##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934884.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574664
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29442770036056	33.0	31.0	34.0	30.0	34.0
2	32.484758049921346	34.0	31.0	34.0	31.0	34.0
3	32.57302876115435	34.0	31.0	34.0	31.0	34.0
4	36.0363673381315	37.0	35.0	37.0	35.0	37.0
5	35.88387823145351	37.0	35.0	37.0	35.0	37.0
6	35.87075752091657	37.0	35.0	37.0	35.0	37.0
7	36.08248298135954	37.0	35.0	37.0	35.0	37.0
8	36.06613255745966	37.0	35.0	37.0	35.0	37.0
9	37.89135738448903	39.0	38.0	39.0	35.0	39.0
10	37.38186662119082	39.0	37.0	39.0	34.0	39.0
11	37.228265560397034	39.0	37.0	39.0	34.0	39.0
12	37.18433206186572	39.0	37.0	39.0	34.0	39.0
13	37.08876143276767	39.0	37.0	39.0	33.0	39.0
14	38.208565352971476	40.0	37.0	41.0	33.0	41.0
15	38.27665905642254	40.0	37.0	41.0	33.0	41.0
16	38.403084237049825	40.0	37.0	41.0	34.0	41.0
17	38.43573810087286	40.0	37.0	41.0	34.0	41.0
18	38.06819463199365	39.0	37.0	40.0	34.0	41.0
19	37.69402642239639	38.0	37.0	40.0	34.0	41.0
20	37.209712110032996	38.0	35.0	40.0	34.0	41.0
21	37.133110826500356	38.0	35.0	40.0	34.0	41.0
22	36.95433505491905	38.0	35.0	40.0	33.0	41.0
23	36.81273230966269	38.0	35.0	40.0	33.0	41.0
24	36.594420391741956	37.0	35.0	40.0	33.0	41.0
25	36.47514025587126	37.0	35.0	40.0	33.0	41.0
26	36.67463248089318	38.0	35.0	40.0	33.0	41.0
27	36.71529102223212	38.0	35.0	40.0	33.0	41.0
28	36.65647752425765	38.0	35.0	40.0	33.0	41.0
29	36.62780337727785	38.0	35.0	40.0	33.0	41.0
30	36.27447169128395	38.0	35.0	40.0	32.0	41.0
31	35.82593828741665	38.0	35.0	40.0	31.0	41.0
32	35.25966129773224	38.0	35.0	40.0	27.0	41.0
33	34.433985772555786	38.0	34.0	40.0	21.0	41.0
34	33.83181650494898	38.0	34.0	40.0	15.0	41.0
35	33.4530421254855	38.0	33.0	40.0	12.0	41.0
36	33.242861915832556	38.0	33.0	40.0	10.0	41.0
37	33.0548999067281	37.0	33.0	40.0	10.0	41.0
38	32.8863649019253	37.0	33.0	40.0	10.0	41.0
39	32.863504587028245	37.0	33.0	40.0	10.0	41.0
40	32.73586478359528	37.0	33.0	40.0	10.0	41.0
41	32.654478095025965	37.0	32.0	40.0	10.0	41.0
42	32.55787729873457	37.0	32.0	40.0	8.0	41.0
43	32.44020505895619	37.0	32.0	40.0	8.0	41.0
44	32.241301003716956	37.0	31.0	40.0	8.0	41.0
45	32.17389465844389	37.0	31.0	40.0	8.0	41.0
46	32.05295094176771	36.0	31.0	40.0	8.0	41.0
47	31.989903665446242	36.0	31.0	40.0	8.0	41.0
48	31.971557292609248	36.0	31.0	40.0	8.0	41.0
49	31.87200520652068	36.0	31.0	40.0	8.0	41.0
50	31.722340358887976	36.0	30.0	40.0	8.0	41.0
51	30.950872161819778	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	3.0
12	4.0
13	7.0
14	10.0
15	22.0
16	40.0
17	86.0
18	183.0
19	383.0
20	635.0
21	1005.0
22	1683.0
23	3033.0
24	5645.0
25	11656.0
26	20368.0
27	24661.0
28	21661.0
29	17178.0
30	14264.0
31	13874.0
32	15094.0
33	19466.0
34	33952.0
35	48579.0
36	47748.0
37	63332.0
38	110563.0
39	99469.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.33703172636532	17.50605571255551	20.537740314340205	31.619172246738962
2	45.29063939971879	19.981937271170633	20.19667144627121	14.530751882839363
3	22.671508916514693	18.773230966268986	44.7343143123634	13.820945804852922
