##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695090
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64544015882835	34.0	31.0	34.0	31.0	34.0
2	32.79837431124027	34.0	31.0	34.0	31.0	34.0
3	32.83628163259434	34.0	31.0	34.0	31.0	34.0
4	36.22826972046785	37.0	37.0	37.0	35.0	37.0
5	36.12431195960235	37.0	37.0	37.0	35.0	37.0
6	36.11573896905437	37.0	36.0	37.0	35.0	37.0
7	36.23113985239321	37.0	37.0	37.0	35.0	37.0
8	36.229925621142584	37.0	37.0	37.0	35.0	37.0
9	37.995737242659224	39.0	39.0	39.0	35.0	39.0
10	37.63647729071056	39.0	38.0	39.0	35.0	39.0
11	37.55232847544922	39.0	37.0	39.0	35.0	39.0
12	37.477162669582356	39.0	37.0	39.0	35.0	39.0
13	37.43784114287359	39.0	37.0	39.0	35.0	39.0
14	38.75646750780474	40.0	38.0	41.0	35.0	41.0
15	38.81194377706484	40.0	38.0	41.0	35.0	41.0
16	38.89171905796372	40.0	38.0	41.0	35.0	41.0
17	38.9139679753701	40.0	38.0	41.0	35.0	41.0
18	38.378831518220665	39.0	38.0	41.0	35.0	41.0
19	37.88321943920931	38.0	37.0	41.0	35.0	41.0
20	37.29479923463148	38.0	35.0	41.0	34.0	41.0
21	37.2291861485563	38.0	35.0	41.0	34.0	41.0
22	37.021532463422005	38.0	35.0	40.0	34.0	41.0
23	36.91712727848192	38.0	35.0	40.0	34.0	41.0
24	36.72262296968738	37.0	35.0	40.0	33.0	41.0
25	36.55898948337625	37.0	35.0	40.0	33.0	41.0
26	36.68275187385806	37.0	35.0	40.0	33.0	41.0
27	36.724818368844325	37.0	35.0	40.0	33.0	41.0
28	36.641539944467624	37.0	35.0	40.0	33.0	41.0
29	36.52605705735948	37.0	35.0	40.0	33.0	41.0
30	36.22957746478873	37.0	35.0	40.0	33.0	41.0
31	35.72308478038815	37.0	35.0	40.0	31.0	41.0
32	34.99432735329238	37.0	35.0	40.0	23.0	41.0
33	34.01651296954351	37.0	34.0	40.0	17.0	41.0
34	33.20560790688975	37.0	33.0	40.0	12.0	41.0
35	32.68240371750421	37.0	33.0	40.0	10.0	41.0
36	32.40796875224791	37.0	32.0	40.0	8.0	41.0
37	32.15417140226445	37.0	31.0	40.0	8.0	41.0
38	31.995967428678302	37.0	30.0	40.0	8.0	41.0
39	31.905551799047604	37.0	30.0	40.0	7.0	41.0
40	31.762193385029278	37.0	29.0	40.0	7.0	41.0
41	31.605715806586197	37.0	29.0	40.0	7.0	41.0
42	31.471252643542563	36.0	28.0	40.0	7.0	41.0
43	31.339406407803306	36.0	27.0	40.0	7.0	41.0
44	31.21297098217497	36.0	25.0	40.0	7.0	41.0
45	31.17402494640982	36.0	25.0	40.0	7.0	41.0
46	31.1312808413299	36.0	25.0	40.0	7.0	41.0
47	31.10931246313427	35.0	25.0	40.0	7.0	41.0
48	31.05356572530176	35.0	25.0	40.0	7.0	41.0
49	30.916822282006645	35.0	24.0	40.0	7.0	41.0
50	30.83447323368197	35.0	24.0	40.0	7.0	41.0
51	30.29494597821865	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	9.0
12	9.0
13	21.0
14	21.0
15	45.0
16	59.0
17	128.0
18	253.0
19	509.0
20	895.0
21	1365.0
22	2190.0
23	3735.0
24	7044.0
25	14953.0
26	28139.0
27	36389.0
28	33348.0
29	25897.0
30	20491.0
31	18143.0
32	17496.0
33	20370.0
34	35214.0
35	49305.0
36	49338.0
37	60687.0
38	113007.0
39	155904.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.212749428131612	16.191860046900402	17.56909177228848	38.02629875267951
2	51.692586571523115	18.677293587880705	17.279776719561497	12.350343121034687
3	23.21871987800141	17.87883583420852	46.476571379245854	12.425872908544218
