.0	41.0
21	37.16411812858611	38.0	35.0	40.0	34.0	41.0
22	36.966063387089726	38.0	35.0	40.0	33.0	41.0
23	36.8483564445947	38.0	35.0	40.0	33.0	41.0
24	36.61162064990095	37.0	35.0	40.0	33.0	41.0
25	36.47390462877319	37.0	35.0	40.0	33.0	41.0
26	36.67020442118019	38.0	35.0	40.0	33.0	41.0
27	36.75692055816498	38.0	35.0	40.0	33.0	41.0
28	36.73956859400848	38.0	35.0	40.0	33.0	41.0
29	36.675966883882765	38.0	35.0	40.0	33.0	41.0
30	36.385638666618554	38.0	35.0	40.0	33.0	41.0
31	35.89757099091947	38.0	35.0	40.0	31.0	41.0
32	35.30667165548298	38.0	35.0	40.0	27.0	41.0
33	34.517288337755524	38.0	35.0	40.0	20.0	41.0
34	33.83855797884565	38.0	34.0	40.0	15.0	41.0
35	33.43514380516267	38.0	34.0	40.0	10.0	41.0
36	33.2876312175814	38.0	33.0	40.0	10.0	41.0
37	33.08175897559595	38.0	33.0	40.0	10.0	41.0
38	32.982139024635764	38.0	33.0	40.0	10.0	41.0
39	32.93576582445571	38.0	33.0	40.0	10.0	41.0
40	32.7619252019426	38.0	33.0	40.0	10.0	41.0
41	32.631908924979726	38.0	32.0	40.0	9.0	41.0
42	32.57166516938867	38.0	32.0	40.0	8.0	41.0
43	32.49129837743181	38.0	32.0	40.0	8.0	41.0
44	32.36014822102482	37.0	31.0	40.0	8.0	41.0
45	32.29661488553895	37.0	31.0	40.0	8.0	41.0
46	32.19690186909675	37.0	31.0	40.0	8.0	41.0
47	32.14317839513325	37.0	31.0	40.0	8.0	41.0
48	32.133174805464655	37.0	31.0	40.0	8.0	41.0
49	32.082323066374684	37.0	31.0	40.0	8.0	41.0
50	31.977968171605152	37.0	31.0	40.0	8.0	41.0
51	31.349971795461062	35.0	29.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	9.0
12	6.0
13	10.0
14	16.0
15	26.0
16	61.0
17	99.0
18	189.0
19	316.0
20	615.0
21	975.0
22	1578.0
23	2729.0
24	5226.0
25	10854.0
26	18909.0
27	22416.0
28	19926.0
29	16262.0
30	13176.0
31	12375.0
32	13149.0
33	16634.0
34	30543.0
35	43197.0
36	39119.0
37	53944.0
38	99968.0
39	104129.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.57203173343007	18.12364793897943	20.024462926700487	32.27985740089001
2	46.36199433630067	19.213273796748787	19.975271171922333	14.449460695028213
3	23.827232479473363	18.94775227867984	43.47050678903195	13.75450845281485
4	23.205403115984282	19.89303167455191	42.3937961410113	14.507769068452511
5	18.831705320496628	22.27721738007373	42.898438968986405	15.992638330443237
6	19.951529977531337	24.932432560763214	42.008429073656586	13.107608388048872
7	60.555240061461205	4.855359147292269	29.45617470480199	5.133226086444537
8	60.28762931828585	3.799730679890829	29.583237577182626	6.329402424640701
9	55.887531314635396	5.434644290432678	32.02022369903003	6.65760069590189
10	30.716167373521163	18.969024388854177	38.78589431179975	11.528913925824911
11	22.66999170007844	19.587435020350952	41.847558740463164	15.895014539107443
12	20.93080676377132	17.547401668721072	45.84329672124609	15.67849484626152
13	17.51986188322751	21.871907709430573	46.100271409334276	14.50795899800764
14	14.370260070539837	24.05685731162312	43.827384423117465	17.745498194719577
15	12.140866952447338	21.41265804513106	50.97595301902524	15.470521983396356
16	13.734755779081539	21.25976475325302	46.79294449688611	18.212534970779338
17	14.328285638856547	20.456172805506437	43.35445983084874	21.861081724788274
18	15.440323184131005	20.756641361718938	47.12703058435626	16.676004869793793
19	16.134705637678984	20.83071388821886	44.00249947294548	19.03208100115667
20	17.075426724227984	21.99289283604711	44.175905156777354	16.755775282947553
21	16.559578052500328	22.345022231254426	45.01159519934056	16.083804516904678
22	15.014501121534025	21.3328876319773	42.097316105456486	21.55529514103219
23	13.797052673163524	21.509142259136084	43.78047182300085	20.91333324469954
24	16.82377006368338	19.203777318992383	44.61122369713074	19.3612289201935
25	13.957923006356943	21.90134679047541	43.34173455065516	20.798995652512485
26	13.615669948016281	22.3210911073083	42.861022846626184	21.202216098049234