4	22.88084863502847	19.52932496206479	43.37386716411677	14.21595923878997
5	19.16267592889062	22.595638494842206	42.726184344242895	15.51550123202428
6	19.538895772138154	25.016183369760412	41.38522684560021	14.059694012501216
7	59.569417955535755	4.536041930589005	30.69950440605293	5.195035707822311
8	58.87927554188187	4.168000779585984	30.131868361338103	6.820855317194047
9	55.066438823382015	4.96481422187574	32.272597552656855	7.696149402085392
10	30.889006445505547	18.170617961104227	38.85783692731753	12.082538666072697
11	22.229163476396643	19.12195648239667	42.327168571547894	16.32171146965879
12	20.212854816031626	17.00645942672588	46.11964556680078	16.66104019044172
13	16.730124037698552	21.46106246432698	47.14128603845029	14.667527459524173
14	13.492231982515	24.901507663608648	44.36383695515988	17.24242339871647
15	12.107248757534837	20.790757729734246	51.43231523116116	15.669678281569752
16	12.65539515264572	20.986350284688097	47.59320228864171	18.765052274024473
17	12.968969693594865	20.345280024501275	43.84857934375566	22.837170938148205
18	14.177675998496515	19.658617905419515	48.741873512174074	17.421832583909904
19	15.545257750616011	20.604387955396543	44.365751117174554	19.484603176812882
20	17.180648170061115	21.947781660239723	44.85856082858854	16.01300934111063
21	16.044157977531217	22.45538262358526	45.455257332980665	16.04520206590286
22	14.551111606086339	20.88298553589576	42.295149861484276	22.270752996533627
23	13.292289059345983	22.095346150098145	43.67091030584829	20.941454484707585
24	16.233660016983837	19.74614731390865	44.65652972867623	19.36366294043128
25	13.237474419834893	22.356194228279485	43.48819484081133	20.918136511074298
26	15.596766110283575	21.509438558879623	43.90113179179485	18.992663539041946
27	18.275722857182632	22.874584104798632	42.20605432043768	16.643638717581055
28	13.339621065526986	22.445637798783288	45.039884175796644	19.174856959893084
29	18.42502749432712	19.129439115726754	43.993011568499156	18.452521821446968
30	19.189300182367436	21.141745437333817	42.92699734105494	16.7419570392438
31	18.329667423050687	23.93241963999833	40.655061044366796	17.082851892584188
32	21.51831331003856	24.9055100023666	36.85510141578383	16.721075271811007
33	18.731989475589213	22.66959475450002	37.74727492935002	20.851140840560745
34	17.28662313978255	23.036591817131402	39.14391714114683	20.53286790193922
35	19.507399106260355	23.690017123049294	34.93397881196664	21.868604958723704
36	21.958570573413333	23.581431932398758	35.200917405649214	19.259080088538695
37	19.339126863697743	25.6064413291941	37.545069814709116	17.509361992399036
38	17.290625478540502	28.54659418373171	33.47086297384211	20.69191736388568
39	18.9757841103671	23.509389834755616	37.6179819859953	19.89684406888199
40	20.09713502150822	22.341576991076526	38.125408934612224	19.435879052803028
41	18.187323375050465	24.955452229476702	32.73425862765024	24.12296576782259
42	20.185186474183176	23.70359027188061	32.94934083220804	23.161882421728176
43	22.703875656035528	22.87214789859814	32.45009257583562	21.973883869530717
