##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934883.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1636000
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.594731662591684	34.0	31.0	34.0	31.0	34.0
2	32.75331295843521	34.0	31.0	34.0	31.0	34.0
3	32.812963936430315	34.0	31.0	34.0	31.0	34.0
4	36.205797677261614	37.0	37.0	37.0	35.0	37.0
5	36.092633863080685	37.0	35.0	37.0	35.0	37.0
6	36.08520232273839	37.0	35.0	37.0	35.0	37.0
7	36.23969926650367	37.0	37.0	37.0	35.0	37.0
8	36.254349633251834	37.0	37.0	37.0	35.0	37.0
9	38.03464914425428	39.0	39.0	39.0	35.0	39.0
10	37.51496699266504	39.0	37.0	39.0	34.0	39.0
11	37.42448838630807	39.0	37.0	39.0	34.0	39.0
12	37.435924205378974	39.0	37.0	39.0	35.0	39.0
13	37.46025122249389	39.0	37.0	39.0	35.0	39.0
14	38.75187163814181	40.0	38.0	41.0	35.0	41.0
15	38.78897738386308	40.0	38.0	41.0	35.0	41.0
16	38.86823655256724	40.0	38.0	41.0	35.0	41.0
17	38.86851894865526	40.0	38.0	41.0	35.0	41.0
18	38.39322371638142	39.0	38.0	41.0	35.0	41.0
19	37.935117970660144	39.0	37.0	41.0	35.0	41.0
20	37.416097799511	38.0	35.0	41.0	34.0	41.0
21	37.37139547677262	38.0	35.0	41.0	34.0	41.0
22	37.19420782396088	38.0	35.0	41.0	34.0	41.0
23	37.08296760391198	38.0	35.0	40.0	34.0	41.0
24	36.89910696821516	38.0	35.0	40.0	33.0	41.0
25	36.77053850855746	38.0	35.0	40.0	33.0	41.0
26	36.91073716381418	38.0	35.0	40.0	33.0	41.0
27	36.95885085574572	38.0	35.0	40.0	34.0	41.0
28	36.886017114914424	38.0	35.0	40.0	33.0	41.0
29	36.791260391198044	38.0	35.0	40.0	33.0	41.0
30	36.520052567237165	38.0	35.0	40.0	33.0	41.0
31	36.02708985330074	38.0	35.0	41.0	31.0	41.0
32	35.36077444987775	38.0	35.0	41.0	25.0	41.0
33	34.4644706601467	38.0	35.0	41.0	18.0	41.0
34	33.687410146699264	38.0	34.0	41.0	12.0	41.0
35	33.22808190709046	38.0	33.0	41.0	10.0	41.0
36	33.02032579462103	38.0	33.0	41.0	10.0	41.0
37	32.783581907090465	38.0	33.0	41.0	9.0	41.0
38	32.67941503667482	38.0	32.0	41.0	8.0	41.0
39	32.64251100244499	38.0	32.0	41.0	8.0	41.0
40	32.53750794621027	38.0	31.0	41.0	8.0	41.0
41	32.41754951100245	38.0	31.0	41.0	8.0	41.0
42	32.29544498777506	38.0	31.0	40.0	8.0	41.0
43	32.19449511002445	38.0	31.0	40.0	8.0	41.0
44	32.08669070904646	38.0	30.0	40.0	8.0	41.0
45	32.05130012224939	37.0	30.0	40.0	8.0	41.0
46	31.993911369193153	37.0	30.0	40.0	8.0	41.0
47	31.983559290953544	37.0	30.0	40.0	8.0	41.0
48	31.937295843520783	37.0	30.0	40.0	8.0	41.0
49	31.820816014669926	37.0	29.0	40.0	8.0	41.0
50	31.745398533007336	37.0	29.0	40.0	8.0	41.0
51	31.21110391198044	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	3.0
9	9.0
10	17.0
11	12.0
12	18.0
13	36.0
14	45.0
15	69.0
16	109.0
17	232.0
18	508.0
19	926.0
20	1597.0
21	2735.0
22	4243.0
23	7533.0
24	14950.0
25	32867.0
26	60646.0
27	77470.0
28	71536.0
29	56977.0
30	45275.0
31	39251.0
32	38040.0
33	45239.0
34	82709.0
35	105345.0
36	107192.0
37	146219.0
38	289416.0
39	404506.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.309718826405867	15.695537897310514	19.716687041564793	35.278056234718825