4	20.923333669021275	19.131047777985586	46.18970205297156	13.75591650002158
5	19.236501747975083	21.663813319138526	44.58415456991181	14.51553036297458
6	19.44366916514408	24.860233926541888	44.36447078795552	11.331626120358514
7	57.16396437871355	5.019781611014401	33.83403588024572	3.982218130026328
8	56.86788761167618	5.241911119423384	33.71635327799278	4.173847990907652
9	52.148211023032985	6.807032182882793	35.55309384396265	5.491662950121567
10	28.748795120056396	20.882331784373246	40.2988102260139	10.07006286955646
11	23.311369750679766	18.965026111726537	44.50474039333036	13.21886374426333
12	21.525413975168682	16.681005337438318	47.70188033204333	14.091700355349667
13	17.902429901163877	20.26989310736739	47.45414262901207	14.373534362456661
14	14.250960307298335	22.19626235451524	47.10800040282553	16.44477693536089
15	13.669884475391674	19.691406868175342	52.08994518695421	14.548763469478773
16	14.601706253866405	19.574011998446245	50.17594843833173	15.648333309355621
17	15.166525198175776	19.334906271130357	47.01261707117064	18.48595145952323
18	15.088261951689711	20.37405228099958	48.76677840279676	15.770907364513947
19	17.706915651210632	19.848508826195168	46.5961242429038	15.848451279690401
20	18.89812829993238	19.61918600469004	47.28394884115726	14.198736854220318
21	17.531111079140832	20.124156584039476	47.41860766231711	14.926124674502583
22	16.425930455048988	19.60537484354544	45.97879411299256	17.98990058841301
23	15.99447553554216	19.638607950049632	47.533844538117364	16.83307197629084
24	16.320332618797565	18.367837258484514	48.5134299155505	16.798400207167415
25	15.65754075011869	20.33995597692385	47.21546850048195	16.787034772475508
26	14.527471262714181	22.016141794587753	47.201081874289656	16.255305068408408
27	15.636967874663712	21.298680746378167	46.585334273259576	16.479017105698542
28	13.61089930800328	21.4897351422118	48.39862463853601	16.500740911248904
29	15.447352141449308	19.402379547972203	48.185990303413945	16.96427800716454
30	15.378871800774	20.563092549166296	47.06167546648635	16.99636018357335
31	17.273015005251118	22.282725977930916	43.877195758822594	16.56706325799537
32	18.021263433512207	22.06332992849847	43.55191414061488	16.36349249737444
33	18.03119020558489	23.147793810873413	41.660648261376224	17.160367722165475
34	17.40177530967213	23.5595390524968	40.205009423240156	18.833676214590916
35	18.144700686242068	23.13052985944266	39.44079183990563	19.283977614409643
36	19.205426635399732	25.845717820713865	37.177775539858146	17.771080004028256
37	19.10126746176754	27.1931692298839	36.87997237767771	16.82559093067085
38	18.50580500366859	27.30322692025493	36.04928858133479	18.141679494741688
39	20.175372973284038	25.820541224877353	36.7034484742983	17.30063732754032
40	19.810240400523675	25.064811750996274	37.61268324965113	17.512264598828928
41	17.631098131177257	25.93923089096376	36.05763282452632	20.37203815333266
42	19.003150671136112	25.74127091455783	35.56906299903609	19.686515415269966
