##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418850
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75086632077227	34.0	31.0	34.0	31.0	34.0
2	32.89197800607727	34.0	31.0	34.0	31.0	34.0
3	32.926844574901295	34.0	33.0	34.0	31.0	34.0
4	36.296124191247905	37.0	37.0	37.0	35.0	37.0
5	36.19188870744362	37.0	37.0	37.0	35.0	37.0
6	36.19270810509126	37.0	37.0	37.0	35.0	37.0
7	36.30255203919218	37.0	37.0	37.0	35.0	37.0
8	36.322604130061805	37.0	37.0	37.0	35.0	37.0
9	38.102194017818384	39.0	39.0	39.0	37.0	39.0
10	37.75625317816318	39.0	38.0	39.0	35.0	39.0
11	37.61013787543668	39.0	37.0	39.0	35.0	39.0
12	37.558348388697105	39.0	37.0	39.0	35.0	39.0
13	37.57378588998905	39.0	37.0	39.0	35.0	39.0
14	38.93387105442669	40.0	38.0	41.0	35.0	41.0
15	38.957580668499496	41.0	38.0	41.0	35.0	41.0
16	39.01808007937656	41.0	38.0	41.0	35.0	41.0
17	38.99790437604647	41.0	38.0	41.0	35.0	41.0
18	38.45620191413275	39.0	38.0	41.0	35.0	41.0
19	37.949282510283815	38.0	37.0	41.0	35.0	41.0
20	37.36675196891085	38.0	35.0	41.0	34.0	41.0
21	37.309605390991585	38.0	35.0	41.0	34.0	41.0
22	37.13160510159787	38.0	35.0	41.0	34.0	41.0
23	37.037817557930424	38.0	35.0	41.0	34.0	41.0
24	36.86319738718813	38.0	35.0	40.0	33.0	41.0
25	36.71560948384563	37.0	35.0	40.0	33.0	41.0
26	36.838116460301386	38.0	35.0	40.0	33.0	41.0
27	36.851415755420966	38.0	35.0	40.0	34.0	41.0
28	36.77099985530314	38.0	35.0	40.0	33.0	41.0
29	36.61776050602559	38.0	35.0	40.0	33.0	41.0
30	36.31809413564297	38.0	35.0	41.0	33.0	41.0
31	35.817412820141804	38.0	35.0	41.0	31.0	41.0
32	35.06616449965893	38.0	35.0	41.0	23.0	41.0
33	34.068937304917625	38.0	34.0	41.0	16.0	41.0
34	33.233623416086154	38.0	33.0	41.0	12.0	41.0
35	32.70873431589391	38.0	33.0	41.0	10.0	41.0
36	32.43679310416107	38.0	31.0	41.0	8.0	41.0
37	32.1918577009736	37.0	31.0	41.0	8.0	41.0
38	32.05125824255328	37.0	30.0	41.0	7.0	41.0
39	31.976902660355126	37.0	30.0	41.0	7.0	41.0
40	31.8672216135767	37.0	29.0	40.0	7.0	41.0
41	31.718929243235422	37.0	29.0	40.0	7.0	41.0
42	31.587508940198855	37.0	28.0	40.0	7.0	41.0
43	31.48118651425264	37.0	27.0	40.0	7.0	41.0
44	31.3822221303512	36.0	26.0	40.0	7.0	41.0
45	31.340027699113215	36.0	25.0	40.0	7.0	41.0
46	31.311935010438845	36.0	25.0	40.0	7.0	41.0
47	31.325173946296793	36.0	26.0	40.0	7.0	41.0
48	31.26775451144139	35.0	25.0	40.0	7.0	41.0
49	31.134494491183826	35.0	26.0	40.0	7.0	41.0
50	31.066997126733778	35.0	25.0	40.0	7.0	41.0
51	30.55435186142175	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	23.0
10	34.0
11	21.0
12	37.0
13	41.0
14	69.0
15	125.0
16	225.0
17	466.0
18	902.0
19	1590.0
20	2788.0
21	4556.0
22	6719.0
23	11504.0
24	22225.0
25	49188.0
26	92743.0
27	124223.0
28	120233.0
29	97749.0
30	75584.0
31	64568.0
32	59505.0
33	67696.0
34	120974.0
35	145202.0
36	160715.0
37	199251.0
38	382327.0
39	607108.0
40	447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.637224300804103	15.4081485003204	18.294189387518863	38.66043781135664
2	51.97159807346466	18.097153606052462	17.753064472786654	12.17818384769622