2	49.431540342298284	18.16955990220049	19.019682151589244	13.37921760391198
3	23.597860635696822	17.949877750611247	45.19596577017115	13.256295843520782
4	21.81075794621027	19.432273838630806	44.28539119804401	14.471577017114914
5	19.396271393643033	22.148166259168704	43.58832518337408	14.867237163814181
6	20.526772616136917	24.944743276283617	42.55971882640587	11.968765281173594
7	60.77762836185819	4.558618581907091	30.734596577017115	3.929156479217604
8	61.14596577017115	3.8378973105134473	30.669865525672375	4.346271393643032
9	56.58777506112469	5.607518337408313	32.61839853300734	5.186308068459658
10	30.623533007334963	18.805134474327627	40.10268948655257	10.468643031784842
11	22.704462102689487	18.533374083129583	44.60984107579462	14.152322738386308
12	21.29529339853301	16.61790953545232	46.79028117359413	15.296515892420537
13	18.621210268948655	18.23557457212714	47.25189486552567	15.891320293398534
14	15.02817848410758	20.987591687041565	46.537041564792176	17.44718826405868
15	14.537347188264057	19.85281173594132	50.13319070904646	15.476650366748165
16	16.113997555012226	19.674022004889977	48.16369193154034	16.048288508557455
17	16.18416870415648	19.436552567237165	46.153239608801954	18.2260391198044
18	16.88875305623472	20.062958435207822	46.84559902200489	16.20268948655257
19	17.987163814180928	20.874022004889977	45.12970660146699	16.009107579462103
20	19.507701711491443	20.32805623471883	45.2841075794621	14.880134474327628
21	18.269559902200488	20.967542787286064	45.472555012224944	15.29034229828851
22	16.909046454767726	20.011491442542788	45.05709046454768	18.02237163814181
23	16.62988997555012	20.20421760391198	45.67805623471883	17.487836185819074
24	17.396577017114915	18.37976772616137	46.46815403422983	17.755501222493887
25	16.24614914425428	19.79046454767726	44.79248166259169	19.17090464547677
26	15.244926650366747	21.098899755501222	46.09003667481662	17.566136919315404
27	16.937469437652812	20.69559902200489	45.68649144254279	16.68044009779951
28	14.573533007334962	21.41216381418093	46.69963325183374	17.314669926650367
29	16.176100244498777	19.754095354523226	46.89028117359413	17.179523227383864
30	17.4929706601467	20.09981662591687	45.703789731051344	16.703422982885087
31	16.652017114914425	23.09743276283619	42.91234718826406	17.33820293398533
32	17.736674816625918	22.33551344743276	42.59211491442543	17.335696821515892
33	17.700916870415647	23.34993887530562	40.43661369193154	18.512530562347187
34	16.347799511002446	24.705012224938873	39.97793398533007	18.969254278728606
35	16.652506112469435	24.3619804400978	38.0468826405868	20.938630806845964
36	17.990097799511002	27.030073349633255	36.03691931540342	18.942909535452323
37	17.977200488997553	27.952567237163816	36.25171149144254	17.81852078239609
38	16.983129584352078	26.85	35.53129584352078	20.635574572127137
39	17.87078239608802	25.617542787286062	36.516992665036675	19.99468215158924
40	18.367848410757944	25.32127139364303	36.98196821515892	19.328911980440097
41	16.913080684596576	26.441381418092906	34.51253056234719	22.133007334963324