43	19.9535311973989	25.71868391143593	34.776216029578904	19.55156886158627
44	18.63168798285114	26.620149908644926	34.83160454041923	19.91655756808471
45	19.068897552834883	25.42663539973241	34.50488425959228	20.999582787840424
46	18.483505733070537	25.920959875699552	36.3387475003237	19.256786890906213
47	18.076651944352527	24.604295846581017	38.05277014487333	19.266282064193128
48	18.239364686587347	23.218576011739486	38.24324907565927	20.2988102260139
49	18.242961343135423	23.1175818958696	39.233624422736625	19.405832338258357
50	17.560028197787336	23.435670200981168	38.498611690572446	20.50568991065905
51	16.625616826597994	23.663266627343223	36.802428462501254	22.908688083557525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	546.0
2	803.0
3	30105.5
4	59408.0
5	40964.5
6	22521.0
7	21177.0
8	19833.0
9	19216.0
10	18599.0
11	17786.5
12	16974.0
13	16175.0
14	15376.0
15	13835.5
16	12295.0
17	11094.0
18	9893.0
19	8955.5
20	8018.0
21	7481.5
22	6945.0
23	6488.5
24	6032.0
25	6708.5
26	7484.0
27	7583.0
28	7941.0
29	8299.0
30	9732.5
31	11166.0
32	11720.0
33	12274.0
34	13753.0
35	15232.0
36	15786.0
37	16340.0
38	16898.0
39	17456.0
40	20731.0
41	24006.0
42	26984.5
43	29963.0
44	33098.0
45	36233.0
46	39947.5
47	43662.0
48	47137.5
49	50613.0
50	49402.0
51	48191.0
52	41026.0
53	33861.0
54	30452.5
55	27044.0
56	24067.5
57	21091.0
58	20497.0
59	19903.0
60	19330.5
61	18758.0
62	16699.0
63	14640.0
64	12903.0
65	11166.0
66	9419.5
67	7673.0
68	6601.5
69	5530.0
70	4808.5
71	4087.0
72	3284.0
73	2481.0
74	2087.5
75	1334.0
76	974.0
77	713.5
78	453.0
79	335.5
80	218.0
81	131.5
82	45.0
83	57.0
84	69.0
85	38.5
86	8.0
87	5.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.519223853658985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75388717471058	27.972769479043492
2	9.37090310637381	6.656944102924794
3	3.2258089411604596	3.4373468967063907
4	1.5417229616162889	2.1904321197590026
5	0.9167580563611946	1.6281267311769296
6	0.5970686573096363	1.2724449176990746
7	0.44313634680109876	1.1017901369804621
8	0.3419810206907259	0.9717520342093343
9	0.2823857934922695	0.9027111790930538
>10	4.101669707379425	35.68176882470979
>50	0.3607082377328142	7.968701215279626
>100	0.057366641907530616	3.492390318178191
>500	0.004952515848132139	1.2181734194115497
>1k	0.0012381289620330348	0.8034667282544771
>5k	0.0	0.0
>10k+	4.127096540110116E-4	4.701181896573815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32070	4.61379101986793	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2484	0.3573637946165245	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1757	0.25277302219856423	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1240	0.1783941647844164	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	922	0.13264469349292898	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	789	0.1135104806571811	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	783	0.11264728308564359	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	762	0.10962609158526235	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	739	0.10631716756103525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	719	0.10343984232257693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	705	0.10142571465565611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1887525356428664	0.0