3	22.77731153233975	17.58459598569568	47.271513322446616	12.366579159517952
4	21.051326043367716	18.832172313289373	46.63596337102342	13.480538272319489
5	18.87607747483308	21.748434173264155	45.36486346817703	14.010624883725736
6	19.85273993840048	24.49705438534841	44.43487607747483	11.215329598776277
7	58.96533476652128	4.503503731111892	33.000475432540256	3.5306860698265705
8	60.5872212001571	3.8493912396386714	32.668788887281146	2.8945986729230833
9	55.704032908200176	5.920085991276847	34.076069206441076	4.299811894081898
10	29.87291481489137	21.96920023978337	38.606362527647434	9.551522417677823
11	22.250987039295534	18.037290447940137	46.05882960911177	13.65289290365256
12	21.296773260020256	16.271492651466605	47.843768733075635	14.587965355437502
13	18.753167827686713	16.481675176220104	48.90038654732621	15.864770448766977
14	15.265890815883582	18.43392521239432	49.01291936250698	17.28726460921512
15	15.24720424995349	19.238357070508712	50.592182235359786	14.92225644417802
16	17.747359282303574	19.07964528598301	49.02325485251256	14.14974057920086
17	17.41860801620605	19.138433553134753	47.66116129565703	15.78179713500217
18	17.303511999503897	19.56272608884387	47.964900675941045	15.168861235711184
19	18.20253426214937	20.24619137193294	46.585567521756204	14.965706844161481
20	19.403063439237656	19.651156541331623	47.090683589308966	13.855096430121753
21	18.862765363705893	19.81470533517994	47.18585278128036	14.136676519833804
22	18.046344337185026	18.643280897947374	47.37817557930421	15.932199185563386
23	17.71469913388594	18.812576224238793	48.086446038406685	15.38627860346859
24	17.832399694069494	17.821030655063357	48.407838435620235	15.938731215246914
25	17.2779626682101	19.283337123013002	46.70765032970213	16.731049879074767
26	16.216549186596936	20.3403683568638	46.86202120842549	16.581061248113773
27	16.05527420054985	20.18620418794055	47.18647291068069	16.572048700828905
28	15.271844058126796	20.78446369142361	47.49521466812742	16.448477582322177
29	16.354424623271388	19.443619902019556	47.190193687082704	17.01176178762635
30	16.686731297930834	20.265787460983525	46.55588399446018	16.49159724662546
31	17.595840998821753	20.782355251462473	44.76631457097381	16.855489178741966
32	18.168303119250883	21.50832006945449	43.41571407900449	16.907662732290138
33	18.032660148417637	23.944643115530106	41.101845918515	16.920850817537257
34	17.88349835665709	24.56766645306654	39.98755607003328	17.56127912024309
35	18.060069867912436	25.62866651507948	38.07160427475866	18.239659342249418
36	17.72949955557393	28.502676891911445	36.22853835500341	17.539285197511216
37	18.851892428220022	28.278438100750357	35.735576823697215	17.13409264733241
38	18.184798561299793	27.656241602414372	35.531223515306856	18.627736320978975
39	19.283213097132933	26.92490232961945	35.1837029993592	18.60818157388842
40	19.426173594890134	26.629100605659715	36.21344853959526	17.73127725985489