42	17.87389975550122	25.49700488997555	34.44926650366748	22.179828850855746
43	18.969682151589243	25.197432762836186	34.64443765281174	21.188447432762835
44	17.992298288508557	25.571699266503668	35.02933985330073	21.40666259168704
45	18.132579462102687	24.453973105134473	35.18997555012225	22.223471882640585
46	18.151650366748164	25.273899755501223	35.966259168704156	20.608190709046458
47	16.663814180929094	24.747616136919316	37.704889975550124	20.883679706601466
48	16.741075794621025	23.386002444987774	37.48056234718826	22.392359413202932
49	17.161002444987776	22.5158924205379	38.73746943765281	21.585635696821516
50	16.609107579462105	22.45874083129584	38.45385085574572	22.478300733496333
51	16.1450488997555	22.09645476772616	36.87133251833741	24.88716381418093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	731.5
2	1166.0
3	61546.0
4	121926.0
5	84508.0
6	47090.0
7	44363.0
8	41636.0
9	40234.0
10	38832.0
11	37021.0
12	35210.0
13	32835.0
14	30460.0
15	27910.0
16	25360.0
17	23064.0
18	20768.0
19	19213.0
20	17658.0
21	16191.0
22	14724.0
23	13886.5
24	13049.0
25	12787.5
26	14296.5
27	16067.0
28	17655.0
29	19243.0
30	20608.0
31	21973.0
32	24494.5
33	27016.0
34	30097.5
35	33179.0
36	36487.5
37	39796.0
38	44565.0
39	49334.0
40	56657.5
41	63981.0
42	73929.0
43	83877.0
44	98038.0
45	112199.0
46	123628.5
47	135058.0
48	133441.5
49	131825.0
50	112525.5
51	93226.0
52	82355.5
53	71485.0
54	64554.5
55	57624.0
56	55235.5
57	52847.0
58	50831.0
59	48815.0
60	49526.5
61	50238.0
62	45446.0
63	40654.0
64	33577.0
65	26500.0
66	21403.0
67	16306.0
68	13068.0
69	9830.0
70	7726.0
71	5622.0
72	4709.0
73	3796.0
74	3076.0
75	1718.5
76	1081.0
77	919.0
78	757.0
79	565.0
80	373.0
81	270.5
82	168.0
83	99.5
84	31.0
85	28.0
86	25.0
87	16.0
88	7.0
89	4.0
90	1.0
91	3.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1636000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.05512205516149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63795531125712	24.245838895051975
2	10.46783916204873	6.7109572398654285
3	4.024694863188277	3.8703625522284506
4	1.9883184444381263	2.5494316168397186
5	1.1897020928134467	1.9068022897208052
6	0.7683604168773934	1.4777932167215768
7	0.5421724906105156	1.2165583753029683
8	0.4088499141877754	1.0484587121225155
9	0.31711431645406374	0.9148624307436707
>10	3.8792470307487568	30.69033354801065
>50	0.6858475096580534	13.916917228125842
>100	0.08190314307208449	4.997039756834534
>500	0.0054602080506806075	1.1878750562922271
>1k	0.0019500743038145026	0.9637146630983143
>5k	3.900148607629005E-4	0.6569787318650375
>10k+	1.9500743038145026E-4	3.646075687176395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58328	3.565281173594132	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5461	0.33380195599022006	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5049	0.30861858190709046	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2850	0.17420537897310512	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2018	0.12334963325183375	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1781	0.10886308068459659	TruSeq Adapter, Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8337408312958436E-4	0.0	0.0	0.18453545232273838	0.0