2	0.0	0.0	0.0	1.0260541800342402	0.0
3	0.0	0.0	0.0	1.467148139089902	0.0
4	1.4386626192291647E-4	0.0	0.0	1.9093930282409473	0.0
5	1.4386626192291647E-4	0.0	0.0	2.953718223539398	0.0
6	1.4386626192291647E-4	0.0	0.0	3.46832784243767	0.0
7	1.4386626192291647E-4	0.0	0.0	4.035448646937807	0.0
8	1.4386626192291647E-4	0.0	0.0	4.849875555683437	0.0
9	1.4386626192291647E-4	0.0	0.0	5.158324821246169	0.0
10	1.4386626192291647E-4	0.0	0.0	5.831906659569264	0.0
11	1.4386626192291647E-4	0.0	0.0	6.895941532751155	0.0
12	1.4386626192291647E-4	0.0	0.0	7.771511602814024	0.0
13	1.4386626192291647E-4	0.0	0.0	8.097080953545584	0.0
14	1.4386626192291647E-4	0.0	0.0	8.249866923707721	0.0
15	1.4386626192291647E-4	0.0	0.0	8.535153721100865	0.0
16	1.4386626192291647E-4	0.0	0.0	9.122128069746363	0.0
17	1.4386626192291647E-4	0.0	0.0	9.800457494712916	0.0
18	1.4386626192291647E-4	0.0	0.0	10.605389230171632	0.0
19	1.4386626192291647E-4	0.0	0.0	11.024040052367319	0.0
20	1.4386626192291647E-4	0.0	0.0	11.463839215065676	0.0
21	1.4386626192291647E-4	0.0	0.0	11.977441770130486	0.0
22	1.4386626192291647E-4	0.0	0.0	12.516652519817578	0.0
23	1.4386626192291647E-4	0.0	0.0	13.079745068983872	0.0
24	1.4386626192291647E-4	0.0	0.0	13.49638176351264	0.0
25	1.4386626192291647E-4	0.0	0.0	13.871009509559913	0.0
26	1.4386626192291647E-4	0.0	0.0	14.211253219007611	0.0
27	1.4386626192291647E-4	0.0	0.0	14.537398034786863	0.0
28	1.4386626192291647E-4	0.0	0.0	14.900660346142226	0.0
29	1.4386626192291647E-4	0.0	0.0	15.25255722280568	0.0
30	1.4386626192291647E-4	0.0	0.0	15.67825749183559	0.0
31	1.4386626192291647E-4	0.0	0.0	16.072019450718614	0.0
32	1.4386626192291647E-4	0.0	0.0	16.439453883669742	0.0
33	1.4386626192291647E-4	0.0	0.0	16.786027708642045	0.0
34	1.4386626192291647E-4	0.0	0.0	17.132169934828582	0.0
35	1.4386626192291647E-4	0.0	0.0	17.497590240112793	0.0
36	1.4386626192291647E-4	0.0	0.0	17.859270022587005	0.0
37	1.4386626192291647E-4	0.0	0.0	18.20598771382123	0.0
38	1.4386626192291647E-4	0.0	0.0	18.534865988577018	0.0
39	1.4386626192291647E-4	0.0	0.0	18.862449466975498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGA	35	1.2109922E-7	45.000004	4
TTGTGCG	65	0.0	45.000004	1
CGGTCTA	30	2.1642572E-6	45.000004	31
TAGCTAG	60	0.0	45.000004	1
CAACGCG	20	7.03136E-4	45.0	17
CGTAGCT	20	7.03136E-4	45.0	38
GAAGTAC	20	7.03136E-4	45.0	45
ACGTCAT	20	7.03136E-4	45.0	22
AGTTACG	20	7.03136E-4	45.0	1
TTACGGT	20	7.03136E-4	45.0	35
CCGCGAA	20	7.03136E-4	45.0	32
TAAGCGT	20	7.03136E-4	45.0	16
CGTTTTT	19815	0.0	44.40954	1
TACGGGT	75	0.0	42.000004	4
ACAACGG	65	0.0	41.538464	2
GTTTTTT	21990	0.0	40.21146	2
TGCACGG	40	3.4555887E-7	39.375	2
TCGTGTC	35	6.2447252E-6	38.57143	32
CTACGAA	95	0.0	37.894737	11
TACGAAT	95	0.0	37.894737	12
>>END_MODULE