41	17.683775347789236	27.67769807966596	35.622837298716334	19.015689273828475
42	17.699361266717656	28.004010170122168	34.720300969468965	19.576327593691218
43	18.298034189800937	26.826549806726334	35.082415197304506	19.79300080616822
44	18.1696260619716	26.669863778241726	34.81840544060194	20.342104719184736
45	18.398164416975007	25.88225809785642	34.879963619075184	20.839613866093394
46	17.70047749963826	26.524877524443436	35.924840316679415	19.849804659238895
47	17.46081815738884	25.59840420034314	37.73950430989933	19.201273332368686
48	17.28933170721624	24.48659486946276	38.34995969158898	19.87411373173202
49	17.446141761580915	24.52359592368274	38.33334022366001	19.69692209107634
50	16.79872666763131	23.92087148851727	38.8774004175538	20.40300142629762
51	16.376955991483555	23.977633999627923	38.05366186410898	21.591748144779544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	615.0
1	1399.5
2	2184.0
3	114513.0
4	226842.0
5	155945.0
6	85048.0
7	78338.5
8	71629.0
9	67876.5
10	64124.0
11	59965.0
12	55806.0
13	51601.5
14	47397.0
15	43343.0
16	39289.0
17	35605.0
18	31921.0
19	29111.0
20	26301.0
21	24364.5
22	22428.0
23	21331.0
24	20234.0
25	20608.0
26	22798.5
27	24615.0
28	26897.0
29	29179.0
30	33991.0
31	38803.0
32	41587.5
33	44372.0
34	48896.5
35	53421.0
36	57632.5
37	61844.0
38	65163.5
39	68483.0
40	76574.0
41	84665.0
42	92756.5
43	100848.0
44	113850.0
45	126852.0
46	135750.5
47	144649.0
48	144483.5
49	144318.0
50	130449.5
51	116581.0
52	107413.0
53	98245.0
54	94313.0
55	90381.0
56	88220.0
57	86059.0
58	85235.5
59	84412.0
60	85347.5
61	86283.0
62	79994.0
63	73705.0
64	63426.0
65	53147.0
66	43396.5
67	33646.0
68	28145.5
69	22645.0
70	18660.0
71	14675.0
72	12368.5
73	10062.0
74	7875.5
75	4301.0
76	2913.0
77	2263.5
78	1614.0
79	1335.5
80	1057.0
81	838.0
82	619.0
83	371.5
84	124.0
85	109.0
86	94.0
87	60.0
88	26.0
89	21.0
90	16.0
91	10.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2418850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.29241371439889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15022299843858	23.673588869412324
2	8.529557261078898	5.1676175470651415
3	2.9568476959800543	2.6871016109128485
4	1.4529609085352047	1.7605477180878923
5	0.9022202661098161	1.3665214781256805
6	0.6722272022665967	1.2218030712679593
7	0.4931458231195833	1.0457004106826338
8	0.43301350600091226	1.0493619414161584
9	0.360991920343215	0.9841784938732797
>10	5.509091458075575	40.42578783156433
>50	0.4639197542122986	8.624278252670251
>100	0.06531265893067044	4.160444742861421
>500	0.006153280853055943	1.2697498046269668
>1k	0.0041954187634472345	2.1723384456499595
>5k	0.0	0.0
>10k+	1.398472921149078E-4	4.390979781783263
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103651	4.2851354982739736	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	3006	0.12427393182710793	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2980	0.12319904086652748	No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	2684	0.11096182069991938	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	2605	0.10769580585815575	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2486	0.1027761126154991	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.893937201562726E-4	0.0	0.0	0.0725964817992021	0.0