2	1.8337408312958436E-4	0.0	0.0	1.0877139364303179	0.0
3	1.8337408312958436E-4	0.0	0.0	1.5789731051344744	0.0
4	1.8337408312958436E-4	0.0	0.0	2.0770171149144256	0.0
5	1.8337408312958436E-4	0.0	0.0	3.300305623471883	0.0
6	1.8337408312958436E-4	0.0	0.0	3.9704156479217603	0.0
7	1.8337408312958436E-4	0.0	0.0	4.694804400977995	0.0
8	1.8337408312958436E-4	0.0	0.0	5.7831295843520785	0.0
9	1.8337408312958436E-4	0.0	0.0	6.19419315403423	0.0
10	1.8337408312958436E-4	0.0	0.0	7.1375916870415645	0.0
11	1.8337408312958436E-4	0.0	0.0	8.489364303178483	0.0
12	1.8337408312958436E-4	0.0	0.0	9.533190709046455	0.0
13	1.8337408312958436E-4	0.0	0.0	9.931601466992666	0.0
14	1.8337408312958436E-4	0.0	0.0	10.094132029339853	0.0
15	1.8337408312958436E-4	0.0	0.0	10.413447432762837	0.0
16	1.8337408312958436E-4	0.0	0.0	11.115892420537897	0.0
17	1.8337408312958436E-4	0.0	0.0	11.952444987775062	0.0
18	1.8337408312958436E-4	0.0	0.0	12.88416870415648	0.0
19	1.8337408312958436E-4	0.0	0.0	13.432457212713937	0.0
20	1.8337408312958436E-4	0.0	0.0	13.95776283618582	0.0
21	1.8337408312958436E-4	0.0	0.0	14.617542787286064	0.0
22	1.8337408312958436E-4	0.0	0.0	15.315525672371638	0.0
23	1.8337408312958436E-4	0.0	0.0	15.999205378973105	0.0
24	1.8337408312958436E-4	0.0	0.0	16.537041564792176	0.0
25	1.8337408312958436E-4	0.0	0.0	17.013691931540343	0.0
26	1.8337408312958436E-4	0.0	0.0	17.456601466992666	0.0
27	1.8337408312958436E-4	0.0	0.0	17.8739608801956	0.0
28	1.8337408312958436E-4	0.0	0.0	18.30690709046455	0.0
29	1.8337408312958436E-4	0.0	0.0	18.75281173594132	0.0
30	1.8337408312958436E-4	0.0	0.0	19.238508557457212	0.0
31	1.8337408312958436E-4	0.0	0.0	19.708557457212713	0.0
32	1.8337408312958436E-4	0.0	0.0	20.166136919315402	0.0
33	1.8337408312958436E-4	0.0	0.0	20.60690709046455	0.0
34	2.4449877750611245E-4	0.0	0.0	21.04486552567237	0.0
35	2.4449877750611245E-4	0.0	0.0	21.50262836185819	0.0
36	2.4449877750611245E-4	0.0	0.0	21.945660146699268	0.0
37	2.4449877750611245E-4	0.0	0.0	22.37713936430318	0.0
38	2.4449877750611245E-4	0.0	0.0	22.79187041564792	0.0
39	3.056234718826406E-4	0.0	0.0	23.224938875305625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	80	0.0	45.0	28
GTAATCG	40	6.8175723E-9	45.0	1
CTCGGTA	20	7.034165E-4	45.0	45
TGTTACG	50	2.1827873E-11	45.0	1
GCTCGAT	20	7.034165E-4	45.0	29
TCGACTT	20	7.034165E-4	45.0	38
CGAATTG	30	2.1660671E-6	44.999996	1
CGTTTTT	41425	0.0	44.48944	1
GCGATAC	80	0.0	42.1875	9
AAGCGCC	45	1.9292202E-8	40.000004	42
GTTTTTT	46660	0.0	39.695667	2
TCGTTCA	70	0.0	38.571426	16
GCATCGC	35	6.249913E-6	38.571426	22
TACGGGA	350	0.0	37.928574	4
GCGATCA	155	0.0	37.741936	9
TAGAACG	30	1.14013324E-4	37.499996	1
CCGTCCC	30	1.14013324E-4	37.499996	39
TTAAGCG	60	1.5643309E-10	37.499996	1
CTCGACA	30	1.14013324E-4	37.499996	35
TCGACAT	30	1.14013324E-4	37.499996	36
>>END_MODULE