27	15.408984807534884	21.98092727407405	42.95978621529275	19.65030170309832
28	12.604295066959665	20.490739984539736	46.62656620659397	20.278398741906628
29	15.07091019940704	18.52895760962259	44.13070192265688	22.269430268313485
30	15.93527960479458	19.949440752424927	43.99490229074036	20.120377352040126
31	15.933190379688172	23.495235617109614	40.767049501339955	19.804524501862257
32	17.3753254917751	24.132449274564063	38.16273544142478	20.32948979223606
33	16.224922176364785	22.443025881700475	38.833566630136886	22.498485311797854
34	15.172142652290265	23.973288307366797	38.64914503210759	22.205424008235344
35	16.108305429516193	24.790555183082592	34.549705514224776	24.55143387317644
36	17.72365629587986	24.10908793928332	34.988062927460206	23.17919283737662
37	17.015598914362663	25.547614389822815	36.94300783839274	20.49377885742178
38	17.305431415487995	27.21158722229925	34.460438623314616	21.022542738898142
39	19.04841494289768	24.435766774103485	35.933912111997664	20.581906171001176
40	18.973012909511862	24.741743287414696	35.513408076944266	20.77183572612918
41	16.118751555048235	26.324806129406603	31.98128814022879	25.57515417531637
42	18.332000660954854	23.82647276125285	32.47263589934493	25.368890678447364
43	20.683898342104914	22.662014658763066	33.03862597362638	23.615461025505642
44	18.435892127609872	23.04111405079856	34.04696198180095	24.476031839790622
45	19.11963852607068	23.32069035594698	32.66674390468575	24.892927213296588
46	19.689807050564944	23.93967077610914	35.03098700691913	21.33953516640678
47	15.828159335702388	23.85610177185282	37.341859904161545	22.973878988283243
48	16.562996784492633	21.953007629470232	36.02621787578987	25.45777771024727
49	17.805705863695156	19.822377880044293	38.90308084731373	23.468835408946823
50	18.148148851590946	20.739927560867674	37.0499381779298	24.061985409611573
51	16.369648497372324	21.331748054646532	33.59416992237579	28.704433525605356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	451.5
2	483.0
3	15137.5
4	29792.0
5	20310.0
6	10828.0
7	10771.5
8	10715.0
9	11000.0
10	11285.0
11	11255.5
12	11226.0
13	10520.0
14	9814.0
15	9119.0
16	8424.0
17	7736.0
18	7048.0
19	6409.5
20	5771.0
21	5278.0
22	4785.0
23	4396.0
24	4007.0
25	4158.0
26	4554.0
27	4799.0
28	4882.0
29	4965.0
30	5443.0
31	5921.0
32	6398.0
33	6875.0
34	7775.5
35	8676.0
36	9498.5
37	10321.0
38	11559.0
39	12797.0
40	15051.0
41	17305.0
42	21012.0
43	24719.0
44	29407.5
45	34096.0
46	39564.5
47	45033.0
48	52644.5
49	60256.0
50	58239.5
51	56223.0
52	45798.0
53	35373.0
54	29251.0
55	23129.0
56	19397.5
57	15666.0
58	13614.5
59	11563.0
60	10534.0
61	9505.0
62	8094.5
63	6684.0
64	5974.0
65	5264.0
66	4459.5
67	3655.0
68	2732.0
69	1809.0
70	1504.5
71	1200.0
72	1061.0
73	922.0
74	656.5
75	314.5
76	238.0
77	180.5
78	123.0
79	87.0
80	51.0
81	35.0
82	19.0
83	16.0
84	13.0
85	12.0
86	11.0
87	6.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.32796468869844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83305822293272	28.306937195816456
2	10.690159683351261	7.980838063533673
3	4.218729470548492	4.724297541234168
4	2.1377781708214685	3.191956322907765
5	1.2647374862789125	2.3605038114146235
6	0.7643354411781159	1.7118651815170471
7	0.5550050018889611	1.4502044978793505
8	0.40788050014079097	1.2180279125171285
9	0.31965925646123855	1.0739006493840642
>10	3.387359802420018	29.744935474765104
>50	0.3642247151632669	8.402842679790327
>100	0.05084618530768882	3.4061076868523594
>500	0.002594193127943307	0.5982524958598362
>1k	0.003113031753531968	2.61960610521209
>5k	0.0	0.0