44	19.19469463895424	23.801386549357538	33.72944886055156	23.274469951136663
45	19.878572522378292	22.56535993206465	33.86274414266423	23.693323402892823
46	21.781423579691786	22.808284493199505	35.70225383876491	19.708038088343795
47	16.39984408280317	22.839259114891483	39.118336976041654	21.642559826263692
48	17.755766848106024	21.39772110311417	36.77696880263945	24.069543246140356
49	19.137269778514053	19.278743752871243	39.535102251054525	22.048884217560175
50	19.01719961577548	19.73414029763479	37.50121810310025	23.74744198348948
51	17.15611209332758	20.204328094329902	34.35433575097796	28.285224061364556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	560.0
1	544.5
2	529.0
3	16776.5
4	33024.0
5	22278.5
6	11533.0
7	11364.0
8	11195.0
9	11580.0
10	11965.0
11	11925.5
12	11886.0
13	11400.0
14	10914.0
15	10120.0
16	9326.0
17	8473.0
18	7620.0
19	6936.5
20	6253.0
21	5735.5
22	5218.0
23	4849.0
24	4480.0
25	4365.5
26	4202.0
27	4153.0
28	4729.0
29	5305.0
30	5948.0
31	6591.0
32	7101.0
33	7611.0
34	8140.0
35	8669.0
36	9834.0
37	10999.0
38	11772.5
39	12546.0
40	15805.5
41	19065.0
42	22836.5
43	26608.0
44	32379.5
45	38151.0
46	47677.5
47	57204.0
48	63508.0
49	69812.0
50	68376.0
51	66940.0
52	52751.5
53	38563.0
54	31223.0
55	23883.0
56	19314.5
57	14746.0
58	12696.0
59	10646.0
60	9555.0
61	8464.0
62	7284.5
63	6105.0
64	5050.5
65	3996.0
66	3332.5
67	2669.0
68	2075.5
69	1482.0
70	1091.5
71	701.0
72	607.5
73	514.0
74	356.0
75	178.0
76	158.0
77	104.0
78	50.0
79	51.0
80	52.0
81	32.0
82	12.0
83	12.0
84	12.0
85	7.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	574664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.07650072024032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58369345015852	26.54642169752587
2	11.077935366023064	7.993062864222137
3	4.851445277025851	5.2506950709248885
4	2.534243347005876	3.657065277340869
5	1.5457268168655647	2.7882207310972658
6	1.0197199181019765	2.2072755815909613
7	0.6603746188199138	1.667680378803953
8	0.5012202444995563	1.4465818009349813
9	0.3944843818593886	1.2808454477646412
>10	3.4434167678302154	27.751177666856297
>50	0.31657237799701904	7.191046340615532
>100	0.06331447559926671	4.339204708529663
>500	0.002944859330198452	0.776084805251184
>1k	0.004417288995297678	3.585430349425331
>5k	0.0	0.0
>10k+	4.908098883664087E-4	3.519207279116423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19875	3.4585427310567565	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	4880	0.8491918756003507	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	4674	0.8133448415073852	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2506	0.4360809098882129	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2041	0.35516406108613036	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	1512	0.2631102696532234	TruSeq Adapter, Index 27 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1294	0.22517505881697827	TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	1143	0.19889883479737724	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	1124	0.1955925549538513	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	1075	0.18706583325212645	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	982	0.17088246349170993	No Hit
GCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	866	0.15069675497334095	No Hit
AGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	690	0.12007016273857418	TruSeq Adapter, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	689	0.11989614800996756	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	628	0.10928124956496318	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46183508972199405	0.0
2	0.0	0.0	0.0	2.8521014018626536	0.0
3	0.0	0.0	0.0	3.9833711525343505	0.0
4	0.0	0.0	0.0	5.013886375342809	0.0
5	0.0	0.0	0.0	7.834143081870449	0.0
6	0.0	0.0	0.0	9.054160344131528	0.0
7	0.0	0.0	0.0	10.547206715576406	0.0
8	0.0	0.0	0.0	12.67958319992204	0.0
9	0.0	0.0	0.0	13.440027563933011	0.0
10	0.0	0.0	0.0	15.310859911182883	0.0
11	0.0	0.0	0.0	17.875314966658777	0.0
12	0.0	0.0	0.0	19.965231857224396	0.0
13	0.0	0.0	0.0	20.73281082510824	0.0
14	1.7401472860662927E-4	0.0	0.0	21.01558475909401	0.0
15	1.7401472860662927E-4	0.0	0.0	21.59070343713892	0.0
16	1.7401472860662927E-4	0.0	0.0	22.81263486141467	0.0
17	1.7401472860662927E-4	0.0	0.0	24.281145156126016	0.0
18	1.7401472860662927E-4	0.0	0.0	26.02407667785001	0.0
19	1.7401472860662927E-4	0.0	0.0	27.01752676346526	0.0
20	5.220441858198878E-4	0.0	0.0	27.825303133657233	0.0
21	5.220441858198878E-4	0.0	0.0	28.81614299834338	0.0
22	5.220441858198878E-4	0.0	0.0	29.89868862500522	0.0
23	5.220441858198878E-4	0.0	0.0	31.001767989642644	0.0
24	5.220441858198878E-4	0.0	0.0	31.83773474586889	0.0
25	5.220441858198878E-4	0.0	0.0	32.521612629292946	0.0
26	5.220441858198878E-4	0.0	0.0	33.170861581724274	0.0
27	5.220441858198878E-4	0.0	0.0	33.772604513246	0.0
28	5.220441858198878E-4	0.0	0.0	34.36286247267969	0.0
29	5.220441858198878E-4	0.0	0.0	35.00776105689586	0.0
30	5.220441858198878E-4	0.0	0.0	35.70033967675024	0.0
31	5.220441858198878E-4	0.0	0.0	36.350980747010425	0.0
32	5.220441858198878E-4	0.0	0.0	36.93201592582796	0.0
33	5.220441858198878E-4	0.0	0.0	37.50052204418582	0.0
34	5.220441858198878E-4	0.0	0.0	38.066243926885974	0.0
35	5.220441858198878E-4	0.0	0.0	38.70835827544443	0.0
36	5.220441858198878E-4	0.0	0.0	39.286435203875655	0.0
37	5.220441858198878E-4	0.0	0.0	39.80325894783734	0.0
38	5.220441858198878E-4	0.0	0.0	40.29972296855206	0.0
39	5.220441858198878E-4	0.0	0.0	40.788182311750866	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	70	0.0	45.000004	6
AGGGTTA	35	1.210501E-7	45.000004	6
TACGGGT	35	1.210501E-7	45.000004	4
CGTTCGG	30	2.1635988E-6	45.000004	2
TTGCGAG	35	1.210501E-7	45.000004	1
AGCGAAA	30	2.1635988E-6	45.000004	19
TAGGCTA	30	2.1635988E-6	45.000004	24
ATAACGG	35	1.210501E-7	45.000004	2
TGTGTCG	35	1.210501E-7	45.000004	1
TATCGAG	30	2.1635988E-6	45.000004	1
GCGTAAG	30	2.1635988E-6	45.000004	1
GTTATCC	30	2.1635988E-6	45.000004	12
GATAGGC	30	2.1635988E-6	45.000004	22
CTAGCGA	30	2.1635988E-6	45.000004	17
GTTGATC	55	1.8189894E-12	45.0	16
CTTCGGA	20	7.0303393E-4	45.0	34
TAGAGTC	25	3.8883627E-5	45.0	13
TAATACG	45	3.8380676E-10	45.0	1
GATACGA	20	7.0303393E-4	45.0	29
GCCCATC	20	7.0303393E-4	45.0	21
>>END_MODULE