SRR2934883.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934883.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1636000
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58328	3.565281173594132	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5461	0.33380195599022006	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5049	0.30861858190709046	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2850	0.17420537897310512	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2018	0.12334963325183375	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1781	0.10886308068459659	TruSeq Adapter, Index 10 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	80	0.0	45.0	28
GTAATCG	40	6.8175723E-9	45.0	1
CTCGGTA	20	7.034165E-4	45.0	45
TGTTACG	50	2.1827873E-11	45.0	1
GCTCGAT	20	7.034165E-4	45.0	29
TCGACTT	20	7.034165E-4	45.0	38
CGAATTG	30	2.1660671E-6	44.999996	1
CGTTTTT	41425	0.0	44.48944	1
GCGATAC	80	0.0	42.1875	9
AAGCGCC	45	1.9292202E-8	40.000004	42
GTTTTTT	46660	0.0	39.695667	2
TCGTTCA	70	0.0	38.571426	16
GCATCGC	35	6.249913E-6	38.571426	22
TACGGGA	350	0.0	37.928574	4
GCGATCA	155	0.0	37.741936	9
TAGAACG	30	1.14013324E-4	37.499996	1
CCGTCCC	30	1.14013324E-4	37.499996	39
TTAAGCG	60	1.5643309E-10	37.499996	1
CTCGACA	30	1.14013324E-4	37.499996	35
TCGACAT	30	1.14013324E-4	37.499996	36