FastQCFastQC Report
Sat 14 Jan 2017
SRR2934881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418850
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1036514.2851354982739736No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC30060.12427393182710793TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA29800.12319904086652748No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG26840.11096182069991938TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA26050.10769580585815575No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC24860.1027761126154991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT690400.044.504641
TATAGCG603.6379788E-1241.2499961
GTTTTTT767800.040.2145732
CCCTCGT4000.038.812514
CGCATAC301.14029484E-437.49999641
TATAACG601.5643309E-1037.4999961
ATAGGGA15500.036.290324
CGAACCG250.00210756836.01
GTATACG504.8827133E-836.01
CGCGTAG250.00210756836.01
CGGACGT250.00210756836.011
TACGGGA8050.035.7763984
AATTGCG1400.035.3571431
CGTGTAC4350.035.17241318
TACGGGC2700.035.04
TAGGGCG4450.034.8876385
TATCGGG2200.034.7727283
TCGTGTA4450.034.38202317
TGCGGGA14100.034.1489374
TACTCCT4500.034.00000422
Produced by FastQC (version 0.11.5)