2	2.893937201562726E-4	0.0	0.0	0.3499596915889782	0.0
3	2.893937201562726E-4	0.0	0.0	0.5904458730388408	0.0
4	2.893937201562726E-4	0.0	0.0	0.8109225458378981	0.0
5	2.893937201562726E-4	0.0	0.0	1.3751162742625629	0.0
6	2.893937201562726E-4	0.0	0.0	1.8522438348802117	0.0
7	3.307356801785973E-4	0.0	0.0	2.2602062963805114	0.0
8	3.307356801785973E-4	0.0	0.0	2.7974450668706203	0.0
9	3.307356801785973E-4	0.0	0.0	3.0895673563883665	0.0
10	3.720776402009219E-4	0.0	0.0	3.5365566281497407	0.0
11	3.720776402009219E-4	0.0	0.0	4.153585381482936	0.0
12	3.720776402009219E-4	0.0	0.0	4.655311408313868	0.0
13	4.134196002232466E-4	0.0	0.0	4.86714761146826	0.0
14	4.134196002232466E-4	0.0	0.0	4.977613328647911	0.0
15	4.134196002232466E-4	0.0	0.0	5.134836802612812	0.0
16	4.134196002232466E-4	0.0	0.0	5.42059243028712	0.0
17	4.134196002232466E-4	0.0	0.0	5.75848026954958	0.0
18	4.134196002232466E-4	0.0	0.0	6.119312896624429	0.0
19	4.134196002232466E-4	0.0	0.0	6.372201666080989	0.0
20	4.134196002232466E-4	0.0	0.0	6.638195836864626	0.0
21	4.5476156024557127E-4	0.0	0.0	6.955908799636191	0.0
22	4.5476156024557127E-4	0.0	0.0	7.299212435661575	0.0
23	4.5476156024557127E-4	0.0	0.0	7.628418463319346	0.0
24	4.5476156024557127E-4	0.0	0.0	7.910329288711578	0.0
25	4.5476156024557127E-4	0.0	0.0	8.1653678400893	0.0
26	4.5476156024557127E-4	0.0	0.0	8.412675444942845	0.0
27	4.5476156024557127E-4	0.0	0.0	8.644645182628109	0.0
28	4.5476156024557127E-4	0.0	0.0	8.887157120119065	0.0
29	4.5476156024557127E-4	0.0	0.0	9.14174090993654	0.0
30	4.5476156024557127E-4	0.0	0.0	9.431837443413192	0.0
31	4.5476156024557127E-4	0.0	0.0	9.697500878516651	0.0
32	4.5476156024557127E-4	0.0	0.0	9.968662794303079	0.0
33	4.5476156024557127E-4	0.0	0.0	10.230316059284371	0.0
34	4.96103520267896E-4	0.0	0.0	10.484114351861422	0.0
35	4.96103520267896E-4	0.0	0.0	10.75118341360564	0.0
36	4.96103520267896E-4	0.0	0.0	11.041445314922381	0.0
37	5.374454802902206E-4	0.0	0.0	11.338445955722761	0.0
38	5.374454802902206E-4	0.0	0.0	11.64842797197015	0.0
39	5.374454802902206E-4	0.0	0.0	11.963205655580131	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	69040	0.0	44.50464	1
TATAGCG	60	3.6379788E-12	41.249996	1
GTTTTTT	76780	0.0	40.214573	2
CCCTCGT	400	0.0	38.8125	14
CGCATAC	30	1.14029484E-4	37.499996	41
TATAACG	60	1.5643309E-10	37.499996	1
ATAGGGA	1550	0.0	36.29032	4
CGAACCG	25	0.002107568	36.0	1
GTATACG	50	4.8827133E-8	36.0	1
CGCGTAG	25	0.002107568	36.0	1
CGGACGT	25	0.002107568	36.0	11
TACGGGA	805	0.0	35.776398	4
AATTGCG	140	0.0	35.357143	1
CGTGTAC	435	0.0	35.172413	18
TACGGGC	270	0.0	35.0	4
TAGGGCG	445	0.0	34.887638	5
TATCGGG	220	0.0	34.772728	3
TCGTGTA	445	0.0	34.382023	17
TGCGGGA	1410	0.0	34.148937	4
TACTCCT	450	0.0	34.000004	22
>>END_MODULE