>10k+	5.188386255886614E-4	3.2097243813159815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16573	3.1477025171363944	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3844	0.7300892099120436	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3681	0.6991306924261791	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2118	0.40227079776111047	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1666	0.31642263884325306	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	1154	0.21917870661771552	Illumina PCR Primer Index 2 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1063	0.2018951171010672	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	761	0.1445363914524103	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	643	0.12212470394730596	Illumina PCR Primer Index 2 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	606	0.11509731040756983	Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	558	0.10598069176142569	Illumina PCR Primer Index 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8992955512800303E-4	0.0	0.0	0.43133001969569484	0.0
2	1.8992955512800303E-4	0.0	0.0	2.5232141398755203	0.0
3	1.8992955512800303E-4	0.0	0.0	3.52091409296292	0.0
4	1.8992955512800303E-4	0.0	0.0	4.465813629724735	0.0
5	1.8992955512800303E-4	0.0	0.0	6.9616779136618225	0.0
6	1.8992955512800303E-4	0.0	0.0	8.141710239672106	0.0
7	1.8992955512800303E-4	0.0	0.0	9.454123465606607	0.0
8	1.8992955512800303E-4	0.0	0.0	11.395583378125053	0.0
9	1.8992955512800303E-4	0.0	0.0	12.059956961962808	0.0
10	1.8992955512800303E-4	0.0	0.0	13.48063003432027	0.0
11	1.8992955512800303E-4	0.0	0.0	15.681913578253825	0.0
12	1.8992955512800303E-4	0.0	0.0	17.41350133235583	0.0
13	1.8992955512800303E-4	0.0	0.0	18.081483577741015	0.0
14	1.8992955512800303E-4	0.0	0.0	18.309778903004876	0.0
15	1.8992955512800303E-4	0.0	0.0	18.79219997303	0.0
16	1.8992955512800303E-4	0.0	0.0	19.80756337474431	0.0
17	1.8992955512800303E-4	0.0	0.0	21.046283933289143	0.0
18	1.8992955512800303E-4	0.0	0.0	22.49981481868375	0.0
19	1.8992955512800303E-4	0.0	0.0	23.28954190890599	0.0
20	1.8992955512800303E-4	0.0	0.0	24.028747737464176	0.0
21	1.8992955512800303E-4	0.0	0.0	24.845444824514587	0.0
22	1.8992955512800303E-4	0.0	0.0	25.70981422990213	0.0
23	1.8992955512800303E-4	0.0	0.0	26.617677503413983	0.0
24	1.8992955512800303E-4	0.0	0.0	27.247293978663315	0.0
25	1.8992955512800303E-4	0.0	0.0	27.80967539139733	0.0
26	1.8992955512800303E-4	0.0	0.0	28.328942795117293	0.0
27	1.8992955512800303E-4	0.0	0.0	28.821999920229587	0.0
28	3.7985911025600606E-4	0.0	0.0	29.315436904452138	0.0
29	3.7985911025600606E-4	0.0	0.0	29.824827971305442	0.0
30	3.7985911025600606E-4	0.0	0.0	30.40791170554841	0.0
31	5.697886653840091E-4	0.0	0.0	30.93572593924913	0.0
32	5.697886653840091E-4	0.0	0.0	31.43068235991271	0.0
33	5.697886653840091E-4	0.0	0.0	31.900758008854517	0.0
34	5.697886653840091E-4	0.0	0.0	32.382989149324516	0.0
35	5.697886653840091E-4	0.0	0.0	32.92181929722266	0.0
36	7.597182205120121E-4	0.0	0.0	33.35371910558374	0.0
37	7.597182205120121E-4	0.0	0.0	33.80347229212685	0.0
38	9.496477756400151E-4	0.0	0.0	34.27240836373789	0.0
39	9.496477756400151E-4	0.0	0.0	34.694431835232315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8879152E-5	45.000004	25
CAGCGCA	20	7.0297986E-4	45.000004	28
AACCGAG	20	7.0297986E-4	45.000004	40
CGTATAG	20	7.0297986E-4	45.000004	14
CGACGGT	25	3.8879152E-5	45.000004	28
CTATACG	20	7.0297986E-4	45.000004	1
GGCGTAT	20	7.0297986E-4	45.000004	1
GAATCGT	25	3.8879152E-5	45.000004	8
ACCGAAC	20	7.0297986E-4	45.000004	22
CGCTGCG	20	7.0297986E-4	45.000004	45
CCTAAGG	20	7.0297986E-4	45.000004	2
ACGGGCC	20	7.0297986E-4	45.000004	5
TAGTTCG	20	7.0297986E-4	45.000004	21
ACGATTG	20	7.0297986E-4	45.000004	1
AGACGAT	20	7.0297986E-4	45.000004	39
CTAACGT	20	7.0297986E-4	45.000004	38
CTTATCA	20	7.0297986E-4	45.000004	30
AGGTTCG	25	3.8879152E-5	45.000004	1
CGCTAAG	20	7.0297986E-4	45.000004	1
GCTTACG	20	7.0297986E-4	45.000004	1
>>END_MODULE